##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525823_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8043
Sequences flagged as poor quality	0
Sequence length	40
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.92378465746612	30.0	4.0	33.0	4.0	35.0
2	14.356210369265199	11.0	4.0	24.0	4.0	33.0
3	13.92490364291931	11.0	4.0	23.0	4.0	30.0
4	13.67213726221559	9.0	4.0	23.0	4.0	33.0
5	12.99278876041278	9.0	4.0	21.0	4.0	30.0
6	15.819967673753574	13.0	4.0	28.0	4.0	33.0
7	16.518960586845704	15.0	4.0	30.0	4.0	33.0
8	17.01566579634465	16.0	4.0	30.0	4.0	33.0
9	16.732438144970782	11.0	4.0	31.0	4.0	34.0
10	14.972274027104314	4.0	4.0	28.0	4.0	33.0
11	14.425463135645904	4.0	4.0	28.0	4.0	34.0
12	14.01230883998508	4.0	4.0	26.0	4.0	33.0
13	13.12893199055079	4.0	4.0	24.0	4.0	32.0
14	13.45455675742882	4.0	4.0	26.0	4.0	33.0
15	13.424841477060799	4.0	4.0	26.0	4.0	33.0
16	11.258361307969663	4.0	4.0	20.0	4.0	30.0
17	10.656844461022006	4.0	4.0	18.0	4.0	28.0
18	10.341912221807783	4.0	4.0	17.0	4.0	28.0
19	10.187243565833644	4.0	4.0	15.0	4.0	28.0
20	10.742260350615442	4.0	4.0	19.0	4.0	30.0
21	9.413775954245928	4.0	4.0	13.0	4.0	26.0
22	9.15603630486137	4.0	4.0	12.0	4.0	25.0
23	9.251647395250528	4.0	4.0	12.0	4.0	26.0
24	8.86472709188114	4.0	4.0	11.0	4.0	24.0
25	9.477558125077707	4.0	4.0	14.0	4.0	26.0
26	9.623026233992292	4.0	4.0	14.0	4.0	27.0
27	9.82680591818973	4.0	4.0	14.0	4.0	28.0
28	10.158895934352854	4.0	4.0	16.0	4.0	29.0
29	9.140743503667785	4.0	4.0	12.0	4.0	26.0
30	9.518587591694642	4.0	4.0	14.0	4.0	27.0
31	10.658833768494343	4.0	4.0	18.0	4.0	31.0
32	10.448091508143728	4.0	4.0	17.0	4.0	31.0
33	10.343404202412035	4.0	4.0	16.0	4.0	31.0
34	10.543578266815866	4.0	4.0	17.0	4.0	32.0
35	10.504662439388287	4.0	4.0	16.0	4.0	32.0
36	10.51721994280741	4.0	4.0	13.0	4.0	33.0
37	10.384060673877906	4.0	4.0	11.0	4.0	33.0
38	10.186000248663435	4.0	4.0	4.0	4.0	32.0
39	9.907372870819346	4.0	4.0	4.0	4.0	32.0
40	10.086907870197688	4.0	4.0	4.0	4.0	32.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	10.712256114198848
1	2	16.103642953194047
1	3	1.0079175588773097
1	4	14.433975505918234
1	5	22.396361924968733
1	6	-0.7545107455324196
1	7	-11.349957669609369
1	8	-11.752166123350339
1	9	-11.23450475565027
1	10	-9.387536782962483
1	11	-9.238899883226507
1	12	-9.10622359150997
1	13	-8.124427831370555
1	14	-8.502041629680951
1	15	-8.727419106521268
1	16	-6.40689368509028
1	17	-5.928918381240363
1	18	-5.883849175420387
1	19	-5.646736742789374
1	20	-6.217894583916255
1	21	-4.394395198884363
1	22	-4.2216197184308655
1	23	-4.0278480875849265
1	24	-3.9109346389764355
1	25	-4.202557230351349
1	26	-4.422938722783924
1	27	-4.2687909553466845
1	28	-4.704085390014182
1	29	-3.7733308341125715
1	30	-3.970559980954717
1	31	-5.054736210641789
1	32	-5.041545442861231
1	33	-5.03242847537739
1	34	-5.397681425042105
1	35	-5.096415690432721
1	36	-5.097489573549947
1	37	-4.9689375132950975
1	38	-5.02866393410511
1	39	-4.826742710334042
1	40	-4.886547930054121
2	1	3.8789227808655156
2	2	-4.313023713472623
