##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525821_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4730 Sequences flagged as poor quality 0 Sequence length 40 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.98076109936575 36.0 33.0 38.0 28.0 38.0 2 32.27251585623679 35.0 31.0 38.0 22.0 38.0 3 28.851162790697675 33.0 25.0 36.0 11.0 38.0 4 30.971035940803382 35.0 29.0 38.0 17.0 38.0 5 31.169133192389005 35.0 30.0 38.0 12.0 38.0 6 29.070824524312897 33.0 26.0 37.0 4.0 38.0 7 27.97463002114165 33.0 23.0 36.0 4.0 38.0 8 28.540380549682876 33.0 25.0 37.0 4.0 38.0 9 27.451797040169133 33.0 22.0 36.0 4.0 38.0 10 25.208033826638477 30.0 17.0 35.0 4.0 38.0 11 24.598097251585624 29.0 15.0 35.0 4.0 38.0 12 24.062579281183933 28.0 14.0 35.0 4.0 38.0 13 24.505285412262158 29.0 16.0 35.0 4.0 38.0 14 24.9661733615222 30.0 16.0 35.0 4.0 38.0 15 23.290063424947146 28.0 11.0 35.0 4.0 38.0 16 21.216701902748415 25.0 4.0 33.0 4.0 36.0 17 21.696405919661732 26.0 4.0 33.0 4.0 36.0 18 21.323890063424948 25.0 4.0 33.0 4.0 36.0 19 18.684989429175477 19.0 4.0 30.0 4.0 35.0 20 20.513319238900635 24.0 4.0 33.0 4.0 36.0 21 17.862367864693447 17.0 4.0 30.0 4.0 35.0 22 17.613742071881607 17.0 4.0 30.0 4.0 35.0 23 17.589429175475686 17.0 4.0 30.0 4.0 35.0 24 17.218393234672305 17.0 4.0 30.0 4.0 35.0 25 16.55031712473573 14.0 4.0 30.0 4.0 34.0 26 14.609725158562368 4.0 4.0 28.0 4.0 33.0 27 14.205496828752644 4.0 4.0 27.0 4.0 33.0 28 13.896828752642707 4.0 4.0 27.0 4.0 33.0 29 13.230021141649049 4.0 4.0 26.0 4.0 33.0 30 12.34630021141649 4.0 4.0 23.0 4.0 32.0 31 11.554334038054968 4.0 4.0 21.0 4.0 32.0 32 10.97399577167019 4.0 4.0 18.0 4.0 31.0 33 10.62875264270613 4.0 4.0 17.0 4.0 31.0 34 10.35200845665962 4.0 4.0 16.0 4.0 31.0 35 9.765116279069767 4.0 4.0 13.0 4.0 30.0 36 9.863002114164905 4.0 4.0 9.0 4.0 31.0 37 9.867653276955602 4.0 4.0 4.0 4.0 31.0 38 9.58245243128964 4.0 4.0 4.0 4.0 31.0 39 9.236786469344608 4.0 4.0 4.0 4.0 31.0 40 9.209090909090909 4.0 4.0 4.0 4.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.7652976190476224 1 2 0.2822420634920597 1 3 -7.520188492063497 1 4 1.5188293650793625 1 5 -1.0529861111111138 1 6 0.530198412698418 1 7 5.321299603174598 1 8 -0.3580257936507927 1 9 3.2515873015873034 1 10 2.7998809523809562 1 11 0.732361111111107 1 12 -4.743958333333339 1 13 -9.882351190476188 1 14 -7.084950396825402 1 15 -6.162410714285709 1 16 -5.919742063492063 1 17 -10.022400793650794 1 18 -10.812103174603164 1 19 -10.562807539682534 1 20 -4.286408730158726 1 21 -1.5366468253968328 1 22 -1.8200992063492034 1 23 -2.073184523809525 1 24 -7.029821428571429 1 25 -5.661140873015869 1 26 -2.41811507936508 1 27 0.5937003968253993 1 28 2.3612698412698396 1 29 -3.524007936507937 1 30 -4.720059523809526 1 31 -1.8826785714285688 1 32 -1.36215277777778 1 33 -7.048472222222223 1 34 -7.177251984126981 1 35 -5.926617063492065 1 36 -5.727886904761904 1 37 -6.000297619047618 1 38 -5.9057936507936475 1 39 -5.276726190476193 1 40 -5.2312896825396855 2 1 -0.1347023809523762 2 2 -2.117757936507939 2 3 -2.1701884920634953 2 4 -13.781170634920638 2 5 -0.30298611111111384 2 6 -13.769801587301583 2 7 -14.028700396825403 2 8 -2.4080257936507934 2 9 -4.448412698412696 2 10 -12.850119047619042 2 11 -19.867638888888894 2 12 -18.74395833333334 2 13 -19.282351190476188 2 14 -19.4849503968254 2 15 -18.562410714285708 2 16 -16.719742063492063 2 17 -16.622400793650794 2 18 -16.412103174603164 2 19 -13.562807539682534 2 20 -15.086408730158727 2 21 -12.536646825396833 2 22 -12.220099206349204 2 23 -12.673184523809525 2 24 -12.42982142857143 2 25 -12.06114087301587 2 26 -10.61811507936508 2 27 -10.6062996031746 2 28 -9.83873015873016 2 29 -9.324007936507938 2 30 -8.320059523809526 2 31 -7.6826785714285695 2 32 -7.162152777777781 2 33 -7.048472222222223 2 34 -7.177251984126981 2 35 -5.926617063492065 2 36 -5.727886904761904 2 37 -6.000297619047618 2 38 -5.9057936507936475 2 39 -5.276726190476193 2 40 -5.2312896825396855 3 1 0.03196428571428811 3 2 1.3822420634920611 3 3 1.7464781746031726 3 4 -2.781170634920638 3 5 -0.5529861111111138 3 6 -1.2698015873015827 3 7 -5.945367063492071 3 8 -11.658025793650793 3 9 -2.781746031746028 3 10 -5.4334523809523745 3 11 -0.03430555555556225 3 12 -3.243958333333339 3 13 -10.115684523809522 3 14 -7.651617063492067 3 15 -4.39574404761904 3 16 0.28025793650793673 3 17 -5.455734126984128 3 18 -0.24543650793649618 3 19 2.9371924603174655 3 20 1.2469246031746053 3 21 2.130019841269835 3 22 0.9465674603174641 3 23 0.1601488095238075 3 24 1.2368452380952384 3 25 -6.061140873015869 3 26 -3.1181150793650794 3 27 -2.1062996031746 3 28 -2.1720634920634936 3 29 -2.1573412698412717 3 30 -1.320059523809526 3 31 0.6506547619047645 3 32 0.17118055555555323 3 33 -1.0484722222222231 3 34 -2.677251984126981 3 35 -1.0932837301587313 3 36 3.2721130952380957 3 37 0.9997023809523817 3 38 1.9275396825396864 3 39 4.723273809523807 3 40 0.7687103174603145 4 1 -2.434702380952377 4 2 -2.5177579365079374 4 3 -1.1201884920634946 4 4 -2.681170634920637 4 5 -6.652986111111115 4 6 -18.86980158730158 4 7 -23.278700396825403 4 8 -23.158025793650793 4 9 -22.948412698412696 4 10 -20.600119047619042 4 11 -19.867638888888894 4 12 -18.74395833333334 4 13 -19.282351190476188 4 14 -19.4849503968254 4 15 -18.562410714285708 4 16 -16.719742063492063 4 17 -16.622400793650794 4 18 -16.412103174603164 4 19 -13.562807539682534 4 20 -15.086408730158727 4 21 -12.536646825396833 4 22 -12.220099206349204 4 23 -12.673184523809525 4 24 -12.42982142857143 4 25 -12.06114087301587 4 26 -10.61811507936508 4 27 -10.6062996031746 4 28 -9.83873015873016 4 29 -9.324007936507938 4 30 -8.320059523809526 4 31 -7.6826785714285695 4 32 -7.162152777777781 4 33 -7.048472222222223 4 34 -7.177251984126981 4 35 -5.926617063492065 4 36 -5.727886904761904 4 37 -6.000297619047618 4 38 -5.9057936507936475 4 39 -5.276726190476193 4 40 -5.2312896825396855 5 1 -2.134702380952376 5 2 -1.8677579365079389 5 3 -1.4201884920634953 5 4 -12.531170634920638 5 5 -11.552986111111114 5 6 -12.019801587301583 5 7 -18.028700396825403 5 8 -15.908025793650793 5 9 -19.948412698412696 5 10 -13.600119047619042 5 11 -4.117638888888894 5 12 -6.243958333333339 5 13 -3.782351190476188 5 14 -5.2349503968254005 5 15 -1.8124107142857078 5 16 -7.719742063492063 5 17 -4.122400793650794 5 18 -4.912103174603164 5 19 -4.0628075396825345 5 20 -8.836408730158727 5 21 -3.286646825396833 5 22 -6.470099206349204 5 23 -8.423184523809525 5 24 -6.9298214285714295 5 25 -7.561140873015869 5 26 -4.118115079365079 5 27 -5.6062996031746 5 28 -3.5887301587301597 5 29 -3.574007936507938 5 30 -3.070059523809526 5 31 -1.4326785714285695 5 32 -0.9121527777777807 5 33 -4.798472222222223 5 34 -0.6772519841269808 5 35 0.5733829365079348 5 36 -3.4778869047619043 5 37 0.7497023809523817 5 38 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1.2687103174603145 7 1 3.6510119047619085 7 2 5.739384920634915 7 3 -1.6344742063492106 7 4 6.433115079365077 7 5 5.80415674603174 7 6 2.587341269841275 7 7 3.2927281746031696 7 8 2.1276884920634913 7 9 5.337301587301589 7 10 11.257023809523812 7 11 6.7037896825396786 7 12 7.541755952380946 7 13 6.431934523809527 7 14 10.657906746031745 7 15 8.29473214285715 7 16 9.280257936507937 7 17 10.94902777777778 7 18 6.730753968253978 