##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525825_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6026
Sequences flagged as poor quality	0
Sequence length	40
%GC	54
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.696647859276467	28.0	19.0	33.0	4.0	35.0
2	21.477928974444076	25.0	11.0	30.0	4.0	34.0
3	20.652837703285762	23.0	10.0	30.0	4.0	33.0
4	19.701460338533025	22.0	9.0	29.0	4.0	34.0
5	20.576169930302026	23.0	10.0	30.0	4.0	34.0
6	15.130766677729838	15.0	8.0	18.0	4.0	30.0
7	19.286757384666444	21.0	4.0	30.0	4.0	33.0
8	20.787089279787587	24.0	4.0	31.0	4.0	35.0
9	18.70278791901759	20.0	4.0	29.0	4.0	33.0
10	19.158479920345172	20.0	4.0	30.0	4.0	34.0
11	19.618818453368736	20.0	4.0	31.0	4.0	35.0
12	18.75307002987056	19.0	4.0	30.0	4.0	35.0
13	19.518254231662794	22.0	4.0	31.0	4.0	35.0
14	19.45967474278128	22.0	4.0	31.0	4.0	35.0
15	18.963989379356125	20.0	4.0	31.0	4.0	35.0
16	18.769166943245935	19.0	4.0	31.0	4.0	35.0
17	19.379688018586126	22.0	4.0	32.0	4.0	35.0
18	19.530202456023897	22.0	4.0	32.0	4.0	36.0
19	17.758878194490542	17.0	4.0	30.0	4.0	35.0
20	18.464653169598407	20.0	4.0	31.0	4.0	35.0
21	16.699468967806173	14.0	4.0	30.0	4.0	35.0
22	16.47676734152008	14.0	4.0	30.0	4.0	34.0
23	16.727016262860936	15.0	4.0	30.0	4.0	34.0
24	15.896614669764354	11.0	4.0	29.0	4.0	34.0
25	15.232326584799203	4.0	4.0	29.0	4.0	34.0
26	13.085794888815135	4.0	4.0	25.0	4.0	33.0
27	13.196315964155326	4.0	4.0	25.0	4.0	33.0
28	12.842515765018254	4.0	4.0	25.0	4.0	33.0
29	12.624294722867575	4.0	4.0	25.0	4.0	33.0
30	11.96150016594756	4.0	4.0	23.0	4.0	32.0
31	11.252240292067707	4.0	4.0	20.0	4.0	32.0
32	10.745602389644873	4.0	4.0	17.0	4.0	31.0
33	10.114171921672751	4.0	4.0	15.0	4.0	31.0
34	9.61566544971789	4.0	4.0	11.0	4.0	30.0
35	9.195486226352473	4.0	4.0	4.0	4.0	29.0
36	8.927978758712246	4.0	4.0	4.0	4.0	30.0
37	8.816296050448058	4.0	4.0	4.0	4.0	30.0
38	8.29920345170926	4.0	4.0	4.0	4.0	27.0
39	8.21423830069698	4.0	4.0	4.0	4.0	26.0
40	8.118984400929307	4.0	4.0	4.0	4.0	26.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	5.258352003746744
1	2	5.737443988759779
1	3	-0.1750958836485097
1	4	9.010005442900187
1	5	5.298383268777997
1	6	4.690170502012608
1	7	9.0542685628212
1	8	6.82145673527253
1	9	0.010337712969295865
1	10	4.912856320093159
1	11	5.1820773524720884
1	12	4.541389774942406
1	13	1.5530043543201444
1	14	4.089625895546952
1	15	7.30151894888737
1	16	0.1897182982709289
1	17	-0.39120022277917244
1	18	-0.6971060859218738
1	19	-9.241401167059063
1	20	0.9766604010025048
1	21	5.699922786764894
1	22	3.8924929115718605
1	23	0.2057001721475462
1	24	0.8424391154654352
1	25	1.47917774233564
1	26	7.580660299739243
1	27	1.8264123060175699
1	28	6.672105136578814
1	29	2.388042074884181
1	30	0.7030109997215241
1	31	2.3278398015240143
1	32	1.3254908103592289
1	33	0.998658261816157
1	34	-2.893443836864889
1	35	-1.0796590592643218
1	36	-0.00720772891825483
1	37	-0.37858820029872753
1	38	0.3454263816105909
1	39	-0.551428761297184
1	40	-2.0625028480291654
3	1	-0.7416479962532563
3	2	-6.16255601124022
3	3	-11.275095883648511
3	4	-10.089994557099814
3	5	-13.101616731222002
3	6	14.990170502012608
3	7	3.254268562821199
3	8	4.82145673527253
3	9	0.5103377129692959
3	10	-5.48714367990684
3	11	5.982077352472089
3	12	14.641389774942407
3	13	15.653004354320146
3	14	11.48962589554695
3	15	14.701518948887369
3	16	-7.310281701729071
3	17	7.508799777220826
3	18	6.202893914078125
3	19	7.5585988329409375
3	20	-4.423339598997494
3	21	5.599922786764893
3	22	16.39249291157186
3	23	0.9057001721475455
