##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525831_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3302
Sequences flagged as poor quality	0
Sequence length	40
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	18.937007874015748	25.0	4.0	33.0	4.0	35.0
2	13.51847365233192	4.0	4.0	24.0	4.0	33.0
3	13.789218655360388	4.0	4.0	25.0	4.0	33.0
4	14.942761962447001	4.0	4.0	29.0	4.0	35.0
5	14.347970926711085	4.0	4.0	28.0	4.0	35.0
6	11.06602059357965	4.0	4.0	15.0	4.0	31.0
7	13.060266505148395	4.0	4.0	26.0	4.0	33.0
8	12.79588128407026	4.0	4.0	25.0	4.0	33.0
9	12.648697758933979	4.0	4.0	25.0	4.0	33.0
10	11.513022410660206	4.0	4.0	20.0	4.0	32.0
11	11.242580254391278	4.0	4.0	19.0	4.0	32.0
12	10.605087825560267	4.0	4.0	17.0	4.0	31.0
13	9.960629921259843	4.0	4.0	13.0	4.0	30.0
14	10.0732889158086	4.0	4.0	13.0	4.0	30.0
15	10.136281041792852	4.0	4.0	12.0	4.0	31.0
16	8.702604482132042	4.0	4.0	4.0	4.0	28.0
17	8.236826165960025	4.0	4.0	4.0	4.0	26.0
18	8.071471835251362	4.0	4.0	4.0	4.0	25.0
19	7.742277407631739	4.0	4.0	4.0	4.0	23.0
20	7.929133858267717	4.0	4.0	4.0	4.0	25.0
21	7.4306480920654145	4.0	4.0	4.0	4.0	23.0
22	7.145063597819504	4.0	4.0	4.0	4.0	21.0
23	7.074803149606299	4.0	4.0	4.0	4.0	20.0
24	7.059055118110236	4.0	4.0	4.0	4.0	20.0
25	7.080860084797092	4.0	4.0	4.0	4.0	21.0
26	6.973652331920048	4.0	4.0	4.0	4.0	20.0
27	6.838885523924894	4.0	4.0	4.0	4.0	18.0
28	7.023924894003634	4.0	4.0	4.0	4.0	21.0
29	6.682010902483343	4.0	4.0	4.0	4.0	17.0
30	6.725923682616596	4.0	4.0	4.0	4.0	18.0
31	7.074803149606299	4.0	4.0	4.0	4.0	22.0
32	7.0427013930950935	4.0	4.0	4.0	4.0	22.0
33	6.9439733494851605	4.0	4.0	4.0	4.0	21.0
34	6.9897032101756515	4.0	4.0	4.0	4.0	22.0
35	6.859176256814052	4.0	4.0	4.0	4.0	20.0
36	6.73167777104785	4.0	4.0	4.0	4.0	17.0
37	6.6465778316172015	4.0	4.0	4.0	4.0	17.0
38	6.577831617201696	4.0	4.0	4.0	4.0	15.0
39	6.385221078134464	4.0	4.0	4.0	4.0	4.0
40	6.467898243488794	4.0	4.0	4.0	4.0	4.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	14.11165991219183
1	2	13.336659912191823
1	3	-3.654972982100647
1	4	17.587884160756502
1	5	17.512901046943604
1	6	-6.923826409996623
1	7	-8.431425194191155
1	8	-8.376709726443769
1	9	-8.43113812901047
1	10	-6.357784532252621
1	11	-7.1415864572779455
1	12	-6.205179837892606
1	13	-6.340961668355288
1	14	-5.37237841945289
1	15	-5.504871664978051
1	16	-3.870322526173589
1	17	-3.3879896994258694
1	18	-2.7714496791624477
1	19	-3.047699257007767
1	20	-2.9955378250591016
1	21	-2.372441742654508
1	22	-1.9951578858493741
1	23	-1.9175320837554883
1	24	-1.969942586963863
1	25	-2.302777777777778
1	26	-2.0184734886862543
1	27	-1.909397163120568
1	28	-2.1053444782168205
1	29	-1.9258569739952716
1	30	-2.0609506923336722
1	31	-2.0933975008443095
1	32	-2.3535714285714286
1	33	-2.1365417088821346
1	34	-2.0899358324890223
1	35	-1.9615585950692331
1	36	-1.8922534616683562
1	37	-1.8341354272205335
1	38	-1.739146403242147
1	39	-1.3107100641675107
1	40	-1.6308975008443083
2	1	-14.88834008780817
2	2	-8.996673421141509
2	3	-9.321639648767313
2	4	-11.07878250591016
2	5	-10.153765619723066
2	6	-6.923826409996623
