FastQCFastQC Report
Fri 17 Jun 2016
SRR1525832_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525832_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238
Sequences flagged as poor quality0
Sequence length40
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT80.6462035541195477No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG40.32310177705977383No Hit
GAATTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT40.32310177705977383No Hit
GAATTAGGGGGGCTGGTGAGATGGCCCAGTGGGTAAGAGC40.32310177705977383No Hit
GAATTAGGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAG30.24232633279483037No Hit
GAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.16155088852988692No Hit
GAATTAGGGTCATCGATTTTTTTTTTTTTTTTTTTTTTTT20.16155088852988692No Hit
GAATTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC20.16155088852988692No Hit
GAATTAGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT20.16155088852988692No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGCGC20.16155088852988692No Hit
GAATTAGGGTACCTGGTTGATCCTCCCAGTACCATCTGCT20.16155088852988692No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCCGTGGTCGAGCC20.16155088852988692No Hit
GAATTAGGGTACCTGGTTGATCCTGCCAGTCGCATATGCT20.16155088852988692No Hit
GAATTAGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT20.16155088852988692No Hit
GAATTAGGGGGGCTGGTGCGATGGCCCAGTGGGTAAGAGC20.16155088852988692No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG200.001914334433.6666686
TTAGGGT251.1253028E-433.6666684
TTAGGGG650.033.6666684
TAGGGGG251.1253028E-433.6666685
ATTAGGG1150.032.2028963
AATTAGG1150.032.2028962
GAATTAG1150.032.2028961
TTAGGGA250.005728470626.9333324
Produced by FastQC (version 0.11.5)