3	24	5.042439115465434
3	25	17.27917774233564
3	26	-8.219339700260758
3	27	-8.67358769398243
3	28	-8.227894863421184
3	29	-7.91195792511582
3	30	-7.196989000278476
3	31	-5.9721601984759864
3	32	-5.5745091896407715
3	33	-4.801341738183844
3	34	-4.693443836864889
3	35	-4.279659059264322
3	36	-3.8072077289182547
3	37	-3.778588200298728
3	38	-3.454573618389409
3	39	-3.351428761297184
3	40	-3.162502848029165
5	1	-8.741647996253256
5	2	-9.051444900129109
5	3	-5.8306514392040665
5	4	-6.75666122376648
5	5	-5.212727842333113
5	6	-6.787607275765169
5	7	-10.7457314371788
5	8	-13.400765486949691
5	9	-11.378551175919593
5	10	-10.264921457684618
5	11	-7.4623670919723555
5	12	-8.469721336168703
5	13	-11.90255120123541
5	14	-8.73259632667527
5	15	-7.1873699400015205
5	16	-7.08805947950685
5	17	-8.268978000556952
5	18	-6.130439419255209
5	19	-4.996956722614618
5	20	-6.645561821219715
5	21	-2.177854991012886
5	22	-0.8297293106503609
5	23	-4.538744272296899
5	24	-5.735338662312344
5	25	-1.4986000354421378
5	26	-2.8860063669274236
5	27	-3.340254360649096
5	28	-4.672339307865629
5	29	-0.5786245917824857
5	30	-0.6414334447229209
5	31	1.1389509126351243
5	32	1.3143796992481178
5	33	-2.690230627072733
5	34	2.3065561631351112
5	35	2.720340940735678
5	36	6.303903382192856
5	37	5.554745133034606
5	38	5.212093048277257
5	39	3.3152379053694823
5	40	1.1708304853041689
6	1	0.458352003746743
6	2	-1.362556011240219
6	3	1.9249041163514882
6	4	-3.689994557099814
6	5	-0.3016167312220013
6	6	4.190170502012608
6	7	2.254268562821199
6	8	1.0214567352725297
6	9	3.710337712969295
6	10	-1.8871436799068402
6	11	1.5820773524720906
6	12	9.841389774942407
6	13	3.853004354320145
6	14	5.289625895546951
6	15	6.30151894888737
6	16	7.889718298270928
6	17	11.908799777220825
6	18	11.602893914078123
6	19	14.358598832940938
6	20	7.7766604010025056
6	21	11.199922786764894
6	22	18.39249291157186
6	23	18.10570017214755
6	24	16.842439115465435
6	25	20.67917774233564
6	26	9.980660299739242
6	27	7.126412306017571
6	28	10.972105136578815
6	29	8.28804207488418
6	30	7.403010999721525
6	31	11.227839801524013
6	32	11.02549081035923
6	33	7.598658261816155
6	34	6.706556163135112
6	35	0.12034094073567836
6	36	1.3927922710817446
6	37	1.0214117997012728
6	38	0.9454263816105914
6	39	1.2485712387028158
6	40	1.0374971519708343
7	1	5.813907559302301
7	2	9.059666210982002
7	3	4.169348560795932
7	4	8.465560998455743
7	5	9.898383268777998
7	6	9.990170502012608
7	7	4.920935229487867
7	8	3.8214567352725304
7	9	6.621448824080407
7	10	8.846189653426492
7	11	6.204299574694311
7	12	5.1969453304979645
7	13	6.208559909875703
7	14	7.267403673324729
7	15	12.145963393331812
7	16	7.91194052049315
7	17	11.619910888331937
7	18	0.42511613630034617
7	19	2.669709944052048
7	20	1.9099937343358384
7	21	1.0443672312093373
7	22	3.170270689349639
7	23	1.5723668388142134
7	24	3.0424391154654344
7	25	4.501399964557862
7	26	2.7806602997392424
7	27	3.215301194906459
7	28	5.660994025467705
7	29	1.7547087415508464
7	30	1.8030109997215238
7	31	5.0278398015240136
7	32	2.0921574770258946
7	33	1.5319915951494902
7	34	3.9732228298017773
7	35	2.2758964962912334
7	36	6.192792271081745
7	37	6.999189577479051
7	38	7.545426381610591
7	39	4.98190457203615
7	40	5.170830485304169
8	1	8.758352003746744
8	2	11.004110655426448
8	3	10.558237449684821
8	4	1.243338776233518
8	5	1.0650499354446659
8	6	5.490170502012608
8	7	5.920935229487867
8	8	-0.6785432647274696
8	9	2.010337712969296
8	10	-2.153810346573506
8	11	5.148744019138757
8	12	4.9747231082757395
8	13	4.319671020986814
8	14	10.656292562213618
