FastQCFastQC Report
Fri 17 Jun 2016
SRR1525831_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525831_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3302
Sequences flagged as poor quality0
Sequence length40
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT812.453058752271351No Hit
GAATTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT601.8170805572380373No Hit
GAATTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG371.1205330102967896No Hit
GAATTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.6965475469412478No Hit
GAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.6056935190793459No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC150.4542701393095093No Hit
GAATTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.27256208358570566No Hit
GAATTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.24227740763173833No Hit
GAATTAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT80.24227740763173833No Hit
GAATTAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT80.24227740763173833No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG60.18170805572380377No Hit
GAATTAGGGACTAATCCTAGCCCTAGCCCTACACAAATAT60.18170805572380377No Hit
GAATTAGGGGAGTGCAGTGCTGAATTAGGGGTAGAAGGTC50.15142337976983647No Hit
GAATTAGGGGGGCAATTTGTTTGGTGAAATGGCCCAGCTG50.15142337976983647No Hit
GAATTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT50.15142337976983647No Hit
GAATTAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG50.15142337976983647No Hit
GAATTAGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT50.15142337976983647No Hit
GAATTAGGGTACCTGGTTGATCCTGCCAGTACCATATGCT40.12113870381586916No Hit
GAATTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC40.12113870381586916No Hit
GAATTAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCT40.12113870381586916No Hit
GAATTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCG40.12113870381586916No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG40.12113870381586916No Hit
GAATTAGGGCGACAGCAAAGCATCAAACCTGGGCCTGAGC40.12113870381586916No Hit
GAATTAGGGGCTGAATTAGGGTCACAGATCCTCTTCTGAG40.12113870381586916No Hit
GAATTAGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTA40.12113870381586916No Hit
GAATTAGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT40.12113870381586916No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCCA200.002156911934.73437522
ATCCTGC200.002333159834.220
CTCTTCT251.5638264E-434.219
GATCCTG200.002333159834.219
TCTTCTG251.5638264E-434.220
TCCTGCC200.002333159834.221
CTTCTGA251.5638264E-434.221
GGGTCAC251.7169006E-433.681827
GTCACAG251.7169006E-433.681829
AGGGTCA251.7169006E-433.681826
AGGGTAA251.7169006E-433.681826
TTAGGGT750.033.681824
TAGGGTC251.7169006E-433.681825
TAGGGAT357.732833E-733.681825
TAGGGAG301.158253E-533.681825
TCACAGA251.7169006E-433.6818210
CACAGAT251.7169006E-433.6818211
ATCCTCT200.002520626933.68181616
TCCTCTT200.002520626933.68181617
GTTGATC200.002520626933.68181616
Produced by FastQC (version 0.11.5)