2	7	-8.431425194191155
2	8	-8.376709726443769
2	9	-8.43113812901047
2	10	-6.357784532252621
2	11	-7.1415864572779455
2	12	-6.205179837892606
2	13	-6.340961668355288
2	14	-5.37237841945289
2	15	-5.504871664978051
2	16	-3.870322526173589
2	17	-3.3879896994258694
2	18	-2.7714496791624477
2	19	-3.047699257007767
2	20	-2.9955378250591016
2	21	-2.372441742654508
2	22	-1.9951578858493741
2	23	-1.9175320837554883
2	24	-1.969942586963863
2	25	-2.302777777777778
2	26	-2.0184734886862543
2	27	-1.909397163120568
2	28	-2.1053444782168205
2	29	-1.9258569739952716
2	30	-2.0609506923336722
2	31	-2.0933975008443095
2	32	-2.3535714285714286
2	33	-2.1365417088821346
2	34	-2.0899358324890223
2	35	-1.9615585950692331
2	36	-1.8922534616683562
2	37	-1.8341354272205335
2	38	-1.739146403242147
2	39	-1.3107100641675107
2	40	-1.6308975008443083
3	1	-0.38834008780817086
3	2	5.003326578858491
3	3	-1.821639648767313
3	4	2.9212174940898397
3	5	2.8462343802769343
3	6	-2.4238264099966234
3	7	5.568574805808845
3	8	-3.876709726443769
3	9	5.0688618709895295
3	10	-6.357784532252621
3	11	-7.1415864572779455
3	12	-6.205179837892606
3	13	-6.340961668355288
3	14	-5.37237841945289
3	15	-5.504871664978051
3	16	-3.870322526173589
3	17	-3.3879896994258694
3	18	-2.7714496791624477
3	19	-3.047699257007767
3	20	-2.9955378250591016
3	21	-2.372441742654508
3	22	-1.9951578858493741
3	23	-1.9175320837554883
3	24	-1.969942586963863
3	25	-2.302777777777778
3	26	-2.0184734886862543
3	27	-1.909397163120568
3	28	-2.1053444782168205
3	29	-1.9258569739952716
3	30	-2.0609506923336722
3	31	-2.0933975008443095
3	32	-2.3535714285714286
3	33	-2.1365417088821346
3	34	-2.0899358324890223
3	35	-1.9615585950692331
3	36	-1.8922534616683562
3	37	-1.8341354272205335
3	38	-1.739146403242147
3	39	-1.3107100641675107
3	40	-1.6308975008443083
4	1	-14.88834008780817
4	2	-8.996673421141509
4	3	-9.321639648767313
4	4	-11.07878250591016
4	5	-10.153765619723066
4	6	-6.923826409996623
4	7	-8.431425194191155
4	8	-8.376709726443769
4	9	-8.43113812901047
4	10	-6.357784532252621
4	11	-7.1415864572779455
4	12	-6.205179837892606
4	13	-6.340961668355288
4	14	-5.37237841945289
4	15	-5.504871664978051
4	16	-3.870322526173589
4	17	-3.3879896994258694
4	18	-2.7714496791624477
4	19	-3.047699257007767
4	20	-2.9955378250591016
4	21	-2.372441742654508
4	22	-1.9951578858493741
4	23	-1.9175320837554883
4	24	-1.969942586963863
4	25	-2.302777777777778
4	26	-2.0184734886862543
4	27	-1.909397163120568
4	28	-2.1053444782168205
4	29	-1.9258569739952716
4	30	-2.0609506923336722
4	31	-2.0933975008443095
4	32	-2.3535714285714286
4	33	-2.1365417088821346
4	34	-2.0899358324890223
4	35	-1.9615585950692331
4	36	-1.8922534616683562
4	37	-1.8341354272205335
4	38	-1.739146403242147
4	39	-1.3107100641675107
4	40	-1.6308975008443083
5	1	0.11165991219182914
5	2	3.5033265788584913
5	3	5.678360351232687
5	4	4.17121749408984
5	5	4.096234380276934
5	6	-1.4238264099966234
5	7	4.068574805808845
5	8	6.123290273556231
5	9	7.0688618709895295
5	10	9.142215467747379
5	11	0.3584135427220545
5	12	0.5448201621073938
5	13	0.15903833164471237
