##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525822_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5094
Sequences flagged as poor quality	0
Sequence length	56
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.27345897133883	38.0	38.0	40.0	33.0	40.0
2	37.43384373773066	38.0	38.0	40.0	34.0	40.0
3	37.215155084413034	38.0	38.0	40.0	33.0	40.0
4	36.110129564193166	38.0	35.0	39.0	31.0	40.0
5	37.563800549666276	39.0	38.0	40.0	35.0	40.0
6	37.09383588535532	38.0	37.0	40.0	33.0	40.0
7	37.063407930899096	38.0	38.0	40.0	33.0	40.0
8	36.784648606203376	38.0	36.0	40.0	32.0	40.0
9	36.67510797016097	38.0	36.0	40.0	31.0	40.0
10	36.15665488810365	38.0	36.0	40.0	31.0	40.0
11	34.8680800942285	38.0	35.0	40.0	25.0	40.0
12	33.523753435414214	38.0	32.0	39.0	19.0	40.0
13	32.89791912053396	37.0	31.0	39.0	17.0	40.0
14	33.505889281507656	38.0	32.0	39.0	18.0	40.0
15	32.06556733411857	37.0	29.0	39.0	15.0	40.0
16	31.883981154299175	36.0	29.0	39.0	15.0	40.0
17	31.27875932469572	36.0	27.0	39.0	15.0	40.0
18	31.010993325480957	35.0	26.0	39.0	14.0	40.0
19	31.464468001570474	36.0	27.0	39.0	15.0	40.0
20	32.57813113466824	37.0	30.0	39.0	18.0	40.0
21	33.108951707891634	38.0	32.0	39.0	17.0	40.0
22	32.245190420102084	38.0	30.0	39.0	15.0	40.0
23	32.967020023557126	38.0	32.0	39.0	17.0	40.0
24	32.17137809187279	37.0	30.0	39.0	15.0	40.0
25	32.61739301138594	38.0	31.0	39.0	16.0	40.0
26	32.4006674519042	37.0	31.0	39.0	16.0	40.0
27	32.77836670592854	38.0	32.0	39.0	16.0	40.0
28	32.83451118963487	38.0	32.0	39.0	16.0	40.0
29	31.985865724381625	38.0	30.0	39.0	11.0	40.0
30	31.933647428347076	37.0	30.0	39.0	11.0	40.0
31	32.14310954063604	38.0	31.0	39.0	9.0	40.0
32	32.04161758932077	38.0	31.0	39.0	4.0	40.0
33	31.672555948174324	36.0	30.0	39.0	4.0	40.0
34	31.424617196702002	36.0	30.0	39.0	4.0	40.0
35	31.744601491951315	37.0	31.0	39.0	4.0	40.0
36	30.311739301138594	36.0	29.0	38.0	4.0	40.0
37	29.851590106007066	35.0	27.0	38.0	4.0	40.0
38	29.175893207695328	35.0	25.0	38.0	4.0	40.0
39	27.868080094228503	34.0	19.0	38.0	4.0	40.0
40	25.84687868080094	32.0	10.0	38.0	4.0	40.0
41	22.66372202591284	27.0	4.0	36.0	4.0	39.0
42	23.594817432273263	28.0	4.0	35.0	4.0	39.0
43	24.482921083627797	30.0	4.0	36.0	4.0	39.0
44	23.996859049862582	30.0	4.0	37.0	4.0	39.0
45	24.39006674519042	30.0	4.0	37.0	4.0	39.0
46	22.59383588535532	27.0	4.0	35.0	4.0	39.0
47	22.08441303494307	26.0	4.0	35.0	4.0	39.0
48	22.458578720062818	26.0	4.0	35.0	4.0	39.0
49	21.573027090694936	26.0	4.0	35.0	4.0	39.0
50	22.191401648998824	27.0	4.0	35.0	4.0	39.0
51	22.543384373773065	28.0	4.0	36.0	4.0	39.0
52	22.31782489202984	28.0	4.0	35.0	4.0	39.0
53	22.38457008244994	29.0	4.0	36.0	4.0	39.0
54	21.957989791912052	28.0	4.0	36.0	4.0	39.0
55	21.897330192383198	28.0	4.0	36.0	4.0	39.0
56	20.775618374558302	24.0	4.0	36.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	0.5742658730158823
1	2	-0.2981779100529067
1	3	0.07619708994708674
1	4	-0.4165773809523756
1	5	0.5852447089947148
1	6	1.9771560846560803
1	7	2.3977215608465627
1	8	2.2801025132275043
1	9	3.0053538359788305
1	10	3.2201752645502637
1	11	3.44408399470899
1	12	-2.624580026455021
1	13	-14.891269841269848
1	14	-3.2444212962962986
1	15	4.214120370370377
1	16	-0.37736772486770676
1	17	-5.798062169312171
1	18	-3.116898148148149
1	19	1.1893716931216858
1	20	-0.2572685185185257
1	21	6.797093253968253
1	22	7.716216931216934
1	23	4.695876322751332
1	24	5.554828042328037
1	25	6.957731481481481
1	26	8.601111111111116
1	27	7.888948412698419
1	28	5.86843584656085
1	29	7.6753472222222285
1	30	7.216990740740734
1	31	8.442863756613757
1	32	4.878921957671967
1	33	7.619675925925922
1	34	7.9611904761904775
1	35	2.268472222222229
1	36	1.5095866402116336
1	37	-3.3455291005290917
1	38	-1.822572751322756
1	39	-4.228336640211644
1	40	-14.892989417989424
1	41	-7.469510582010587
1	42	-8.020988756613747
1	43	-13.246865079365087
1	44	-14.98660714285715
1	45	-12.40231812169312
1	46	-12.7601917989418
1	47	-15.469169973544979
1	48	-11.556980820105817
