##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525830_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	77692
Sequences flagged as poor quality	0
Sequence length	56
%GC	55
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.43850074653761	38.0	36.0	39.0	33.0	40.0
2	35.36864799464552	38.0	36.0	39.0	27.0	40.0
3	35.503603974669204	38.0	36.0	39.0	28.0	40.0
4	35.53919322452762	38.0	36.0	39.0	28.0	40.0
5	34.990076198321574	38.0	35.0	39.0	25.0	40.0
6	34.881043093239974	38.0	35.0	39.0	26.0	40.0
7	35.98765638675797	38.0	38.0	39.0	31.0	40.0
8	35.77224167224425	38.0	36.0	39.0	30.0	40.0
9	35.80427843278587	38.0	36.0	39.0	30.0	40.0
10	34.63254903979818	38.0	35.0	39.0	26.0	40.0
11	33.59074293363538	37.0	32.0	39.0	24.0	40.0
12	32.958374092570665	37.0	31.0	39.0	19.0	40.0
13	32.54557740822736	37.0	31.0	39.0	16.0	40.0
14	33.31543788292231	38.0	32.0	39.0	20.0	40.0
15	32.27544663543222	37.0	31.0	39.0	14.0	40.0
16	32.10367862843021	36.0	30.0	39.0	17.0	40.0
17	31.044663543221954	35.0	27.0	38.0	13.0	40.0
18	30.432515574319105	35.0	26.0	38.0	12.0	40.0
19	31.58737064305205	36.0	29.0	38.0	16.0	40.0
20	32.23777222880091	36.0	30.0	39.0	18.0	40.0
21	32.41006796066519	37.0	30.0	39.0	17.0	40.0
22	31.721245430674973	36.0	29.0	39.0	14.0	40.0
23	32.40074911187767	37.0	30.0	39.0	17.0	40.0
24	31.730770220872163	36.0	29.0	39.0	14.0	40.0
25	32.204010708953305	36.0	30.0	39.0	17.0	40.0
26	32.6254440611646	37.0	31.0	39.0	16.0	40.0
27	32.76052875456932	38.0	32.0	39.0	17.0	40.0
28	32.70141069865623	37.0	31.0	39.0	18.0	40.0
29	32.32455079030016	37.0	31.0	39.0	15.0	40.0
30	32.03772589198373	36.0	30.0	39.0	14.0	40.0
31	32.725969211759256	37.0	33.0	39.0	15.0	40.0
32	32.535717963239456	37.0	32.0	39.0	14.0	40.0
33	32.319402255058435	37.0	31.0	39.0	11.0	40.0
34	32.08497657416465	36.0	31.0	39.0	10.0	40.0
35	31.946867116305413	36.0	31.0	39.0	9.0	40.0
36	31.263862431138342	36.0	30.0	38.0	4.0	40.0
37	30.98488904906554	36.0	30.0	38.0	4.0	40.0
38	30.487810842815218	35.0	29.0	38.0	4.0	40.0
39	29.49629305462596	35.0	27.0	38.0	4.0	39.0
40	27.405756062400247	33.0	18.0	38.0	4.0	39.0
41	25.54292591257787	31.0	13.0	37.0	4.0	39.0
42	26.342210266179272	31.0	17.0	37.0	4.0	39.0
43	26.33402409514493	32.0	17.0	37.0	4.0	39.0
44	25.42006899037224	31.0	13.0	37.0	4.0	39.0
45	26.302708129537148	32.0	18.0	37.0	4.0	39.0
46	24.340549863563815	29.0	10.0	36.0	4.0	39.0
47	23.304175462081037	28.0	6.0	35.0	4.0	39.0
48	24.027081295371467	29.0	4.0	35.0	4.0	39.0
49	22.96932760129743	27.0	4.0	35.0	4.0	39.0
50	23.549001184163107	28.0	4.0	35.0	4.0	39.0
51	23.729225660299644	29.0	4.0	36.0	4.0	39.0
52	23.54829326056737	29.0	4.0	36.0	4.0	39.0
53	23.364529166452144	29.0	4.0	36.0	4.0	39.0
54	23.005418833341913	29.0	4.0	35.0	4.0	38.0
55	22.649667919476908	29.0	4.0	35.0	4.0	38.0
56	21.80552695258199	27.0	4.0	35.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	3.5296389436099957
1	2	5.041041348899888
1	3	4.45763985395056
1	4	4.715032599721695
1	5	5.505042997524967
1	6	5.571332048954069
1	7	4.528710066279913
1	8	4.699011500497818
1	9	4.411858542408297
1	10	4.336172781703375
1	11	-1.6830204027008797
1	12	-7.319224754290975
1	13	-18.06951608180797
1	14	-6.2957075566359535
1	15	1.4282804271676852
1	16	0.7812408314173425
1	17	-3.9296184549693685
1	18	-2.381198727911702
1	19	2.0400100012974605
1	20	0.9406156647424453
1	21	6.848560211707305
1	22	7.323508979980527
1	23	7.201616071401592
1	24	7.662426738399063
1	25	5.790536953194277
1	26	7.2750278118044065
1	27	6.55975474338803
1	28	6.633988198545847
1	29	7.558661493056647
1	30	7.1353428280211055
1	31	6.019661118269326
1	32	5.730369986274251
1	33	5.55246513604337
1	34	5.723922724885007
1	35	6.080980695960019
1	36	5.595102310931306
1	37	5.59403858756464
1	38	6.484884748242006
1	39	6.084532614981434
1	40	-8.048330113145315
1	41	0.9589601152272351
1	42	0.6263653793755672
1	43	-7.434775867837697
1	44	-14.21700272730292
1	45	-0.9217680827110577
