FastQCFastQC Report
Fri 17 Jun 2016
SRR1525818_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525818_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60378
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT17952.9729371625426477No Hit
GAGATCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC16502.732783464175693No Hit
GAGATCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC5760.9539898638576966No Hit
GAGATCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG5070.8397098280830767No Hit
GAGATCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT4080.6757428202325351No Hit
GAGATCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC2480.4107456358276193No Hit
GAGATCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC2100.34780880453145185No Hit
GAGATCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1390.2302163039517705No Hit
GAGATCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT1350.22359137434164764No Hit
GAGATCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC1320.21862267713405548No Hit
GAGATCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC1320.21862267713405548No Hit
GAGATCGGGGCTGAGATCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATT1280.21199774752393258No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.21034151512140184No Hit
GAGATCGGGGCTGAGATCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAA1210.2004041207062175No Hit
GAGATCGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC960.15899831064294942No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT880.14574845142270365No Hit
GAGATCGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCA870.1440922190201729No Hit
GAGATCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA860.14243598661764217No Hit
GAGATCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT860.14243598661764217No Hit
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.1358110570075193No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA710.11759250057968133No Hit
GAGATCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC690.1142800357746199No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGATCGGAAGAGCTCGTATGCC680.11262380337208916Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG680.11262380337208916No Hit
GAGATCGGGGCTGAGATCGGGTCACAGATCCTCTTCTGAGCTGAGTTTTTGTTCAA660.10931133856702774No Hit
GAGATCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC640.10599887376196629No Hit
GAGATCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTA640.10599887376196629No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCCC203.9103327E-450.61045538
TCAATTC251.9204614E-550.61045541
CCCCGCA150.007998859550.61045538
TGCTCGT150.00810616250.4403437
GGCGCAT1300.050.44033437
GGGCGCA1350.050.35570536
TGACCGC204.0424432E-450.2713634
ACTGCCC252.0184641E-550.1872942
ACCAACA150.00826911750.1872942
ACATAGC150.00826911750.1872946
ATTCGCC204.0760182E-450.1872944
ATGCTCA150.00826911750.1872945
CCCTCCG204.0760182E-450.1872942
AATCCGT150.00829651250.14536343
TTGACAC150.00829651250.14536343
CGACCGC150.00829651250.14536343
AATTCGC252.028488E-550.1453643
CCCCAAT150.00832397350.10350447
TGATAAC150.00835150350.06171848
TCAGCAA150.008379150.01999733
Produced by FastQC (version 0.11.5)