5	14	0.8776215805471104
5	15	-0.2548716649780509
5	16	0.879677473826411
5	17	3.1120103005741306
5	18	1.4785503208375523
5	19	1.9523007429922332
5	20	3.0044621749408984
5	21	0.8775582573454921
5	22	5.004842114150626
5	23	4.582467916244512
5	24	1.280057413036137
5	25	1.697222222222222
5	26	-0.018473488686254314
5	27	4.590602836879432
5	28	0.8946555217831795
5	29	3.0741430260047284
5	30	2.9390493076663278
5	31	-1.8433975008443095
5	32	1.3964285714285714
5	33	4.363458291117865
5	34	-2.0899358324890223
5	35	-1.9615585950692331
5	36	-1.8922534616683562
5	37	-1.8341354272205335
5	38	-1.739146403242147
5	39	-1.3107100641675107
5	40	-1.6308975008443083
6	1	12.11165991219183
6	2	6.003326578858491
6	3	17.678360351232687
6	4	15.92121749408984
6	5	23.846234380276933
6	6	27.07617359000338
6	7	14.568574805808845
6	8	22.62329027355623
6	9	22.56886187098953
6	10	9.642215467747379
6	11	23.858413542722055
6	12	25.794820162107392
6	13	11.659038331644712
6	14	12.62762158054711
6	15	10.495128335021949
6	16	16.129677473826412
6	17	23.61201030057413
6	18	-2.7714496791624477
6	19	-3.047699257007767
6	20	-2.9955378250591016
6	21	-2.372441742654508
6	22	-1.9951578858493741
6	23	-1.9175320837554883
6	24	-1.969942586963863
6	25	-2.302777777777778
6	26	-2.0184734886862543
6	27	-1.909397163120568
6	28	-2.1053444782168205
6	29	-1.9258569739952716
6	30	-2.0609506923336722
6	31	-2.0933975008443095
6	32	-2.3535714285714286
6	33	-2.1365417088821346
6	34	-2.0899358324890223
6	35	-1.9615585950692331
6	36	-1.8922534616683562
6	37	-1.8341354272205335
6	38	-1.739146403242147
6	39	-1.3107100641675107
6	40	-1.6308975008443083
8	1	-14.88834008780817
8	2	-8.996673421141509
8	3	-9.321639648767313
8	4	-11.07878250591016
8	5	-10.153765619723066
8	6	-6.923826409996623
8	7	-8.431425194191155
8	8	-8.376709726443769
8	9	-8.43113812901047
8	10	-6.357784532252621
8	11	-7.1415864572779455
8	12	-6.205179837892606
8	13	-6.340961668355288
8	14	-5.37237841945289
8	15	-5.504871664978051
8	16	-3.870322526173589
8	17	-3.3879896994258694
8	18	-2.7714496791624477
8	19	-3.047699257007767
8	20	-2.9955378250591016
8	21	-2.372441742654508
8	22	-1.9951578858493741
8	23	-1.9175320837554883
8	24	-1.969942586963863
8	25	-2.302777777777778
8	26	-2.0184734886862543
8	27	-1.909397163120568
8	28	-2.1053444782168205
8	29	-1.9258569739952716
8	30	-2.0609506923336722
8	31	-2.0933975008443095
8	32	-2.3535714285714286
8	33	-2.1365417088821346
8	34	-2.0899358324890223
8	35	-1.9615585950692331
8	36	-1.8922534616683562
8	37	-1.8341354272205335
8	38	-1.739146403242147
8	39	-1.3107100641675107
8	40	-1.6308975008443083
9	1	16.11165991219183
9	2	10.503326578858491
9	3	11.178360351232687
9	4	19.921217494089838
9	5	20.846234380276933
9	6	2.5761735900033766
9	7	16.568574805808844
9	8	-2.876709726443769
9	9	2.5688618709895295
9	10	7.142215467747379
9	11	6.3584135427220545
9	12	-3.205179837892606
9	13	6.659038331644712
9	14	0.6276215805471104
9	15	3.995128335021949
9	16	-1.370322526173589
9	17	-0.8879896994258694
9	18	2.2285503208375523
9	19	4.452300742992233
9	20	5.004462174940898
9	21	8.127558257345491
9	22	4.504842114150626