1	46	-3.2601732279192994
1	47	-9.314300109374445
1	48	-3.964554406556026
1	49	-12.232264542420715
1	50	-2.039887358575797
1	51	3.777798078503679
1	52	4.048544110543556
1	53	3.921302564244513
1	54	4.049303769232623
1	55	4.015572627431407
1	56	7.557534862743015
2	1	3.731112612612378
2	2	5.512543190986143
2	3	4.915777540868135
2	4	5.275821446070026
2	5	5.99084809757553
2	6	5.905074734788066
2	7	4.726282846746578
2	8	5.1248584989807995
2	9	4.8622666909310155
2	10	5.223263730197196
2	11	4.112110710497127
2	12	0.9769538197175507
2	13	1.8496488390906762
2	14	5.083545495448135
2	15	5.833250448116903
2	16	5.752995508871649
2	17	8.062940961342122
2	18	6.107279203172602
2	19	6.8931487717951825
2	20	5.793465480533818
2	21	7.905773243564564
2	22	8.663608669354222
2	23	7.668566876862837
2	24	8.857398926508573
2	25	7.388095285925623
2	26	6.723196561352914
2	27	6.732838612491545
2	28	6.588116638912819
2	29	7.425236657399637
2	30	7.512789913190506
2	31	6.005863531041122
2	32	5.395904913674382
2	33	5.699109649515879
2	34	5.671044013622996
2	35	5.810157897433694
2	36	7.042982105773465
2	37	7.322276686242674
2	38	7.6859972238614525
2	39	7.727167881903348
2	40	6.92660306607884
2	41	10.508624205381107
2	42	9.7118959724551
2	43	8.860680319404953
2	44	-1.9050038831217435
2	45	8.576462069712552
2	46	8.513358520979061
2	47	5.377746412333277
2	48	8.387681380484356
2	49	2.123438701095864
2	50	9.508476435399498
2	51	11.881388340730073
2	52	11.59047866230257
2	53	12.340792559187804
2	54	11.94087351567487
2	55	12.209894667451636
2	56	13.735386484501301
3	1	3.6656178924756517
3	2	5.268265586151863
3	3	4.604784438261774
3	4	4.704009201670345
3	5	5.747913348140145
3	6	5.476459707015373
3	7	4.706933521226219
3	8	4.606699431654917
3	9	4.7124772750731125
3	10	5.117056075921425
3	11	4.876683743519159
3	12	5.139988672919042
3	13	6.015186286444646
3	14	5.442148925833287
3	15	1.7248453940974926
3	16	6.870068084102073
3	17	7.871889261409265
3	18	8.42962553778775
3	19	6.343615719240709
3	20	6.386885489079262
3	21	7.368509006245393
3	22	7.857468157848391
3	23	7.52463719365463
3	24	8.287417848561923
3	25	6.42007965997189
3	26	6.065014299251956
3	27	6.181474393484034
3	28	5.8981941582926645
3	29	6.158618821838388
3	30	6.31911354134164
3	31	5.773732663678615
3	32	5.050119648460441
3	33	5.313221838980571
3	34	5.2190510941332775
3	35	5.905317514109516
3	36	7.054884687718168
3	37	7.2735066197102896
3	38	8.17516282234769
3	39	8.768552243741837
3	40	9.835533054481452
3	41	13.014148224181081
3	42	10.93267360780882
3	43	11.319082321480277
3	44	11.101893510518003
3	45	10.969491429414678
3	46	12.696231010755046
3	47	14.012917016007815
3	48	12.910845636115194
3	49	13.76837552585323
3	50	11.688296981087102
3	51	12.059072525555809
3	52	11.691314894982785
3	53	12.378453549238465
3	54	11.983234499621641
3	55	12.058457201786005
3	56	11.150878152693942
4	1	3.5438932464381097
4	2	5.145067431241237
4	3	4.893912505402525
4	4	4.988973004604553
4	5	5.602548494530048
4	6	5.895769079813114
4	7	4.821226557295155
4	8	4.881284606874338
4	9	4.853741930079842
4	10	5.0009692029635175
4	11	5.85682340653409
4	12	6.5298919338582415
4	13	6.617723284330481
4	14	6.035780804877433
4	15	7.312198844030224
4	16	7.609961735200798
4	17	8.94671788592077
4	18	9.605229356097702
4	19	8.245787543188428
4	20	7.26740313915294
4	21	7.326610101767205
4	22	8.589033280534021
4	23	7.839647764479157
4	24	8.035768159280103
4	25	7.3932361722404565
4	26	6.679531564932013
4	27	6.5128216931188625
4	28	5.712083581061581
4	29	6.980452379401328
4	30	6.677688722326966
4	31	5.723505230787332
4	32	6.21259001811897
4	33	5.825874795608161
4	34	5.678657731329771
4	35	5.770267222611022
4	36	6.327227566826377
4	37	6.965863593251193
4	38	7.584285764240189
4	39	7.181583187428174
4	40	0.9038269874688325
4	41	11.709964740293582
4	42	10.121474940373371
4	43	10.220997883015283
4	44	11.147316023170958
4	45	9.6417318035578
4	46	12.498716756158341