1	49	-15.382857142857148
1	50	-12.169176587301589
1	51	-11.884725529100523
1	52	-11.77930555555555
1	53	-11.90019841269841
1	54	-11.39751984126985
1	55	-10.980241402116404
1	56	-8.513141534391531
2	1	2.4492658730158823
2	2	1.7018220899470933
2	3	2.2845304232804224
2	4	2.4584226190476244
2	5	1.9602447089947148
2	6	2.935489417989416
2	7	2.9810548941798984
2	8	3.11343584656084
2	9	3.0886871693121662
2	10	4.095175264550264
2	11	6.110750661375654
2	12	2.083753306878311
2	13	3.400396825396818
2	14	7.630578703703701
2	15	7.297453703703713
2	16	4.705965608465625
2	17	8.285271164021161
2	18	6.299768518518519
2	19	5.689371693121686
2	20	7.117731481481474
2	21	5.755426587301589
2	22	8.841216931216934
2	23	8.154209656084667
2	24	7.596494708994701
2	25	8.124398148148146
2	26	6.809444444444452
2	27	6.722281746031754
2	28	7.701769179894185
2	29	7.842013888888893
2	30	6.175324074074069
2	31	7.651197089947093
2	32	6.795588624338631
2	33	8.203009259259257
2	34	7.0861904761904775
2	35	4.185138888888897
2	36	5.217919973544966
2	37	3.4878042328042405
2	38	6.719093915343912
2	39	4.604996693121688
2	40	0.8153439153439095
2	41	5.238822751322747
2	42	4.229011243386253
2	43	3.7531349206349134
2	44	-2.3199404761904816
2	45	3.5976818783068794
2	46	2.3231415343915316
2	47	-0.6358366402116467
2	48	2.8180191798941827
2	49	-1.96619047619048
2	50	2.997490079365079
2	51	3.823607804232811
2	52	3.679027777777783
2	53	3.5581349206349238
2	54	5.435813492063485
2	55	4.22809193121693
2	56	6.903525132275135
3	1	0.07426587301588228
3	2	-0.17317791005290673
3	3	-0.21546957671957756
3	4	-1.7915773809523756
3	5	0.2102447089947148
3	6	2.185489417989416
3	7	2.4810548941798984
3	8	2.61343584656084
3	9	2.5886871693121662
3	10	2.8451752645502637
3	11	4.735750661375654
3	12	6.958753306878311
3	13	4.650396825396818
3	14	7.005578703703701
3	15	1.422453703703713
3	16	6.955965608465625
3	17	7.410271164021161
3	18	7.299768518518519
3	19	4.314371693121686
3	20	6.867731481481474
3	21	4.005426587301589
3	22	2.341216931216934
3	23	4.654209656084667
3	24	2.721494708994701
3	25	5.1243981481481455
3	26	6.309444444444452
3	27	4.722281746031754
3	28	4.451769179894185
3	29	4.842013888888893
3	30	6.300324074074069
3	31	5.276197089947093
3	32	0.17058862433863098
3	33	5.828009259259257
3	34	3.4611904761904775
3	35	3.935138888888897
3	36	2.4679199735449657
3	37	1.9878042328042405
3	38	3.2190939153439118
3	39	2.604996693121688
3	40	7.3153439153439095
3	41	6.488822751322747
3	42	4.979011243386253
3	43	3.3781349206349134
3	44	6.930059523809518
3	45	7.847681878306879
3	46	5.823141534391532
3	47	6.114163359788353
3	48	6.068019179894183
3	49	7.78380952380952
3	50	6.747490079365079
3	51	7.823607804232811
3	52	6.304027777777783
3	53	8.058134920634924
3	54	6.685813492063485
3	55	6.22809193121693
3	56	9.278525132275135
4	1	-0.05073412698411772
4	2	-0.2981779100529067
4	3	-0.46546957671957756
4	4	0.20842261904762438
4	5	-0.9147552910052852
4	6	1.935489417989416
4	7	2.3560548941798984
4	8	1.73843584656084
4	9	1.7136871693121662
4	10	-0.2798247354497363
4	11	4.485750661375654
4	12	5.083753306878311
4	13	3.150396825396818
4	14	5.380578703703701
4	15	4.297453703703713
4	16	4.205965608465625
4	17	4.910271164021161
4	18	6.924768518518519
4	19	4.064371693121686
4	20	5.742731481481474
4	21	4.755426587301589
4	22	6.466216931216934
4	23	6.279209656084667
4	24	5.846494708994701
4	25	3.4993981481481455
4	26	4.434444444444452
4	27	5.722281746031754
4	28	1.7017691798941854
4	29	1.9670138888888928
4	30	3.4253240740740694
4	31	-0.47380291005290687
4	32	2.170588624338631
4	33	2.8280092592592574
4	34	2.0861904761904775
4	35	3.810138888888897
4	36	4.342919973544966
4	37	0.7378042328042405
4	38	0.09409391534391176
4	39	-6.520003306878312
4	40	-5.8096560846560905
4	41	2.488822751322747
4	42	-0.8959887566137468
4	43	0.5031349206349134
4	44	1.9300595238095184
4	45	0.9726818783068794
4	46	2.6981415343915316
4	47	3.1141633597883533
4	48	4.693019179894183
4	49	3.03380952380952
4	50	2.872490079365079