8	15	1.534852282220701
8	16	6.023051631604261
8	17	-2.324533556112508
8	18	5.202893914078125
8	19	5.89193216627427
8	20	4.076660401002506
8	21	9.599922786764893
8	22	8.39249291157186
8	23	7.739033505480878
8	24	7.709105782132102
8	25	4.945844409002309
8	26	12.44732696640591
8	27	11.493078972684238
8	28	13.272105136578816
8	29	13.58804207488418
8	30	15.803010999721524
8	31	13.027839801524014
8	32	13.75882414369256
8	33	8.698658261816156
8	34	6.139889496468445
8	35	10.220340940735678
8	36	8.859458937748414
8	37	6.888078466367938
8	38	5.045426381610591
8	39	15.315237905369484
8	40	15.504163818637503
9	1	3.3916853370800766
9	2	5.0374439887597795
9	3	0.7249041163514889
9	4	4.043338776233519
9	5	1.431716602111333
9	6	1.1901705020126077
9	7	5.720935229487864
9	8	3.9547900686058632
9	9	4.643671046302629
9	10	4.179522986759828
9	11	3.448744019138754
9	12	4.908056441609073
9	13	2.7863376876534787
9	14	4.956292562213616
9	15	2.3681856155540366
9	16	9.356384964937597
9	17	7.642133110554159
9	18	7.0028939140781254
9	19	2.7585988329409368
9	20	8.443327067669173
9	21	9.533256120098226
9	22	6.59249291157186
9	23	8.239033505480878
9	24	8.909105782132102
9	25	6.812511075668976
9	26	-0.9526730335940901
9	27	-2.2069210273157633
9	28	-0.22789486342118437
9	29	-1.3786245917824864
9	30	-1.4636556669451437
9	31	-0.23882686514265394
9	32	-0.6411758563074379
9	33	-1.1346750715171767
9	34	-1.6267771701982223
9	35	-1.5463257259309886
9	36	0.9261256044150779
9	37	1.4214117997012714
9	38	-0.32124028505607516
9	39	0.31523790536948315
9	40	1.1041638186375025
10	1	7.841685337080076
10	2	8.83744398875978
10	3	11.058237449684821
10	4	10.243338776233518
10	5	9.148383268777998
10	6	16.57350383534594
10	7	14.670935229487863
10	8	12.654790068605863
10	9	14.593671046302632
10	10	11.76285632009316
10	11	11.898744019138757
10	12	10.641389774942407
10	13	11.653004354320146
10	14	13.906292562213618
10	15	10.451518948887369
10	16	8.356384964937597
10	17	11.675466443887494
10	18	9.702893914078125
10	19	13.225265499607605
10	20	7.826660401002506
10	21	10.26658945343156
10	22	11.975826244905193
10	23	12.155700172147546
10	24	8.959105782132102
10	25	14.445844409002309
10	26	5.863993633072575
10	27	4.743078972684238
10	28	6.4387718032454835
10	29	9.83804207488418
10	30	5.219677666388192
10	31	8.777839801524014
10	32	5.5088241436925625
10	33	5.948658261816156
10	34	2.4732228298017773
10	35	3.303674274069012
10	36	3.4427922710817453
10	37	2.138078466367938
10	38	1.045426381610591
10	39	-1.1014287612971838
10	40	-0.41250284802916504
11	1	6.058352003746744
11	2	6.237443988759779
11	3	5.1249041163514875
11	4	2.410005442900186
11	5	3.5983832687779973
11	6	4.490170502012608
11	7	8.654268562821198
11	8	10.121456735272531
11	9	7.110337712969297
11	10	9.912856320093159
11	11	11.882077352472088
11	12	7.941389774942408
11	13	10.253004354320147
11	14	7.68962589554695
11	15	11.50151894888737
11	16	14.78971829827093
11	17	14.608799777220828
11	18	12.502893914078125
11	19	13.458598832940936
11	20	17.576660401002506
11	21	11.699922786764894
11	22	12.59249291157186
11	23	13.605700172147545
11	24	14.742439115465434
11	25	9.379177742335642
11	26	8.680660299739241
11	27	5.526412306017569
11	28	7.672105136578814
11	29	3.18804207488418
11	30	4.403010999721523
11	31	1.2278398015240128
11	32	2.825490810359229
11	33	1.0986582618161567
11	34	1.3065561631351112
11	35	-1.1796590592643224
11	36	-3.8072077289182547
11	37	-3.778588200298728
11	38	-3.454573618389409
11	39	-3.351428761297184
11	40	-3.162502848029165
12	1	0.5916853370800759