4	47	12.701622250167599
4	48	12.226817190987386
4	49	12.110293650012373
4	50	12.123430557869728
4	51	12.106481071907272
4	52	11.606812364391462
4	53	11.28645534307536
4	54	12.301256153795517
4	55	11.256227718795422
4	56	11.04286354573642
5	1	3.521628536927807
5	2	5.256637679175121
5	3	4.72985675111611
5	4	5.058110786768111
5	5	5.724541833619718
5	6	5.431047234494258
5	7	4.582728962623882
5	8	4.778225522132757
5	9	4.663535935260548
5	10	5.4176461488127785
5	11	6.337519101612415
5	12	6.410612298974808
5	13	6.668605238197507
5	14	6.5791383970816995
5	15	7.475452822073887
5	16	7.886266163472662
5	17	9.307270494777313
5	18	9.95831927838708
5	19	7.9408041954642385
5	20	7.546667972013431
5	21	7.550737399164515
5	22	8.652620304093912
5	23	8.05674410097848
5	24	7.85481342880027
5	25	7.719744128327783
5	26	6.554022745393347
5	27	6.102682307402812
5	28	6.744833056823264
5	29	7.079132532355565
5	30	7.199768406550479
5	31	5.742377810039827
5	32	5.936038889464722
5	33	6.212330944615186
5	34	6.047351452805039
5	35	6.418102426755585
5	36	7.482340432251316
5	37	7.8407864993817
5	38	8.087924594880374
5	39	9.105414444366616
5	40	9.791393288196595
5	41	12.197012970218335
5	42	11.586381810979017
5	43	11.2858184084871
5	44	12.681669051418154
5	45	11.605960248111895
5	46	12.853410230933225
5	47	13.741772737400854
5	48	12.967596764195722
5	49	14.21440121137909
5	50	12.636752378518548
5	51	11.767391396318278
5	52	11.348147013460167
5	53	11.419180148798809
5	54	12.397037577256722
5	55	11.845163206645427
5	56	11.556987142627992
6	1	3.365036497126816
6	2	5.056637679175118
6	3	4.640055756091229
6	4	4.8605983489571685
6	5	5.421556758992857
6	6	5.434405443449478
6	7	4.5365846840169155
6	8	4.761931989794448
6	9	4.767516034763041
6	10	5.156203362743128
6	11	5.677071340418387
6	12	6.709368517880282
6	13	7.460147526754717
6	14	6.238466755290652
6	15	7.299457797198269
6	16	7.412509944567191
6	17	8.865106315672833
6	18	9.040284452516431
6	19	8.46493354869807
6	20	6.360722698381586
6	21	7.9855632698112835
6	22	8.417545677228237
6	23	7.293435643267035
6	24	7.7436193989495195
6	25	7.559420745243209
6	26	6.062107322507771
6	27	6.28651315317396
6	28	5.904783305579489
6	29	6.561719597032187
6	30	6.828997262271866
6	31	5.7750892528259
6	32	5.7844219740418374
6	33	6.084345869988322
6	34	6.2008340398697115
6	35	5.872953173024239
6	36	7.3356986412065375
6	37	7.568274061570754
6	38	8.070511659556995
6	39	8.688125887152687
6	40	4.9525873180473425
6	41	12.764923417979528
6	42	11.435386786103397
6	43	11.15260945326322
6	44	12.731544673308704
6	45	11.635189103833287
6	46	13.783634111530244
6	47	14.779583682674481
6	48	12.68177586867333
6	49	13.92186389794626
6	50	12.698568298916559
6	51	12.233684928656583
6	52	12.777749003509914
6	53	12.186593084122187
6	54	11.673156980241796
6	55	12.65884479868523
6	56	11.426265749593167
7	1	3.4084125655028856
7	2	5.218176140713581
7	3	4.647320713356187
7	4	4.949487237846057
7	5	5.287368724804821
7	6	5.498721682765719
7	7	4.388507760939994
7	8	4.177957630820089
7	9	4.209396376643383
7	10	5.048083704623473
7	11	5.29031920366625
7	12	6.103812962324728
7	13	6.6379253045325015
7	14	5.662184704008602
7	15	7.31975694249741
7	16	7.440928747985993
7	17	8.329849905416424
7	18	9.11656650379848
7	19	7.324335258099779
7	20	6.50495346761236
7	21	7.354794039042051
7	22	7.95387046355302
7	23	6.248350173181567
7	24	7.9483202536503725
7	25	7.151942112764573
7	26	6.018303903704357
7	27	4.9689917856525945
7	28	5.8445268953230745
7	29	6.2198392551518396
7	30	5.546304954579565
7	31	5.097525150261795
7	32	5.344892059511917
7	33	4.817892878535336
7	34	5.555962244997922
7	35	4.892397617468685
7	36	6.447450777958675
7	37	6.291992010288702
7	38	6.655981745027081
7	39	7.38791221193901
7	40	7.497031762491787
7	41	9.251675554731666
7	42	9.734104734821347
7	43	9.786583812237577
7	44	9.783467750231779
7	45	8.5721549157991
7	46	11.171881974778106
7	47	11.54283154592235