2	3	2.0079175588773097
2	4	-1.816024494081768
2	5	0.3130285916353994
2	6	1.0788225878009126
2	7	4.816708997057299
2	8	1.3311672099829934
2	9	-9.23450475565027
2	10	-2.637536782962483
2	11	-5.57223321655984
2	12	-0.35622359150996985
2	13	0.9589055019627786
2	14	0.41462503698571496
2	15	0.9392475601453985
2	16	-3.40689368509028
2	17	-0.1789183812403632
2	18	-0.050515842087053286
2	19	1.5199299238772923
2	20	1.7821054160837448
2	21	5.0222714677823035
2	22	-0.8049530517641985
2	23	5.805485245748407
2	24	2.0890653610235645
2	25	5.630776102981985
2	26	5.577061277216076
2	27	2.8978757113199816
2	28	3.462581276652484
2	29	2.2266691658874285
2	30	1.696106685711949
2	31	6.028597122691545
2	32	5.0417878904721025
2	33	5.550904857955944
2	34	6.102318574957895
2	35	5.236917642900613
2	36	7.235843759783386
2	37	7.114395820038235
2	38	5.304669399228224
2	39	3.4232572896659583
2	40	4.780118736612545
3	1	-3.9847135827708478
3	2	-4.653932804381714
3	3	-2.749658198698448
3	4	-1.8690547971120708
3	5	-2.7854562568494483
3	6	0.2757922847706098
3	7	-6.077230396882095
3	8	-2.3885297597139754
3	9	-0.9617774829229973
3	10	-5.387536782962483
3	11	0.03382738950076636
3	12	-0.8334963187826965
3	13	-1.760791467734192
3	14	-4.956587084226405
3	15	-1.4637924854099538E-4
3	16	2.229469951273357
3	17	3.525627073305092
3	18	-1.7020309936022056
3	19	0.6259905299378996
3	20	1.6911963251746531
3	21	1.4237866192974558
3	22	1.9601984633873162
3	23	3.153970094233255
3	24	3.1799744519326563
3	25	2.161079133285014
3	26	5.577061277216076
3	27	6.367572681016952
3	28	5.841369155440363
3	29	3.2266691658874285
3	30	5.756712746318009
3	31	6.945263789358211
3	32	6.685727284411495
3	33	7.240298797349883
3	34	5.329591302230622
3	35	7.812675218658189
3	36	5.266146790086417
3	37	3.212880668523084
3	38	4.698608793167617
3	39	4.445984562393232
3	40	1.658906615400424
4	1	-0.6210772191344844
4	2	24.436976286527376
4	3	15.341250892210644
4	4	25.767308839251566
4	5	19.396361924968733
4	6	22.912155921134246
4	7	20.650042330390633
4	8	21.24783387664966
4	9	21.76549524434973
4	10	13.612463217037517
4	11	22.76110011677349
4	12	18.89377640849003
4	13	19.875572168629446
4	14	16.497958370319047
4	15	25.272580893478732
4	16	-6.40689368509028
4	17	-5.928918381240363
4	18	-5.883849175420387
4	19	-5.646736742789374
4	20	-6.217894583916255
4	21	-4.394395198884363
4	22	-4.2216197184308655
4	23	-4.0278480875849265
4	24	-3.9109346389764355
4	25	-4.202557230351349
4	26	-4.422938722783924
4	27	-4.2687909553466845
4	28	-4.704085390014182
4	29	-3.7733308341125715
4	30	-3.970559980954717
4	31	-5.054736210641789
4	32	-5.041545442861231
4	33	-5.03242847537739
4	34	-5.397681425042105
4	35	-5.096415690432721
4	36	-5.097489573549947
4	37	-4.9689375132950975
4	38	-5.02866393410511
4	39	-4.826742710334042
4	40	-4.886547930054121
5	1	12.96715807498316
5	2	18.672270404174434
5	3	19.576545009857703
5	4	20.237897074545682
5	5	11.043420748498146
5	6	5.500391215251895
5	7	-5.467604728432898
5	8	-3.163930829232692
5	9	-0.5286224027090949
5	10	1.906580864096341