4	51	-4.426392195767189
4	52	-3.320972222222217
4	53	-2.6918650793650762
4	54	-2.8141865079365154
4	55	-1.5219080687830697
4	56	-0.471474867724865
5	1	2.4492658730158823
5	2	2.0768220899470933
5	3	2.1595304232804224
5	4	2.4584226190476244
5	5	1.9602447089947148
5	6	2.810489417989416
5	7	3.3560548941798984
5	8	3.86343584656084
5	9	3.4636871693121662
5	10	4.220175264550264
5	11	6.860750661375654
5	12	7.708753306878311
5	13	7.400396825396818
5	14	7.380578703703701
5	15	9.297453703703713
5	16	9.705965608465625
5	17	10.660271164021161
5	18	9.299768518518519
5	19	8.939371693121686
5	20	9.242731481481474
5	21	8.380426587301589
5	22	8.341216931216934
5	23	7.154209656084667
5	24	9.221494708994701
5	25	6.6243981481481455
5	26	8.184444444444452
5	27	8.222281746031754
5	28	6.576769179894185
5	29	8.592013888888893
5	30	7.300324074074069
5	31	5.901197089947093
5	32	7.045588624338631
5	33	6.078009259259257
5	34	5.8361904761904775
5	35	6.310138888888897
5	36	7.467919973544966
5	37	8.73780423280424
5	38	9.094093915343912
5	39	12.104996693121688
5	40	12.31534391534391
5	41	14.863822751322747
5	42	16.229011243386253
5	43	15.128134920634913
5	44	11.555059523809518
5	45	14.47268187830688
5	46	16.19814153439153
5	47	13.239163359788353
5	48	14.068019179894183
5	49	16.15880952380952
5	50	15.747490079365079
5	51	16.44860780423281
5	52	14.804027777777783
5	53	12.058134920634924
5	54	16.310813492063485
5	55	12.47809193121693
5	56	12.278525132275135
6	1	2.407599206349218
6	2	1.8129332010582075
6	3	1.687308201058201
6	4	2.41675595238096
6	5	1.696355820105829
6	6	3.0327116402116374
6	7	3.45327711640212
6	8	3.8356580687830615
6	9	3.9497982804232805
6	10	2.9979530423280423
6	11	6.721861772486768
6	12	8.430975529100532
6	13	7.9420634920634825
6	14	8.144467592592587
6	15	7.936342592592599
6	16	6.5115211640211825
6	17	7.757493386243382
6	18	8.827546296296298
6	19	4.3004828042328
6	20	6.13162037037036
6	21	4.630426587301589
6	22	6.827328042328048
6	23	5.584765211640224
6	24	7.165939153439144
6	25	2.6799537037037027
6	26	6.434444444444452
6	27	6.388948412698419
6	28	5.923991402116407
6	29	4.6753472222222285
6	30	6.661435185185184
6	31	6.720641534391536
6	32	4.601144179894188
6	33	6.841898148148143
6	34	4.4611904761904775
6	35	3.2684722222222327
6	36	5.398475529100523
6	37	5.265582010582019
6	38	5.788538359788355
6	39	7.716107804232802
6	40	3.3847883597883524
6	41	11.586044973544968
6	42	11.03456679894181
6	43	10.753134920634913
6	44	11.513392857142854
6	45	10.8199041005291
6	46	11.35091931216931
6	47	13.308607804232796
6	48	11.831908068783072
6	49	13.950476190476184
6	50	12.997490079365079
6	51	10.893052248677254
6	52	12.220694444444447
6	53	10.099801587301592
6	54	9.269146825396817
6	55	10.797536375661373
6	56	8.875747354497356
7	1	0.9492658730158823
7	2	0.20182208994709327
7	3	-0.46546957671957756
7	4	-2.4165773809523756
7	5	0.4602447089947148
7	6	1.060489417989416
7	7	1.7310548941798984
7	8	2.11343584656084
7	9	3.0886871693121662
7	10	2.5951752645502637
7	11	5.110750661375654
7	12	6.833753306878311
7	13	6.525396825396818
7	14	7.630578703703701
7	15	7.547453703703713
7	16	8.330965608465625
7	17	6.910271164021161
7	18	8.924768518518519
7	19	7.189371693121686
7	20	4.367731481481474
7	21	5.005426587301589
7	22	3.341216931216934
7	23	2.7792096560846673
7	24	6.471494708994701
7	25	5.1243981481481455
7	26	6.559444444444452
7	27	5.097281746031754
7	28	3.5767691798941854
7	29	2.467013888888893
7	30	3.9253240740740694
7	31	1.5261970899470931
7	32	-1.829411375661369
7	33	-2.1719907407407426
7	34	-2.2888095238095225
7	35	-1.439861111111103
7	36	0.9679199735449657
7	37	-0.012195767195759544
7	38	2.3440939153439118
7	39	2.229996693121688
7	40	-2.8096560846560905
7	41	5.488822751322747
7	42	2.9790112433862532
7	43	2.7531349206349134
7	44	4.055059523809518
7	45	2.7226818783068794
7	46	1.4481415343915316
7	47	2.6141633597883533
7	48	3.5680191798941827
7	49	3.90880952380952
7	50	4.372490079365079
7	51	4.448607804232811
7	52	3.554027777777783