7	48	10.554852791750257
7	49	11.610752786835146
7	50	10.901773427121686
7	51	8.833471253442909
7	52	10.333731909492823
7	53	9.82548197301108
7	54	8.861191168275983
7	55	9.053289243129672
7	56	8.367932416259833
8	1	3.7377956007682727
8	2	5.403696502704534
8	3	4.70126023788395
8	4	5.09141067388714
8	5	5.691304658152518
8	6	5.856044098911667
8	7	4.902621098582742
8	8	4.548136471587171
8	9	4.839504830281243
8	10	5.238416247897192
8	11	6.200180584115863
8	12	7.110348910037143
8	13	7.232696546362561
8	14	7.032794486383089
8	15	7.9167547159657765
8	16	8.01005896417503
8	17	9.437585307269472
8	18	10.297777449715312
8	19	8.578518982871742
8	20	7.421997208185509
8	21	7.865675314629211
8	22	8.604520467144201
8	23	7.7968670158160585
8	24	8.345720239285654
8	25	7.988902537960293
8	26	6.8880877146646355
8	27	6.854720436087124
8	28	6.31920907588761
8	29	7.4888904653795265
8	30	7.663521071795682
8	31	6.214094855066797
8	32	6.4751082485516385
8	33	6.700102172509329
8	34	5.6645595300657945
8	35	6.447953173024242
8	36	7.604816288265365
8	37	7.5841704201141695
8	38	8.169951435467357
8	39	8.488896195275942
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115	35	5.149251874322946
115	36	6.284724615232516
115	37	6.50777622607292
115	38	7.367048456093787
115	39	8.201654025680824
115	40	9.286353551813576
115	41	8.803235106291218
115	42	7.413092413809025
115	43	6.895141920795687
115	44	-2.336745369981344
115	45	7.621660965305146
115	46	2.5424003452964747
115	47	-7.0563470532562516
115	48	1.4889187258161911
115	49	-3.7586555825732226
115	50	2.652356177704437
115	51	2.906953326924981
115	52	2.639870215631131
115	53	3.2049913525204587
115	54	3.16666347374829
115	55	4.136983326823756
115	56	6.229404277731696
116	1	3.44626360335392
116	2	4.383011305548749
116	3	3.7633158293513063
116	4	4.338986627345442
116	5	5.057820495256593
116	6	4.567097751141787
116	7	4.209020581452819
116	8	4.104697557560016
116	9	4.134915302162305
116	10	4.438804095343862
116	11	4.163335076682124
116	12	3.4542403127520735
116	13	5.208132874740066
116	14	4.361818403642303
116	15	-2.3237107009702314
116	16	4.666813973871221
116	17	6.322523898090417
116	18	6.244771632003612
116	19	5.783431717196237
116	20	5.113012075670966
116	21	6.439043123291139
116	22	7.5521610618436235
116	23	6.462025386856784
116	24	7.544168849498973
116	25	6.495043455865918
116	26	5.638572523972975
116	27	5.9555607722215775
116	28	5.3573474081435855
116	29	5.876737912050501
116	30	5.64154304981766
116	31	4.84111489385154
116	32	4.724623439243299
116	33	4.963192023834477
116	34	5.116401805437484
116	35	5.162238887309957
116	36	5.651357981865882
116	37	6.676607394904089
116	38	6.270145359190696
116	39	6.9568987809255844
116	40	9.3053345707946
116	41	7.576461879517989
116	42	5.6938116945283035
116	43	0.7353017609555259
116	44	1.2266911934552205
116	45	4.52575686940105
116	46	-0.812245009348878
116	47	-5.236167233076431
116	48	-0.09349885660139279
116	49	-3.4649492888669293
116	50	0.7332752586235181
116	51	4.5233369433086
116	52	4.259250835011748
116	53	4.577618725147829
116	54	4.266563573648391
116	55	4.469650659491091
116	56	7.045588093915512
117	1	2.3649121190173616
117	2	3.3238018582796016
117	3	3.0134388406683428
117	4	3.423782428559157
117	5	4.351407505261513
117	6	3.647465144942018
117	7	3.1125797088925395
117	8	3.367777760938729
117	9	3.588909069588908
117	10	3.4325715219471107
117	11	-1.5729286595816134
117	12	-10.514760835353549
117	13	-9.32393207523533
117	14	-3.6447421999332263
117	15	-7.517084491358947
117	16	-7.815226373840769
117	17	0.0684645246280553
117	18	-4.4670538559412805
117	19	-0.6188972970730724
117	20	-0.9010932220164207
117	21	4.610438891701829
117	22	5.137694930959579
117	23	4.444803802471018
117	24	5.004067160143549
117	25	5.018251591014355
117	26	5.337604834945587
117	27	5.460891262626699
117	28	4.797071862793413
117	29	5.5865952189227315
117	30	6.043051988640027