7 19 11.437192460317466 7 20 10.627876984126988 7 21 10.463353174603167 7 22 15.922757936507942 7 23 12.04110119047619 7 24 12.998749999999998 7 25 12.510287698412704 7 26 12.810456349206348 7 27 6.250843253968258 7 28 10.875555555555556 7 29 14.818849206349205 7 30 14.822797619047616 7 31 7.3173214285714305 7 32 6.694990079365077 7 33 1.8086706349206345 7 34 8.679890873015877 7 35 2.7876686507936483 7 36 4.272113095238096 7 37 2.9997023809523817 7 38 3.95134920634921 7 39 2.0089880952380934 7 40 5.054424603174601 8 1 2.865297619047624 8 2 -1.1177579365079389 8 3 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6.468829365079362 9 5 8.197013888888886 9 6 8.480198412698417 9 7 6.221299603174597 9 8 5.841974206349207 9 9 5.176587301587304 9 10 11.399880952380958 9 11 8.757361111111106 9 12 8.256041666666661 9 13 7.092648809523812 9 14 7.7650496031745995 9 15 11.687589285714292 9 16 5.905257936507937 9 17 1.2525992063492062 9 18 1.087896825396836 9 19 5.4371924603174655 9 20 0.5385912698412731 9 21 -2.411646825396833 9 22 -1.9700992063492038 9 23 -3.6731845238095246 9 24 -0.42982142857142946 9 25 -2.1861408730158693 9 26 -4.868115079365079 9 27 -5.7312996031746 9 28 -6.71373015873016 9 29 -6.824007936507938 9 30 -3.070059523809526 9 31 -5.1826785714285695 9 32 -4.162152777777781 9 33 -3.423472222222223 9 34 -4.552251984126981 9 35 -2.301617063492065 9 36 -1.8528869047619043 9 37 -1.7502976190476183 9 38 -2.0307936507936475 9 39 -1.776726190476193 9 40 -1.3562896825396855 10 1 0.2652976190476224 10 2 2.082242063492064 10 3 7.879811507936502 10 4 5.218829365079362 10 5 -1.3529861111111146 10 6 0.9301984126984166 10 7 3.5212996031745973 10 8 7.741974206349205 10 9 3.7515873015873034 10 10 1.999880952380959 10 11 5.532361111111104 10 12 9.456041666666664 10 13 6.217648809523812 10 14 7.715049603174599 10 15 6.337589285714291 10 16 6.480257936507936 10 17 6.877599206349206 10 18 8.087896825396836 10 19 11.337192460317464 10 20 6.913591269841273 10 21 0.8633531746031657 10 22 3.679900793650795 10 23 10.126815476190476 10 24 5.0701785714285705 10 25 1.4388591269841307 10 26 0.6818849206349213 10 27 -1.0062996031746003 10 28 1.5612698412698407 10 29 3.8759920634920615 10 30 1.4799404761904746 10 31 2.3173214285714305 10 32 -0.26215277777778034 10 33 3.5515277777777765 10 34 -1.1772519841269808 10 35 2.8733829365079355 10 36 -2.9278869047619045 10 37 -4.100297619047618 10 38 -2.7057936507936473 10 39 -2.076726190476193 10 40 -2.7312896825396855 11 1 3.165297619047621 11 2 5.082242063492064 11 3 6.479811507936503 11 4 2.518829365079359 11 5 5.347013888888885 11 6 5.93019841269842 11 7 6.521299603174594 11 8 9.441974206349208 11 9 5.851587301587301 11 10 9.399880952380958 11 11 8.332361111111108 11 12 5.4560416666666605 11 13 7.517648809523813 11 14 10.115049603174601 11 15 7.437589285714292 11 16 13.080257936507934 11 17 9.977599206349208 11 18 7.187896825396837 11 19 10.437192460317466 11 20 15.113591269841276 11 21 18.66335317460317 11 22 12.179900793650795 11 23 15.326815476190475 11 24 14.97017857142857 11 25 19.738859126984128 11 26 2.1818849206349213 11 27 3.1937003968254007 11 28 3.961269841269841 11 29 3.2759920634920636 11 30 6.479940476190475 11 31 5.917321428571432 11 32 6.037847222222219 11 33 2.7515277777777776 11 34 7.0227480158730184 11 35 2.6733829365079345 11 36 6.072113095238096 11 37 4.199702380952381 11 38 2.294206349206352 11 39 4.323273809523807 11 40 -2.0312896825396853 12 1 2.615297619047624 12 2 1.2572420634920611 12 3 3.3298115079365047 12 4 5.968829365079362 12 5 5.572013888888886 12 6 7.730198412698417 12 7 1.8462996031745966 12 8 5.591974206349207 12 