7	53	4.683134920634924
7	54	3.5608134920634846
7	55	5.10309193121693
7	56	3.903525132275135
8	1	1.1992658730158823
8	2	0.8268220899470933
8	3	-0.34046957671957756
8	4	-1.0415773809523756
8	5	0.08524470899471481
8	6	1.435489417989416
8	7	2.6060548941798984
8	8	2.23843584656084
8	9	2.7136871693121662
8	10	2.2201752645502637
8	11	4.235750661375654
8	12	5.208753306878311
8	13	4.525396825396818
8	14	4.755578703703701
8	15	6.797453703703713
8	16	6.830965608465625
8	17	6.160271164021161
8	18	8.674768518518519
8	19	6.814371693121686
8	20	5.242731481481474
8	21	7.005426587301589
8	22	7.966216931216934
8	23	8.529209656084667
8	24	3.846494708994701
8	25	7.8743981481481455
8	26	6.934444444444452
8	27	6.722281746031754
8	28	6.076769179894185
8	29	6.467013888888893
8	30	8.67532407407407
8	31	5.276197089947093
8	32	3.920588624338631
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116	9	2.0053538359788305
116	10	1.386841931216928
116	11	4.610750661375654
116	12	1.208753306878311
116	13	3.108730158730154
116	14	3.9222453703703657
116	15	-4.452546296296287
116	16	3.6226322751322897
116	17	4.868604497354497
116	18	3.716435185185187
116	19	3.85603835978835
116	20	6.242731481481474
116	21	4.797093253968253
116	22	1.716216931216934
116	23	4.695876322751332
116	24	3.0548280423280367
116	25	-4.3756018518518545
116	26	-2.065555555555548
116	27	-0.4443849206349135
116	28	0.2017691798941854
116	29	-2.157986111111107
116	30	1.3836574074074015
116	31	-2.723802910052907
116	32	-2.287744708994701
116	33	-1.8803240740740748
116	34	-0.03880952380952252
116	35	-1.064861111111103
116	36	2.676253306878298
116	37	2.9878042328042405
116	38	-0.3225727513227561
116	39	2.43833002645502
116	40	6.773677248677242
116	41	5.197156084656079
116	42	5.312344576719585
116	43	-5.080198412698419
116	44	-3.3199404761904816
116	45	-1.4023181216931206
116	46	-3.0935251322751363
116	47	-8.802503306878313
116	48	-2.3903141534391494
116	49	-1.882857142857148
116	50	1.497490079365079
116	51	0.6152744708994788
116	52	0.38736111111111526
116	53	0.9331349206349238
116	54	0.43581349206348463
116	55	-0.48024140211640187
116	56	4.320191798941803
117	1	-1.7257341269841149
117	2	-5.898177910052908
117	3	-6.090469576719578
117	4	-5.916577380952376
117	5	-6.214755291005286
117	6	-0.38951058201058686
117	7	0.23105489417989844
117	8	-0.1865641534391571
117	9	-5.561312830687832
117	10	-8.779824735449736
117	11	-9.589249338624345
117	12	-7.59124669312169
117	13	-12.12460317460318
117	14	-8.5444212962963
117	15	-11.352546296296286
117	16	-14.644034391534374
117	17	-6.464728835978839
117	18	-14.950231481481481
117	19	-9.410628306878316
117	20	-8.957268518518525
117	21	-7.569573412698411
117	22	-7.483783068783065
117	23	-7.970790343915333
117	24	-6.478505291005298
117	25	-12.875601851851854
117	26	-12.565555555555548
117	27	-14.277718253968246
117	28	-14.298230820105815
117	29	-12.657986111111107
117	30	-12.44967592592593
117	31	-12.723802910052907
117	32	-13.55441137566137
117	33	-12.046990740740743
117	34	-12.538809523809523
117	35	-19.064861111111103
117	36	-17.357080026455034
117	37	-16.71219576719576
117	38	-15.855906084656088
117	39	-13.595003306878311
117	40	-12.25965608465609
117	41	-9.436177248677254
117	42	-10.320988756613747
117	43	-11.246865079365087
117	44	-10.019940476190481
117	45	-12.40231812169312
117	46	-10.026858465608468
117	47	-9.735836640211646
117	48	-10.256980820105817
117	49	-11.91619047619048
117	50	-11.20250992063492
117	51	-10.551392195767189
117	52	-10.845972222222217
117	53	-10.966865079365077
117	54	-9.664186507936515
117	55	-9.24690806878307
117	56	-9.746474867724865
118	1	1.5742658730158823
118	2	0.20182208994709327
118	3	1.4095304232804224
118	4	-0.16657738095237562
118	5	0.8352447089947148
118	6	1.560489417989416
118	7	1.9810548941798984
118	8	1.61343584656084
118	9	2.0886871693121662
118	10	2.7201752645502637
118	11	2.860750661375654
118	12	-0.541246693121689
118	13	5.025396825396818
118	14	1.2555787037037014