9	23	2.5824679162445117
9	24	8.530057413036136
9	25	10.197222222222223
9	26	1.4815265113137457
9	27	6.090602836879432
9	28	9.894655521783179
9	29	5.074143026004728
9	30	12.439049307666327
9	31	8.40660249915569
9	32	10.646428571428572
9	33	5.363458291117865
9	34	10.910064167510978
9	35	12.538441404930767
9	36	2.107746538331644
9	37	8.665864572779466
9	38	11.760853596757853
9	39	10.68928993583249
9	40	13.86910249915569
10	1	-3.388340087808171
10	2	-3.9966734211415087
10	3	-0.821639648767313
10	4	-4.57878250591016
10	5	-5.153765619723066
10	6	-0.9238264099966234
10	7	-1.4314251941911547
10	8	1.623290273556231
10	9	1.0688618709895295
10	10	0.6422154677473788
10	11	5.8584135427220545
10	12	6.294820162107394
10	13	10.159038331644712
10	14	7.62762158054711
10	15	7.495128335021949
10	16	7.129677473826411
10	17	9.61201030057413
10	18	13.728550320837552
10	19	12.952300742992232
10	20	13.504462174940898
10	21	14.127558257345491
10	22	10.004842114150627
10	23	14.08246791624451
10	24	11.030057413036136
10	25	14.197222222222223
10	26	11.481526511313746
10	27	8.590602836879432
10	28	9.894655521783179
10	29	3.0741430260047284
10	30	6.939049307666328
10	31	8.90660249915569
10	32	11.146428571428572
10	33	10.863458291117865
10	34	7.410064167510978
10	35	12.538441404930767
10	36	10.607746538331643
10	37	12.665864572779466
10	38	12.760853596757853
10	39	14.68928993583249
10	40	13.86910249915569
11	1	-14.88834008780817
11	2	-8.996673421141509
11	3	-9.321639648767313
11	4	-11.07878250591016
11	5	-10.153765619723066
11	6	-6.923826409996623
11	7	-8.431425194191155
11	8	-8.376709726443769
11	9	-8.43113812901047
11	10	-6.357784532252621
11	11	-7.1415864572779455
11	12	-6.205179837892606
11	13	-6.340961668355288
11	14	-5.37237841945289
11	15	-5.504871664978051
11	16	-3.870322526173589
11	17	-3.3879896994258694
11	18	-2.7714496791624477
11	19	-3.047699257007767
11	20	-2.9955378250591016
11	21	-2.372441742654508
11	22	-1.9951578858493741
11	23	-1.9175320837554883
11	24	-1.969942586963863
11	25	-2.302777777777778
11	26	-2.0184734886862543
11	27	-1.909397163120568
11	28	-2.1053444782168205
11	29	-1.9258569739952716
11	30	-2.0609506923336722
11	31	-2.0933975008443095
11	32	-2.3535714285714286
11	33	-2.1365417088821346
11	34	-2.0899358324890223
11	35	-1.9615585950692331
11	36	-1.8922534616683562
11	37	-1.8341354272205335
11	38	-1.739146403242147
11	39	-1.3107100641675107
11	40	-1.6308975008443083
12	1	11.11165991219183
12	2	-1.9966734211415087
12	3	1.678360351232687
12	4	14.92121749408984
12	5	-10.153765619723066
12	6	-6.923826409996623
12	7	-8.431425194191155
12	8	-8.376709726443769
12	9	-8.43113812901047
12	10	-6.357784532252621
12	11	-7.1415864572779455
12	12	-6.205179837892606
12	13	-6.340961668355288
12	14	-5.37237841945289
12	15	-5.504871664978051
12	16	-3.870322526173589
12	17	-3.3879896994258694
12	18	-2.7714496791624477
12	19	-3.047699257007767
12	20	-2.9955378250591016
12	21	-2.372441742654508
12	22	-1.9951578858493741
12	23	-1.9175320837554883
12	24	-1.969942586963863
12	25	-2.302777777777778
12	26	-2.0184734886862543
12	27	-1.909397163120568