9 1.801587301587304 12 10 5.774880952380958 12 11 12.132361111111106 12 12 6.881041666666661 12 13 7.467648809523812 12 14 8.3900496031746 12 15 4.312589285714292 12 16 11.155257936507937 12 17 10.127599206349206 12 18 11.462896825396836 12 19 11.437192460317466 12 20 14.288591269841273 12 21 17.838353174603167 12 22 10.029900793650796 12 23 9.326815476190475 12 24 11.32017857142857 12 25 12.93885912698413 12 26 -1.9931150793650794 12 27 -0.8562996031746 12 28 -1.4637301587301597 12 29 -0.8240079365079378 12 30 0.30494047619047393 12 31 -4.4326785714285695 12 32 -7.162152777777781 12 33 -7.048472222222223 12 34 -7.177251984126981 12 35 -5.926617063492065 12 36 -5.727886904761904 12 37 -6.000297619047618 12 38 -5.9057936507936475 12 39 -5.276726190476193 12 40 -5.2312896825396855 13 1 3.222440476190478 13 2 5.882242063492061 13 3 0.6512400793650777 13 4 4.861686507936508 13 5 7.375585317460317 13 6 3.1587698412698444 13 7 3.1498710317460237 13 8 5.556259920634922 13 9 4.051587301587304 13 10 8.971309523809527 13 11 9.98950396825396 13 12 10.541755952380946 13 13 9.289077380952381 13 14 5.800763888888884 13 15 9.437589285714292 13 16 12.708829365079367 13 17 12.949027777777776 13 18 12.016468253968267 13 19 8.008621031746038 13 20 9.3421626984127 13 21 10.03478174603174 13 22 9.779900793650796 13 23 7.041101190476191 13 24 7.1416071428571435 13 25 9.081716269841273 13 26 4.810456349206348 13 27 8.107986111111115 13 28 3.7326984126984133 13 29 10.10456349206349 13 30 3.8227976190476163 13 31 5.8887500000000035 13 32 3.9807043650793617 13 33 3.3800992063492057 13 34 6.108462301587304 13 35 3.073382936507935 13 36 3.7006845238095245 13 37 6.713988095238097 13 38 3.237063492063495 13 39 3.294702380952378 13 40 5.482996031746028 14 1 3.490297619047624 14 2 6.132242063492061 14 3 3.4548115079365047 14 4 7.968829365079362 14 5 7.072013888888886 14 6 8.730198412698417 14 7 9.596299603174597 14 8 9.966974206349207 14 9 5.051587301587304 14 10 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-1.2294742063492023 112 20 6.080257936507941 112 21 0.630019841269835 112 22 4.613234126984128 112 23 1.9934821428571432 112 24 2.5701785714285705 112 25 2.772192460317463 112 26 4.5485515873015885 112 27 7.227033730158732 112 28 4.66126984126984 112 29 3.5093253968253943 112 30 6.013273809523806 112 31 1.8173214285714305 112 32 0.004513888888885376 112 33 -0.7151388888888892 112 34 1.9894146825396852 112 35 -2.926617063492065 112 36 -2.0612202380952374 112 37 -4.166964285714285 112 38 -2.2391269841269805 112 39 -2.276726190476193 112 40 -1.5646230158730186 113 1 2.865297619047624 113 2 5.382242063492061 113 3 2.5798115079365047 113 4 2.968829365079362 113 5 6.697013888888886 113 6 5.730198412698417 113 7 -2.0287003968254034 113 8 -1.6580257936507934 113 9 -2.448412698412696 113 10 5.399880952380958 113 11 -1.6176388888888944 113 12 -2.743958333333339 113 13 -8.282351190476188 113 14 -8.4849503968254 113 15 -7.562410714285708 113 16 -1.4697420634920633 113 17 -5.122400793650794 113 18 -1.912103174603164 113 19 1.1871924603174655 113 20 -6.586408730158727 113 21 -9.036646825396833 113 22 -4.970099206349204 113 23 -5.423184523809525 113 24 -9.92982142857143 113 25 -5.561140873015869 113 26 -9.61811507936508 113 27 -8.8562996031746 113 28 -6.08873015873016 113 29 -5.574007936507938 113 30 -5.570059523809526 113 31 -3.9326785714285695 113 32 -1.4121527777777807 113 33 -1.2984722222222231 113 34 -1.9272519841269808 113 35 -0.6766170634920652 113 36 -2.2278869047619043 113 37 1.9997023809523817 113 38 0.09420634920635251 113 39 2.973273809523807 113 40 0.5187103174603145 114 1 -3.2347023809523776 114 