FastQCFastQC Report
Fri 17 Jun 2016
SRR1525830_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525830_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77692
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG11091.4274313957679041No Hit
AGTCGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT4330.5573289399165937No Hit
AGTCGTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC3630.46722957318642844No Hit
AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG2890.37198167121453946No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2450.3153477835555784No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1960.25227822684446277No Hit
AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1420.18277300108119238No Hit
AGTCGTATGCCGTCTTCTGCTTGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG1380.17762446583946867Illumina Single End Adapter 2 (97% over 34bp)
AGTCGTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT1310.16861452916645214No Hit
AGTCGTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1280.16475312773515935No Hit
AGTCGTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT1190.15316892344128097No Hit
AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG1110.1428718529578335No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC900.11584204293878392No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC900.11584204293878392No Hit
AGTCGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA870.11198064150749112No Hit
AGTCGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT860.11069350769706018No Hit
AGTCGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG850.10940637388662926No Hit
AGTCGTGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTG820.10554497245533645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCA600.050.4536641
TGTTTAA204.016639E-450.3550538
TGCTCGT650.050.3222737
TTGTTTA204.0295834E-450.3222637
ACTATTA204.0686154E-450.2241747
ATGCTCG650.050.1915636
TACTATT204.0816923E-450.1915646
ATTATAC204.0816923E-450.1915642
TATACTA204.0816923E-450.1915644
TATGCTC650.050.15898535
CGATTAA150.00831792850.1264649
TATTATA301.0172153E-650.1264649
ATGGCCT252.0463913E-550.09397510
GACTTAA150.00833928950.09397510
ATCTTAG150.00833928950.09397548
CACACAT150.00833928950.09397511
ATATGCC301.0263648E-650.0615334
CAAATAT301.0263648E-650.0615334
ATTATAT355.1535608E-850.06152750
GGAGACT204.14758E-450.0291259
Produced by FastQC (version 0.11.5)