FastQCFastQC Report
Fri 17 Jun 2016
SRR1525823_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525823_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8043
Sequences flagged as poor quality0
Sequence length40
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT1271.5790128061668531No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC1031.2806166853164243No Hit
AGGGTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG360.4475941812756434No Hit
AGGGTCGGGACTAATCCTAGCCCTAGCCCTACACAAATAT320.39786149446723856No Hit
AGGGTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT310.3854283227651374No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG280.34812880765883375No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA230.28596294914832776No Hit
AGGGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA200.24866343404202412No Hit
AGGGTCGGGGTTGTGTGGCTTTGGCCCTAAGCCATTGTCT160.19893074723361928No Hit
AGGGTCGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGT150.18649757553151808No Hit
AGGGTCGGGGTATGTGTGTCTGTTTGTCTGGTATGATGTG120.14919806042521447No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG120.14919806042521447No Hit
AGGGTCGGGATGCCACAACTAGATACATCAACATGATTTA110.13676488872311326No Hit
AGGGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.13676488872311326No Hit
AGGGTCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC100.12433171702101206No Hit
AGGGTCGGGATAGCAGGATTTGTTCACTGATTCCCATTAT100.12433171702101206No Hit
AGGGTCGGGGGGGCTGGGGAGATGGCTCAGTGGTTAAGAG90.11189854531891084No Hit
AGGGTCGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG90.11189854531891084No Hit
AGGGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.11189854531891084No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAG90.11189854531891084No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG90.11189854531891084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTCG7950.034.0000041
ATCCTCT251.8543858E-434.016
TCCTCTT251.8543858E-434.017
GGGTCGG7850.034.02
GGGTCAC359.229316E-734.07
GTCACAG251.8543858E-434.09
ACAGATC200.002627475734.012
CGGGGGT503.2014214E-1034.06
CGGGGGG552.1827873E-1134.06
CGGGGGC251.8543858E-434.06
CGGGGGA301.30865465E-534.06
CGGGGCT200.002627475734.06
CGGGGCC301.30865465E-534.06
CGGGGAT200.002627475734.06
CGGGGAG251.8543858E-434.06
ATTGGGC200.002627475734.033
GGTCACA301.30865465E-534.08
GGGAGTT251.8543858E-434.07
GATTGGG200.002627475734.032
CGGGCTC200.002627475734.06
Produced by FastQC (version 0.11.5)