2 3.6822420634920583 114 3 1.6798115079365061 114 4 5.918829365079365 114 5 2.947013888888886 114 6 2.530198412698418 114 7 -1.078700396825404 114 8 7.0419742063492095 114 9 -4.948412698412696 114 10 1.599880952380957 114 11 -2.8676388888888944 114 12 -0.1439583333333374 114 13 -7.882351190476188 114 14 -1.6849503968253998 114 15 -0.9624107142857063 114 16 0.08025793650793744 114 17 -0.4224007936507945 114 18 5.387896825396837 114 19 5.637192460317465 114 20 9.713591269841274 114 21 2.6633531746031665 114 22 0.7799007936507962 114 23 4.926815476190477 114 24 -0.8298214285714298 114 25 -2.2611408730158686 114 26 -3.8181150793650787 114 27 -3.8062996031745993 114 28 -5.23873015873016 114 29 -2.9240079365079374 114 30 -3.320059523809526 114 31 -1.8826785714285688 114 32 -0.7621527777777803 114 33 -1.2484722222222224 114 34 -1.3772519841269801 114 35 -0.12661706349206447 114 36 -0.12788690476190467 114 37 -4.600297619047618 114 38 -0.7057936507936482 114 39 0.7232738095238069 114 40 -0.4312896825396848 115 1 -0.1347023809523762 115 2 -1.951091269841271 115 3 2.2464781746031726 115 4 1.885496031746026 115 5 1.113680555555554 115 6 4.730198412698417 115 7 -4.278700396825403 115 8 -2.4913591269841255 115 9 -9.615079365079364 115 10 -2.4334523809523745 115 11 -8.367638888888894 115 12 -2.4106250000000067 115 13 -1.61568452380952 115 14 -8.151617063492067 115 15 -7.229077380952374 115 16 0.28025793650793673 115 17 -1.6224007936507938 115 18 2.254563492063504 115 19 0.7705257936507977 115 20 -0.7530753968253947 115 21 2.630019841269835 115 22 7.113234126984128 115 23 9.160148809523808 115 24 6.236845238095238 115 25 10.605525793650799 115 26 4.5485515873015885 115 27 3.3937003968254 115 28 7.4946031746031725 115 29 2.3426587301587283 115 30 5.346607142857142 115 31 0.9839880952380966 115 32 0.6711805555555532 115 33 2.784861111111111 115 34 3.156081349206353 115 35 3.9067162698412687 115 36 1.4387797619047618 115 37 4.166369047619048 115 38 1.4275396825396864 115 39 2.056607142857141 115 40 2.1020436507936484 116 1 1.9652976190476252 116 2 -3.7177579365079403 116 3 -0.12018849206349458 116 4 -7.281170634920638 116 5 -5.652986111111115 116 6 -0.6698015873015812 116 7 -9.678700396825402 116 8 -9.558025793650792 116 9 -11.748412698412697 116 10 -7.200119047619044 116 11 -5.867638888888894 116 12 -2.943958333333338 116 13 -3.4823511904761872 116 14 0.3150496031746002 116 15 -5.162410714285709 116 16 -0.9197420634920626 116 17 -1.822400793650793 116 18 -1.2121031746031647 116 19 -5.162807539682534 116 20 -1.8864087301587276 116 21 1.6633531746031665 116 22 0.1799007936507948 116 23 3.5268154761904746 116 24 1.3701785714285712 116 25 -0.8611408730158701 116 26 -1.0181150793650797 116 27 2.3937003968254 116 28 1.1612698412698403 116 29 -1.9240079365079374 116 30 -3.5200595238095254 116 31 0.31732142857143053 116 32 -1.36215277777778 116 33 -0.6484722222222228 116 34 0.6227480158730199 116 35 -2.726617063492065 116 36 -2.527886904761904 116 37 -2.800297619047618 116 38 -1.3057936507936478 116 39 -2.076726190476193 116 40 -0.031289682539686225 117 1 -4.884702380952376 117 2 2.632242063492061 117 3 -2.9201884920634953 117 4 -2.031170634920638 117 5 0.19701388888888616 117 6 3.4801984126984173 117 7 -0.2787003968254034 117 8 -3.4080257936507934 117 9 -5.698412698412696 117 10 2.6498809523809577 117 11 7.132361111111106 117 12 -2.243958333333339 117 13 -0.03235119047618795 117 14 10.5150496031746 117 15 -0.31241071428570777 117 16 -0.46974206349206327 117 17 7.127599206349206 117 18 3.337896825396836 117 19 6.1871924603174655 117 20 2.163591269841273 117 21 1.9633531746031672 117 22 1.7799007936507962 