##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525826_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 117888 Sequences flagged as poor quality 0 Sequence length 56 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.47268593919652 38.0 36.0 39.0 33.0 40.0 2 35.31496844462541 38.0 35.0 39.0 27.0 40.0 3 35.088736767100976 38.0 35.0 39.0 26.0 40.0 4 35.12263334690554 38.0 35.0 39.0 26.0 40.0 5 33.758966137350704 37.0 33.0 39.0 21.0 40.0 6 35.50846566232356 38.0 36.0 40.0 28.0 40.0 7 35.684870385450594 38.0 36.0 39.0 30.0 40.0 8 35.65267881378936 38.0 36.0 39.0 29.0 40.0 9 35.70756141422367 38.0 36.0 39.0 30.0 40.0 10 35.13270222584148 38.0 35.0 39.0 27.0 40.0 11 34.92372421281216 38.0 35.0 39.0 27.0 40.0 12 33.73247489142237 38.0 33.0 39.0 21.0 40.0 13 33.21693471769815 38.0 32.0 39.0 18.0 40.0 14 33.844046891965256 38.0 33.0 39.0 22.0 40.0 15 32.731736902823016 38.0 31.0 39.0 15.0 40.0 16 32.306188925081436 37.0 30.0 39.0 17.0 40.0 17 31.31898072747014 36.0 28.0 39.0 13.0 40.0 18 30.648335708469055 35.0 26.0 39.0 12.0 40.0 19 31.922757193268186 36.0 29.0 39.0 16.0 40.0 20 32.45626357220413 36.0 30.0 39.0 19.0 40.0 21 32.53098703854506 38.0 30.0 39.0 17.0 40.0 22 31.773827700868623 36.0 29.0 39.0 14.0 40.0 23 32.447628257328994 37.0 30.0 39.0 17.0 40.0 24 31.85090085504886 36.0 29.0 39.0 15.0 40.0 25 32.2379207383279 37.0 30.0 39.0 17.0 40.0 26 32.72655401737242 38.0 31.0 39.0 17.0 40.0 27 32.86771342290988 38.0 32.0 39.0 18.0 40.0 28 32.8376509907709 38.0 32.0 39.0 18.0 40.0 29 32.42730388165038 37.0 31.0 39.0 16.0 40.0 30 32.201903501628664 37.0 31.0 39.0 15.0 40.0 31 32.92947543431053 38.0 33.0 39.0 17.0 40.0 32 32.74032132193268 37.0 32.0 39.0 16.0 40.0 33 32.52020561889251 37.0 32.0 39.0 14.0 40.0 34 32.33625135722041 37.0 31.0 39.0 12.0 40.0 35 32.21620521172638 37.0 31.0 39.0 10.0 40.0 36 31.59852571932682 36.0 30.0 38.0 4.0 40.0 37 31.3577802660152 36.0 30.0 38.0 4.0 40.0 38 30.720446525515744 35.0 29.0 38.0 4.0 40.0 39 29.83488565418024 35.0 27.0 38.0 4.0 40.0 40 27.675352877307276 33.0 18.0 38.0 4.0 39.0 41 25.863836862106407 31.0 14.0 37.0 4.0 39.0 42 26.732686957111834 32.0 19.0 37.0 4.0 39.0 43 26.657072814875136 32.0 18.0 37.0 4.0 39.0 44 25.74756548588491 31.0 14.0 37.0 4.0 39.0 45 26.680917480998914 32.0 19.0 37.0 4.0 39.0 46 24.625424131378935 29.0 12.0 36.0 4.0 39.0 47 23.601486156351793 28.0 8.0 35.0 4.0 39.0 48 24.277577022258416 29.0 10.0 36.0 4.0 39.0 49 23.24416395222584 28.0 4.0 35.0 4.0 39.0 50 23.865185599891422 29.0 4.0 35.0 4.0 39.0 51 24.095107220412594 30.0 4.0 36.0 4.0 39.0 52 23.903620385450598 30.0 4.0 36.0 4.0 39.0 53 23.701055238870794 30.0 4.0 36.0 4.0 39.0 54 23.35862852877307 29.0 4.0 35.0 4.0 38.0 55 23.00190859120521 29.0 4.0 35.0 4.0 38.0 56 22.60929017372421 28.0 4.0 36.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 3.4322417435549397 1 2 4.639692404262497 1 3 5.098013687828285 1 4 4.885519006718717 1 5 5.841558840034235 1 6 4.936779484067863 1 7 4.282999844348097 1 8 4.467058417881127 1 9 4.631602839811698 1 10 5.087562247745865 1 11 1.2988113590297772 1 12 -5.465323780649353 1 13 -17.982883264811825 1 14 -6.367691600094794 1 15 2.214066581994249 1 16 0.42485089199970005 1 17 -4.081624807460244 1 18 -2.812191371698649 1 19 1.1307877583767407 1 20 0.8639536514715971 1 21 6.950788973222114 1 22 7.969603751392565 1 23 7.146909907607842 1 24 7.575571070185781 1 25 6.999309665978043 1 26 6.706418960360722 1 27 6.493154402090649 1 28 6.772982438409702 1 29 7.089982303837164 1 30 6.888756341667346 1 31 5.765920972039421 1 32 5.457966350630688 1 33 5.618313849841147 1 34 5.490685466422569 1 35 5.9640564390427535 1 36 5.656323175576183 1 37 5.427850466365232 1 38 6.15639933072455 1 39 6.277549474908437 1 40 -7.405166448448849 1 41 2.032389864459031 1 42 0.8400543836912995 1 43 -6.409255644198211 1 44 -13.425608541051684 1 45 -0.09402556733773082 1 46 -2.42495391354311 1 47 -7.919814539428442 1 48 -2.3208416234463307 1 49 -12.271486920208016 1 50 -1.2151284541412828 1 51 5.706612110548843 1 52 5.291217136140887 1 53 5.245247770579322 1 54 5.343224208234819 1 55 5.148725235622827 1 56 8.183197282197327 2 1 3.774207769731497 2 2 5.057681822251915 2 3 5.284034294901524 2 4 5.2840152473202195 2 5 6.224182064762726 2 6 5.3369465684454696 2 7 4.677040501546649 2 8 4.7925944802592895 2 9 4.802307378937293 2 10 5.001235319313672 2 