12	28	-2.1053444782168205
12	29	-1.9258569739952716
12	30	-2.0609506923336722
12	31	-2.0933975008443095
12	32	-2.3535714285714286
12	33	-2.1365417088821346
12	34	-2.0899358324890223
12	35	-1.9615585950692331
12	36	-1.8922534616683562
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114	18	-2.7714496791624477
114	19	-3.047699257007767
114	20	-2.9955378250591016
114	21	-2.372441742654508
114	22	-1.9951578858493741
114	23	-1.9175320837554883
114	24	-1.969942586963863
114	25	-2.302777777777778
114	26	-2.0184734886862543
114	27	-1.909397163120568
114	28	-2.1053444782168205
114	29	-1.9258569739952716
114	30	-2.0609506923336722
114	31	-2.0933975008443095
114	32	-2.3535714285714286
114	33	-2.1365417088821346
114	34	-2.0899358324890223
114	35	-1.9615585950692331
114	36	-1.8922534616683562
114	37	-1.8341354272205335
114	38	-1.739146403242147
114	39	-1.3107100641675107
114	40	-1.6308975008443083
117	1	11.61165991219183
117	2	-8.996673421141509
117	3	-9.321639648767313
117	4	-11.07878250591016
117	5	-10.153765619723066
117	6	-6.923826409996623
117	7	-8.431425194191155
117	8	-8.376709726443769
117	9	-8.43113812901047
117	10	-6.357784532252621
117	11	-7.1415864572779455
117	12	-6.205179837892606
117	13	-6.340961668355288
117	14	-5.37237841945289
117	15	-5.504871664978051
117	16	-3.870322526173589
117	17	-3.3879896994258694
117	18	-2.7714496791624477
117	19	-3.047699257007767
117	20	-2.9955378250591016
117	21	-2.372441742654508
117	22	-1.9951578858493741
117	23	-1.9175320837554883
117	24	-1.969942586963863
117	25	-2.302777777777778
117	26	-2.0184734886862543
117	27	-1.909397163120568
117	28	-2.1053444782168205
117	29	-1.9258569739952716
117	30	-2.0609506923336722
117	31	-2.0933975008443095
117	32	-2.3535714285714286
117	33	-2.1365417088821346
117	34	-2.0899358324890223
117	35	-1.9615585950692331
117	36	-1.8922534616683562
117	37	-1.8341354272205335
117	38	-1.739146403242147
117	39	-1.3107100641675107
117	40	-1.6308975008443083
119	1	14.311659912191832
119	2	2.4033265788584917
119	3	4.478360351232688
119	4	2.521217494089841
119	5	6.446234380276936
119	6	5.476173590003375
119	7	1.9685748058088457
119	8	8.623290273556231
119	9	4.968861870989528
119	10	5.2422154677473785
119	11	3.458413542722054
119	12	5.794820162107394
119	13	10.259038331644714
119	14	-5.37237841945289
119	15	-5.504871664978051
119	16	-3.870322526173589
119	17	-3.3879896994258694
119	18	-2.7714496791624477
119	19	-3.047699257007767
119	20	-2.9955378250591016
119	21	-2.372441742654508
119	22	-1.9951578858493741
119	23	-1.9175320837554883
119	24	-1.969942586963863
119	25	-2.302777777777778
119	26	-2.0184734886862543
119	27	-1.909397163120568
119	28	-2.1053444782168205
119	29	-1.9258569739952716
119	30	-2.0609506923336722
119	31	-2.0933975008443095
119	32	-2.3535714285714286
119	33	-2.1365417088821346
119	34	-2.0899358324890223
119	35	-1.9615585950692331
119	36	-1.8922534616683562
119	37	-1.8341354272205335
119	38	-1.739146403242147
119	39	-1.3107100641675107
119	40	-1.6308975008443083
120	1	16.11165991219183
120	2	3.669993245525159
120	3	19.011693684566023