117 23 1.8268154761904754 117 24 2.0701785714285705 117 25 -8.31114087301587 117 26 -7.868115079365079 117 27 -7.8562996031746 117 28 -7.08873015873016 117 29 -6.324007936507938 117 30 -5.570059523809526 117 31 -2.9326785714285695 117 32 -2.4121527777777807 117 33 -5.298472222222223 117 34 -3.427251984126981 117 35 1.3233829365079348 117 36 -5.727886904761904 117 37 -6.000297619047618 117 38 -5.9057936507936475 117 39 -5.276726190476193 117 40 -5.2312896825396855 118 1 1.3652976190476238 118 2 1.8822420634920611 118 3 -3.1201884920634946 118 4 -9.281170634920638 118 5 -3.6529861111111153 118 6 -7.069801587301583 118 7 -8.278700396825403 118 8 -6.958025793650794 118 9 -7.948412698412696 118 10 -12.800119047619042 118 11 -1.0676388888888937 118 12 -2.3439583333333402 118 13 -9.282351190476188 118 14 -9.8849503968254 118 15 -1.9624107142857063 118 16 -3.519742063492064 118 17 -6.4224007936507945 118 18 -2.2121031746031647 118 19 0.037192460317466924 118 20 0.5135912698412746 118 21 7.263353174603168 118 22 6.579900793650797 118 23 3.5268154761904746 118 24 -1.4298214285714295 118 25 6.538859126984132 118 26 13.181884920634921 118 27 11.993700396825401 118 28 1.5612698412698407 118 29 6.475992063492063 118 30 4.879940476190473 118 31 3.91732142857143 118 32 5.037847222222219 118 33 0.15152777777777615 118 34 0.2227480158730195 118 35 6.273382936507934 118 36 5.472113095238095 118 37 6.999702380952382 118 38 5.894206349206353 118 39 5.923273809523806 118 40 7.7687103174603145 119 1 1.3652976190476238 119 2 -2.9177579365079396 119 3 2.0798115079365047 119 4 -0.08117063492063892 119 5 -4.652986111111115 119 6 -2.2698015873015827 119 7 -5.678700396825402 119 8 -1.7580257936507948 119 9 -9.148412698412695 119 10 0.19988095238095838 119 11 -11.267638888888895 119 12 -7.943958333333338 119 13 -11.482351190476187 119 14 -13.284950396825401 119 15 -10.362410714285708 119 16 -11.319742063492063 119 17 -16.622400793650794 119 18 -16.412103174603164 119 19 -13.562807539682534 119 20 -15.086408730158727 119 21 -12.536646825396833 119 22 -12.220099206349204 119 23 -12.673184523809525 119 24 -12.42982142857143 119 25 -12.06114087301587 119 26 -10.61811507936508 119 27 -10.6062996031746 119 28 -9.83873015873016 119 29 -9.324007936507938 119 30 -8.320059523809526 119 31 -7.6826785714285695 119 32 -7.162152777777781 119 33 -7.048472222222223 119 34 -7.177251984126981 119 35 -5.926617063492065 119 36 -5.727886904761904 119 37 -6.000297619047618 119 38 -5.9057936507936475 119 39 -5.276726190476193 119 40 -5.2312896825396855 120 1 2.9652976190476252 120 2 2.882242063492061 120 3 8.879811507936502 120 4 6.318829365079363 120 5 6.547013888888888 120 6 -2.6698015873015812 120 7 -6.478700396825403 120 8 2.241974206349205 120 9 -0.3484126984126945 120 10 -3.400119047619043 120 11 -8.867638888888894 120 12 -4.143958333333337 120 13 -2.4823511904761872 120 14 -9.0849503968254 120 15 -1.3624107142857085 120 16 -2.3197420634920647 120 17 0.1775992063492069 120 18 7.587896825396836 120 19 -1.162807539682536 120 20 -5.886408730158728 120 21 -6.536646825396833 120 22 -9.620099206349204 120 23 -2.6731845238095246 120 24 -5.82982142857143 120 25 -11.06114087301587 120 26 -5.218115079365079 120 27 -9.406299603174599 120 28 -8.43873015873016 120 29 -6.324007936507938 120 30 -5.920059523809526 120 31 -2.48267857142857 120 32 -1.7621527777777803 120 33 -5.848472222222223 120 34 -2.577251984126981 120 35 -2.926617063492065 120 36 -4.527886904761904 120 37 -4.800297619047618 120 38 -1.1057936507936468 120 39 -0.07672619047619378 120 40 0.5687103174603152 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 