##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525818_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	60378
Sequences flagged as poor quality	0
Sequence length	56
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.998227831329295	38.0	35.0	40.0	30.0	40.0
2	34.30123554937229	38.0	35.0	39.0	17.0	40.0
3	35.50422339262645	38.0	35.0	39.0	27.0	40.0
4	33.30701580045712	38.0	33.0	39.0	12.0	40.0
5	35.63340952002385	39.0	35.0	40.0	27.0	40.0
6	35.82867932028222	39.0	36.0	40.0	28.0	40.0
7	35.788201000364374	38.0	35.0	39.0	29.0	40.0
8	35.65268806518931	38.0	35.0	39.0	29.0	40.0
9	35.65336712047434	38.0	35.0	40.0	28.0	40.0
10	35.08675345324456	38.0	35.0	39.0	25.0	40.0
11	34.92331643976283	38.0	35.0	39.0	26.0	40.0
12	33.66797509026467	38.0	32.0	39.0	20.0	40.0
13	33.14124349928782	38.0	31.0	39.0	18.0	40.0
14	33.52848719732353	38.0	32.0	39.0	18.0	40.0
15	32.226373844777896	37.0	30.0	39.0	15.0	40.0
16	32.27518301368048	36.0	29.0	39.0	17.0	40.0
17	31.4381562820895	36.0	27.0	39.0	15.0	40.0
18	31.238033720891714	36.0	27.0	39.0	15.0	40.0
19	32.22051078207294	36.0	29.0	39.0	18.0	40.0
20	32.81594289310676	37.0	30.0	39.0	20.0	40.0
21	33.518268243399916	38.0	32.0	39.0	19.0	40.0
22	32.75956474212462	38.0	31.0	39.0	17.0	40.0
23	33.47941303123654	38.0	32.0	39.0	19.0	40.0
24	32.75363543012355	38.0	31.0	39.0	17.0	40.0
25	33.21645301268674	38.0	31.0	39.0	19.0	40.0
26	33.2642684421478	38.0	33.0	39.0	19.0	40.0
27	33.59016529199377	38.0	33.0	39.0	20.0	40.0
28	33.6534002451224	38.0	33.0	39.0	21.0	40.0
29	33.03029249064229	38.0	32.0	39.0	17.0	40.0
30	33.034963066017426	38.0	32.0	39.0	17.0	40.0
31	33.52534035575872	38.0	33.0	39.0	19.0	40.0
32	33.474974328397764	38.0	33.0	39.0	19.0	40.0
33	33.16068766769353	38.0	33.0	39.0	18.0	40.0
34	33.05048196362914	38.0	33.0	39.0	17.0	40.0
35	33.13695385736526	38.0	33.0	39.0	17.0	40.0
36	32.311504190267975	37.0	31.0	39.0	11.0	40.0
37	32.08582596309914	36.0	31.0	39.0	10.0	40.0
38	31.32109377587863	36.0	30.0	38.0	4.0	40.0
39	30.15480804266455	35.0	27.0	38.0	4.0	40.0
40	28.107075424823613	34.0	20.0	38.0	4.0	40.0
41	25.512090496538473	31.0	13.0	37.0	4.0	39.0
42	26.484961409785022	31.0	18.0	37.0	4.0	39.0
43	27.157242704296266	32.0	20.0	38.0	4.0	39.0
44	26.211467753155123	32.0	15.0	38.0	4.0	39.0
45	26.934164761999405	32.0	19.0	38.0	4.0	39.0
46	25.089171552552255	30.0	14.0	36.0	4.0	39.0
47	24.299811189506112	29.0	10.0	36.0	4.0	39.0
48	24.641359435555998	29.0	11.0	36.0	4.0	39.0
49	23.913677167180097	29.0	6.0	36.0	4.0	39.0
50	24.5714001788731	29.0	4.0	36.0	4.0	39.0
51	24.721786081022888	30.0	4.0	36.0	4.0	39.0
52	24.62893769253702	30.0	4.0	36.0	4.0	39.0
53	24.493457882010002	30.0	4.0	36.0	4.0	39.0
54	23.975587134386696	30.0	4.0	36.0	4.0	39.0
55	24.05311537314916	30.0	4.0	36.0	4.0	39.0
56	22.702093477756797	28.0	4.0	36.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	4.615077153213157
1	2	6.03595504832138
1	3	4.7722531459897795
1	4	7.411524577193408
1	5	4.697517682754409
1	6	4.192786135760514
1	7	4.611877915146636
1	8	4.854417162269911
1	9	4.962704614154447
1	10	5.062102621065897
1	11	0.5594333741441417
1	12	-5.465788556412523
1	13	-17.448683548333626
1	14	-5.96022338479035
1	15	1.5127932801219437
1	16	-1.4623990330882606
1	17	-5.7188271839016345
1	18	-3.9208463199918597
1	19	0.17635712079683685
1	20	-0.8743627655193791
1	21	6.050236107861522
1	22	6.736364002499673
1	23	5.736825404431553
1	24	6.905361385497965
1	25	6.100011835539593
1	26	5.684070588429648
1	27	5.381347286778237
1	28	5.102660908835354
1	29	6.070950380690871
1	30	6.09176385727033
1	31	4.959415809040969
1	32	5.195256321099624
1	33	4.536918487583296
1	34	4.7543592314347265
1	35	5.663690263131414
1	36	2.9234958994324174
1	37	2.7329532329626574
1	38	3.9910606907009907
1	39	2.914460861297055
1	40	-11.570075697749436
1	41	-2.543204049519556
1	42	-3.4124304048438177
1	43	-9.209132819044017
1	44	-14.771073181278528
1	45	-3.4964051395988456
1	46	-6.125098425509549
1	47	-11.175972449664151
1	48	-6.352415039596183
1	49	-13.890773163765303
1	50	-4.038292132144491
1	51	0.5480983538092694