11 4.272634806537432 2 12 -0.22305143311384512 2 13 1.9580802550990626 2 14 5.239938586482765 2 15 5.554361764394695 2 16 5.655148859139775 2 17 7.711190564302495 2 18 5.083937840220038 2 19 6.46579193548618 2 20 6.168882640611113 2 21 7.6737073803510505 2 22 8.510121712963159 2 23 7.614467690955102 2 24 8.686403707334208 2 25 7.429551938325577 2 26 6.744291419285808 2 27 6.658567935925237 2 28 6.601999425321427 2 29 7.31805247927576 2 30 7.314821504574617 2 31 5.472687889332658 2 32 5.816362340605625 2 33 5.9098760887718065 2 34 6.2347678947155245 2 35 5.9192221310505495 2 36 6.603413122666133 2 37 6.3075497144855355 2 38 6.780737955063177 2 39 7.305953819102253 2 40 5.571998686611021 2 41 10.098109719652573 2 42 8.496974461664013 2 43 8.34930743015434 2 44 -3.3292565499628495 2 45 8.363785627315568 2 46 8.3127236136081 2 47 5.2723324948238535 2 48 8.304610896743032 2 49 1.6366166721061006 2 50 8.523384494897982 2 51 10.507224753266751 2 52 10.272837854603711 2 53 10.344662975257684 2 54 10.499448101300818 2 55 10.131459849936387 2 56 11.190994553152493 3 1 3.318221805973508 3 2 4.505553083594677 3 3 4.9222720885797315 3 4 4.8932758773062375 3 5 5.08132492190558 3 6 4.815275378380498 3 7 4.306149255007732 3 8 4.375400083790282 3 9 4.477828940469216 3 10 4.195522130180798 3 11 4.071165387451437 3 12 4.713538746999653 3 13 5.156013199145995 3 14 4.8805888592573865 3 15 0.5689737116491287 3 16 6.649830439313579 3 17 8.122326348495275 3 18 8.363429026446973 3 19 6.7332481407662215 3 20 6.539225596446297 3 21 6.965151303919324 3 22 7.77256105830406 3 23 6.928172217094861 3 24 7.418070236928195 3 25 7.18495945539112 3 26 6.535858708262424 3 27 5.976110496991115 3 28 5.9172117508964455 3 29 6.358351845999994 3 30 6.4620375585264185 3 31 5.143795710699546 3 32 5.494872086472885 3 33 5.456631578532477 3 34 4.9402809577415425 3 35 5.157372198216162 3 36 6.407618512363747 3 37 6.745579157696323 3 38 6.86278965887654 3 39 7.4669867987880885 3 40 9.328009323450676 3 41 12.07708277116448 3 42 10.484117044661513 3 43 10.691523072888117 3 44 11.461159328535931 3 45 10.761351216591006 3 46 12.519566281590667 3 47 13.269289481418149 3 48 12.113997633590913 3 49 13.171775813482864 3 50 11.885832064599438 3 51 11.9239256881024 3 52 11.021665883224482 3 53 11.687783297652622 3 54 11.569930871265452 3 55 10.890869889139175 3 56 10.913312726247867 4 1 3.5519855475092754 4 2 4.824877589447681 4 3 5.0406480515152765 4 4 5.199359162664138 4 5 5.187145027725688 4 6 4.924248155747058 4 7 4.518310342816491 4 8 4.422224109888923 4 9 4.574794151424065 4 10 4.773722091800444 4 11 5.129777663680294 4 12 5.903932693870281 4 13 6.159138456157265 4 14 5.996552343096518 4 15 6.358594568627499 4 16 7.284778488769401 4 17 8.891084744196679 4 18 9.782350538632734 4 19 7.296479766174009 4 20 7.687401159129632 4 21 7.996458703102373 4 22 8.28260848544993 4 23 7.095949172436583 4 24 8.157303178233676 4 25 7.4930440018176405 4 26 6.209899884894277 4 27 6.023647301004598 4 28 6.014697838019838 4 29 6.614348775572058 4 30 6.235456425209534 4 31 4.694910111554879 4 32 5.244933769177056 4 33 5.396648575544294 4 34 5.028418688366315 4 35 5.427158638987056 4 36 6.169550688803696 4 37 6.302258709194529 4 38 6.7119548862801075 4 39 6.829763342911779 4 40 1.757183871796208 4 41 10.65895628049913 4 42 10.084276048965595 4 43 10.30168838253529 4 44 11.083441862735558 4 45 9.919341182871126 4 46 12.783623084507571 4 47 13.09772932022068 4 48 12.84958444171658 4 49 12.731854767344199 4 50 13.211215182728669 4 51 12.46489671093871 4 52 11.801938383704236 4 53 12.302334932929647 4 54 12.89098249283521 4 55 12.380137098613634 4 56 11.545491907649847 5 1 3.4135100184087435 5 2 4.297678515373612 5 3 4.433174506541732 5 4 4.5558406441456185 5 5 5.50319992190558 5 6 4.9736033144772165 5 7 4.073204522710668 5 8 4.053589850629663 5 9 4.05768105618597 5 10 3.854740610318963 5 11 4.530902002304629 5 12 4.727841424029009 5 13 5.310428138696949 5 14 4.979439247858423 5 15 6.597929886087815 5 16 6.778743435859351 5 17 8.19432548493742 5 18 8.298554242336436 5 19 7.3393038402480855 5 20 6.584805259658733 5 21 7.168168359187025 5 22 7.23532012566157 5 23 6.776752743865153 5 24 7.447647093577594 5 25 6.9665063034049375 5 26 5.546788112407505 5 27 5.677913174020471 5 28 5.310994134316136 5 29 5.897913590386867 5 30 5.480496107749218 5 31 4.494265270285037 