237.0 5 77.0 6 86.0 7 109.0 8 81.0 9 80.0 10 111.0 11 121.0 12 120.0 13 109.0 14 170.0 15 183.0 16 179.0 17 186.0 18 215.0 19 219.0 20 247.0 21 230.0 22 241.0 23 235.0 24 247.0 25 231.0 26 198.0 27 190.0 28 182.0 29 157.0 30 118.0 31 62.0 32 52.0 33 37.0 34 10.0 35 6.0 36 3.0 37 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 98.03174603174604 0.4444444444444444 1.439153439153439 0.08465608465608467 8 97.75898520084566 0.42283298097251587 1.014799154334038 0.8033826638477801 9 96.40439932318104 1.4805414551607445 1.2690355329949239 0.8460236886632826 10 38.18181818181819 40.23255813953489 8.09725158562368 13.488372093023257 11 24.968287526427062 34.312896405919666 22.15644820295983 18.562367864693446 12 35.56025369978858 18.816067653276956 21.902748414376322 23.72093023255814 13 24.989429175475685 20.80338266384778 26.680761099365753 27.52642706131078 14 24.756871035940804 25.517970401691333 27.061310782241016 22.66384778012685 15 27.653276955602536 22.748414376321353 25.750528541226213 23.847780126849894 16 26.13660393317826 24.973567350391203 27.46880947346162 21.421019242968914 17 29.15433403805497 22.536997885835095 25.073995771670187 23.234672304439748 18 27.27272727272727 22.600422832980975 24.92600422832981 25.200845665961946 19 22.9723991507431 17.8343949044586 20.02123142250531 39.171974522293 20 24.08033826638478 27.33615221987315 25.62367864693446 22.95983086680761 21 25.517970401691333 20.824524312896404 27.885835095137423 25.771670190274843 22 26.617336152219874 18.583509513742072 23.742071881606766 31.05708245243129 23 27.695560253699792 23.488372093023255 25.454545454545453 23.361522198731503 24 24.186046511627907 25.47568710359408 26.723044397463003 23.61522198731501 25 24.920601312724962 25.280542028371798 27.67308913825958 22.125767520643656 26 22.375690607734807 25.286867828304295 30.259243518912026 22.078198045048875 27 22.478325227320788 20.828927891731865 30.196658913089447 26.496087967857896 28 22.741696636344404 23.01671250264438 30.124814893166914 24.116775967844298 29 24.433622697438068 21.003599407156468 29.112852000846917 25.449925894558547 30 20.804232804232804 22.433862433862434 29.26984126984127 27.49206349206349 31 20.406865861411315 24.242424242424242 32.2949777495232 23.055732146641237 32 19.45031712473573 21.374207188160675 34.735729386892174 24.439746300211414 33 17.3784355179704 23.276955602536997 34.84143763213531 24.503171247357294 34 16.004228329809724 22.66384778012685 36.65961945031712 24.672304439746302 35 15.457813491224359 24.085430323535633 37.42863184605625 23.02812433918376 36 15.37322901247621 24.59293719602453 35.01797420173398 25.015859589765277 37 15.050804403048264 24.322607959356475 38.230313293818796 22.396274343776458 38 17.78012684989429 25.031712473572938 36.78646934460888 20.401691331923892 39 17.357293868921776 23.10782241014799 35.85623678646934 23.678646934460886 40 15.940803382663848 26.046511627906977 38.32980972515856 19.682875264270614 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 2.0 16 5.0 17 8.0 18 8.0 19 21.5 20 35.0 21 38.5 22 42.0 23 42.0 24 51.5 25 61.0 26 62.0 27 63.0 28 63.0 29 64.0 30 65.0 31 89.0 32 113.0 33 113.0 34 122.0 35 131.0 36 173.0 37 215.0 38 215.0 39 223.5 40 232.0 41 264.0 42 296.0 43 296.0 44 329.5 45 363.0 46 341.5 47 320.0 48 320.0 49 340.5 50 361.0 51 348.0 52 335.0 53 335.0 54 312.0 55 289.0 56 288.0 57 287.0 58 287.0 59 306.5 60 326.0 61 344.0 62 362.0 63 362.0 64 352.0 65 342.0 66 279.5 67 217.0 68 217.0 69 171.5 70 126.0 71 102.0 72 78.0 73 78.0 74 55.5 75 33.0 76 26.5 77 20.0 78 20.0 79 13.0 80 6.0 81 3.5 82 1.0 83 1.0 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.10570824524312897 8 0.0 9 0.042283298097251586 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.021141649048625793 17 0.0 18 0.0 19 0.42283298097251587 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.14799154334038056 26 0.507399577167019 27 0.021141649048625793 28 0.06342494714587738 29 0.14799154334038056 30 0.10570824524312897 31 0.23255813953488372 32 0.0 33 0.0 34 0.0 35 0.021141649048625793 36 0.021141649048625793 37 0.12684989429175475 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 4730.