118	15	-0.952546296296287
118	16	4.455965608465625
118	17	6.785271164021161
118	18	2.049768518518519
118	19	6.189371693121686
118	20	4.742731481481474
118	21	8.130426587301589
118	22	9.216216931216934
118	23	8.529209656084667
118	24	9.221494708994701
118	25	7.8743981481481455
118	26	8.434444444444452
118	27	8.472281746031754
118	28	6.451769179894185
118	29	9.092013888888893
118	30	9.30032407407407
118	31	9.026197089947093
118	32	8.545588624338631
118	33	8.953009259259257
118	34	9.211190476190477
118	35	8.685138888888897
118	36	10.592919973544966
118	37	11.23780423280424
118	38	10.094093915343912
118	39	14.354996693121688
118	40	14.44034391534391
118	41	15.863822751322747
118	42	15.979011243386253
118	43	5.503134920634913
118	44	8.930059523809518
118	45	13.09768187830688
118	46	12.323141534391532
118	47	2.6141633597883533
118	48	8.943019179894183
118	49	8.28380952380952
118	50	12.997490079365079
118	51	15.198607804232811
118	52	15.304027777777783
118	53	12.683134920634924
118	54	14.935813492063485
118	55	16.85309193121693
118	56	11.903525132275135
119	1	0.407599206349218
119	2	0.03515542328042898
119	3	0.24286375661375814
119	4	-5.5832440476190435
119	5	-0.7480886243386209
119	6	1.1438227513227517
119	7	1.5643882275132341
119	8	1.9467691798941757
119	9	2.172020502645502
119	10	-0.7798247354497363
119	11	0.9440839947089898
119	12	0.8754199735449788
119	13	2.9420634920634825
119	14	-3.4110879629629665
119	15	-9.285879629629619
119	16	-5.7107010582010425
119	17	-2.798062169312171
119	18	-8.950231481481481
119	19	-8.143961640211646
119	20	-5.423935185185194
119	21	1.2970932539682565
119	22	2.382883597883602
119	23	1.3625429894179995
119	24	1.0548280423280332
119	25	-0.2089351851851866
119	26	7.767777777777788
119	27	2.3889484126984186
119	28	7.035102513227521
119	29	6.342013888888893
119	30	7.883657407407405
119	31	-4.057136243386239
119	32	-2.954411375661369
119	33	-2.3803240740740748
119	34	-4.20547619047619
119	35	-5.731527777777771
119	36	-1.1570800264550343
119	37	-0.5121957671957595
119	38	-1.9892394179894204
119	39	1.93833002645502
119	40	3.9403439153439095
119	41	-8.969510582010587
119	42	-8.85432208994708
119	43	-16.58019841269842
119	44	-16.153273809523814
119	45	-9.40231812169312
119	46	-9.093525132275134
119	47	-8.135836640211647
119	48	-11.723647486772483
119	49	-7.71619047619048
119	50	-7.002509920634921
119	51	-6.884725529100523
119	52	-6.445972222222217
119	53	-6.90019841269841
119	54	-8.064186507936515
119	55	-6.313574735449736
119	56	-14.346474867724865
120	1	0.5028373015873129
120	2	0.13039351851852388
120	3	0.33810185185185304
120	4	0.08342261904762438
120	5	0.013816137566145414
120	6	1.2390608465608466
120	7	1.659626322751329
120	8	2.0420072751322706
120	9	2.267258597883597
120	10	2.6487466931216943
120	11	3.1821792328042235
120	12	-4.934103835978831
120	13	3.8468253968253876
120	14	1.8270072751322708
120	15	-0.0954034391534293
120	16	-4.186891534391517
120	17	1.963842592592588
120	18	-0.378802910052908
120	19	1.6179431216931128
120	20	0.3855886243386166
120	21	4.344712301587304
120	22	5.573359788359788
120	23	6.029209656084667
120	24	5.007208994708986
120	25	4.1243981481481455
120	26	7.005873015873021
120	27	4.579424603174608
120	28	5.701769179894185
120	29	6.770585317460323
120	30	6.264609788359785
120	31	7.704768518518524
120	32	3.7598743386243463
120	33	6.8101521164021115
120	34	7.4611904761904775
120	35	6.935138888888897
120	36	9.271491402116396
120	37	8.773518518518525
120	38	10.772665343915342
120	39	11.890710978835973
120	40	13.226058201058194
120	41	-2.06474867724868
120	42	0.3361541005291109
120	43	-2.5325793650793713
120	44	-10.819940476190482
120	45	-0.40231812169312064
120	46	-2.712572751322753
120	47	-3.9929794973545043
120	48	-2.914123677248675
120	49	-12.71619047619048
120	50	-1.1453670634920634
120	51	3.448607804232811
120	52	4.125456349206356
120	53	1.2902777777777814
120	54	2.9358134920634846
120	55	4.067377645502646
120	56	8.93923941798942
