FastQCFastQC Report
Fri 17 Jun 2016
SRR1525826_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525826_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117888
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG15191.2885111292073834No Hit
AGGGTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT5610.4758754071661238No Hit
AGGGTCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGC5050.4283726927252986No Hit
AGGGTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC4370.37069082519001084No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3670.31131243213897936No Hit
AGGGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG3410.28925760043431054No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2740.2324239956568947No Hit
AGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGT2460.2086726384364821No Hit
AGGGTCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT2120.17983170466883822No Hit
AGGGTCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT1890.16032166123778502No Hit
AGGGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1840.15608034744842564No Hit
AGGGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG1790.15183903365906623No Hit
AGGGTCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1710.1450529315960912No Hit
AGGGTCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC1610.1365703040173724No Hit
AGGGTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1490.12639115092290987No Hit
AGGGTCGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCG1460.12384636264929423No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1410.11960504885993486No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1200.10179153094462541No Hit
AGGGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1190.10094326818675353No Hit
AGGGTCGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT1180.10009500542888165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTATA150.008002683550.6349941
ACCCAAC150.008002683550.6349941
CGCATTG251.9363166E-550.59128639
CTGTCGG150.00803006450.5912840
TCATTAT150.00803006450.5912840
GCTATGG150.00803006450.5912839
AGCTATG150.00803006450.5912838
GTCTAAT203.9333117E-450.59127839
ATATCAT150.00808503550.5040937
TAAGAGA150.00822368550.2874334
TAATCCG252.0374151E-550.15832542
CACGATT355.140646E-850.11543747
TACTATT507.2759576E-1250.0940246
TAGCCTG204.1386497E-450.0726243
CGAAGCG252.0685835E-550.0298812
TTCCGAA150.00839239350.02987348
GTAGCCG550.050.02987349
TCGCGCA355.2279574E-850.00853350
TAGCCGT650.050.0085350
GCCCGAA252.0790601E-549.987219
Produced by FastQC (version 0.11.5)