12	2	3.504110655426448
12	3	4.5026818941292674
12	4	2.5766721095668537
12	5	3.898383268777998
12	6	3.5457260575681655
12	7	0.14315745171008842
12	8	2.7103456241614197
12	9	0.9547821574137387
12	10	6.51285632009316
12	11	3.5376329080276463
12	12	5.308056441609075
12	13	2.4307821320979244
12	14	4.600737006658061
12	15	9.257074504442926
12	16	11.134162742715372
12	17	12.508799777220826
12	18	10.647338358522568
12	19	15.447487721829827
12	20	13.687771512113617
12	21	16.044367231209336
12	22	12.725826244905193
12	23	8.683477949925324
12	24	8.486883559909877
12	25	8.16806663122453
12	26	6.3362158552948
12	27	0.4375234171286806
12	28	1.327660692134371
12	29	1.865819852661959
12	30	1.0252332219437452
12	31	3.0278398015240136
12	32	2.7588241436925625
12	33	4.865324928482822
12	34	3.8621117186906666
12	35	3.720340940735678
12	36	-0.8072077289182547
12	37	-1.111921533632061
12	38	-1.0101291739449643
12	39	-0.6847620946305168
12	40	-1.3847250702513874
13	1	2.3492610946558337
13	2	2.2919894433052335
13	3	4.4521768436242155
13	4	4.637278170172912
13	5	4.0802014505961814
13	6	0.26289777473988174
13	7	-1.0184587099060742
13	8	-1.7239978101820164
13	9	0.9648831675147491
13	10	3.240129047365887
13	11	1.527531897926636
13	12	-0.35861022505759266
13	13	4.016640717956509
13	14	4.853262259183314
13	15	5.792428039796459
13	16	5.235172843725476
13	17	5.69061795903901
13	18	6.021075732259941
13	19	6.5585988329409375
13	20	9.394842219184323
13	21	8.32719551403762
13	22	8.39249291157186
13	23	7.814791081238456
13	24	7.496984570010888
13	25	10.37008683324473
13	26	4.144296663375606
13	27	5.962775942381207
13	28	5.772105136578816
13	29	4.99713298397509
13	30	4.893920090630614
13	31	0.3005670742512869
13	32	2.5163999012683202
13	33	0.7441128072707013
13	34	2.1247379813169296
13	35	4.629431849826586
13	36	-0.625389547100073
13	37	1.1305027087921804
13	38	-1.0909372547530456
13	39	-0.7150651249335471
13	40	-1.8897755753018926
14	1	-11.241647996253256
14	2	-1.1625560112402198
14	3	-1.2750958836485111
14	4	-4.089994557099814
14	5	-5.601616731222002
14	6	-3.0098294979873916
14	7	1.7542685628211991
14	8	0.3214567352725304
14	9	2.010337712969296
14	10	-7.48714367990684
14	11	-6.017922647527911
14	12	-5.358610225057593
14	13	-6.346995645679854
14	14	-5.510374104453049
14	15	2.7015189488873688
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109	39	1.0930156831472608
109	40	-0.05139173691805432
110	1	-10.141647996253257
110	2	-11.96255601124022
110	3	-11.87509588364851
110	4	-9.489994557099815
110	5	-11.901616731222003
110	6	-8.80982949798739
110	7	-8.7457314371788
110	8	-10.77854326472747
110	9	-8.889662287030704
110	10	-12.88714367990684
110	11	-13.01792264752791
110	12	-11.758610225057593
110	13	-10.946995645679854
110	14	-12.710374104453049
110	15	-12.298481051112631
110	16	-12.110281701729072
110	17	-10.891200222779174
110	18	-9.197106085921876
110	19	-11.241401167059063
110	20	-9.623339598997493
110	21	-9.200077213235108
110	22	-6.6075070884281395
110	23	-8.294299827852456
110	24	-6.157560884534566
110	25	-5.720822257664359
110	26	-5.019339700260757
110	27	-4.67358769398243
110	28	-6.4278948634211845
110	29	-3.5119579251158193
110	30	-3.796989000278476
110	31	-2.372160198475987
110	32	-2.3745091896407713
110	33	0.3986582618161556
110	34	1.106556163135112
110	35	-0.279659059264322
110	36	2.3927922710817446
110	37	2.4214117997012714
110	38	1.3454263816105918
110	39	1.448571238702817
110	40	2.0374971519708343
111	1	-6.027362281967541
111	2	-4.733984582668791
111	3	-5.846524455077082