5 32 5.00237424536753 5 33 5.08249513639085 5 34 4.468481519054151 5 35 5.248504538457954 5 36 5.578198175576183 5 37 6.406012016072832 5 38 6.505357664057886 5 39 7.034128422276861 5 40 8.950553580790913 5 41 11.088767788435643 5 42 10.683895757960304 5 43 10.663295525392435 5 44 11.00622625426995 5 45 10.233825309855252 5 46 11.990799010433495 5 47 11.516132097998458 5 48 12.185645949653086 5 49 12.593461910201338 5 50 11.610438066326555 5 51 11.415128192420191 5 52 11.226706902222755 5 53 11.290843530813241 5 54 11.311700085427805 5 55 11.63236924147078 5 56 11.589721405004344 6 1 3.205680717333472 6 2 4.288437923975756 6 3 4.893523968907324 6 4 4.815249246296155 6 5 5.3985292229808515 6 6 4.706634228455712 6 7 4.343365813033252 6 8 4.378018614070527 6 9 4.264502292745107 6 10 4.523423406017891 6 11 4.973778346390652 6 12 5.558822606824712 6 13 6.648129751600173 6 14 5.70238951667563 6 15 6.283581767808247 6 16 7.323266285321711 6 17 8.755481398915908 6 18 8.679602629433212 6 19 8.047973195086797 6 20 6.621263592992069 6 21 7.76544658499348 6 22 7.680884641790602 6 23 6.9308186040802084 6 24 7.685550319384042 6 25 6.697857109856553 6 26 6.163219564020409 6 27 5.294512636386063 6 28 5.590564026789256 6 29 5.7194861710320275 6 30 6.272834817426634 6 31 4.83213489394095 6 32 4.807145750743874 6 33 5.081151050369346 6 34 5.009644153462752 6 35 5.423403732006346 6 36 6.462774788479408 6 37 5.98010475800832 6 38 6.269302556531006 6 39 6.763967131954278 6 40 3.079081806597369 6 41 10.683021820693707 6 42 9.304023446132351 6 43 10.304760579155875 6 44 10.211535394054902 6 45 9.164604879747724 6 46 11.40309739753027 6 47 12.300238280794154 6 48 11.556445680835878 6 49 11.955357071491665 6 50 10.632447474928703 6 51 9.98401260102234 6 52 10.255940773190499 6 53 9.93247659532937 6 54 9.607230999406298 6 55 9.920843703836372 6 56 9.59660984586456 7 1 3.547209342733069 7 2 4.725578290148384 7 3 5.1100889209561515 7 4 4.707867671172643 7 5 5.3461897867704415 7 6 4.989284620783522 7 7 4.366672991179136 7 8 4.4008026884675004 7 9 4.421898398528313 7 10 4.863411781490136 7 11 4.8611947950974255 7 12 5.762019351956937 7 13 6.3678042648230715 7 14 5.755368301912476 7 15 6.679658489691423 7 16 7.36339996739088 7 17 8.887681340793275 7 18 9.13830649458869 7 19 7.700283569977813 7 20 7.058200529929007 7 21 7.89398480062847 7 22 8.02552282836427 7 23 6.973684050171464 7 24 7.647421868352367 7 25 6.8630997718734115 7 26 6.015791490785887 7 27 6.116482993840297 7 28 5.689372512694515 7 29 5.89763205885534 7 30 5.550428540181649 7 31 3.940126756771523 7 32 4.546349470592755 7 33 4.912450091345807 7 34 4.736583996531628 7 35 5.027530439358859 7 36 5.764431283684289 7 37 6.585713592649409 7 38 7.003246177571402 7 39 7.235254548402988 7 40 7.485998400610736 7 41 10.51464053618339 7 42 10.775252739942285 7 43 10.797698678545586 7 44 11.15966093895464 7 45 10.32200098553093 7 46 12.610449911334396 7 47 13.00523682772819 7 48 11.784547976680116 7 49 12.840477675967104 7 50 12.204722976236464 7 51 11.029261075303069 7 52 11.534871316637169 7 53 10.781299611894323 7 54 11.65834986020258 7 55 11.620291538768075 7 56 10.686089648247588 8 1 3.5170026654675652 8 2 4.488058417334393 8 3 4.911360781051542 8 4 4.904370055910327 8 5 5.457795510140873 8 6 5.022194000751732 8 7 4.143302561926355 8 8 4.3159060271002545 8 9 4.4991638993232215 8 10 4.572632767181709 8 11 4.954676512108556 8 12 5.908601227950577 8 13 6.443638923010674 8 14 5.895431894917245 8 15 6.421152925303506 8 16 7.216182161349547 8 17 8.733786269251144 8 18 9.019387575669775 8 19 7.441142075542203 8 20 6.5408714361293185 8 21 7.191759045461534 8 22 7.893408360955686 8 23 7.118358136022017 8 24 8.05696081906779 8 25 7.245611695561802 8 26 6.284471935936914 8 27 5.912349448530275 8 28 6.646288251963195 8 29 6.038967511955494 8 30 6.270937284219805 8 31 4.8889343879321 8 32 5.560339931642044 8 33 4.755534352077124 8 34 4.778714362191401 8 35 5.124668754144228 8 36 5.691617293223246 8 37 5.789529172935577 8 38 6.1191444287637715 8 39 6.434618618355291 8 40 2.0112766200066012 8 41 10.586500631572893 8 42 9.756015855999525 8 43 9.681800427353217 8 44 10.06713311701505 8 45 9.587501780443485 8 46 11.960529402590357 8 47 12.634330627410222 8 48 12.075168008476616 8 49 12.5021628905935 8 50 11.808538556522631 8 51 11.018927212028032 8 52 11.442025529673739 