117	31	4.458795720487593
117	32	3.9658896357333795
117	33	3.6600921386450374
117	34	4.405560408028919
117	35	5.2539233222779735
117	36	5.609206103893104
117	37	4.064667096396626
117	38	5.446133550104253
117	39	6.4113845936203475
117	40	7.515273885211521
117	41	6.741789089621321
117	42	5.526680318441706
117	43	-2.6171666661397666
117	44	0.11450487231367745
117	45	4.919393083932789
117	46	-0.2883808138428918
117	47	-0.2507645760319832
117	48	0.40789527165840767
117	49	-1.4423152065313545
117	50	2.509886706876756
117	51	5.3047048291540975
117	52	4.810833580624344
117	53	4.777389104022685
117	54	5.404500263823884
117	55	5.41979007231707
117	56	7.69131550083695
118	1	2.940036497126812
118	2	4.339971012508457
118	3	3.8067224227579004
118	4	4.027265015623833
118	5	5.021556758992858
118	6	4.626072110116148
118	7	3.7282513506835855
118	8	3.4869319897944493
118	9	3.317516034763038
118	10	3.9728700294097976
118	11	-0.3062619929149477
118	12	-4.673964815453054
118	13	-3.9898524732452785
118	14	-9.536533244709346
118	15	-8.525542202801734
118	16	-8.529156722099476
118	17	0.6734396490061663
118	18	-4.8180488808168995
118	19	-0.18506645130192823
118	20	-0.09761063495174582
118	21	5.6688966031446135
118	22	7.334212343894901
118	23	6.351768976600372
118	24	7.393619398949518
118	25	6.651087411909877
118	26	6.303773989174438
118	27	5.369846486507296
118	28	5.504783305579483
118	29	5.645052930365516
118	30	5.870663928938537
118	31	4.800089252825899
118	32	5.176088640708503
118	33	5.092679203321655
118	34	5.150834039869714
118	35	4.9812865063575735
118	36	6.2106986412065375
118	37	6.559940728237422
118	38	6.98717832622366
118	39	6.863125887152691
118	40	8.469253984714008
118	41	6.4482567513128615
118	42	5.785386786103395
118	43	1.1109427865965529
118	44	-1.0101219933579664
118	45	5.676855770499952
118	46	0.09196744486357744
118	47	0.8962503493411518
118	48	0.9817758686733349
118	49	-0.5614694353870782
118	50	2.0735682989165554
118	51	6.442018261989919
118	52	5.911082336843251
118	53	6.003259750788857
118	54	6.889823646908461
118	55	6.642178132018561
118	56	7.951265749593169
119	1	2.741078163793482
119	2	4.100387679175121
119	3	3.3723474227578976
119	4	4.0033066822905
119	5	4.727806758992855
119	6	4.087530443449481
119	7	3.3063763506835855
119	8	3.437973656461118
119	9	3.482099368096371
119	10	3.404120029409796
119	11	-1.0573036595816134
119	12	-7.397923148786386
119	13	-4.285685806578613
119	14	-6.9584082447093465
119	15	-9.221375536135067
119	16	-8.805198388766144
119	17	0.1463563156728327
119	18	-7.1013822141502345
119	19	-1.1611081179685954
119	20	-1.509068968285078
119	21	3.9553549364779492
119	22	5.105045677228237
119	23	4.666352309933703
119	24	4.171744398949521
119	25	4.5604624119098744
119	26	6.2860656558411065
119	27	5.732346486507293
119	28	5.400616638912819
119	29	6.384636263698852
119	30	6.005038928938536
119	31	4.970922586159233
119	32	4.5927553073751675
119	33	4.668720869988324
119	34	4.7987507065363815
119	35	5.026078173024242
119	36	5.966948641206539
119	37	6.582857394904089
119	38	7.22155332622366
119	39	7.6308342204860224
119	40	9.740087318047344
119	41	5.021173417979529
119	42	4.373928452770063
119	43	-3.6505155467367807
119	44	-2.5226219933579657
119	45	3.5518557704999516
119	46	-3.7538658884697576
119	47	-4.091249650658849
119	48	-5.4307241313266665
119	49	-4.106261102053743
119	50	-1.6274733677501096
119	51	2.6545182619899172
119	52	2.719415670176584
119	53	2.6293014174555225
119	54	2.391906980241796
119	55	2.6296781320185616
119	56	5.628349082926501
120	1	2.4352745923649124
120	2	4.199494822032264
120	3	3.7281509941864712
120	4	3.808217396576211
120	5	4.435842473278569
120	6	4.116548300592335
120	7	3.549679922112155
120	8	3.5750272278896915
120	9	3.4722779395249432
120	10	3.7014414579812254
120	11	-1.9443572310101835
120	12	-7.464441005929242
120	13	-7.430328663721468
120	14	-9.186533244709345
120	15	-1.6874469647064956