1	52	0.38413088308714904
1	53	0.7900707231493733
1	54	1.0585963274009522
1	55	0.5602055323997206
1	56	4.764235880105545
2	1	5.193713516849527
2	2	6.790500502866831
2	3	5.392253145989777
2	4	7.713797304466137
2	5	4.667517682754408
2	6	4.984149772124148
2	7	5.117787006055728
2	8	5.394871707724455
2	9	5.433613705063543
2	10	5.57346625742953
2	11	3.8757970105077746
2	12	0.7251205344965683
2	13	2.0681346334845543
2	14	5.453867524300563
2	15	6.038702371031036
2	16	4.1785100578208265
2	17	7.228445543371091
2	18	4.255517316371776
2	19	6.448175302615024
2	20	5.120182689026073
2	21	6.746599744225158
2	22	7.7759094570451275
2	23	6.8595526771588275
2	24	7.195815930952509
2	25	6.446830017357772
2	26	5.718161497520555
2	27	5.26589274132369
2	28	4.840388181562631
2	29	6.396404926145419
2	30	6.83312749363396
2	31	5.135324899950064
2	32	5.067529048372354
2	33	4.867827578492392
2	34	4.843450140525633
2	35	5.445053899495051
2	36	6.272586808523322
2	37	5.593862323871754
2	38	7.16469705433736
2	39	7.056279043115239
2	40	6.33401521134147
2	41	10.72225049593499
2	42	8.935751413338
2	43	8.445412635501441
2	44	-1.4083459085512544
2	45	9.405413042219337
2	46	6.826719756308634
2	47	4.039027550335849
2	48	6.624403142222
2	49	0.3351359271437886
2	50	6.934889686037327
2	51	9.520371081081997
2	52	9.248676337632602
2	53	8.428707086785735
2	54	9.3340508728555
2	55	9.217478259672447
2	56	9.381054061923727
3	1	5.0315485683959125
3	2	6.673490193588481
3	3	5.26452118722689
3	4	7.534415861167165
3	5	4.952672321929668
3	6	5.111366266969505
3	7	5.193766387499025
3	8	5.153737687105902
3	9	5.017531230836738
3	10	5.733260071862517
3	11	5.389095979579942
3	12	6.055532905630585
3	13	6.816897520082492
3	14	6.336754122238702
3	15	0.44004257721660167
3	16	7.022839954728045
3	17	8.503290904195833
3	18	8.493867831835697
3	19	6.427762931481002
3	20	6.402347637479686
3	21	7.225259538039587
3	22	7.465393993127602
3	23	6.5353258730351165
3	24	7.403135518581376
3	25	6.981572285399007
3	26	6.576408920200969
3	27	5.30754222585977
3	28	5.902450037232732
3	29	6.737538946763973
3	30	6.775807906005099
3	31	4.507799126754186
3	32	4.640312553526993
3	33	5.424219331069711
3	34	4.85159447042254
3	35	5.182785858257937
3	36	6.564545571409923
3	37	7.001697375418139
3	38	8.042841384234265
3	39	8.676175950331732
3	40	10.14185026288786
3	41	13.334724722739114
3	42	11.364514299935937
3	43	11.374278614882883
3	44	11.934643782170394
3	45	12.203454279332739
3	46	12.909812539813789
3	47	12.69645023074822
3	48	13.27656809067561
3	49	13.866373040545849
3	50	12.363652572635264
3	51	12.74830922541189
3	52	12.359294894333633
3	53	12.237469973383675
3	54	12.833432316154468
3	55	12.975622589569355
3	56	12.351981896975278
4	1	4.740968418810311
4	2	6.230696581298208
4	3	4.973233538146644
4	4	7.229483578975941
4	5	4.771047094519112
4	6	4.370816438790811
4	7	4.690532104094942
4	8	4.642518766547987
4	9	4.749692136436089
4	10	5.03425057115502
4	11	4.877169559527381
4	12	6.394728377633825
4	13	6.162056202112005
4	14	6.201318504692715
4	15	6.571839625932995
4	16	7.143215940173771
4	17	8.28295534729266
4	18	8.921399669312951
4	19	7.519940008497375
4	20	6.7321434733397965
4	21	6.593462489323194
4	22	7.10022318253532
4	23	6.519356598727452
4	24	7.316012009383886
4	25	6.4411437428479665
4	26	5.6838477720303615
4	27	5.634912349166825
4	28	5.765094063915569
4	29	6.140914730066985
4	30	6.608029454418279
4	31	5.719442547008889
4	32	5.969097675823331
4	33	5.446455029472784
4	34	5.854626611113865
4	35	6.3074068406715185
4	36	4.538469161464498
4	37	4.681705461126654
4	38	5.438030387670693
4	39	6.542357474487787
4	40	-0.7075534161095085
4	41	10.595387750836952
4	42	9.944967099612509
4	43	9.421883223736735
4	44	9.736948209095804
4	45	8.807962061827183
4	46	11.924366815132164
4	47	12.037262844453494
4	48	12.027932553986702
4	49	12.374155534986922
4	50	11.8950857644687
4	51	11.699390688925131
4	52	11.701421435671815
4	53	11.337530616197505
4	54	12.020717539522163
4	55	11.40022335771166
4	56	11.330857983492358
5	1	5.103713516849524
5	2	6.527734545420028