8 53 12.076689119048538 8 54 11.914947634447412 8 55 11.9171118885296 8 56 11.372258169710225 9 1 3.7743643083495684 9 2 4.908594442395305 9 3 5.3497178990466665 9 4 4.929361354204794 9 5 5.880141489952916 9 6 5.185542417041319 9 7 4.558165074978916 9 8 4.399651241162211 9 9 4.666932782024233 9 10 4.862260334184846 9 11 5.2159365190699205 9 12 6.428963218900805 9 13 6.679942438499708 9 14 6.102497679811087 9 15 7.15731721350399 9 16 7.655931552230154 9 17 9.032219962254974 9 18 9.298677516498174 9 19 8.104281650898976 9 20 7.361894756700156 9 21 7.974042372993733 9 22 8.502701782466307 9 23 7.817279124535766 9 24 8.129846304622959 9 25 7.7019291337796965 9 26 6.72735394080987 9 27 6.407511300253216 9 28 6.328745613606078 9 29 6.389715858631444 9 30 7.379534569287678 9 31 5.563699441882672 9 32 5.764516750298501 9 33 6.444674623570339 9 34 5.786128215306611 9 35 6.4291936410220565 9 36 7.330879388593935 9 37 7.983890467749365 9 38 7.460146865241317 9 39 8.374611656990862 9 40 4.888269310573953 9 41 10.502198508356745 9 42 10.878083381234465 9 43 10.525413375491055 9 44 11.400025563934644 9 45 10.632124126423296 9 46 12.409746249092276 9 47 12.828169819891947 9 48 12.813142251428232 9 49 13.421062995013965 9 50 13.10467499926541 9 51 11.583459060270286 9 52 11.482192602419996 9 53 11.699035098860577 9 54 12.386928142627017 9 55 12.20938477401516 9 56 12.35053871269665 10 1 3.192475661683595 10 2 4.394078369174778 10 3 4.8757914656060635 10 4 4.493706141221644 10 5 5.069629015472831 10 6 4.56835843143628 10 7 3.8093156338217824 10 8 4.057701692734923 10 9 4.380140851507605 10 10 4.2396090313716 10 11 4.427466329790008 10 12 5.67502709654363 10 13 5.537584571445485 10 14 4.8428347156946785 10 15 6.886581201877288 10 16 7.08566960544999 10 17 7.848199753943266 10 18 8.860323248184393 10 19 6.645407641417677 10 20 6.688515054980368 10 21 6.430321136964803 10 22 7.677206090573851 10 23 7.047494702929484 10 24 8.031711421062973 10 25 6.116268730305524 10 26 6.326302001296391 10 27 5.615961419634505 10 28 5.539064309754732 10 29 5.873608034831317 10 30 5.502791429386647 10 31 5.128494071454629 10 32 4.820539450045896 10 33 5.466267066215412 10 34 5.028697162329003 10 35 5.133647082317605 10 36 5.97796060247677 10 37 6.486329998528976 10 38 7.255814535402916 10 39 7.792169357949373 10 40 9.565593785469279 10 41 10.418354776739736 10 42 10.623680114685452 10 43 10.520568917205296 10 44 11.270297891696853 10 45 9.736383789387414 10 46 10.978554858386712 10 47 11.898898910863956 10 48 11.141146680647239 10 49 12.2021972903183 10 50 11.194228271004913 10 51 11.314799245051766 10 52 11.03975514783679 10 53 11.274487536661198 10 54 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2.717500619767396 115 17 3.936423186086845 115 18 4.629732403255979 115 19 3.6722779781791175 115 20 3.2706888803483913 115 21 5.0893249683824315 115 22 6.991785642902951 115 23 6.250711077198488 115 24 6.32552065679598 115 25 5.783137050531376 115 26 4.583964836545434 115 27 4.893789610802084 115 28 4.673063099833374 115 29 4.719393475444338 115 30 4.478340935335424 115 31 3.870881649595379 115 32 4.451009302838795 115 33 3.5753112283448765 115 34 3.603359392617371 115 35 4.177204251101632 115 36 5.242530072127906 115 37 5.537297935613065 115 38 5.869043008885473 115 39 6.153381295840081 115 40 9.127098121020804 115 41 8.540456006826446 115 42 7.566259263707433 115 43 5.490522536886687 115 44 -0.24341455032774917 115 45 6.158394275372494 115 46 2.689434067904763 115 47 -7.193458419242921 115 48 1.0730381910323956 115 49 -3.9298857909480844 115 50 2.2873418019587355 115 51 3.621665548742026 115 52 3.0675833390043685 115 53 3.5036668066753087 115 54 3.6243796831289536 115 55 3.031614931125951 115 56 6.258363646383653 116 1 3.051253812734984 116 2 4.233073018919711 116 3 4.559504293775774 116 4 4.304954119322922 116 5 4.655793007011962 116 6 4.423403846392112 116 7 4.137920834767407 116 8 3.9501988931828578 116 9 4.288066694483838 116 10 3.8950774897515927 116 11 3.794643137056404 116 12 3.16600631764603 116 13 4.728202961391979 116 14 4.454505737220124 116 15 -3.160824546536297 116 16 4.678676946497649 116 17 6.213385768625361 116 18 5.684635802620129 116 19 5.66133398209206 116 20 4.773745862495613 116 21 6.2979334301090155 116 22 7.078848494455894 116 23 6.326952211950264 116 24 7.179030072300996 116 25 