5	11	-0.7683116479323893
5	12	-3.1650471209217343
5	13	-4.065604301958791
5	14	-0.6785122179162446
5	15	-4.433301459462443
5	16	-0.4657172145020443
5	17	-0.04656544006389218
5	18	-0.589731528361563
5	19	-2.4702661545540794
5	20	-2.1590710545044907
5	21	-0.04145402241377383
5	22	-0.22161971843086548
5	23	-1.3807892640555144
5	24	-2.734464050741141
5	25	-2.555498406821937
5	26	-2.4229387227839236
5	27	-2.7393791906408023
5	28	-2.4099677429553585
5	29	-2.714507304700807
5	30	-1.6764423338958938
5	31	-2.1723832694653176
5	32	-2.982721913449467
5	33	-4.267722593024449
5	34	-2.3388578956303405
5	35	-3.21406274925625
5	36	-2.5092542794323
5	37	-2.0865845721186265
5	38	-1.6168992282227563
5	39	-3.7090956515105127
5	40	-1.4747832241717678
6	1	12.656700558643294
6	2	12.38142073097182
6	3	4.452362003321754
6	4	4.267308839251566
6	5	10.118584147190955
6	6	14.023267032245357
6	7	6.372264552612853
6	8	13.247833876649661
6	9	13.654384133238619
6	10	3.501352105926406
6	11	7.0388778945512716
6	12	15.171554186267809
6	13	10.597794390851666
6	14	15.72018059254127
6	15	14.4392475601454
6	16	16.31532853713194
6	17	14.848859396537415
6	18	11.560595269024056
6	19	12.297707701655069
6	20	15.893216527194856
6	21	11.661160356671195
6	22	12.500602503791356
6	23	14.805485245748406
6	24	10.144620916579122
6	25	9.964109436315319
6	26	14.577061277216076
6	27	13.508986822431094
6	28	15.740359054430261
6	29	13.004446943665208
6	30	14.69610668571195
6	31	16.778597122691544
6	32	16.014010112694326
6	33	14.634238191289278
6	34	15.935651908291227
6	35	15.348028754011722
6	36	6.0691770931167195
6	37	8.919951375593792
6	38	7.0268916214504475
6	39	6.617701734110403
6	40	9.613452069945879
7	1	9.628922780865516
7	2	15.186976286527377
7	3	12.278750892210644
7	4	16.642308839251566
7	5	10.146361924968733
7	6	10.224655921134246
7	7	2.1500423303906313
7	8	8.685333876649661
7	9	9.64049524434973
7	10	10.112463217037517
7	11	0.573600116773493
7	12	8.95627640849003
7	13	5.188072168629445
7	14	2.060458370319049
7	15	4.460080893478732
7	16	4.59310631490972
7	17	6.446081618759637
7	18	4.366150824579613
7	19	4.478263257210626
7	20	6.532105416083745
7	21	10.855604801115637
7	22	7.6533802815691345
7	23	9.409651912415073
7	24	5.7765653610235645
7	25	10.35994276964865
7	26	8.014561277216076
7	27	6.2937090446533155
7	28	6.608414609985818
7	29	5.3516691658874285
7	30	6.404440019045283
7	31	8.382763789358211
7	32	8.083454557138769
7	33	6.78007152462261
7	34	6.664818574957895
7	35	8.466084309567279
7	36	3.8400104264500534
7	37	3.5310624867049025
7	38	1.5963360658948904
7	39	3.2357572896659583
7	40	2.613452069945879
8	1	9.807494209436943
8	2	-1.848737999186909
8	3	4.555536606496359
8	4	10.767308839251566
8	5	13.182076210683018
8	6	7.055013063991389
8	7	11.864328044676347
8	8	11.533548162363946
8	9	12.408352387206872
8	10	5.68389178846609
8	11	0.261100116773493
8	12	1.6080621227757437
8	13	2.161286454343731
8	14	3.4265297988904777
8	15	1.3440094649073036
8	16	3.8788206006240067
8	17	2.2853673330453503