5	3	4.969061656628078
5	4	7.801031347019329
5	5	5.287943214669305
5	6	4.8424476444645705
5	7	4.785233814566368
5	8	4.982318516235097
5	9	5.245103066765665
5	10	4.903253491472086
5	11	5.413882116890754
5	12	6.311290747262525
5	13	6.453879314335616
5	14	6.4674845455771575
5	15	8.05848960507359
5	16	7.272127079097427
5	17	8.050998734860453
5	18	8.949559869563263
5	19	7.448600834529916
5	20	6.989757157111178
5	21	7.2451103825230305
5	22	7.762717967683425
5	23	6.711680336733291
5	24	7.26198614371847
5	25	7.635553421613093
5	26	6.18092745496736
5	27	5.756105507281134
5	28	5.8195371177328425
5	29	6.494064500613501
5	30	6.3424891957616225
5	31	5.084899368035167
5	32	5.2815716015638365
5	33	4.696976514662605
5	34	5.211322480951168
5	35	4.935692197367388
5	36	6.385778297885025
5	37	6.286628281318556
5	38	6.913207692635233
5	39	7.516491809072683
5	40	9.320398190064875
5	41	12.433101559764776
5	42	11.267453540997575
5	43	10.79860412486314
5	44	11.831441325491301
5	45	10.775838574134234
5	46	11.274804862691614
5	47	12.332006273740106
5	48	11.885254206051783
5	49	12.97407209735655
5	50	11.330421600930944
5	51	10.44824342150753
5	52	10.381229529121963
5	53	10.55530283146659
5	54	10.832774277110818
5	55	11.016201663927767
5	56	9.831692359796069
6	1	4.835672279736123
6	2	6.43637679152663
6	3	4.914005723309366
6	4	7.740601428177474
6	5	4.818651703372964
6	6	4.575283792742702
6	7	4.544281851416557
6	8	4.8650778932914704
6	9	5.02784050918725
6	10	5.197177597635715
6	11	4.883941340404682
6	12	5.560687544805845
6	13	6.868443911835072
6	14	6.120259276877881
6	15	7.522516804020725
6	16	7.383664696996089
6	17	8.204321832030889
6	18	8.55572350193879
6	19	7.757659838697496
6	20	6.7013167096446296
6	21	6.513919331854019
6	22	6.908692962199769
6	23	6.298212470973258
6	24	6.918599436107154
6	25	6.177448574058804
6	26	5.390841909891691
6	27	3.7920783083339984
6	28	5.201419109397676
6	29	4.541662658104183
6	30	5.2397254317782895
6	31	4.0335723226304765
6	32	4.238250697856888
6	33	3.444837887770742
6	34	3.789738800319448
6	35	4.224022971659998
6	36	4.616091963162496
6	37	4.393449952737733
6	38	5.341810456399216
6	39	6.253495537960596
6	40	3.141850262887864
6	41	10.499673176347361
6	42	9.261421516430783
6	43	8.972216759212778
6	44	9.738767493510604
6	45	8.409639846343048
6	46	10.610843467648838
6	47	10.675831674047188
6	48	10.544609327789011
6	49	11.010702937453065
6	50	10.73478659325382
6	51	9.573051493453129
6	52	9.720119636601673
6	53	9.526129767198107
6	54	9.173638501721477
6	55	9.552942177198222
6	56	9.053012824810327
7	1	4.882660885270575
7	2	6.661553134445779
7	3	5.069621567042411
7	4	7.534849936045081
7	5	5.258043998543883
7	6	4.867307666860988
7	7	4.748839637634674
7	8	4.85118749719814
7	9	5.248350547168805
7	10	5.799782046903211
7	11	5.282639115770934
7	12	6.3961731660755134
7	13	6.784450422958237
7	14	6.594920155879507
7	15	7.170807634188925
7	16	7.362720584136621
7	17	8.208445543371095
7	18	8.173412053213884
7	19	7.38185951314134
7	20	6.46702479428923
7	21	6.441862902119894
7	22	6.226435772834602
7	23	6.220078992948299
7	24	7.49950014147883
7	25	6.525251069989356
7	26	5.420793076467923
7	27	5.385892741323687
7	28	5.468809234194204
7	29	5.640089136671737
7	30	5.174180125212914
7	31	4.614798584160589
7	32	3.915950101003933
7	33	4.267301262702919
7	34	4.218713298420369
7	35	5.29347495212663
7	36	6.211534176944376
7	37	6.24228337650333
7	38	7.125749685916304
7	39	7.419963253641555
7	40	7.098225737657263
7	41	11.410145232777094
7	42	10.205751413337996
7	43	9.503307372343542
7	44	10.4806014598698
7	45	10.31436041064039
7	46	11.554088177361269
7	47	12.34376439244111
7	48	11.657034721169367
7	49	12.15145171661747
7	50	11.889100212353117
7	51	11.213528975818836
7	52	11.07551844289576
7	53	10.468180770996263
7	54	10.31720876759234
7	55	10.795372996514551
7	56	9.731580377713204
8	1	4.773926282806968
8	2	6.453266460313639
8	3	4.628636124713182
8	4	7.588265389572516
8	5	4.97943257637143
8	6	4.746702963613508
8	7	4.700127431587646
8	8	4.833382346022333
8	9	5.170634981659283