111	4	-4.089994557099814
111	5	-3.8159024455077155
111	6	-5.581258069415963
111	7	-6.317160008607372
111	8	-3.6071146932988984
111	9	-4.203948001316418
111	10	-2.058572251335411
111	11	-4.160779790385053
111	12	-4.358610225057593
111	13	-4.632709931394141
111	14	-5.367516961595907
111	15	-4.298481051112631
111	16	-5.024567416014785
111	17	-5.205485937064887
111	18	-5.939963228779018
111	19	-5.869972595630491
111	20	-7.423339598997494
111	21	-5.400077213235107
111	22	-3.464649945570997
111	23	-3.951442684995312
111	24	-2.9575608845345656
111	25	-3.577965114807217
111	26	-5.505053985975043
111	27	-0.6735876939824301
111	28	-0.08503772056404202
111	29	-1.3405293536872485
111	30	0.9458681425786661
111	31	-1.5435887699045576
111	32	-1.0030806182122003
111	33	-0.37277030961241486
111	34	-0.2648724082934599
111	35	-0.9939447735500364
111	36	0.6213636996531742
111	37	-1.9214453431558711
111	38	-1.5974307612465521
111	39	-1.494285904154327
111	40	-1.3053599908863083
112	1	-11.408314662919922
112	2	-14.16255601124022
112	3	-9.275095883648511
112	4	-7.75666122376648
112	5	-8.434950064555336
112	6	-6.343162831320725
112	7	-11.7457314371788
112	8	-7.17854326472747
112	9	-10.822995620364036
112	10	-5.48714367990684
112	11	-4.351255980861245
112	12	-9.025276891724259
112	13	-6.346995645679854
112	14	-6.843707437786383
112	15	-6.298481051112631
112	16	-11.643615035062403
112	17	-5.491200222779174
112	18	-5.130439419255209
112	19	-4.4414011670590625
112	20	-8.756672932330826
112	21	-3.4000772132351074
112	22	-2.6075070884281395
112	23	-2.4276331611857884
112	24	-4.2908942178678995
112	25	-1.7208222576643593
112	26	1.1139936330725764
112	27	0.6597456393509038
112	28	1.4387718032454817
112	29	1.7547087415508464
112	30	2.1363443330548577
112	31	-4.3054935318093195
112	32	-3.5745091896407715
112	33	3.1986582618161563
112	34	3.3065561631351112
112	35	3.720340940735678
112	36	5.192792271081745
112	37	2.554745133034606
112	38	2.878759714943925
112	39	2.98190457203615
112	40	3.504163818637501
113	1	-7.57498132958659
113	2	-4.495889344573554
113	3	-3.608429216981845
113	4	-3.423327890433148
113	5	-5.768283397888668
113	6	-5.676496164654058
113	7	-10.579064770512133
113	8	-3.3452099313941357
113	9	-8.989662287030704
113	10	-2.8204770132401737
113	11	-1.0179226475279108
113	12	-4.525276891724259
113	13	-4.51366231234652
113	14	-1.6770407711197155
113	15	1.2015189488873688
113	16	-5.810281701729071
113	17	-6.324533556112508
113	18	-6.963772752588541
113	19	-1.9414011670590625
113	20	-3.9233395989974937
113	21	-1.7334105465684413
113	22	-0.7741737550948056
113	23	-1.5942998278524545
113	24	-1.2908942178678995
113	25	-2.054155590997693
113	26	-0.7193397002607576
113	27	0.993078972684236
113	28	1.4387718032454817
113	29	0.9213754082175143
113	30	2.46967766638819
113	31	-0.8054935318093204
113	32	-0.4078425229741054
113	33	0.3653249284828224
113	34	0.4732228298017773
113	35	-0.44632572593098896
113	36	-1.6405410622515877
113	37	-1.611921533632061
113	38	-1.287906951722742
113	39	-1.1847620946305168
113	40	-0.9958361813624981
115	1	-10.741647996253256
115	2	-4.16255601124022
115	3	-3.275095883648511
115	4	5.910005442900186
115	5	15.898383268777998
115	6	5.990170502012608
115	7	-0.7457314371788009
115	8	-2.1785432647274696
115	9	2.510337712969296
115	10	3.51285632009316
115	11	11.98207735247209
115	12	-5.358610225057593
115	13	6.653004354320146
115	14	-6.510374104453049
115	15	17.70151894888737
115	16	13.689718298270929
115	17	2.508799777220826
115	18	18.202893914078125
115	19	17.558598832940937