8	18	2.4732936817224704
8	19	0.9961204000677686
8	20	0.28210541608374484
8	21	-0.037252341741504935
8	22	0.7783802815691345
8	23	0.4007233409865023
8	24	-3.196648924690721
8	25	-1.059700087494206
8	26	2.0770612772160764
8	27	1.2312090446533155
8	28	2.3673431814143893
8	29	-0.1304736912554283
8	30	1.0294400190452828
8	31	1.230978075072498
8	32	4.744168842853055
8	33	3.110428667479752
8	34	0.5308900035293238
8	35	3.97501288099585
8	36	5.045367569307196
8	37	4.816776772419189
8	38	4.399907494466319
8	39	5.530400146808816
8	40	4.18488064137445
9	1	-4.121077219134484
9	2	-4.813023713472623
9	3	-0.6587491077893564
9	4	-1.482691160748434
9	5	-4.353638075031267
9	6	-4.3378440788657535
9	7	-1.3499576696093687
9	8	1.7478338766496613
9	9	0.5154952443497294
9	10	4.112463217037517
9	11	4.261100116773493
9	12	-3.10622359150997
9	13	4.625572168629445
9	14	2.997958370319049
9	15	3.0225808934787324
9	16	3.34310631490972
9	17	-2.928918381240363
9	18	4.116150824579613
9	19	1.8532632572106262
9	20	6.032105416083745
9	21	3.3556048011156374
9	22	6.0283802815691345
9	23	5.4721519124150735
9	24	2.5890653610235645
9	25	5.047442769648651
9	26	6.827061277216076
9	27	5.7312090446533155
9	28	7.045914609985818
9	29	1.7266691658874285
9	30	0.7794400190452828
9	31	6.695263789358211
9	32	2.9584545571387686
9	33	7.46757152462261
9	34	2.852318574957895
9	35	3.153584309567279
9	36	4.652510426450053
9	37	5.2810624867049025
9	38	7.22133606589489
9	39	8.423257289665958
9	40	5.613452069945879
10	1	6.312256114198849
10	2	5.503642953194044
10	3	6.607917558877309
10	4	7.100642172584898
10	5	-1.2703047416979327
10	6	14.04548925446758
10	7	12.983375663723963
10	8	13.114500543316328
10	9	13.032161911016395
10	10	13.879129883704183
10	11	13.227766783440158
10	12	17.29377640849003
10	13	15.542238835296112
10	14	12.631291703652382
10	15	17.805914226812067
10	16	7.926439648243052
10	17	8.404414952092969
10	18	10.98281749124628
10	19	10.219929923877293
10	20	11.982105416083744
10	21	10.73893813444897
10	22	7.978380281569134
10	23	9.172151912415073
10	24	9.6223986943569
10	25	10.730776102981984
10	26	11.777061277216076
10	27	14.664542377986649
10	28	10.62924794331915
10	29	8.160002499220763
10	30	11.296106685711948
10	31	11.811930456024879
10	32	12.425121223805434
10	33	14.234238191289275
10	34	13.935651908291227
10	35	11.236917642900611
10	36	12.16917709311672
10	37	12.231062486704902
10	38	12.104669399228223
10	39	13.706590622999293
10	40	13.71345206994588
11	1	-0.6210772191344844
11	2	6.936976286527377
11	3	-0.15874910778935636
11	4	3.9339755059182338
11	5	1.8963619249687333
11	6	9.578822587800914
11	7	7.316708997057299
11	8	7.414500543316329
11	9	7.932161911016397
11	10	9.945796550370849
11	11	3.427766783440161
11	12	2.39377640849003
11	13	4.208905501962777
11	14	-0.5020416296809511
11	15	1.4392475601453985
11	16	1.4264396482430541
11	17	1.9044149520929707
11	18	-0.21718250875372114
11	19	0.6865965905439602
11	20	2.1154387494170788
11	21	1.2722714677823035
11	22	1.4450469482358006
11	23	6.13881857908174