8	10	5.328785406365704
8	11	4.935158712635435
8	12	6.0134184068369905
8	13	6.2836665483781715
8	14	6.137697311534602
8	15	7.10104279656295
8	16	7.740212185480406
8	17	8.040360436988113
8	18	8.694240720627096
8	19	7.437962536657576
8	20	6.830182689026074
8	21	6.979152935714517
8	22	7.358462648534488
8	23	6.78614842183968
8	24	7.410922313931234
8	25	6.7844895918258565
8	26	6.351140220924812
8	27	5.894403379621565
8	28	6.000388181562627
8	29	6.323851734656056
8	30	6.682914727676518
8	31	4.755112133992618
8	32	4.802848197308524
8	33	4.7076148125349455
8	34	4.519833119249036
8	35	4.871862410133346
8	36	3.907054893629706
8	37	5.05258572812707
8	38	5.923845990507573
8	39	6.8888322346046
8	40	1.5544407432563645
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115	35	2.776905751346902
115	36	2.814068290004805
115	37	2.629417879427308
115	38	2.67877112841143
115	39	4.3092420060782
115	40	5.176978174304434
115	41	6.307806051490548
115	42	4.739084746671331
115	43	3.0417089317977357
115	44	-1.4242718344771816
115	45	3.805783412589708
115	46	-3.434020984432106
115	47	-9.625046523738225
115	48	-2.054856117037261
115	49	-5.674864072856213
115	50	-1.1506658695182317
115	51	-0.5718511411402254
115	52	-0.49465699570072985
115	53	0.09352190160054974
115	54	0.8170138358184609
115	55	0.685626407820596
115	56	2.709942950812618
116	1	4.8103801835161875
116	2	6.680500502866831
116	3	5.002253145989776
116	4	7.433797304466136
116	5	4.794184349421073
116	6	4.764149772124149
116	7	5.007787006055729
116	8	5.098205041057788
116	9	5.153613705063542
116	10	5.273466257429533
116	11	4.359130343841109
116	12	4.7751205344965655
116	13	5.418134633484556
116	14	5.373867524300557
116	15	-4.577964295635635
116	16	3.8918433911541648
116	17	4.981778876704428
116	18	4.2021839830384415
116	19	4.281508635948352
116	20	4.636849355692739
116	21	5.643266410891819
116	22	6.509242790378462
116	23	5.916219343825492
116	24	6.479149264285848
116	25	5.7168300173577755
116	26	5.154828164187222
116	27	5.26589274132369
116	28	4.1670548482292915
116	29	5.339738259478754
116	30	5.073127493633962
116	31	4.518658233283396
116	32	4.240862381705686
116	33	4.55782757849239
116	34	3.540116807192298
116	35	4.231720566161712
116	36	4.939253475189993
116	37	5.420528990538415
116	38	6.158030387670692
116	39	7.546279043115241
116	40	9.214015211341472
116	41	8.572250495934991
116	42	6.939084746671334
116	43	1.1454126355014367
116	44	-1.6583459085512544
116	45	6.362079708886004
116	46	2.4867197563086343
116	47	-4.870972449664151
116	48	0.4310698088886653
116	49	-0.29486407285621397
116	50	1.5248896860373264
116	51	4.937037747748661
116	52	5.16534300429927
116	53	4.2920404201190685
116	54	4.387384206188834
116	55	5.130811593005781
116	56	7.8543873952570635
117	1	3.6723409678299177
117	2	4.926775012670756
117	3	3.5810766754015475
117	4	5.964777696622999
117	5	3.6632039572642157
117	6	3.2433654583986566
117	7	3.8375909276243547
117	8	3.936636413606813
117	9	3.7300842932988374
117	10	3.7597407672334526
117	11	0.0242283830567942
117	12	-5.428801034130885
117	13	-5.965394778280153
117	14	-2.3280932600131656
117	15	-8.702670177988573
117	16	-7.3763919029634835
117	17	0.38099456297893397
117	18	-2.8236983699027327
117	19	-0.6369227366006669
117	20	-0.7482486835229452
117	21	3.7209134697153488
117	22	5.149242790378462
117	23	4.0879840497078455
117	24	4.786600244678002
117	25	3.901928056573457
117	26	4.909337968108787
117	27	4.4486378393629025
117	28	4.18666269136655
117	29	4.562483357517966
117	30	5.333519650496704
117	31	3.8370896058324178
117	32	3.763215322882157
117	33	3.936651107904158
117	34	3.5409011209177876
117	35	4.2191715465538735
117	36	4.597292690876266
117	37	5.269940755244299
117	38	6.359599015121674
117	39	6.954122180370142
117	40	9.35127011330226
117	41	7.095387750836952
117	42	5.85673180549486
117	43	-7.117332462537778
117	44	1.5016540914487457
117	45	4.8569816696703185
117	46	-2.25210377310313
117	47	-3.413717547703367
117	48	-2.7563811715034916
117	49	-2.5670209356013096
117	50	-0.5362867845509101