6.418744778582248 116 26 5.309753537939422 116 27 5.045599344233242 116 28 4.821632432188476 116 29 5.712851533649282 116 30 5.222960646756313 116 31 3.9032856603559587 116 32 4.472897295012924 116 33 4.638346200220642 116 34 4.6888937530967 116 35 4.085494786684908 116 36 5.571216792597461 116 37 6.474385243023189 116 38 6.12803053639831 116 39 6.7601922520640905 116 40 9.125753048876025 116 41 8.266183568577485 116 42 6.3322778501588886 116 43 2.3736235395768297 116 44 1.8792315734188882 116 45 7.2165380758127 116 46 1.8726695778093827 116 47 -5.918819143136293 116 48 0.9178046375963476 116 49 -2.316777451500787 116 50 1.704742144340738 116 51 4.074037766888274 116 52 3.6679746327192113 116 53 4.063339098189129 116 54 4.0296743762079466 116 55 4.154199915229647 116 56 6.411640731245477 117 1 2.99403886456259 117 2 3.879008643578736 117 3 4.329007839875068 117 4 4.065456028761005 117 5 4.28324799882866 117 6 4.118234724733632 117 7 3.9161532406593906 117 8 3.9514263890912034 117 9 3.838530415160328 117 10 4.067881635959992 117 11 0.5132737971764243 117 12 -7.138344473406885 117 13 -12.18396288694408 117 14 -2.223685752141577 117 15 -8.750227165194232 117 16 -7.188003359012445 117 17 0.5677229208348535 117 18 -3.190227808945611 117 19 0.28401537870962557 117 20 -0.6063005095720371 117 21 5.5243382309819005 117 22 6.24974320258465 117 23 5.731480307967722 117 24 6.248929144859645 117 25 5.541426175199813 117 26 5.764336189330585 117 27 5.5761503535076535 117 28 5.387917211239216 117 29 5.9067276929509696 117 30 5.706457646210758 117 31 4.584409501054271 117 32 4.448688347931633 117 33 4.944674623570339 117 34 4.689234724182356 117 35 4.8041936410220565 117 36 5.617861637114643 117 37 6.086701118636935 117 38 6.018578817904039 117 39 6.893102781251219 117 40 9.25143499104733 117 41 7.477790224333077 117 42 6.1169887066782564 117 43 -4.629172423325517 117 44 -0.30707502778133033 117 45 5.257863771393712 117 46 -0.784040733156246 117 47 -1.0956467481553887 117 48 1.020982488114626 117 49 -3.3304162949268665 117 50 -0.05582795931447393 117 51 5.314967936009932 117 52 5.009559466325321 117 53 4.525218530813241 117 54 5.746395598248316 117 55 5.14799424147078 117 56 7.706307943465884 118 1 2.6581791360558 118 2 3.9115878290990977 118 3 3.9819490163456592 118 4 3.986722997086794 118 5 4.504854333670284 118 6 3.9339587066340798 118 7 3.690361385455766 118 8 3.504141321217901 118 9 3.7344580169702795 118 10 3.9785151201228857 118 11 0.21350004152031943 118 12 -9.020810536755302 118 13 -8.715184606401092 118 14 -8.816332810965108 118 15 -8.837670604108261 118 16 -11.607347250415163 118 17 0.239668622192319 118 18 -5.415906541977286 118 19 -1.0647402773989718 118 20 -1.2473638579883257 118 21 4.927053163108592 118 22 6.287526008014513 118 23 4.706593430139662 118 24 5.468725524950138 118 25 4.716199930855918 118 26 5.29623664181927 118 27 5.288820036765575 118 28 4.710994134316135 118 29 5.233085159014319 118 30 5.353290225396279 118 31 4.394816740873271 118 32 4.4191634610538095 118 33 4.137887293253595 118 34 4.419890832779636 118 35 4.095256989438351 118 36 5.268087881458534 118 37 5.3659997611708725 118 38 6.360320899352001 118 39 7.164030383061171 118 40 8.458335443536011 118 41 7.633559455102308 118 42 7.773662914823053 118 43 0.9700357214708646 118 44 0.9024272346621096 118 45 6.9992664863258405 118 46 0.87817646141389 118 47 -1.9244929020015427 118 48 0.3810503614177918 118 49 -1.074307697641796 118 50 2.337950321228515 118 51 6.518927212028032 118 52 6.124378470850207 118 53 4.57080676610736 118 54 6.2796535168003516 118 55 6.44064130029431 118 56 8.336964052063166 119 1 2.4871707326944588 119 2 3.8013987534688454 119 3 4.114722125589353 119 4 3.9754835012884726 119 5 3.9116820647627257 119 6 3.469883076381983 119 7 2.9422521417582885 119 8 3.1451077077725174 119 9 3.030151294281204 119 10 3.339859657938014 119 11 1.6945924784951103 119 12 -5.095075242637655 119 13 -2.2148694803506714 119 14 -5.1790428949987195 119 15 -7.222754637721707 119 16 -7.735393468902558 119 17 1.1824207230326564 119 18 -5.769898138615943 119 19 0.15626812596237016 119 20 -0.34599831176984 119 21 4.74480526394893 119 22 5.681748697090139 119 23 4.750711077198488 119 24 4.185742331672827 119 25 5.065464636738273 119 26 5.196341683836074 119 27 4.569282221639522 119 28 4.810994134316136 119 29 5.610711209434488 