11	24	2.0890653610235645
11	25	1.6307761029819847
11	26	3.7437279438827424
11	27	4.2312090446533155
11	28	5.462581276652484
11	29	3.2266691658874285
11	30	8.69610668571195
11	31	7.778597122691544
11	32	7.291787890472101
11	33	2.4675715246226098
11	34	6.768985241624563
11	35	7.070250976233947
11	36	7.235843759783386
11	37	9.19772915337157
11	38	5.804669399228224
11	39	8.673257289665958
11	40	8.446785403279211
12	1	7.795589447532183
12	2	9.77030961986071
12	3	8.007917558877311
12	4	0.933975505918232
12	5	5.7296952583020655
12	6	13.828822587800914
12	7	11.983375663723963
12	8	10.081167209982993
12	9	10.348828577683062
12	10	8.529129883704185
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116	21	-4.394395198884363
116	22	-4.2216197184308655
116	23	-4.0278480875849265
116	24	-3.9109346389764355
116	25	-4.202557230351349
116	26	-4.422938722783924
116	27	-4.2687909553466845
116	28	-4.704085390014182
116	29	-3.7733308341125715
116	30	-3.970559980954717
116	31	-5.054736210641789
116	32	-5.041545442861231
116	33	-5.03242847537739
116	34	-5.397681425042105
116	35	-5.096415690432721
116	36	-5.097489573549947
116	37	-4.9689375132950975
116	38	-5.02866393410511
116	39	-4.826742710334042
116	40	-4.886547930054121
117	1	2.7122561141988477
117	2	-1.118579269028178
117	3	-2.5476379966782456
117	4	0.5450866170293445
117	5	-2.2703047416979327
117	6	-0.9767329677546428
117	7	-7.572179891831591
117	8	-1.1966105677947834
117	9	-1.790060311205826
117	10	-2.0542034496291492
117	11	-0.016677661004285582
117	12	3.2271097418233623
117	13	-2.6799833869261107
117	14	-3.835374963014285
117	15	-2.282974662076823
117	16	-3.5180047962013905
117	17	-4.373362825684808
117	18	-2.661626953198165
117	19	-4.535625631678263
117	20	-2.995672361694033
117	21	-2.616617421106585
117	22	-2.443841940653088
117	23	-1.5834036431404819
117	24	-0.9109346389764355
117	25	-4.202557230351349
117	26	-4.422938722783924
117	27	-4.2687909553466845
117	28	-4.704085390014182
117	29	-3.7733308341125715
117	30	-3.970559980954717
117	31	-5.054736210641789
117	32	-5.041545442861231
117	33	-5.03242847537739
117	34	-5.397681425042105
117	35	-5.096415690432721
117	36	-5.097489573549947
117	37	-4.9689375132950975
117	38	-5.02866393410511
117	39	-4.826742710334042
117	40	-4.886547930054121
119	1	-1.3710772191344844
119	2	0.4369762865273774
119	3	-3.9087491077893564
119	4	-0.7326911607484341
119	5	-4.853638075031267
119	6	-5.5878440788657535
119	7	0.1500423303906313
119	8	-3.2521661233503387
119	9	-5.234504755650271
119	10	2.112463217037517
119	11	-1.738899883226507
119	12	-4.10622359150997
119	13	-0.6244278313705554
119	14	-8.502041629680951
119	15	-8.727419106521268
119	16	-6.40689368509028
119	17	-5.928918381240363
119	18	-5.883849175420387
119	19	-5.646736742789374
119	20	-6.217894583916255
119	21	-4.394395198884363
119	22	-4.2216197184308655
119	23	-4.0278480875849265
119	24	-3.9109346389764355
119	25	-4.202557230351349
119	26	-4.422938722783924
119	27	-4.2687909553466845
119	28	-4.704085390014182
119	29	-3.7733308341125715
