FastQCFastQC Report
Fri 17 Jun 2016
SRR1525829_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525829_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3635
Sequences flagged as poor quality0
Sequence length40
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT90.24759284731774417No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC90.24759284731774417No Hit
AGTCGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT80.2200825309491059No Hit
AGTCGTGGGATCCAGCGGTGTGTTGGGTGCGTTGATTTAG80.2200825309491059No Hit
AGTCGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT70.19257221458046767No Hit
AGTCGTGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT70.19257221458046767No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG50.1375515818431912No Hit
AGTCGTGGGCTCGTCTTAATCAGCAACATCTTTTGATACA50.1375515818431912No Hit
AGTCGTGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.1375515818431912No Hit
AGTCGTGGGAGAGTCGATTTTTTTTTTTTTTTTTTTTTTT40.11004126547455295No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC40.11004126547455295No Hit
AGTCGTGGGATTCATGGTGGGGAAAAAGTTTCCACTTTGA40.11004126547455295No Hit
AGTCGTGGGGTGCAGTGCTAGTCGGTGGGGGGGTCTCTTC40.11004126547455295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGG3600.033.902782
TCGTGGG3500.033.902783
CGTGGGT405.0509698E-833.902784
CGTGGGG1200.033.902784
CGTGGGA1400.033.902784
TGGGGGG405.0509698E-833.902786
GTGGGGT357.618182E-733.902785
GTGGGGC301.1376978E-533.902785
GGGGGCT200.00247193133.902789
GTGGGAT405.0509698E-833.902785
GTGGGAA600.033.902785
AGTCGTG3600.033.902781
GGGGCTG200.00247193133.9027810
TGGGAAG453.3123797E-933.902786
GGGCTGG200.00247193133.9027811
CGTGGGC502.1464075E-1033.9027754
GTGGGGG502.1464075E-1033.9027755
GGGAAGC251.68389E-433.9027757
TGGGATC251.68389E-433.9027756
GGGGGGC304.9129623E-428.2523148
Produced by FastQC (version 0.11.5)