119 30 5.766210393463503 119 31 4.326854555999322 119 32 4.78660043584372 119 33 4.040828469724186 119 34 4.141844614292246 119 35 4.517105728934144 119 36 5.781323175576183 119 37 6.424613206549022 119 38 6.025446949772171 119 39 6.531152231800672 119 40 8.47808334269568 119 41 6.59248802653088 119 42 5.465889805579355 119 43 -3.0762878079409006 119 44 -2.2452618409681406 119 45 4.832039595569537 119 46 -0.7160462276617423 119 47 -1.2673500448586843 119 48 -3.9103361932040563 119 49 -1.2473118993224688 119 50 1.6543368758503618 119 51 6.880902001943998 119 52 6.653790235556091 119 53 6.650218530813241 119 54 6.194884609237327 119 55 6.906922812899353 119 56 8.837489262147201 120 1 2.365742161265885 120 2 3.560327324897422 120 3 3.676626887494116 120 4 3.7284596917646695 120 5 4.3956106361912965 120 6 3.444883076381984 120 7 3.1648711893773367 120 8 3.467726755391567 120 9 3.517651294281208 120 10 3.34938346746182 120 11 0.230306764209395 120 12 -7.527813337875749 120 13 -6.605940908922101 120 14 -7.146304799760625 120 15 0.7236739337068663 120 16 -8.55860775461684 120 17 0.6603969135088441 120 18 -5.5044219481397505 120 19 -0.3913509216566773 120 20 -0.29778402605555243 120 21 3.9858766925203604 120 22 5.370439173280619 120 23 4.829282505769918 120 24 5.1678851888156885 120 25 4.480345589119228 120 26 4.161222636217026 120 27 3.4966631740204726 120 28 5.120517943839946 120 29 4.667258828482108 120 30 4.4227580125111245 120 31 3.7012593179040856 120 32 3.292552816796107 120 33 4.109876088771802 120 34 3.278154138101769 120 35 3.821867633696055 120 36 5.046799366052376 120 37 4.913898920834736 120 38 5.489732664057886 120 39 5.947223660372099 120 40 7.751892866505198 120 41 -2.128345306802455 120 42 -0.6162530515635041 120 43 -3.2506925698456612 120 44 -13.039309460015762 120 45 -1.3155794520495085 120 46 -7.014260513376026 120 47 -6.0387786162872565 120 48 -3.9281933360611987 120 49 -13.763383327893898 120 50 -3.1242345527210666 120 51 1.3844734305154276 120 52 1.025218806984661 120 53 1.8906947212894316 120 54 1.312146513999231 120 55 2.0658513843279245 120 56 4.661894024051964 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 4982.0 5 74.0 6 120.0 7 460.0 8 656.0 9 581.0 10 424.0 11 364.0 12 303.0 13 291.0 14 379.0 15 455.0 16 551.0 17 703.0 18 858.0 19 1058.0 20 1352.0 21 1729.0 22 2107.0 23 2375.0 24 2852.0 25 3167.0 26 3397.0 27 3685.0 28 3992.0 29 4294.0 30 4827.0 31 5389.0 32 6349.0 33 6965.0 34 7815.0 35 8306.0 36 8857.0 37 9879.0 38 9888.0 39 8401.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 99.08981406080348 0.7286577090119435 0.12639115092290987 0.0551370792616721 8 99.35107899022802 0.35796688382193265 0.11621199782844735 0.17474212812160694 9 97.88185195867194 0.8694840778379112 0.5115111209134249 0.7371528425767266 10 52.256378935939196 27.318302117263844 11.134296959826276 9.291021986970684 11 37.14457790445168 23.84805917480999 19.135111292073834 19.872251628664493 12 30.048011672095548 17.408896579804562 22.446729098805644 30.096362649294246 13 25.135722041259502 15.06938789359392 24.371437296416936 35.42345276872964 14 24.243349619978286 23.169448968512487 27.802660152008684 24.784541259500543 15 31.739738408280893 18.551866441568183 25.0345321532716 24.67386299687932 16 32.638735634135166 22.167144821498294 22.7935559441153 22.400563600251246 17 29.245114925461777 19.96792777810774 29.337598316632587 21.449358979797893 18 26.614244028230182 20.464339033659066 25.698968512486424 27.222448425624325 19 28.93964083239977 21.514410836134026 22.14414230912856 27.401806022337645 20 26.339031701952393 24.889387106910227 27.297818314607696 21.473762876529683 21 26.08407980456026 20.283659066232357 27.886638165038 25.745622964169378 22 24.329024158523342 20.214101520086864 28.443946796959825 27.01292752442997 23 24.580109934853418 21.227775515743758 26.447136264929426 27.744978284473397 24 25.66575891001332 20.848710052344472 26.516674726188356 26.968856311453855 25 27.31668830011962 22.633682013692702 25.65133660804425 24.398293078143425 26 30.415704780010866 21.182441607821836 21.439604834329167 26.962248777838134 27 23.03107488537952 24.13143148242486 22.244014263881816 30.5934793683138 28 24.743396162394177 25.867363384965138 21.916936701559134 27.472303751081554 29 31.008608309185316 23.430237004682308 21.83689251085599 23.724262175276394 30 