119	30	-3.970559980954717
119	31	-5.054736210641789
119	32	-5.041545442861231
119	33	-5.03242847537739
119	34	-5.397681425042105
119	35	-5.096415690432721
119	36	-5.097489573549947
119	37	-4.9689375132950975
119	38	-5.02866393410511
119	39	-4.826742710334042
119	40	-4.886547930054121
120	1	-6.7877438858011505
120	2	-9.563023713472623
120	3	-8.658749107789356
120	4	-8.232691160748434
120	5	-7.603638075031267
120	6	-10.087844078865754
120	7	-11.349957669609369
120	8	-11.752166123350339
120	9	-11.23450475565027
120	10	-9.387536782962483
120	11	-9.238899883226507
120	12	-9.10622359150997
120	13	-8.124427831370555
120	14	-8.502041629680951
120	15	-8.727419106521268
120	16	-6.40689368509028
120	17	-5.928918381240363
120	18	-5.883849175420387
120	19	-5.646736742789374
120	20	-6.217894583916255
120	21	-4.394395198884363
120	22	-4.2216197184308655
120	23	-4.0278480875849265
120	24	-3.9109346389764355
120	25	-4.202557230351349
120	26	-4.422938722783924
120	27	-4.2687909553466845
120	28	-4.704085390014182
120	29	-3.7733308341125715
120	30	-3.970559980954717
120	31	-5.054736210641789
120	32	-5.041545442861231
120	33	-5.03242847537739
120	34	-5.397681425042105
120	35	-5.096415690432721
120	36	-5.097489573549947
120	37	-4.9689375132950975
120	38	-5.02866393410511
120	39	-4.826742710334042
120	40	-4.886547930054121
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	3512.0
5	54.0
6	90.0
7	175.0
8	231.0
9	254.0
10	293.0
11	247.0
12	209.0
13	189.0
14	153.0
15	108.0
16	76.0
17	80.0
18	60.0
19	99.0
20	190.0
21	246.0
22	308.0
23	309.0
24	294.0
25	251.0
26	211.0
27	158.0
28	107.0
29	68.0
30	41.0
31	21.0
32	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	98.80567305299826	0.5971634735008708	0.18661358546902215	0.4105498880318487
8	98.66832607342874	0.6471686372121966	0.37336652146857496	0.3111387678904792
9	97.75812679038486	0.8718395815170008	0.7348362187071865	0.6351974093909578
10	50.06843349508523	28.729625482145078	10.265024262784621	10.936916759985069
11	34.7058091802463	23.771613384749347	24.169672844881205	17.35290459012315
12	32.156180054712756	20.343198209400644	25.043521512061677	22.45710022382492
13	25.012437810945276	21.082089552238806	27.238805970149254	26.666666666666668
14	20.92502797463633	26.694019644411288	32.05271664801691	20.32823573293547
15	29.416884247171453	24.978241949521323	29.70284719631978	15.902026606987443
16	33.93012557503419	25.102573666542334	25.189605868457043	15.777694889966432
17	28.015419049987567	23.73787615021139	31.720964934095996	16.52573986570505
18	26.45778938207137	25.276638070371753	29.628248166107173	18.63732438144971
19	26.02910085810223	27.359781121751027	26.626041537122248	19.9850764830245
20	25.61233370632848	28.882257863981103	29.528782792490365	15.976625637200051
21	24.692279000372995	26.532388412283975	29.976376973766005	18.79895561357702
22	22.14347880144225	24.44361556633097	31.182394628869826	22.230511003356956
23	22.12686567164179	25.982587064676615	30.2363184079602	21.654228855721396