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	11.0
5	4.0
6	28.0
7	15.0
8	42.0
9	92.0
10	46.0
11	26.0
12	29.0
13	27.0
14	39.0
15	44.0
16	40.0
17	41.0
18	55.0
19	75.0
20	70.0
21	80.0
22	119.0
23	146.0
24	147.0
25	163.0
26	188.0
27	208.0
28	169.0
29	172.0
30	196.0
31	221.0
32	267.0
33	252.0
34	322.0
35	324.0
36	322.0
37	334.0
38	364.0
39	416.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	98.93992932862191	0.3729878288182175	0.4907734589713388	0.19630938358853553
8	98.76325088339223	0.6085590891244602	0.4122497055359246	0.21594032194738907
9	95.95602669807617	1.1582253631723596	2.159403219473891	0.7263447192775815
10	28.4452296819788	26.40361209265803	38.59442481350609	6.556733411857087
11	21.829603455045152	34.04004711425206	5.712603062426385	38.417746368276404
12	36.82764036120927	46.58421672555948	5.35924617196702	11.228896741264233
13	9.403219473890852	26.109148017275224	7.695327836670593	56.79230467216333
14	10.522182960345503	69.3168433451119	8.775029446407538	11.385944248135061
15	53.10589627292447	17.412739104713072	17.787418655097614	11.693945967264838
16	9.628610729023384	47.57319709176656	32.796227156612304	10.00196502259776
17	24.828128069141624	16.872912983696718	41.17069338047535	17.12826556668631
18	26.658814291323125	15.292500981546919	11.798193953670985	46.25049077345897
19	11.64571877454831	16.33935585231736	30.63629222309505	41.37863315003928
20	15.998427672955975	28.282232704402517	45.263364779874216	10.455974842767295
21	15.940321947389085	18.276403612092658	23.87122104436592	41.91205339615234
22	17.648213584609344	21.73144876325088	40.73419709462112	19.88614055751865
23	17.981939536709852	33.49038084020416	40.16489988221437	8.362779740871613
24	10.247349823321555	38.22143698468787	15.076560659599528	36.454652532391044
25	12.131919905771497	25.65763643502159	44.012563800549664	18.19787985865724
26	52.729772191673206	26.09976433621367	12.39198743126473	8.77847604084839
27	19.520816967792616	50.09819324430479	12.077769049489396	18.3032207384132
28	32.724774244208874	50.804868472713	9.305064782096583	7.165292500981548
29	35.770440251572325	43.57311320754717	15.919811320754718	4.736635220125787
30	24.85753586166241	37.17822754961682	30.497150717233247	7.467085871487522
31	36.049479677989396	36.42254074219517	20.518358531317496	7.009621048497938
32	31.572746907520127	39.23031612016493	22.305124680934615	6.891812291380325
33	42.42185964222528	34.0082563396894	12.1092982111264	11.460585806958914
34	15.762578616352203	21.187106918238992	43.69103773584906	19.35927672955975
35	12.556494399685597	29.455688740420516	47.47494596187856	10.512870898015327
36	28.804241115256236	25.937561358727667	29.687806793638327	15.570390732377772
37	28.47590953785644	22.22222222222222	37.20747295968535	12.094395280235988
38	29.610696028313015	21.234762092017302	35.528902870625245	13.625639009044436
39	26.63652447414979	23.845095341065463	19.69726754472184	29.821112640062907
40	22.985547416353196	18.96654127895466	36.64620867155019	21.40170263314195
41	22.791344054000398	17.212626563430614	31.82449870954934	28.171530673019657
42	20.098425196850396	34.19291338582677	21.27952755905512	24.429133858267715
43	16.469893742621014	35.91105863833137	26.859504132231404	20.759543486816217
44	11.133004926108374	41.2807881773399	34.77832512315271	12.807881773399016
45	24.95084545812033	26.30751081399921	37.200157294534016	11.541486433346442
46	13.177073074650384	36.57671853456766	21.37088831987394	28.875320070908018
47	14.003944773175542	34.418145956607496	17.08086785009862	34.49704142011834
48	29.756289308176097	27.122641509433965	20.91194968553459	22.209119496855344
49	12.229224103977945	35.21071287908625	21.425758172508864	31.134304844426943
50	10.422812192723697	38.95771878072763	23.107177974434613	27.512291052114062
51	15.307328605200945	41.469661150512216	18.93223010244287	24.29078014184397
52	15.59488692232055	55.024582104228124	19.724680432645034	9.655850540806293
53	10.055096418732782	53.46320346320347	23.415977961432507	13.06572215663125