28.69583669312057 20.937062343504646 27.20281797733735 23.16428298603743 31 28.889209389926272 26.117535271610485 22.568740402643567 22.424514935819666 32 32.288829493869585 23.522973144966272 22.430104789784057 21.758092571380086 33 31.216342478552622 23.395905886350125 21.85084515416631 23.536906480930945 34 25.07628364767579 25.250524848495147 27.77064758229709 21.902543921531965 35 23.308775418291823 24.708321649204205 31.33810895556632 20.644793976937653 36 26.08016702170094 28.621137410993896 24.247850698894162 21.050844868411005 37 27.43107183822592 24.903351884107227 26.603509070053953 21.0620672076129 38 30.215827338129497 25.018283245743834 22.56067486436383 22.205214551762843 39 27.484391590571246 23.75196432363559 20.961562964535997 27.802081121257167 40 23.09824216243959 18.605705487361533 25.48222916042941 32.81382318976947 41 20.053148171960054 16.315631563156316 22.095066649522092 41.536153615361535 42 26.398815601388602 24.960009529644 23.33061057790484 25.310564291062555 43 22.06625491297024 20.028754700287546 28.017116703248092 29.88787368349412 44 22.949031504853128 22.531466505321823 24.715587104911076 29.803914884913972 45 25.615344720576104 22.194920802264978 27.097273353341777 25.092461123817134 46 22.63307944342794 25.58784732564572 23.76239308965576 28.016680141270584 47 19.77475787750648 23.924942027008594 24.51660755694994 31.783692538534993 48 25.100589104120335 24.35359828871195 26.120910649712236 24.42490195745548 49 23.37294464906983 23.982851164096324 24.59190704242125 28.05229714441259 50 25.010625456894648 23.470359225446693 25.8385610581255 25.680454259533157 51 23.232357622545475 25.630920867840697 27.23450254070663 23.9022189689072 52 23.15891472868217 24.527403780769756 28.153474772201818 24.160206718346252 53 22.25209283332199 24.190090519294902 29.195024841761384 24.362791805621725 54 22.37814575732061 23.597751299126546 28.140229122547396 25.883873821005455 55 23.009775645116008 23.82228574831755 26.8962114057701 26.271727200796345 56 24.475179476710558 21.71157837433228 28.1701881528359 25.643053996121264 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.5 9 2.0 10 4.0 11 6.0 12 15.0 13 62.5 14 101.0 15 110.0 16 119.0 17 152.5 18 186.0 19 189.0 20 192.0 21 194.5 22 194.0 23 191.0 24 209.5 25 228.0 26 239.5 27 251.0 28 317.5 29 452.5 30 521.0 31 572.0 32 623.0 33 703.0 34 783.0 35 889.5 36 996.0 37 1237.0 38 1662.0 39 1846.0 40 1896.0 41 1946.0 42 2135.5 43 2325.0 44 2675.5 45 3026.0 46 3732.0 47 6200.0 48 7962.0 49 7715.5 50 7469.0 51 6782.5 52 6096.0 53 6226.0 54 6561.0 55 6766.0 56 7166.0 57 7566.0 58 7599.5 59 7633.0 60 8069.0 61 8505.0 62 8630.0 63 8421.5 64 8088.0 65 7170.5 66 6253.0 67 5851.5 68 5450.0 69 4497.0 70 3544.0 71 3050.5 72 2198.5 73 1840.0 74 1617.0 75 1394.0 76 1037.0 77 680.0 78 568.0 79 374.0 80 292.0 81 284.0 82 276.0 83 172.0 84 68.0 85 42.0 86 16.0 87 8.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0016965255157437569 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5140472312703582 16 0.062771444082519 17 0.024599619978284474 18 0.0 19 0.050895765472312705 20 0.11451547231270359 21 0.0 22 0.0 23 0.0 24 0.012723941368078176 25 0.012723941368078176 26 0.05428881650380022 27 0.09161237785016287 28 0.0016965255157437569 29 0.17898344191096632 30 0.061923181324647115 31 0.014420466883821934 32 0.02799267100977199 33 0.1340255157437568 34 0.1993417480998914 35 0.1755903908794788 36 0.05004750271444083 37 0.16371471226927253 38 0.24938925081433222 39 0.13826682953311617 40 0.8338422909880565 41 1.045907980456026 42 0.30537459283387625 43 0.2901058631921824 44 0.45891015200868623 45 0.22987920738327905 46 0.3248846362649294 47 0.502171552660152 48 0.06955754614549403 49 0.279078447339848 50 0.2086726384364821 51 0.34015336590662326 52 0.20358306188925082 53 0.2918023887079262 54 0.2621131921824104 55 0.2977402280130293 56 0.2748371335504886 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 117888.