FastQCFastQC Report
Fri 17 Jun 2016
SRR1525827_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525827_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4959
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT751.5124016938898972No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC591.189755999193386No Hit
AGTCGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT330.6654567453115547No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG180.3629764065335753No Hit
AGTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.34281105061504336No Hit
AGTCGTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG160.3226456946965114No Hit
AGTCGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA140.28231498285944745No Hit
AGTCGTGGGACTAATCCTAGCCCTAGCCCTACACAAATAT130.26214962694091554No Hit
AGTCGTGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG110.22181891510385157No Hit
AGTCGTGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT100.2016535591853196No Hit
AGTCGTGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT100.2016535591853196No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG80.1613228473482557No Hit
AGTCGTGGGAATTAAGGGTCTGAGTAGCCTTTGGGTGATA80.1613228473482557No Hit
AGTCGTATGCCGTCTTCTGCTTGGATCGGAAGAGCTCGTA80.1613228473482557Illumina Single End Adapter 1 (100% over 21bp)
AGTCGTGGGATGCCATCTACCTTCTTCAACCTCACCATAG70.14115749142972372No Hit
AGTCGTGGGATGCCACAACTAGATACATCAACATGATTTA60.12099213551119178No Hit
AGTCGTGGATTTGATCTTATCTTGGATCCTTTTGAAAAGT60.12099213551119178No Hit
AGTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12099213551119178No Hit
AGTCGTGGGTGGCTTTGGCCCTAAGCCATTGTCTCTCTGG50.1008267795926598No Hit
AGTCGTGGGTCACAGATCCTCTTCTGAGATGAGGATCGGA50.1008267795926598No Hit
AGTCGTGGGTACCTGGTTAATCCTGCCAGTAGCATATGCT50.1008267795926598No Hit
AGTCGTGGGCACGTCCAGAGTTCTTTGTCATTACCTTTAT50.1008267795926598No Hit
AGTCGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGC50.1008267795926598No Hit
AGTCGTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTG50.1008267795926598No Hit
AGTCGTGGGTCATAGATCCTCTTCTGAGATGAGTTTTTGT50.1008267795926598No Hit
AGTCGTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA50.1008267795926598No Hit
AGTCGTGGGTACCTGGTTGATACTGCCAGTAGCATATGCT50.1008267795926598No Hit
AGTCGTGGGTGCTTCATTTGGGGGTGGGGAAACATGTAAA50.1008267795926598No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC50.1008267795926598No Hit
AGTCGTGGGGGAGCCCCCAAACTGTGAATTCAGTTGCAAA50.1008267795926598No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCAC301.2083472E-534.0000047
GGTCACA251.7522005E-434.0000048
CGTGGGC502.582965E-1034.0000044
TGGGGGA251.7522005E-434.0000046
GTGGGTC502.582965E-1034.0000045
GTGGGAG251.7522005E-434.0000045
ATCCTCT405.6610588E-834.016
TCCTCTT405.6610588E-834.017
CTGAGAT358.293737E-734.024
GTCACAG200.002530474734.09
ACAGATC200.002530474734.012
CTCTTCT358.293737E-734.019
TTCTGAG405.6610588E-834.022
GAGTTTT453.8398866E-934.031
GATGAGT405.6610588E-834.028
CGTGGGT700.034.04
CCTCTTC358.293737E-734.018
TGGGGGG551.6370905E-1134.06
TGGGGGC200.002530474734.06
GTGGGGT358.293737E-734.05
Produced by FastQC (version 0.11.5)