FastQCFastQC Report
Fri 17 Jun 2016
SRR1525820_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525820_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2261
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT140.6191950464396285No Hit
GGGTTTGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT100.4422821760283061No Hit
GGGTTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT80.35382574082264484No Hit
GGGTTTGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.30959752321981426No Hit
GGGTTTGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.26536930561698363No Hit
GGGTTTGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTTT60.26536930561698363No Hit
GGGTTTGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTT60.26536930561698363No Hit
GGGTTTGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT40.17691287041132242No Hit
GGGTTTGGGCCGAGTTTTTTTTTTTTTTTTTTTTTTTTTT40.17691287041132242No Hit
GGGTTTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT30.13268465280849182No Hit
GGGTTTGGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTT30.13268465280849182No Hit
GGGTTTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT30.13268465280849182No Hit
GGGTTTGGGATGTGGATTTTTTTTTTTTTTTTTTTTTTTT30.13268465280849182No Hit
GGGTTTGGGGAGTGCAGTGCTGGGTTTGGGACACGCAGGG30.13268465280849182No Hit
GGGTTTGGGAGTGCAGTGCTGGGTTTGGGAAAAAAAAAAA30.13268465280849182No Hit
GGGTTTGGGCGCGTCGCCTTTTTTTTTTTTTTTTTTTTTT30.13268465280849182No Hit
GGGTTTGGGCGTCGATTTTTTTTTTTTTTTTTTTTTTTTT30.13268465280849182No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGTG200.002068234634.5227285
TTTGGGT355.0952985E-734.5227284
GGGAAGC200.002068234634.5227287
TGGGAAG200.002068234634.5227286
GGGTTTG2500.030.381
GGTTTGG2450.030.2954562
TTGGGAA352.3866278E-529.590915
TTTGGGC352.3866278E-529.590914
GTTTGGG2350.029.3810463
TTTGGGG755.456968E-1227.6181834
TTGGGCC250.006178175627.6181815
TTGGGAG250.006178175627.6181815
TTTGGGA950.027.2547844
Produced by FastQC (version 0.11.5)