117	51	2.8366455908859187
117	52	3.61318614155417
117	53	3.0434129691386786
117	54	3.5403253826594216
117	55	2.841399828299899
117	56	6.477916807021767
118	1	4.411405824541831
118	2	5.855885118251447
118	3	4.695330069066699
118	4	7.109951150619981
118	5	4.812902298139022
118	6	4.588765156739534
118	7	4.289325467594189
118	8	4.697948630801378
118	9	4.438229089678927
118	10	5.15038933435261
118	11	1.561181625892388
118	12	-4.0479563885803564
118	13	-4.3534038280539065
118	14	-4.71459401416098
118	15	-13.348220705892043
118	16	-4.909951480640711
118	17	-0.28001599509044794
118	18	-3.2267903759359164
118	19	0.031252225691943636
118	20	-0.16981731097392583
118	21	5.551984359609769
118	22	6.315909457045127
118	23	5.182629600235746
118	24	5.960431315567899
118	25	5.29144540197316
118	26	5.256623035982095
118	27	5.816661972092923
118	28	4.981157412331861
118	29	6.231789541530031
118	30	5.180050570557043
118	31	4.790709515334676
118	32	4.835990586833891
118	33	5.41628911695393
118	34	4.586527063602553
118	35	5.1750538994950475
118	36	5.736432962369477
118	37	6.266170016179444
118	38	6.681620131260438
118	39	8.423202120038319
118	40	9.540938288264552
118	41	8.829173572858068
118	42	6.251905259491846
118	43	1.1492587893475914
118	44	0.2708848606795158
118	45	6.185413042219338
118	46	1.2874889870778645
118	47	1.6221044734127723
118	48	-0.3148276270087713
118	49	2.8828282348360936
118	50	3.001043532191172
118	51	8.188832619543536
118	52	8.011753260709526
118	53	6.894091702170353
118	54	8.142512411317039
118	55	8.325939798133987
118	56	10.167207908077575
119	1	3.759963516849524
119	2	5.631125502866833
119	3	4.0378781459897795
119	4	6.654422304466134
119	5	4.103767682754409
119	6	4.032274772124147
119	7	3.999662006055729
119	8	3.774871707724458
119	9	4.00673870506354
119	10	4.05784125742953
119	11	1.8676720105077749
119	12	-4.703004465503433
119	13	-1.2824903665154466
119	14	-4.16050747569944
119	15	-6.740047628968966
119	16	-4.293364942179171
119	17	1.3834455433710922
119	18	-2.425107683628223
119	19	0.24880030261502029
119	20	-0.34169231097392583
119	21	4.7959747442251555
119	22	6.065909457045127
119	23	4.947052677158826
119	24	3.950815930952512
119	25	4.875580017357773
119	26	4.608786497520555
119	27	4.69526774132369
119	28	4.469138181562627
119	29	5.272654926145417
119	30	5.118752493633963
119	31	3.2378248999500627
119	32	3.9537790483723505
119	33	3.78407757849239
119	34	3.694700140525633
119	35	3.4719288994950475
119	36	5.066961808523324
119	37	4.792612323871751
119	38	5.5734470543373575
119	39	6.7056540431152385
119	40	9.042140211341472
119	41	6.705375495934991
119	42	5.968251413337999
119	43	-3.754587364498562
119	44	-2.0920959085512543
119	45	5.216663042219338
119	46	0.7129697563086346
119	47	-0.20722244966415104
119	48	-4.545596857778001
119	49	0.12201092714378703
119	50	2.4830146860373254
119	51	6.369121081081996
119	52	6.338676337632602
119	53	5.856832086785737
119	54	4.963425872855499
119	55	5.959353259672447
119	56	6.3210540619237285
120	1	3.9069922053741166
120	2	5.715910338932407
120	3	4.407499047629123
120	4	6.890846484794
120	5	4.218009486033097
120	6	4.351690755730708
120	7	4.31139356343278
120	8	4.4265110519867505
120	9	4.2913186230963305
120	10	4.319367896773791
120	11	0.798256026901214
120	12	-4.919961432716548
120	13	-1.7484227435646282
120	14	-1.1966242789781276
120	15	1.3844400759490654
120	16	-6.055916171687368
120	17	0.9100848876333885
120	18	-1.5221876016610096
120	19	0.07538841736911905
120	20	1.461330230009679
120	21	4.002173514716958
120	22	5.488040604586107
120	23	4.583323168962107
120	24	4.481553635870544
120	25	4.337157886210232
120	26	5.017341825389408
120	27	4.717040282307295
120	28	4.828257034021647
120	29	5.251650827784761
120	30	5.435094706748714
120	31	4.753193752409082
120	32	4.350807736896943
120	33	4.4188111850497656
120	34	4.159351779869894
120	35	3.4168571781835695
120	36	5.586685169179063
120	37	5.778780356658636
120	38	5.17027082482916
120	39	7.16595117426278
120	40	9.115654555603765
120	41	-6.37070032373714
