SRR1525822_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1525822_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5094
Sequences flagged as poor quality	0
Sequence length	56
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGTTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	178	3.4943070278759327	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	160	3.1409501374165685	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	96	1.884570082449941	No Hit
GGGTTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	63	1.2367491166077738	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	55	1.0797016097369454	No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	38	0.745975657636435	No Hit
GGGTTTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG	33	0.6478209658421673	No Hit
GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30	0.5889281507656066	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	30	0.5889281507656066	No Hit
GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	19	0.3729878288182175	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	16	0.3140950137416569	No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTTTGGGAAGCAGT	15	0.2944640753828033	No Hit
GGGTTTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	15	0.2944640753828033	No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTCTTTTTTTTTTTTTTT	15	0.2944640753828033	No Hit
GGGTTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	14	0.27483313702394974	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAAAAA	14	0.27483313702394974	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	13	0.2552021986650962	No Hit
GGGTTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGCTGTAGCGCCGGAAGA	13	0.2552021986650962	No Hit
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	13	0.2552021986650962	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	11	0.21594032194738907	No Hit
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11	0.21594032194738907	No Hit
GGGTTTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTTATATG	10	0.19630938358853553	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAAAAA	10	0.19630938358853553	No Hit
GGGTTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	9	0.17667844522968199	No Hit
GGGTTTGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC	9	0.17667844522968199	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTA	9	0.17667844522968199	No Hit
GGGTTTGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTAGATCTTGGAG	8	0.15704750687082844	No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTCTTTTTTTTTTTTTTTT	8	0.15704750687082844	No Hit
GGGTTTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTCGCAGCATTTTATATG	7	0.13741656851197487	No Hit
GGGTTTGGGTCACAGATCCTCTTAAGGAGATTGGGCGGATTGAACAAAAACTCATC	7	0.13741656851197487	No Hit
GGGTTTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	7	0.13741656851197487	No Hit
GGGTTTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	7	0.13741656851197487	No Hit
GGGTTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCCTTGTAGCGCCGGAAGA	6	0.11778563015312131	No Hit
GGGTTTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTATGCCGTCTTCTGCTTGG	6	0.11778563015312131	Illumina Single End Adapter 1 (95% over 24bp)
GGGTTTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTCGCAGCATTTGATATG	6	0.11778563015312131	No Hit
GGGTTTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	6	0.11778563015312131	No Hit
GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA	6	0.11778563015312131	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTAGC	25	1.5826065E-5	51.081635	41
CCGTTGT	15	0.0072973417	51.08163	38
GATTGAC	15	0.0072973417	51.08163	41
CGTTGTA	15	0.0072973417	51.08163	39
GTTGTAG	15	0.0072973417	51.08163	40
CCGGAAG	25	1.6832702E-5	50.56566	49
GCCGGAA	25	1.6832702E-5	50.56566	48
CGCCGGA	25	1.6832702E-5	50.56566	47
GCGCCGG	30	7.869712E-7	50.565655	46
TGTAGCG	30	7.869712E-7	50.565655	42
ATTGACC	15	0.0076009594	50.565655	42
AGCGCCG	30	7.869712E-7	50.565655	45
TAGCGCC	30	8.4531894E-7	50.06	44
GGAGAAA	25	1.7891825E-5	50.06	20
TCGATTC	25	1.7891825E-5	50.06	20
TTCGTGG	25	1.7891825E-5	50.06	15
AGTGGTA	60	0.0	50.06	14
TTCGATT	25	1.7891825E-5	50.06	19
TCGTGGA	25	1.7891825E-5	50.06	16
TGGAGAA	25	1.7891825E-5	50.06	19

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content