115	20	-12.423339598997494
115	21	-10.400077213235107
115	22	-10.60750708842814
115	23	-11.094299827852454
115	24	-9.957560884534566
115	25	-9.72082225766436
115	26	-8.219339700260758
115	27	-8.67358769398243
115	28	-8.227894863421184
115	29	-7.91195792511582
115	30	-7.196989000278476
115	31	-5.9721601984759864
115	32	-5.5745091896407715
115	33	-4.801341738183844
115	34	-4.693443836864889
115	35	-4.279659059264322
115	36	-3.8072077289182547
115	37	-3.778588200298728
115	38	-3.454573618389409
115	39	-3.351428761297184
115	40	-3.162502848029165
116	1	5.591685337080076
116	2	3.504110655426448
116	3	5.724904116351489
116	4	2.243338776233518
116	5	2.898383268777998
116	6	12.656837168679276
116	7	2.920935229487867
116	8	4.488123401939198
116	9	6.510337712969296
116	10	6.846189653426492
116	11	8.648744019138757
116	12	11.308056441609075
116	13	9.319671020986814
116	14	2.8229592288802827
116	15	11.034852282220701
116	16	9.689718298270929
116	17	9.175466443887494
116	18	16.53622724741146
116	19	8.558598832940937
116	20	4.909993734335838
116	21	3.933256120098225
116	22	7.725826244905193
116	23	9.572366838814213
116	24	0.37577244879876837
116	25	0.6125110756689747
116	26	0.11399363307257637
116	27	1.6597456393509038
116	28	2.4387718032454817
116	29	2.7547087415508464
116	30	3.1363443330548577
116	31	4.0278398015240136
116	32	5.092157477025895
116	33	5.53199159514949
116	34	5.639889496468445
116	35	6.053674274069012
116	36	6.526125604415079
116	37	2.888078466367938
116	38	6.878759714943925
116	39	6.98190457203615
116	40	7.170830485304169
118	1	12.258352003746744
118	2	-1.1625560112402198
118	3	11.724904116351489
118	4	-7.089994557099814
118	5	8.898383268777998
118	6	7.990170502012608
118	7	-7.745731437178801
118	8	-9.17854326472747
118	9	8.510337712969296
118	10	13.51285632009316
118	11	-2.017922647527911
118	12	15.641389774942407
118	13	16.653004354320146
118	14	-13.51037410445305
118	15	-13.298481051112631
118	16	-13.310281701729071
118	17	-13.491200222779174
118	18	-13.797106085921875
118	19	-12.441401167059063
118	20	-12.423339598997494
118	21	-10.400077213235107
118	22	-10.60750708842814
118	23	-11.094299827852454
118	24	-9.957560884534566
118	25	-9.72082225766436
118	26	-8.219339700260758
118	27	-8.67358769398243
118	28	-8.227894863421184
118	29	-7.91195792511582
118	30	-7.196989000278476
118	31	-5.9721601984759864
118	32	-5.5745091896407715
118	33	-4.801341738183844
118	34	-4.693443836864889
118	35	-4.279659059264322
118	36	-3.8072077289182547
118	37	-3.778588200298728
118	38	-3.454573618389409
118	39	-3.351428761297184
118	40	-3.162502848029165
119	1	5.258352003746744
119	2	2.83744398875978
119	3	3.724904116351489
119	4	6.910005442900186
119	5	-4.101616731222002
119	6	3.9901705020126084
119	7	6.254268562821199
119	8	3.8214567352725304
119	9	3.510337712969296
119	10	9.51285632009316
119	11	-4.017922647527911
119	12	5.641389774942407
119	13	-7.346995645679854
119	14	4.489625895546951
119	15	10.701518948887369
119	16	4.689718298270929
119	17	4.508799777220826
119	18	10.202893914078125
119	19	16.558598832940937
119	20	16.576660401002506
119	21	12.599922786764893
119	22	3.3924929115718605
119	23	-2.0942998278524545
119	24	3.0424391154654344
119	25	1.2791777423356407
119	26	14.780660299739242
119	27	7.32641230601757
119	28	18.772105136578816
119	29	-7.91195792511582
119	30	-7.196989000278476
119	31	-5.9721601984759864
119	32	-5.5745091896407715
119	33	-4.801341738183844
119	34	-4.693443836864889
119	35	-4.279659059264322
119	36	-3.8072077289182547
119	37	-3.778588200298728