120	42	-4.6598223571538036
120	43	-6.221800479252661
120	44	-15.121296728223385
120	45	-3.6875377774527927
120	46	-9.144099915822514
120	47	-6.764087203762511
120	48	-7.352973906958329
120	49	-15.289618171216869
120	50	-5.873798838552839
120	51	-2.7512682631803003
120	52	-2.2678810394165794
120	53	-1.9671945525585244
120	54	-2.2934901107510584
120	55	-1.3887512485242723
120	56	1.6489229143827444
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1759.0
5	36.0
6	147.0
7	149.0
8	225.0
9	289.0
10	207.0
11	156.0
12	155.0
13	142.0
14	163.0
15	226.0
16	286.0
17	364.0
18	457.0
19	577.0
20	746.0
21	893.0
22	1209.0
23	1402.0
24	1518.0
25	1737.0
26	1969.0
27	2087.0
28	2090.0
29	2147.0
30	2326.0
31	2653.0
32	3053.0
33	3477.0
34	3923.0
35	4131.0
36	4475.0
37	5043.0
38	5108.0
39	5052.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.31431978535228	0.48361986153897113	0.12090496538474278	0.08115538772400543
8	99.20003974957766	0.6078372917287754	0.13084235979992712	0.06128059889363676
9	94.53940176885621	1.8135744807711418	2.58869124515552	1.058332505217132
10	33.18427241710557	22.30945046208884	33.288615058465005	11.217662062340587
11	22.412136871045746	24.111431316042268	13.511543939845637	39.964887873066345
12	24.190930471363743	37.223823246878	15.465898174831894	23.119348106926363
13	19.707509357713075	16.244327404021334	16.161515783894796	47.886647454370795
14	18.301368047964488	45.62589022491636	17.87240385570903	18.200337871410117
15	46.597337770382694	17.470881863560734	17.6522462562396	18.27953410981697
16	21.40288663893814	43.19684491358311	19.783916350439956	15.616352097038794
17	21.709458340235216	16.862680139141958	45.54580089448402	15.882060626138811
18	18.536553049123853	16.752790751598265	19.210639636953857	45.50001656232403
19	21.53639662629041	17.029279689804305	18.90337868067408	42.53094500323121
20	19.17624235188778	21.68333084614237	42.07829677162613	17.06213003034373
21	20.40809566398357	16.891914273410848	22.781476696810095	39.91851336579549
22	15.888237437477226	17.421908642220675	45.21514458908874	21.474709331213358
23	16.41988803868959	21.612176620623405	39.860545231706915	22.107390108980095
24	17.12051944610084	22.796991983038495	20.53104087987809	39.55144769098258
25	20.315398886827456	20.264047177312484	40.07586800954147	19.34468592631858
26	44.44812618045661	21.919215348421087	16.03929885019384	17.59335962092846
27	20.13159203155557	42.9430872750174	15.56233219529981	21.362988498127216
28	29.209314651031832	38.253999801252114	17.20328596508662	15.333399582629434
29	35.65540462092186	29.664460698943458	18.437246023452918	16.24288865668176
30	31.41735153764581	21.50881495227996	30.19452545068929	16.879308059384943
31	33.09976974937468	33.52382845500174	18.60888866802498	14.767513127598603
32	33.587343659405285	31.596123581545598	18.818851983765427	15.99768077528369
33	30.94776280234081	31.566121251305518	19.668108950448435	17.81800699590524
34	19.295655491597962	21.712588955426902	45.178574390790104	13.813181162185028
35	19.079601990049753	22.819237147595356	45.33167495854063	12.769485903814262
36	29.909020101751654	23.117842997530783	29.53615166630761	17.436985234409956
37	31.38545725893375	21.079512478235635	33.158112925959706	14.376917336870907
38	35.166932165140054	21.731050046462233	28.295499800876144	14.806517987521573
39	29.676563277492125	22.962348648200365	18.475700779565436	28.88538729474208
40	26.313241749987483	17.77195413043115	29.112487272363087	26.802316847218282
41	23.051958914650875	16.44417678744689	25.588008966509417	34.91585533139282
42	18.812374420883078	34.451437206290166	21.236777868185516	25.49941050464124
43	15.240925845980142	30.43868096835254	31.89486268389068	22.42553050177664
44	16.922309099983362	31.14955914157378	30.833471968058557	21.094659790384295
45	27.79123796880186	23.373713906405577	31.01393959508795	17.821108529704613
46	15.545736923409132	33.44683819743202	22.834410245336613	28.173014633822234
47	15.630929001764137	30.621109742702128	19.545318376992977	34.20264287854076