120	16	-8.767251960194717
120	17	0.940106315672832
120	18	-5.787096499864521
120	19	-0.023161689397166896
120	20	-0.5166582539993634
120	21	4.052229936477946
120	22	5.324688534371095
120	23	4.713673738505133
120	24	5.093619398949521
120	25	3.90823026905273
120	26	6.341869227269676
120	27	5.355560772221576
120	28	5.859545210341391
120	29	6.299814835127421
120	30	6.13494964322425
120	31	5.147708300444947
120	32	5.549898164518023
120	33	5.237917298559754
120	34	5.0175007065363815
120	35	5.233667458738527
120	36	6.867841498349396
120	37	6.205178823475521
120	38	6.380035469080806
120	39	6.825030649057453
120	40	7.566873032333056
120	41	-2.199362296306184
120	42	-1.0479465472299374
120	43	-5.3009619753082085
120	44	-14.188693421929393
120	45	-2.7017156580714783
120	46	-7.991365888469758
120	47	-4.996606793515992
120	48	-4.282509845612381
120	49	-13.66385038776803
120	50	-3.721669796321539
120	51	1.013446833418488
120	52	0.8372728130337279
120	53	1.798497846026951
120	54	1.1731569802417958
120	55	2.0707495605899915
120	56	4.348884797212214
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	3464.0
5	44.0
6	42.0
7	313.0
8	408.0
9	367.0
10	272.0
11	253.0
12	244.0
13	198.0
14	245.0
15	280.0
16	409.0
17	497.0
18	636.0
19	779.0
20	914.0
21	1153.0
22	1395.0
23	1684.0
24	1997.0
25	2196.0
26	2306.0
27	2513.0
28	2585.0
29	2867.0
30	3190.0
31	3645.0
32	4080.0
33	4674.0
34	5074.0
35	5565.0
36	5810.0
37	6528.0
38	6216.0
39	4849.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	98.49534057560624	1.4119857900427328	0.060495289090253826	0.03217834526077331
8	98.5313803222983	0.2934665087782526	1.1313906193687895	0.043762549554651704
9	98.60471103102071	0.5908096280087528	0.45436993178015184	0.350109409190372
10	60.735983112804405	18.02759614889564	12.72717911754106	8.509241620758894
11	35.813211141430266	26.773670390773823	15.90125109406374	21.511867373732173
12	29.947742367296502	19.18472944447305	20.81552798228904	30.05200020594141
13	23.077022087216186	15.84719147402564	24.976831591412243	36.09895484734593
14	21.746125727230602	22.896823353755856	29.081501312876483	26.275549606137055
15	29.60798510550269	17.965194455937112	25.678785684733143	26.74803475382706
16	30.557737627651214	22.883855098965913	22.850372812383295	23.708034460999574
17	27.106642118680075	19.37660130550656	30.159261500431306	23.357495075382058
18	25.07980229624672	18.851361787571435	26.892086701333472	29.176749214848375
19	30.159792951598575	20.451180098631266	20.842614887398117	28.546412062372045
20	26.127252571605354	23.95395601845884	25.801129186109463	24.117662223826343
21	26.51238222725635	19.00967924625444	26.924265046594243	27.553673479894968
22	24.83138547083355	18.770272357514287	28.779024867425218	27.619317304226946
23	25.33336765690161	20.316120063841836	26.32703495855429	28.023477320702263
24	24.641841187298073	20.79187529766119	25.469500186641607	29.096783328399127
25	27.798517069151952	22.19247206631996	25.70156016682972	24.307450697698368
26	29.678466114680845	21.40254188181666	21.87512072007108	27.043871283431415
27	24.793910041733216	23.7673244371168	20.826678345097633	30.61208717605235
28	27.093577036941692	26.129488994722617	21.069635731754406	25.707298236581284
29	32.73266305748904	23.297241557102346	19.70739881412735	24.262696571281257
30	27.181177803819555	21.80211968629673	28.045278353701725	22.97142415618199
31	29.382989405388706	27.031062936882893	20.640825941993537	22.945121715734864
32	34.36373467916366	22.80615923370069	20.381862189720877	22.44824389741477
33	30.956676374320253	23.026468389989947	20.82162830854875	25.19522692714105
34	26.12872857640277	24.150471351379235	27.01597823143288	22.704821840785115
35	24.5801054409054	24.221761043581385	30.45153972080073	20.74659379471249
36	27.431463191645527	26.260961382454063	24.464646724783993	21.842928701116417
37	27.94132810888843	22.107651062074655	26.269591668385235	23.681429160651682