120	4	17.921217494089838
120	5	9.179567713610266
120	6	8.742840256670044
120	7	6.235241472475513
120	8	7.956623606889563
120	9	10.902195204322862
120	10	11.642215467747379
120	11	14.525080209388722
120	12	15.461486828774062
120	13	14.32570499831138
120	14	13.960954913880443
120	15	13.161795001688617
120	16	5.129677473826411
120	17	7.612010300574131
120	18	7.895216987504218
120	19	7.952300742992233
120	20	7.337795508274232
120	21	-2.372441742654508
120	22	-1.9951578858493741
120	23	-1.9175320837554883
120	24	-1.969942586963863
120	25	-2.302777777777778
120	26	-2.0184734886862543
120	27	-1.909397163120568
120	28	-2.1053444782168205
120	29	-1.9258569739952716
120	30	-2.0609506923336722
120	31	-2.0933975008443095
120	32	-2.3535714285714286
120	33	-2.1365417088821346
120	34	-2.0899358324890223
120	35	-1.9615585950692331
120	36	-1.8922534616683562
120	37	-1.8341354272205335
120	38	-1.739146403242147
120	39	-1.3107100641675107
120	40	-1.6308975008443083
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1743.0
5	63.0
6	120.0
7	107.0
8	72.0
9	95.0
10	119.0
11	133.0
12	83.0
13	71.0
14	74.0
15	59.0
16	35.0
17	37.0
18	37.0
19	29.0
20	33.0
21	49.0
22	58.0
23	70.0
24	46.0
25	38.0
26	30.0
27	38.0
28	22.0
29	14.0
30	9.0
31	9.0
32	3.0
33	5.0
34	0.0
35	0.0
36	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	96.05941194301303	1.5762352227947862	1.7884207335556228	0.5759321006365565
8	94.20861127956337	2.546998180715585	2.2437841115827775	1.0006064281382656
9	93.87693240375872	2.1824795392543193	2.879660503182783	1.0609275538041831
10	39.62378640776699	32.97936893203883	18.5376213592233	8.859223300970873
11	25.95396729254997	35.917625681405205	21.0175651120533	17.11084191399152
12	27.98304058146578	33.73712901271956	20.351302241066023	17.928528164748634
13	17.233009708737864	30.370145631067963	23.78640776699029	28.610436893203882
14	17.474258025439127	40.67231980617807	25.590551181102363	16.262870987280436
15	31.22350090854028	31.798909751665654	23.561477892186552	13.41611144760751
16	24.894003634161113	38.7946698970321	24.19745608721987	12.113870381586917
17	20.933050590730083	31.62677976370797	35.17116025446834	12.269009391093608
18	19.10963052695336	35.3725015142338	28.52816474863719	16.98970321017565
19	22.114510754316875	34.71675249924265	21.963041502574974	21.205695243865495
20	18.413082980012113	36.91701998788613	30.587522713506964	14.08237431859479
21	18.776499091459723	33.01029678982435	29.043004239854636	19.170199878861297
22	18.170805572380374	29.31556632344034	29.92125984251969	22.5923682616596
23	17.17661314753105	33.444410784610724	27.143289912147832	22.23568615571039
24	16.535433070866144	37.15929739551787	26.135675348273775	20.169594185342216
25	21.571601941747574	32.28155339805826	27.336165048543688	18.810679611650485
26	26.125304136253042	31.934306569343068	21.958637469586375	19.98175182481752
27	17.84409257003654	35.74908647990256	23.934226552984168	22.472594397076737
28	22.552288572294636	37.16277659896938	22.03698090330403	18.247953925431947
29	23.8878732480195	35.61852528945765	22.486288848263253	18.0073126142596