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.80629071661238 #Duplication Level Percentage of deduplicated Percentage of total 1 88.55299996456037 63.58662459283387 2 6.872925305076135 9.870385450597178 3 1.9468169306918996 4.193811074918567 4 0.8505510862246164 2.44299674267101 5 0.46071517170500054 1.654112377850163 6 0.2858796706477183 1.2316775244299674 7 0.18074210582273098 0.9084894136807818 8 0.1393958724645899 0.8007600434310532 9 0.09450567624717959 0.6107491856677525 >10 0.5493142431867314 7.27979098805646 >50 0.03662094954578209 1.7414834419109664 >100 0.02598906096797439 3.48635993485342 >500 0.00236264190617949 0.9042480998914224 >1k 0.001181320953089745 1.2885111292073834 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGGGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1519 1.2885111292073834 No Hit AGGGTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 561 0.4758754071661238 No Hit AGGGTCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGC 505 0.4283726927252986 No Hit AGGGTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 437 0.37069082519001084 No Hit AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 367 0.31131243213897936 No Hit AGGGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 341 0.28925760043431054 No Hit AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 274 0.2324239956568947 No Hit AGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGT 246 0.2086726384364821 No Hit AGGGTCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 212 0.17983170466883822 No Hit AGGGTCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 189 0.16032166123778502 No Hit AGGGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 184 0.15608034744842564 No Hit AGGGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 179 0.15183903365906623 No Hit AGGGTCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 171 0.1450529315960912 No Hit AGGGTCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 161 0.1365703040173724 No Hit AGGGTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 149 0.12639115092290987 No Hit AGGGTCGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCG 146 0.12384636264929423 No Hit AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 141 0.11960504885993486 No Hit AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 120 0.10179153094462541 No Hit AGGGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 119 0.10094326818675353 No Hit AGGGTCGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 118 0.10009500542888165 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 8.482627578718784E-4 0.0 0.0 0.0 0.0 9 8.482627578718784E-4 0.0 0.0 0.0 0.0 10 0.00508957654723127 0.0 0.0 0.0 0.0 11 0.01017915309446254 0.0 0.0 0.0 0.0 12 0.012723941368078176 0.0 0.0 0.0 0.0 13 0.01696525515743757 0.0 0.0 0.0 0.0 14 0.028840933767643864 0.0 0.0 0.0 0.0 15 0.0313857220412595 0.0 0.0 0.0 0.0 16 0.034778773072747016 0.0 0.0 0.0 0.0 17 0.03732356134636265 0.0 0.0 0.0 0.0 18 0.04071661237785016 0.0 0.0 0.0 0.0 19 0.044109663409337675 0.0 0.0 0.0 0.0 20 0.04665445168295331 0.0 0.0 0.0 0.0 21 0.04835097719869707 0.0 0.0 0.0 0.0 22 0.05004750271444083 0.0 0.0 0.0 0.0 23 0.05259229098805646 0.0 0.0 0.0 0.0 24 0.05513707926167209 0.0 0.0 0.0 0.0 25 0.059378393051031486 0.0 0.0 0.0 0.0 26 0.062771444082519 0.0 0.0 0.0 0.0 27 0.07040580890336591 0.0 0.0 0.0 0.0 28 0.0746471226927253 0.0 0.0 0.0 0.0 29 0.07549538545059718 0.0 0.0 0.0 0.0 30 0.07549538545059718 0.0 0.0 0.0 0.0 31 0.07804017372421282 0.0 0.0 0.0 0.0 32 0.08143322475570032 0.0 0.0 0.0 0.0 33 0.0822814875135722 0.0 0.0 0.0 0.0 34 0.0822814875135722 0.0 0.0 0.0 0.0 35 0.08397801302931596 0.0 0.0 0.0 0.0 36 0.08482627578718784 0.0 0.0 0.0 0.0 37 0.08821932681867535 0.0 0.0 0.0 0.0 38 0.08906758957654723 0.0 0.0 0.0 0.0 39 0.08991585233441911 0.0 0.0 0.0 0.0 40 0.09076411509229099 0.0 0.0 0.0 0.0 41 0.09246064060803474 0.0 0.0 0.0 0.0 42 0.09670195439739414 0.0 0.0 0.0 0.0 43 0.10179153094462541 0.0 0.0 0.0 0.0 44 0.11027415852334418 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTATA 15 0.0080026835 50.63499 41 ACCCAAC 15 0.0080026835 50.63499 41 CGCATTG 25 1.9363166E-5 50.591286 39 CTGTCGG 15 0.008030064 50.59128 40 TCATTAT 15 0.008030064 50.59128 40 GCTATGG 15 0.008030064 50.59128 39 AGCTATG 15 0.008030064 50.59128 38 GTCTAAT 20 3.9333117E-4 50.591278 39 ATATCAT 15 0.008085035 50.50409 37 TAAGAGA 15 0.008223685 50.28743 34 TAATCCG 25 2.0374151E-5 50.158325 42 CACGATT 35 5.140646E-8 50.115437 47 TACTATT 50 7.2759576E-12 50.09402 46 TAGCCTG 20 4.1386497E-4 50.07262 43 CGAAGCG 25 2.0685835E-5 50.02988 12 TTCCGAA 15 0.008392393 50.029873 48 GTAGCCG 55 0.0 50.029873 49 TCGCGCA 35 5.2279574E-8 50.008533 50 TAGCCGT 65 0.0 50.00853 50 GCCCGAA 25 2.0790601E-5 49.98721 9 >>END_MODULE