FastQCFastQC Report
Fri 17 Jun 2016
SRR1525821_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525821_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4730
Sequences flagged as poor quality0
Sequence length40
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGGAGTGCAGTGCTGGGTTTGGGACACGCCGGG110.23255813953488372No Hit
GGGTTTGGGGAGTGCAGTGCTGGGTTTGGGACACGCAGGG90.19027484143763213No Hit
GGGTTTGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.16913319238900634No Hit
GGGTTTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT70.14799154334038056No Hit
GGGTTTGGGCTCGTCTTAATCAGCAACATCTTTTGATACA70.14799154334038056No Hit
GGGTTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT60.12684989429175475No Hit
GGGTTTGGGAGTGCAGTGCTGGGTTTGGGACACGCAGGGA60.12684989429175475No Hit
GGGTTTGGGTAAATAGACGTCTCCAACTTCTGGAAGAGGA50.10570824524312897No Hit
GGGTTTGGGACTGTACATCTGGATTCTAGTTTGATCTTTC50.10570824524312897No Hit
GGGTTTGGGGAAAAAAAACAAAAAAAAAAAAAAAAAAAAA50.10570824524312897No Hit
GGGTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG50.10570824524312897No Hit
GGGTTTGGGATCACAACATTTCTGTGCTTTCTAGGATGTC50.10570824524312897No Hit
GGGTTTGGGATCAACATTAAGCTCAACTCAGCTTCTATCT50.10570824524312897No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGCA251.7511295E-433.957455
TGGGGAG251.7511295E-433.957456
TTTGGGT251.7511295E-433.957454
GGGGAGT251.7511295E-433.957457
GAAGCAT200.002532651633.9574479
GTATCAA200.002532651633.95744718
TTGGGGT358.250008E-733.9574475
TTGGGGG453.792593E-933.9574475
TGGGGGG200.002532651633.9574476
TTGGGAT405.6130375E-833.9574475
TTGGGAA800.033.9574475
GGAAGCA358.250008E-733.9574478
GTGGTAT200.002532651633.95744715
GGGAAGC453.792593E-933.9574477
GGGAACA200.002532651633.9574477
TGGGAGT200.002532651633.9574476
TGGGAAC200.002532651633.9574476
TGGGAAG601.8189894E-1233.9574436
TTTGGGA2350.032.512454
GGGTTTG4900.032.2249261
Produced by FastQC (version 0.11.5)