30	20.575757575757574	33.151515151515156	30.272727272727273	16.0
31	22.387155407452287	36.716146622235684	24.023023326264767	16.87367464404726
32	25.296442687747035	32.28944968075403	24.779568257829126	17.634539373669806
33	23.265677067555288	32.56588912450773	24.598606482883977	19.569827325053012
34	19.079345850999392	33.525136281041796	31.708055723803756	15.687462144155056
35	18.26165960024228	31.011508176862506	34.28225317989097	16.44457904300424
36	20.642618975447107	33.798120642618976	27.94786298878448	17.61139739314944
37	20.533010296789826	30.890369473046636	32.76801938219261	15.808600847970927
38	23.682616596002422	31.10236220472441	28.07389460932768	17.141126589945486
39	21.471835251362812	33.73712901271956	24.651726226529377	20.13930950938825
40	17.413688673531194	30.860084797092668	33.585705632949725	18.140520896426406
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.5
2	3.0
3	3.0
4	2.5
5	2.0
6	2.5
7	3.0
8	3.0
9	18.5
10	34.0
11	44.0
12	54.0
13	54.0
14	55.0
15	56.0
16	57.5
17	59.0
18	59.0
19	57.0
20	55.0
21	64.5
22	74.0
23	74.0
24	81.0
25	88.0
26	104.5
27	121.0
28	121.0
29	132.5
30	144.0
31	160.5
32	177.0
33	177.0
34	188.5
35	200.0
36	208.0
37	216.0
38	216.0
39	262.5
40	309.0
41	279.5
42	250.0
43	250.0
44	245.5
45	241.0
46	307.5
47	374.0
48	374.0
49	325.5
50	277.0
51	233.0
52	189.0
53	189.0
54	169.5
55	150.0
56	133.5
57	117.0
58	117.0
59	89.5
60	62.0
61	41.0
62	20.0
63	20.0
64	21.0
65	22.0
66	11.5
67	1.0
68	1.0
69	2.0
70	3.0
71	2.0
72	1.0
73	1.0
74	0.5
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.09085402786190189
8	0.12113870381586916
9	0.09085402786190189
10	0.18170805572380377
11	0.0
12	0.0
13	0.18170805572380377
14	0.0
15	0.0
16	0.0
17	0.03028467595396729
18	0.0
19	0.03028467595396729
20	0.0
21	0.0
22	0.0
23	0.03028467595396729
24	0.0
25	0.18170805572380377
26	0.4239854633555421
27	0.5451241671714113
28	0.09085402786190189
29	0.6056935190793459
30	0.06056935190793458
31	0.03028467595396729
32	0.39370078740157477
33	0.03028467595396729
34	0.0
35	0.0
36	0.09085402786190189
37	0.0
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	3302.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.2198667474258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.9375	83.67655966081163
2	2.638888888888889	4.603270745003028
3	0.5208333333333333	1.362810417928528
4	0.3125	1.0902483343428226
5	0.1736111111111111	0.7571168988491823
6	0.06944444444444445	0.36341611144760755
7	0.0	0.0
8	0.10416666666666667	0.7268322228952151
9	0.034722222222222224	0.27256208358570566
>10	0.1388888888888889	2.877044215626893
>50	0.06944444444444445	4.270139309509388
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	81	2.453058752271351	No Hit
GAATTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT	60	1.8170805572380373	No Hit
GAATTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG	37	1.1205330102967896	No Hit
GAATTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23	0.6965475469412478	No Hit
GAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20	0.6056935190793459	No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	15	0.4542701393095093	No Hit
GAATTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9	0.27256208358570566	No Hit
GAATTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8	0.24227740763173833	No Hit
GAATTAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT	8	0.24227740763173833	No Hit
GAATTAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT	8	0.24227740763173833	No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	6	0.18170805572380377	No Hit
GAATTAGGGACTAATCCTAGCCCTAGCCCTACACAAATAT	6	0.18170805572380377	No Hit
GAATTAGGGGAGTGCAGTGCTGAATTAGGGGTAGAAGGTC	5	0.15142337976983647	No Hit
GAATTAGGGGGGCAATTTGTTTGGTGAAATGGCCCAGCTG	5	0.15142337976983647	No Hit
GAATTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	5	0.15142337976983647	No Hit
GAATTAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG	5	0.15142337976983647	No Hit
GAATTAGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT	5	0.15142337976983647	No Hit
GAATTAGGGTACCTGGTTGATCCTGCCAGTACCATATGCT	4	0.12113870381586916	No Hit
GAATTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC	4	0.12113870381586916	No Hit
GAATTAGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCT	4	0.12113870381586916	No Hit
GAATTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCG	4	0.12113870381586916	No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	4	0.12113870381586916	No Hit
GAATTAGGGCGACAGCAAAGCATCAAACCTGGGCCTGAGC	4	0.12113870381586916	No Hit
GAATTAGGGGCTGAATTAGGGTCACAGATCCTCTTCTGAG	4	0.12113870381586916	No Hit
GAATTAGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTA	4	0.12113870381586916	No Hit
GAATTAGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT	4	0.12113870381586916	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.12113870381586916	0.0	0.0	0.0	0.0
14	0.15142337976983647	0.0	0.0	0.0	0.0
15	0.15142337976983647	0.0	0.0	0.0	0.0
16	0.15142337976983647	0.0	0.0	0.0	0.0
17	0.15142337976983647	0.0	0.0	0.0	0.0
18	0.15142337976983647	0.0	0.0	0.0	0.0
19	0.15142337976983647	0.0	0.0	0.0	0.0
20	0.15142337976983647	0.0	0.0	0.0	0.0
21	0.15142337976983647	0.0	0.0	0.0	0.0
22	0.15142337976983647	0.0	0.0	0.0	0.0
23	0.15142337976983647	0.0	0.0	0.0	0.0
24	0.15142337976983647	0.0	0.0	0.0	0.0
25	0.18170805572380375	0.0	0.0	0.0	0.0
26	0.18170805572380375	0.0	0.0	0.0	0.0
27	0.18170805572380375	0.0	0.0	0.0	0.0
28	0.18170805572380375	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTGCCA	20	0.0021569119	34.734375	22
ATCCTGC	20	0.0023331598	34.2	20
CTCTTCT	25	1.5638264E-4	34.2	19
GATCCTG	20	0.0023331598	34.2	19
TCTTCTG	25	1.5638264E-4	34.2	20
TCCTGCC	20	0.0023331598	34.2	21
CTTCTGA	25	1.5638264E-4	34.2	21
GGGTCAC	25	1.7169006E-4	33.68182	7
GTCACAG	25	1.7169006E-4	33.68182	9
AGGGTCA	25	1.7169006E-4	33.68182	6
AGGGTAA	25	1.7169006E-4	33.68182	6
TTAGGGT	75	0.0	33.68182	4
TAGGGTC	25	1.7169006E-4	33.68182	5
TAGGGAT	35	7.732833E-7	33.68182	5
TAGGGAG	30	1.158253E-5	33.68182	5
TCACAGA	25	1.7169006E-4	33.68182	10
CACAGAT	25	1.7169006E-4	33.68182	11
ATCCTCT	20	0.0025206269	33.681816	16
TCCTCTT	20	0.0025206269	33.681816	17
GTTGATC	20	0.0025206269	33.681816	16
>>END_MODULE