54	10.422812192723697	39.64601769911504	30.363815142576208	19.567354965585054
55	16.15187881172536	29.86425339366516	34.11371237458194	19.870155420027544
56	15.036410155481207	32.82818342845896	32.65105294233418	19.484353473725644
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.5
9	2.0
10	25.5
11	49.0
12	57.0
13	62.5
14	60.0
15	52.5
16	45.0
17	42.5
18	40.0
19	42.0
20	44.0
21	45.0
22	43.5
23	41.0
24	48.0
25	55.0
26	52.0
27	49.0
28	54.0
29	62.0
30	65.0
31	63.5
32	62.0
33	70.5
34	79.0
35	104.0
36	129.0
37	123.0
38	110.0
39	103.0
40	107.0
41	111.0
42	132.5
43	154.0
44	255.5
45	357.0
46	498.0
47	693.5
48	748.0
49	599.5
50	451.0
51	391.5
52	332.0
53	347.5
54	328.0
55	293.0
56	227.0
57	161.0
58	152.5
59	144.0
60	111.0
61	78.0
62	62.0
63	43.5
64	41.0
65	37.0
66	33.0
67	22.0
68	11.0
69	11.5
70	12.0
71	8.0
72	4.0
73	4.0
74	2.5
75	1.0
76	0.5
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.4515115822536317
16	0.09815469179426776
17	0.058892815076560655
18	0.0
19	0.03926187671770711
20	0.11778563015312131
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.03926187671770711
27	0.03926187671770711
28	0.0
29	0.11778563015312131
30	0.09815469179426776
31	0.019630938358853555
32	0.019630938358853555
33	0.13741656851197487
34	0.11778563015312131
35	0.09815469179426776
36	0.019630938358853555
37	0.17667844522968199
38	0.15704750687082844
39	0.13741656851197487
40	0.8441303494307029
41	1.1189634864546525
42	0.27483313702394974
43	0.23557126030624262
44	0.3729878288182175
45	0.15704750687082844
46	0.3337259521005104
47	0.47114252061248524
48	0.11778563015312131
49	0.3140950137416569
50	0.17667844522968199
51	0.35335689045936397
52	0.17667844522968199
53	0.23557126030624262
54	0.17667844522968199
55	0.21594032194738907
56	0.2552021986650962
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	5094.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.83195916764821
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.62456793406008	67.64821358460934
2	5.184791278915182	7.656065959952886
3	1.5155543738367456	3.356890459363958
4	0.42541877160329705	1.2563800549666275
5	0.2658867322520606	0.9815469179426776
6	0.1329433661260303	0.5889281507656066
7	0.10635469290082426	0.5496662740478995
8	0.05317734645041213	0.3140950137416569
9	0.07976601967561818	0.5300353356890459
>10	0.47859611805370916	6.281900274833137
>50	0.07976601967561818	4.20102080879466
>100	0.05317734645041213	6.635257165292501
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGTTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	178	3.4943070278759327	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	160	3.1409501374165685	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	96	1.884570082449941	No Hit
GGGTTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	63	1.2367491166077738	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	55	1.0797016097369454	No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	38	0.745975657636435	No Hit
GGGTTTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG	33	0.6478209658421673	No Hit
GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30	0.5889281507656066	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	30	0.5889281507656066	No Hit
GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	19	0.3729878288182175	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	16	0.3140950137416569	No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTTTGGGAAGCAGT	15	0.2944640753828033	No Hit
GGGTTTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	15	0.2944640753828033	No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTCTTTTTTTTTTTTTTT	15	0.2944640753828033	No Hit
GGGTTTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	14	0.27483313702394974	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAAAAA	14	0.27483313702394974	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	13	0.2552021986650962	No Hit
GGGTTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGCTGTAGCGCCGGAAGA	13	0.2552021986650962	No Hit
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	13	0.2552021986650962	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	11	0.21594032194738907	No Hit
GGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11	0.21594032194738907	No Hit
GGGTTTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTTATATG	10	0.19630938358853553	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAAAAA	10	0.19630938358853553	No Hit
GGGTTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT	9	0.17667844522968199	No Hit
GGGTTTGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC	9	0.17667844522968199	No Hit
GGGTTTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTA	9	0.17667844522968199	No Hit
GGGTTTGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTAGATCTTGGAG	8	0.15704750687082844	No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTCTTTTTTTTTTTTTTTT	8	0.15704750687082844	No Hit
GGGTTTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTCGCAGCATTTTATATG	7	0.13741656851197487	No Hit
GGGTTTGGGTCACAGATCCTCTTAAGGAGATTGGGCGGATTGAACAAAAACTCATC	7	0.13741656851197487	No Hit
GGGTTTGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA	7	0.13741656851197487	No Hit
GGGTTTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	7	0.13741656851197487	No Hit
GGGTTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCCTTGTAGCGCCGGAAGA	6	0.11778563015312131	No Hit
GGGTTTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTATGCCGTCTTCTGCTTGG	6	0.11778563015312131	Illumina Single End Adapter 1 (95% over 24bp)
GGGTTTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTCGCAGCATTTGATATG	6	0.11778563015312131	No Hit
GGGTTTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	6	0.11778563015312131	No Hit
GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA	6	0.11778563015312131	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0785237534354142	0.0	0.0	0.0	0.0
11	0.21594032194738907	0.0	0.0	0.0	0.0
12	0.2552021986650962	0.0	0.0	0.0	0.0
13	0.2552021986650962	0.0	0.0	0.0	0.0
14	0.5104043973301924	0.0	0.0	0.0	0.0
15	0.5104043973301924	0.0	0.0	0.0	0.0
16	0.5300353356890459	0.0	0.0	0.0	0.0
17	0.5496662740478995	0.0	0.0	0.0	0.0
18	0.569297212406753	0.0	0.0	0.0	0.0
19	0.5889281507656066	0.0	0.0	0.0	0.0
20	0.6085590891244601	0.0	0.0	0.0	0.0
21	0.6281900274833137	0.0	0.0	0.0	0.0
22	0.6478209658421673	0.0	0.0	0.0	0.0
23	0.6674519042010209	0.0	0.0	0.0	0.0
24	0.7067137809187279	0.0	0.0	0.0	0.0
25	0.7067137809187279	0.0	0.0	0.0	0.0
26	0.745975657636435	0.0	0.0	0.0	0.0
27	0.8048684727129957	0.0	0.0	0.0	0.0
28	0.9030231645072635	0.0	0.0	0.0	0.0
29	0.9030231645072635	0.0	0.0	0.0	0.0
30	0.9422850412249706	0.0	0.0	0.0	0.0
31	1.0011778563015312	0.0	0.0	0.0	0.0
32	1.0208087946603848	0.0	0.0	0.0	0.0
33	1.0404397330192383	0.0	0.0	0.0	0.0
34	1.0404397330192383	0.0	0.0	0.0	0.0
35	1.0797016097369454	0.0	0.0	0.0	0.0
36	1.0797016097369454	0.0	0.0	0.0	0.0
37	1.0797016097369454	0.0	0.0	0.0	0.0
38	1.0797016097369454	0.0	0.0	0.0	0.0
39	1.0797016097369454	0.0	0.0	0.0	0.0
40	1.0797016097369454	0.0	0.0	0.0	0.0
41	1.099332548095799	0.0	0.0	0.0	0.0
42	1.138594424813506	0.0	0.0	0.0	0.0
43	1.1778563015312131	0.0	0.0	0.0	0.0
44	1.1974872398900667	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTAGC	25	1.5826065E-5	51.081635	41
CCGTTGT	15	0.0072973417	51.08163	38
GATTGAC	15	0.0072973417	51.08163	41
CGTTGTA	15	0.0072973417	51.08163	39
GTTGTAG	15	0.0072973417	51.08163	40
CCGGAAG	25	1.6832702E-5	50.56566	49
GCCGGAA	25	1.6832702E-5	50.56566	48
CGCCGGA	25	1.6832702E-5	50.56566	47
GCGCCGG	30	7.869712E-7	50.565655	46
TGTAGCG	30	7.869712E-7	50.565655	42
ATTGACC	15	0.0076009594	50.565655	42
AGCGCCG	30	7.869712E-7	50.565655	45
TAGCGCC	30	8.4531894E-7	50.06	44
GGAGAAA	25	1.7891825E-5	50.06	20
TCGATTC	25	1.7891825E-5	50.06	20
TTCGTGG	25	1.7891825E-5	50.06	15
AGTGGTA	60	0.0	50.06	14
TTCGATT	25	1.7891825E-5	50.06	19
TCGTGGA	25	1.7891825E-5	50.06	16
TGGAGAA	25	1.7891825E-5	50.06	19
>>END_MODULE