48	26.55330714794743	25.061734533220637	20.67982565173437	27.705132667097566
49	16.82773737172462	32.43831158048553	19.723689017302647	31.010262030487194
50	18.354209871422647	34.876814599751135	20.26545002073828	26.503525508087932
51	16.379009618099968	36.465722021960495	21.279423246233325	25.87584511370621
52	16.908557116555258	44.09925525386057	23.002537776377117	15.989649853207052
53	16.29026901361674	40.792095649285955	23.84921952839588	19.068415808701427
54	16.00066406574251	26.039677928114884	30.294679173238148	27.664978832904456
55	15.228367380833749	23.979405414382992	33.69706028898854	27.095166915794717
56	17.11681800826707	23.27727883929016	32.742907418781854	26.862995733660917
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	3.0
7	5.0
8	10.5
9	16.0
10	106.0
11	196.0
12	256.0
13	322.0
14	328.0
15	302.5
16	277.0
17	255.5
18	234.0
19	238.0
20	242.0
21	241.0
22	240.0
23	240.0
24	256.0
25	272.0
26	336.5
27	401.0
28	395.5
29	418.0
30	446.0
31	486.5
32	527.0
33	613.0
34	699.0
35	772.5
36	846.0
37	867.5
38	890.5
39	892.0
40	1050.0
41	1208.0
42	1510.0
43	1812.0
44	2447.5
45	3083.0
46	5181.0
47	7802.5
48	8326.0
49	6533.5
50	4741.0
51	4130.5
52	3520.0
53	3406.5
54	3424.5
55	3556.0
56	3301.0
57	3046.0
58	2880.0
59	2714.0
60	2634.5
61	2555.0
62	2285.5
63	1741.5
64	1467.0
65	1365.0
66	1263.0
67	1075.0
68	887.0
69	800.5
70	714.0
71	616.0
72	444.0
73	370.0
74	286.0
75	202.0
76	148.5
77	95.0
78	99.5
79	107.5
80	111.0
81	71.5
82	32.0
83	18.5
84	5.0
85	3.5
86	2.0
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.460432607903541
16	0.05134320447845241
17	0.013249859220245786
18	0.0
19	0.04803073967339097
20	0.1142800357746199
21	0.0
22	0.0
23	0.0
24	0.009937394415184339
25	0.016562324025307232
26	0.033124648050614464
27	0.06624929610122893
28	0.0
29	0.1440922190201729
30	0.043062042465798804
31	0.014906091622776509
32	0.021531021232899402
33	0.09440524694425122
34	0.15734207824041868
35	0.1291861273973964
36	0.057968134088575304
37	0.12090496538474278
38	0.18881049388850243
39	0.14574845142270365
40	0.7767729967869091
41	0.9937394415184339
42	0.2600284871973235
43	0.2517473251846699
44	0.4438702838782338
45	0.19543542349862533
46	0.28984067044287654
47	0.48361986153897113
48	0.0645930636986982
49	0.2583722547947928
50	0.17059193746066448
51	0.29646560005299943
52	0.14740468382523436
53	0.26168471959985423
54	0.2368412335618934
55	0.27824704362516145
56	0.2302163039517705
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	60378.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.4364503627149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.23042405205295	59.281526383782165
2	6.155111809139182	8.178475603696711
3	1.6478448383317128	3.2843088542184242
4	0.8226759404681774	2.1862267713405545
5	0.4487323311644604	1.4906091622776507
6	0.3141126318151223	1.2521116963132268
7	0.2443098247450951	1.136175428136076
8	0.15705631590756114	0.8347411308754844
9	0.12714082716326378	0.7602106727616019
>10	0.7753097499563733	9.127496770346816
>50	0.04238027572108793	2.0206035310874824
>100	0.024929573953581134	2.948093676504687
>500	0.004985914790716226	1.793699691940773
>1k	0.004985914790716226	5.705720626718342
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGATCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	1795	2.9729371625426477	No Hit
GAGATCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	1650	2.732783464175693	No Hit
GAGATCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	576	0.9539898638576966	No Hit
GAGATCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	507	0.8397098280830767	No Hit
GAGATCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	408	0.6757428202325351	No Hit
GAGATCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	248	0.4107456358276193	No Hit
GAGATCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	210	0.34780880453145185	No Hit
GAGATCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	139	0.2302163039517705	No Hit
GAGATCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	135	0.22359137434164764	No Hit
GAGATCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC	132	0.21862267713405548	No Hit
GAGATCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	132	0.21862267713405548	No Hit
GAGATCGGGGCTGAGATCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATT	128	0.21199774752393258	No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	127	0.21034151512140184	No Hit
GAGATCGGGGCTGAGATCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAA	121	0.2004041207062175	No Hit
GAGATCGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	96	0.15899831064294942	No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	88	0.14574845142270365	No Hit
GAGATCGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCA	87	0.1440922190201729	No Hit
GAGATCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	86	0.14243598661764217	No Hit
GAGATCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	86	0.14243598661764217	No Hit
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82	0.1358110570075193	No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	71	0.11759250057968133	No Hit
GAGATCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	69	0.1142800357746199	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGATCGGAAGAGCTCGTATGCC	68	0.11262380337208916	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG	68	0.11262380337208916	No Hit
GAGATCGGGGCTGAGATCGGGTCACAGATCCTCTTCTGAGCTGAGTTTTTGTTCAA	66	0.10931133856702774	No Hit
GAGATCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	64	0.10599887376196629	No Hit
GAGATCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTA	64	0.10599887376196629	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.5415879956275464	0.0	0.0	0.0	0.0
3	0.5415879956275464	0.0	0.0	0.0	0.0
4	0.5415879956275464	0.0	0.0	0.0	0.0
5	0.5415879956275464	0.0	0.0	0.0	0.0
6	0.5415879956275464	0.0	0.0	0.0	0.0
7	0.5415879956275464	0.0	0.0	0.0	0.0
8	0.5415879956275464	0.0	0.0	0.0	0.0
9	0.5415879956275464	0.0	0.0	0.0	0.0
10	0.5449004604326079	0.0	0.0	0.0	0.0
11	0.5465566928351386	0.0	0.0	0.0	0.0
12	0.5498691576402001	0.0	0.0	0.0	0.0
13	0.5581503196528537	0.0	0.0	0.0	0.0
14	0.5813375732882838	0.0	0.0	0.0	0.0
15	0.5846500380933453	0.0	0.0	0.0	0.0
16	0.5879625028984067	0.0	0.0	0.0	0.0
17	0.5945874325085296	0.0	0.0	0.0	0.0
18	0.5945874325085296	0.0	0.0	0.0	0.0
19	0.5962436649110603	0.0	0.0	0.0	0.0
20	0.6078372917287754	0.0	0.0	0.0	0.0
21	0.6227433833515519	0.0	0.0	0.0	0.0
22	0.6359932425717977	0.0	0.0	0.0	0.0
23	0.6426181721819205	0.0	0.0	0.0	0.0
24	0.6624929610122893	0.0	0.0	0.0	0.0
25	0.6724303554274735	0.0	0.0	0.0	0.0
26	0.6724303554274735	0.0	0.0	0.0	0.0
27	0.6823677498426579	0.0	0.0	0.0	0.0
28	0.6956176090629037	0.0	0.0	0.0	0.0
29	0.7005863062704959	0.0	0.0	0.0	0.0
30	0.7022425386730266	0.0	0.0	0.0	0.0
31	0.7171486302958031	0.0	0.0	0.0	0.0
32	0.723773559905926	0.0	0.0	0.0	0.0
33	0.7270860247109875	0.0	0.0	0.0	0.0
34	0.7287422571135181	0.0	0.0	0.0	0.0
35	0.7320547219185796	0.0	0.0	0.0	0.0
36	0.7337109543211103	0.0	0.0	0.0	0.0
37	0.7337109543211103	0.0	0.0	0.0	0.0
38	0.7337109543211103	0.0	0.0	0.0	0.0
39	0.7337109543211103	0.0	0.0	0.0	0.0
40	0.7386796515287025	0.0	0.0	0.0	0.0
41	0.7469608135413561	0.0	0.0	0.0	0.0
42	0.753585743151479	0.0	0.0	0.0	0.0
43	0.7651793699691941	0.0	0.0	0.0	0.0
44	0.7900228560071549	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTCCC	20	3.9103327E-4	50.610455	38
TCAATTC	25	1.9204614E-5	50.610455	41
CCCCGCA	15	0.0079988595	50.610455	38
TGCTCGT	15	0.008106162	50.44034	37
GGCGCAT	130	0.0	50.440334	37
GGGCGCA	135	0.0	50.355705	36
TGACCGC	20	4.0424432E-4	50.27136	34
ACTGCCC	25	2.0184641E-5	50.18729	42
ACCAACA	15	0.008269117	50.18729	42
ACATAGC	15	0.008269117	50.18729	46
ATTCGCC	20	4.0760182E-4	50.18729	44
ATGCTCA	15	0.008269117	50.18729	45
CCCTCCG	20	4.0760182E-4	50.18729	42
AATCCGT	15	0.008296512	50.145363	43
TTGACAC	15	0.008296512	50.145363	43
CGACCGC	15	0.008296512	50.145363	43
AATTCGC	25	2.028488E-5	50.14536	43
CCCCAAT	15	0.008323973	50.103504	47
TGATAAC	15	0.008351503	50.061718	48
TCAGCAA	15	0.0083791	50.019997	33
>>END_MODULE