119	38	-3.454573618389409
119	39	-3.351428761297184
119	40	-3.162502848029165
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1288.0
5	106.0
6	78.0
7	86.0
8	56.0
9	79.0
10	118.0
11	148.0
12	148.0
13	199.0
14	235.0
15	229.0
16	264.0
17	278.0
18	302.0
19	256.0
20	291.0
21	294.0
22	306.0
23	280.0
24	229.0
25	196.0
26	180.0
27	132.0
28	85.0
29	74.0
30	48.0
31	22.0
32	10.0
33	7.0
34	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	98.9202657807309	0.7308970099667774	0.18272425249169436	0.16611295681063123
8	97.8253652058433	0.9462151394422311	0.5146082337317397	0.7138114209827356
9	94.96928440976258	2.673086501743317	1.3614477834965963	0.9961813049975096
10	48.19117158977763	32.47593760371723	8.148025224029206	11.184865582475938
11	34.002655160969134	35.11450381679389	17.723199468967806	13.159641553269166
12	38.94789246598075	13.707268503153003	17.490872884168603	29.85396614669764
13	26.236309326252904	25.174244938599404	23.46498506471955	25.124460670428146
14	21.1583139727846	21.224692997012944	27.779621639561896	29.83737139064056
15	34.79920345170926	18.702290076335878	26.58479920345171	19.913707268503153
16	30.219050779953534	18.586126783936276	24.178559575174244	27.016262860935946
17	28.393627613674077	26.734152007965484	22.535678725522736	22.336541652837703
18	36.19316296050448	20.760039827414538	21.871888483239296	21.174908728841686
19	30.228295284119312	16.11398100316614	18.096983836027327	35.56073987668722
20	32.40955857948889	25.572519083969464	21.257882509127114	20.760039827414538
21	22.768005310321936	28.80849651510123	25.058081646199803	23.365416528377033
22	21.058745436442084	18.40358446730833	32.44274809160305	28.094922004646534
23	35.518672199170126	19.08713692946058	26.190871369294605	19.203319502074688
24	27.115831397278463	26.21971457019582	24.228343843345503	22.43611018918022
25	33.30564784053156	18.222591362126245	24.95016611295681	23.521594684385384
26	17.577276524644947	25.59732664995823	29.82456140350877	27.000835421888052
27	16.544971788914705	16.163292399601726	39.81081978094922	27.480916030534353
28	25.680610889774236	18.459495351925632	29.515272244355913	26.344621513944222
29	27.958776595744684	17.486702127659576	31.466090425531917	23.08843085106383
30	23.436460412508318	16.949434464404526	33.749168330006654	25.864936793080506
31	22.255489021956087	16.500332667997338	37.69128409846973	23.55289421157685
32	21.975103734439834	17.029045643153527	37.294605809128626	23.70124481327801
33	19.980086292731496	20.096249585131098	39.478924659807504	20.444739462329903
34	13.97278460006638	22.369731164951876	44.95519415864587	18.702290076335878
35	12.296714238300698	26.50182542316628	41.08861599734484	20.112844341188186
36	13.10985728509791	28.410222369731166	38.5330235645536	19.946896780617323
37	15.764119601328904	26.843853820598007	38.72093023255814	18.67109634551495
38	19.515433123133093	25.091271158313972	36.59143710587454	18.801858612678394
39	16.959840690341853	24.991702621971456	37.02290076335878	21.025555924327914
40	14.83571191503485	24.875539329571854	38.01858612678394	22.27016262860936
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0
17	4.0
18	4.0
19	5.5
20	7.0
21	12.5
22	18.0
23	18.0
24	30.0
25	42.0
26	55.0
27	68.0
28	68.0
29	80.5
30	93.0
31	101.0
32	109.0
33	109.0
34	142.0
35	175.0
36	185.0
37	195.0
38	195.0
39	233.5
40	272.0
41	292.0
42	312.0
43	312.0
44	330.0
45	348.0
46	336.5
47	325.0
48	325.0
49	346.5
50	368.0
51	360.0
52	352.0
53	352.0
54	370.5
55	389.0
56	405.5
57	422.0
58	422.0
59	409.5
60	397.0
61	428.0