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.21353065539112 #Duplication Level Percentage of deduplicated Percentage of total 1 95.19165343615332 88.73150105708245 2 3.5835790428668632 6.680761099365751 3 0.7031072805624858 1.9661733615221988 4 0.22680880018144706 0.8456659619450317 5 0.13608528010886822 0.6342494714587738 6 0.04536176003628941 0.2536997885835095 7 0.04536176003628941 0.2959830866807611 8 0.022680880018144705 0.16913319238900634 9 0.022680880018144705 0.19027484143763213 >10 0.022680880018144705 0.23255813953488372 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGTTTGGGGAGTGCAGTGCTGGGTTTGGGACACGCCGGG 11 0.23255813953488372 No Hit GGGTTTGGGGAGTGCAGTGCTGGGTTTGGGACACGCAGGG 9 0.19027484143763213 No Hit GGGTTTGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.16913319238900634 No Hit GGGTTTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT 7 0.14799154334038056 No Hit GGGTTTGGGCTCGTCTTAATCAGCAACATCTTTTGATACA 7 0.14799154334038056 No Hit GGGTTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 6 0.12684989429175475 No Hit GGGTTTGGGAGTGCAGTGCTGGGTTTGGGACACGCAGGGA 6 0.12684989429175475 No Hit GGGTTTGGGTAAATAGACGTCTCCAACTTCTGGAAGAGGA 5 0.10570824524312897 No Hit GGGTTTGGGACTGTACATCTGGATTCTAGTTTGATCTTTC 5 0.10570824524312897 No Hit GGGTTTGGGGAAAAAAAACAAAAAAAAAAAAAAAAAAAAA 5 0.10570824524312897 No Hit GGGTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG 5 0.10570824524312897 No Hit GGGTTTGGGATCACAACATTTCTGTGCTTTCTAGGATGTC 5 0.10570824524312897 No Hit GGGTTTGGGATCAACATTAAGCTCAACTCAGCTTCTATCT 5 0.10570824524312897 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.042283298097251586 0.0 0.0 0.0 0.0 11 0.042283298097251586 0.0 0.0 0.0 0.0 12 0.042283298097251586 0.0 0.0 0.0 0.0 13 0.042283298097251586 0.0 0.0 0.0 0.0 14 0.042283298097251586 0.0 0.0 0.0 0.0 15 0.042283298097251586 0.0 0.0 0.0 0.0 16 0.042283298097251586 0.0 0.0 0.0 0.0 17 0.042283298097251586 0.0 0.0 0.0 0.0 18 0.042283298097251586 0.0 0.0 0.0 0.0 19 0.042283298097251586 0.0 0.0 0.0 0.0 20 0.042283298097251586 0.0 0.0 0.0 0.0 21 0.042283298097251586 0.0 0.0 0.0 0.0 22 0.042283298097251586 0.0 0.0 0.0 0.0 23 0.06342494714587738 0.0 0.0 0.0 0.0 24 0.06342494714587738 0.0 0.0 0.0 0.0 25 0.06342494714587738 0.0 0.0 0.0 0.0 26 0.06342494714587738 0.0 0.0 0.0 0.0 27 0.06342494714587738 0.0 0.0 0.0 0.0 28 0.06342494714587738 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGGGCA 25 1.7511295E-4 33.95745 5 TGGGGAG 25 1.7511295E-4 33.95745 6 TTTGGGT 25 1.7511295E-4 33.95745 4 GGGGAGT 25 1.7511295E-4 33.95745 7 GAAGCAT 20 0.0025326516 33.957447 9 GTATCAA 20 0.0025326516 33.957447 18 TTGGGGT 35 8.250008E-7 33.957447 5 TTGGGGG 45 3.792593E-9 33.957447 5 TGGGGGG 20 0.0025326516 33.957447 6 TTGGGAT 40 5.6130375E-8 33.957447 5 TTGGGAA 80 0.0 33.957447 5 GGAAGCA 35 8.250008E-7 33.957447 8 GTGGTAT 20 0.0025326516 33.957447 15 GGGAAGC 45 3.792593E-9 33.957447 7 GGGAACA 20 0.0025326516 33.957447 7 TGGGAGT 20 0.0025326516 33.957447 6 TGGGAAC 20 0.0025326516 33.957447 6 TGGGAAG 60 1.8189894E-12 33.957443 6 TTTGGGA 235 0.0 32.51245 4 GGGTTTG 490 0.0 32.224926 1 >>END_MODULE