38	32.39816319686299	23.169001367283233	22.917472847818797	21.515362588034982
39	28.29962488882014	22.63299689340913	21.123528880982764	27.943849336787967
40	23.254999156534264	18.27595603597057	24.31257542530138	34.15646938219379
41	20.451116083042823	16.34190124356106	22.28914095426401	40.917841719132106
42	26.988654697523135	23.874182015307767	22.841617512293972	26.295545774875123
43	20.91465303593556	19.712928541924825	28.287639405204462	31.084779016935148
44	22.002611202316473	21.17142155405318	25.331247818611924	31.49471942501842
45	26.180002837940684	21.020110679686795	28.323938029695178	24.47594845267734
46	22.63178960143571	24.280531419054135	24.066207893819477	29.021471085690674
47	20.034142493177967	23.55185391151404	23.44839181097474	32.965611784333255
48	25.224122829615126	24.611005203771448	25.689113297954556	24.47575866865887
49	21.904798771597054	24.02869714447928	24.606769119601545	29.45973496432212
50	25.303053750128957	23.568554627050446	24.929072526565562	26.19931909625503
51	22.902754995285637	26.439171822326703	26.344884595016982	24.31318858737068
52	24.143800288838456	24.83752836806272	27.469310913967405	23.549360429131422
53	22.764878225067438	24.339498444739867	27.928858142206277	24.96676518798642
54	23.12565316681074	24.600358677280763	28.061981498445306	24.2120066574632
55	23.471087183856852	23.40137108331074	27.35711426985295	25.770427462979463
56	25.840086718629017	21.083466680431528	27.65059619057451	25.42585041036494
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	2.5
9	3.0
10	15.5
11	28.0
12	62.5
13	115.0
14	133.0
15	126.5
16	120.0
17	114.0
18	108.0
19	128.5
20	149.0
21	129.5
22	129.0
23	148.0
24	159.5
25	171.0
26	190.0
27	209.0
28	242.5
29	304.0
30	332.0
31	398.0
32	464.0
33	515.0
34	566.0
35	712.5
36	859.0
37	931.5
38	1076.0
39	1148.0
40	1179.0
41	1210.0
42	1513.0
43	1816.0
44	2299.5
45	2783.0
46	3834.0
47	4886.0
48	4887.0
49	4349.5
50	3812.0
51	3930.0
52	4048.0
53	4377.5
54	4694.0
55	4681.0
56	4683.5
57	4686.0
58	4918.0
59	5150.0
60	5543.0
61	5936.0
62	5873.0
63	5251.0
64	4692.0
65	4273.0
66	3854.0
67	3364.5
68	2875.0
69	2462.0
70	2049.0
71	1753.5
72	1258.5
73	1059.0
74	852.5
75	646.0
76	502.0
77	358.0
78	274.5
79	154.0
80	117.0
81	76.0
82	35.0
83	26.0
84	17.0
85	9.5
86	2.0
87	1.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.002574267620861865
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.4479225660299645
16	0.050198218606806365
17	0.02702981001904958
18	0.0
19	0.037326880502497044
20	0.1467332543891263
21	0.0
22	0.0
23	0.0
24	0.003861401431292797
25	0.01029707048344746
26	0.04247541574422077
27	0.07207949338413222
28	0.002574267620861865
29	0.14415898676826444
30	0.050198218606806365
31	0.014158471914740256
32	0.025742676208618646
33	0.11584204293878392
34	0.19178293775420893
35	0.14544612057869535
36	0.04247541574422077
37	0.1390104515265407
38	0.21366421253153478
39	0.14802038819955723
40	0.8096071667610566
41	1.050301189311641
42	0.2767337692426505
43	0.2831694382948051
44	0.4286155588735005
45	0.22009988158368946
46	0.3076249806929928
47	0.4749523760490141
48	0.07207949338413222
49	0.24841682541316992
50	0.190495803943778
51	0.3462389950059208
52	0.18019873346033052
53	0.2741595016217886
54	0.2381197549297225
55	0.3024764454512691
56	0.2574267620861865
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	77692.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.760490140555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.72369385404669	65.44303145755033
2	6.845705510766761	10.098851876641096
3	1.9020695913167907	4.208927560109149
4	0.8393536453425471	2.4764454512691136
5	0.4502146372107632	1.660402615455903
6	0.2826929117369909	1.2510940637388663
7	0.1797368512895683	0.9280234773207022
8	0.13087634802638468	0.7722802862585594
9	0.10993613234216311	0.7298048705143387
>10	0.4781349247897253	6.214282036760542
>50	0.03664537744738771	1.8354528136745096