62	459.0
63	459.0
64	467.0
65	475.0
66	459.5
67	444.0
68	444.0
69	385.5
70	327.0
71	270.0
72	213.0
73	213.0
74	162.0
75	111.0
76	85.0
77	59.0
78	59.0
79	43.0
80	27.0
81	19.5
82	12.0
83	12.0
84	6.5
85	1.0
86	1.5
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.09956853634251576
8	0.033189512114171926
9	0.04978426817125788
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.414868901427149
20	0.0
21	0.0
22	0.0
23	0.016594756057085963
24	0.0
25	0.09956853634251576
26	0.6803849983405245
27	0.0
28	0.033189512114171926
29	0.1659475605708596
30	0.23232658479920343
31	0.23232658479920343
32	0.016594756057085963
33	0.0
34	0.0
35	0.0
36	0.0
37	0.09956853634251576
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	6026.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.24228343843346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.66715704303053	85.42980418187852
2	3.5490989334314085	6.405575838035181
3	0.8826774549466716	2.3896448722203782
4	0.3493931592497242	1.261201460338533
5	0.12872379551305627	0.5808164619980086
6	0.0735564545788893	0.39827414537006306
7	0.0735564545788893	0.46465316959840686
8	0.12872379551305627	0.9293063391968137
9	0.018389113644722326	0.14935280451377364
>10	0.12872379551305627	1.9913707268503154
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	27	0.4480584135413209	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	22	0.36508463325589113	No Hit
AGGGTCGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC	18	0.2987056090275473	No Hit
AGGGTCGGGGACACGCAGGGATGAGATGGGGAAAAAAAAA	15	0.24892134085628942	No Hit
AGGGTCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAA	14	0.23232658479920343	No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC	12	0.19913707268503153	No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	12	0.19913707268503153	No Hit
AGGGTCGGGGACACGCAGGGATGAGATGGCCCAAAAAAAA	9	0.14935280451377364	No Hit
AGGGTCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAA	8	0.1327580484566877	No Hit
AGGGTCGGGGAGTGCAGTGCTCGGGTCGGGGACACGCAGG	8	0.1327580484566877	No Hit
AGGGTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	8	0.1327580484566877	No Hit
AGGGTCGGGAACCAGTCTCTTGTCTCAGGCTCTCTGCCTT	8	0.1327580484566877	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG	8	0.1327580484566877	No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC	8	0.1327580484566877	No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC	8	0.1327580484566877	No Hit
AGGGTCGGGAGTGCAGTGCTAGGGTCGGGGACACGCAGGG	7	0.11616329239960171	No Hit
AGGGTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	7	0.11616329239960171	No Hit
AGGGTCGGGGGGGCTGGTGAGCTGGCTCAGTGGGTAAGAG	7	0.11616329239960171	No Hit
AGGGTCGGGCTCGTCTTAATCAGCAACATCTTTTGATACA	7	0.11616329239960171	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATGAG	45	4.2000465E-9	33.966667	19
ACACGCA	35	8.7804256E-7	33.966667	11
CGGGGGT	25	1.8089853E-4	33.966667	6
CGGGGGC	20	0.0025871173	33.966667	6
CGGGGCT	20	0.0025871173	33.966667	6
GCAGGGA	35	8.7804256E-7	33.966667	15
GGACACG	40	6.0865204E-8	33.966667	9
AGGGATG	35	8.7804256E-7	33.966667	17
GATGAGA	45	4.2000465E-9	33.966667	20
GACACGC	45	4.2000465E-9	33.966667	10
CAGGGAT	35	8.7804256E-7	33.966667	16
CGGGAGT	25	1.8089853E-4	33.966667	6
CGGGAGC	20	0.0025871173	33.966667	6
AGATGGG	35	8.7804256E-7	33.966667	24
GGAAGCA	20	0.0025871173	33.966667	8
GGGATGA	45	4.2000465E-9	33.966667	18
GGGAAGT	25	1.8089853E-4	33.966667	7
GGGAAGC	25	1.8089853E-4	33.966667	7
GTCGGGT	50	2.8921932E-10	33.966667	4
ATGAGAT	45	4.2000465E-9	33.966667	21
>>END_MODULE