24	23.175432052716648	25.26420489866965	29.901777943553398	21.6585851050603
25	25.02798855579052	26.84413484264212	26.209727578056974	21.918149023510384
26	25.408099688473516	26.13084112149533	24.847352024922117	23.613707165109034
27	18.99002493765586	28.216957605985037	26.14713216957606	26.645885286783045
28	21.027746671643648	31.143461490605944	25.071544108498195	22.75724772925221
29	27.307884231536928	28.954590818363275	24.35129740518962	19.386227544910177
30	24.96268656716418	26.778606965174127	30.17412935323383	18.084577114427862
31	26.293532338308456	29.440298507462686	25.72139303482587	18.544776119402986
32	28.51805728518057	25.354919053549192	26.861768368617682	19.265255292652554
33	27.471707499067282	27.22298221614227	25.519214028105957	19.786096256684495
34	21.337975627953245	28.537677194727678	31.27331509574733	18.85103208157175
35	18.66219072485391	31.605122466741264	32.86087280865348	16.871813999751335
36	21.00547536087606	33.6112493777999	26.580388252862118	18.802887008461923
37	23.672758920800696	30.349372124829046	27.713539723983587	18.26432923038667
38	26.01019520079572	30.026109660574413	25.63720004973269	18.326495088897175
39	23.613528972892315	29.66923650833126	23.93683163392191	22.780402884854514
40	23.150565709312446	26.70645281611339	28.608728086534875	21.53425338803929
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	1.0
15	1.0
16	9.0
17	17.0
18	17.0
19	24.5
20	32.0
21	41.0
22	50.0
23	50.0
24	42.5
25	35.0
26	41.0
27	47.0
28	47.0
29	69.5
30	92.0
31	94.0
32	96.0
33	96.0
34	118.5
35	141.0
36	179.5
37	218.0
38	218.0
39	254.0
40	290.0
41	354.5
42	419.0
43	419.0
44	455.5
45	492.0
46	540.5
47	589.0
48	589.0
49	655.5
50	722.0
51	707.0
52	692.0
53	692.0
54	777.0
55	862.0
56	822.5
57	783.0
58	783.0
59	733.5
60	684.0
61	680.0
62	676.0
63	676.0
64	647.5
65	619.0
66	441.5
67	264.0
68	264.0
69	198.0
70	132.0
71	88.0
72	44.0
73	44.0
74	40.5
75	37.0
76	20.0
77	3.0
78	3.0
79	3.0
80	3.0
81	2.5
82	2.0
83	2.0
84	1.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.06216585851050603
8	0.09946537361680964
9	0.17406440382941687
10	0.07459903021260723
11	0.04973268680840482
12	0.012433171702101205
13	0.03729951510630362
14	0.0
15	0.0
16	0.0
17	0.012433171702101205
18	0.0
19	0.02486634340420241
20	0.0
21	0.0
22	0.0
23	0.03729951510630362
24	0.0
25	0.04973268680840482
26	0.2237970906378217
27	0.28596294914832776
28	0.07459903021260723
29	0.3356956359567326
30	0.03729951510630362
31	0.03729951510630362
32	0.16163123212731567
33	0.02486634340420241
34	0.012433171702101205
35	0.0
36	0.08703220191470844
37	0.0
38	0.0
39	0.012433171702101205
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	8043.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.09797339301257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.40094618568894	77.70732313813254
2	5.396215257244235	9.07621534253388
3	0.8426966292134831	2.126072361059306
4	0.517445298639858	1.7406440382941688
5	0.3104671791839148	1.3054830287206265
6	0.05913660555884093	0.29839612085042894
7	0.10348905972797162	0.6092254134029591