>100	0.01919519771053642	2.95397209493899
>500	0.0	0.0
>1k	0.0017450179736851288	1.4274313957679041
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	1109	1.4274313957679041	No Hit
AGTCGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	433	0.5573289399165937	No Hit
AGTCGTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	363	0.46722957318642844	No Hit
AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	289	0.37198167121453946	No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	245	0.3153477835555784	No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	196	0.25227822684446277	No Hit
AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	142	0.18277300108119238	No Hit
AGTCGTATGCCGTCTTCTGCTTGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG	138	0.17762446583946867	Illumina Single End Adapter 2 (97% over 34bp)
AGTCGTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	131	0.16861452916645214	No Hit
AGTCGTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	128	0.16475312773515935	No Hit
AGTCGTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	119	0.15316892344128097	No Hit
AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	111	0.1428718529578335	No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	90	0.11584204293878392	No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	90	0.11584204293878392	No Hit
AGTCGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	87	0.11198064150749112	No Hit
AGTCGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	86	0.11069350769706018	No Hit
AGTCGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG	85	0.10940637388662926	No Hit
AGTCGTGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGCACTG	82	0.10554497245533645	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0012871338104309324	0.0	0.0	0.0	0.0
11	0.002574267620861865	0.0	0.0	0.0	0.0
12	0.003861401431292797	0.0	0.0	0.0	0.0
13	0.003861401431292797	0.0	0.0	0.0	0.0
14	0.009009936673016528	0.0	0.0	0.0	0.0
15	0.01029707048344746	0.0	0.0	0.0	0.0
16	0.01029707048344746	0.0	0.0	0.0	0.0
17	0.01029707048344746	0.0	0.0	0.0	0.0
18	0.011584204293878391	0.0	0.0	0.0	0.0
19	0.012871338104309325	0.0	0.0	0.0	0.0
20	0.012871338104309325	0.0	0.0	0.0	0.0
21	0.01673273953560212	0.0	0.0	0.0	0.0
22	0.019307007156463985	0.0	0.0	0.0	0.0
23	0.023168408587756782	0.0	0.0	0.0	0.0
24	0.029604077639911446	0.0	0.0	0.0	0.0
25	0.030891211450342376	0.0	0.0	0.0	0.0
26	0.03475261288163518	0.0	0.0	0.0	0.0
27	0.039901148123358904	0.0	0.0	0.0	0.0
28	0.04118828193378984	0.0	0.0	0.0	0.0
29	0.04504968336508263	0.0	0.0	0.0	0.0
30	0.04504968336508263	0.0	0.0	0.0	0.0
31	0.04891108479637543	0.0	0.0	0.0	0.0
32	0.052772486227668225	0.0	0.0	0.0	0.0
33	0.05405962003809916	0.0	0.0	0.0	0.0
34	0.05534675384853009	0.0	0.0	0.0	0.0
35	0.05792102146939196	0.0	0.0	0.0	0.0
36	0.05920815527982289	0.0	0.0	0.0	0.0
37	0.060495289090253826	0.0	0.0	0.0	0.0
38	0.060495289090253826	0.0	0.0	0.0	0.0
39	0.06178242290068475	0.0	0.0	0.0	0.0
40	0.06306955671111569	0.0	0.0	0.0	0.0
41	0.06306955671111569	0.0	0.0	0.0	0.0
42	0.06435669052154662	0.0	0.0	0.0	0.0
43	0.06693095814240849	0.0	0.0	0.0	0.0
44	0.07465376100499407	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTCA	60	0.0	50.45366	41
TGTTTAA	20	4.016639E-4	50.35505	38
TGCTCGT	65	0.0	50.32227	37
TTGTTTA	20	4.0295834E-4	50.32226	37
ACTATTA	20	4.0686154E-4	50.22417	47
ATGCTCG	65	0.0	50.19156	36
TACTATT	20	4.0816923E-4	50.19156	46
ATTATAC	20	4.0816923E-4	50.19156	42
TATACTA	20	4.0816923E-4	50.19156	44
TATGCTC	65	0.0	50.158985	35
CGATTAA	15	0.008317928	50.12646	49
TATTATA	30	1.0172153E-6	50.12646	49
ATGGCCT	25	2.0463913E-5	50.093975	10
GACTTAA	15	0.008339289	50.093975	10
ATCTTAG	15	0.008339289	50.093975	48
CACACAT	15	0.008339289	50.093975	11
ATATGCC	30	1.0263648E-6	50.06153	34
CAAATAT	30	1.0263648E-6	50.06153	34
ATTATAT	35	5.1535608E-8	50.061527	50
GGAGACT	20	4.14758E-4	50.029125	9
>>END_MODULE