FastQCFastQC Report
Fri 17 Jun 2016
SRR1525825_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525825_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6026
Sequences flagged as poor quality0
Sequence length40
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC270.4480584135413209No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG220.36508463325589113No Hit
AGGGTCGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC180.2987056090275473No Hit
AGGGTCGGGGACACGCAGGGATGAGATGGGGAAAAAAAAA150.24892134085628942No Hit
AGGGTCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAA140.23232658479920343No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC120.19913707268503153No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG120.19913707268503153No Hit
AGGGTCGGGGACACGCAGGGATGAGATGGCCCAAAAAAAA90.14935280451377364No Hit
AGGGTCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAA80.1327580484566877No Hit
AGGGTCGGGGAGTGCAGTGCTCGGGTCGGGGACACGCAGG80.1327580484566877No Hit
AGGGTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT80.1327580484566877No Hit
AGGGTCGGGAACCAGTCTCTTGTCTCAGGCTCTCTGCCTT80.1327580484566877No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG80.1327580484566877No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC80.1327580484566877No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC80.1327580484566877No Hit
AGGGTCGGGAGTGCAGTGCTAGGGTCGGGGACACGCAGGG70.11616329239960171No Hit
AGGGTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT70.11616329239960171No Hit
AGGGTCGGGGGGGCTGGTGAGCTGGCTCAGTGGGTAAGAG70.11616329239960171No Hit
AGGGTCGGGCTCGTCTTAATCAGCAACATCTTTTGATACA70.11616329239960171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGAG454.2000465E-933.96666719
ACACGCA358.7804256E-733.96666711
CGGGGGT251.8089853E-433.9666676
CGGGGGC200.002587117333.9666676
CGGGGCT200.002587117333.9666676
GCAGGGA358.7804256E-733.96666715
GGACACG406.0865204E-833.9666679
AGGGATG358.7804256E-733.96666717
GATGAGA454.2000465E-933.96666720
GACACGC454.2000465E-933.96666710
CAGGGAT358.7804256E-733.96666716
CGGGAGT251.8089853E-433.9666676
CGGGAGC200.002587117333.9666676
AGATGGG358.7804256E-733.96666724
GGAAGCA200.002587117333.9666678
GGGATGA454.2000465E-933.96666718
GGGAAGT251.8089853E-433.9666677
GGGAAGC251.8089853E-433.9666677
GTCGGGT502.8921932E-1033.9666674
ATGAGAT454.2000465E-933.96666721
Produced by FastQC (version 0.11.5)