8	0.05913660555884093	0.39786149446723856
9	0.07392075694855116	0.5594927265945543
>10	0.20697811945594324	3.319656844461022
>50	0.0	0.0
>100	0.029568302779420463	2.859629491483277
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGGGTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	127	1.5790128061668531	No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	103	1.2806166853164243	No Hit
AGGGTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG	36	0.4475941812756434	No Hit
AGGGTCGGGACTAATCCTAGCCCTAGCCCTACACAAATAT	32	0.39786149446723856	No Hit
AGGGTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT	31	0.3854283227651374	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	28	0.34812880765883375	No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA	23	0.28596294914832776	No Hit
AGGGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA	20	0.24866343404202412	No Hit
AGGGTCGGGGTTGTGTGGCTTTGGCCCTAAGCCATTGTCT	16	0.19893074723361928	No Hit
AGGGTCGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGT	15	0.18649757553151808	No Hit
AGGGTCGGGGTATGTGTGTCTGTTTGTCTGGTATGATGTG	12	0.14919806042521447	No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	12	0.14919806042521447	No Hit
AGGGTCGGGATGCCACAACTAGATACATCAACATGATTTA	11	0.13676488872311326	No Hit
AGGGTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11	0.13676488872311326	No Hit
AGGGTCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC	10	0.12433171702101206	No Hit
AGGGTCGGGATAGCAGGATTTGTTCACTGATTCCCATTAT	10	0.12433171702101206	No Hit
AGGGTCGGGGGGGCTGGGGAGATGGCTCAGTGGTTAAGAG	9	0.11189854531891084	No Hit
AGGGTCGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG	9	0.11189854531891084	No Hit
AGGGTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9	0.11189854531891084	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAG	9	0.11189854531891084	No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG	9	0.11189854531891084	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.012433171702101207	0.0	0.0	0.0	0.0
21	0.012433171702101207	0.0	0.0	0.0	0.0
22	0.012433171702101207	0.0	0.0	0.0	0.0
23	0.012433171702101207	0.0	0.0	0.0	0.0
24	0.012433171702101207	0.0	0.0	0.0	0.0
25	0.012433171702101207	0.0	0.0	0.0	0.0
26	0.012433171702101207	0.0	0.0	0.0	0.0
27	0.012433171702101207	0.0	0.0	0.0	0.0
28	0.012433171702101207	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGTCG	795	0.0	34.000004	1
ATCCTCT	25	1.8543858E-4	34.0	16
TCCTCTT	25	1.8543858E-4	34.0	17
GGGTCGG	785	0.0	34.0	2
GGGTCAC	35	9.229316E-7	34.0	7
GTCACAG	25	1.8543858E-4	34.0	9
ACAGATC	20	0.0026274757	34.0	12
CGGGGGT	50	3.2014214E-10	34.0	6
CGGGGGG	55	2.1827873E-11	34.0	6
CGGGGGC	25	1.8543858E-4	34.0	6
CGGGGGA	30	1.30865465E-5	34.0	6
CGGGGCT	20	0.0026274757	34.0	6
CGGGGCC	30	1.30865465E-5	34.0	6
CGGGGAT	20	0.0026274757	34.0	6
CGGGGAG	25	1.8543858E-4	34.0	6
ATTGGGC	20	0.0026274757	34.0	33
GGTCACA	30	1.30865465E-5	34.0	8
GGGAGTT	25	1.8543858E-4	34.0	7
GATTGGG	20	0.0026274757	34.0	32
CGGGCTC	20	0.0026274757	34.0	6
>>END_MODULE