FastQCFastQC Report
Fri 17 Jun 2016
SRR1525817_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525817_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8105
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAA230.283775447254781No Hit
GAGATCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT190.23442319555829735No Hit
GAGATCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT190.23442319555829735No Hit
GAGATCGGGGACACGCAGGGATGAGATGGGGAAAAAAAAA190.23442319555829735No Hit
GAGATCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAA180.22208513263417642No Hit
GAGATCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT170.20974706971005555No Hit
GAGATCGGGGACACGCAGGGATGAGATGGCCCAAAAAAAA160.19740900678593462No Hit
GAGATCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT150.1850709438618137No Hit
GAGATCGGGGAGTGCAGTGCTGAGATCGGGGACACGCAGG150.1850709438618137No Hit
GAGATCGGGGACACGCAGGGATGAGATGGGAAAAAAAAAA130.16039481801357186No Hit
GAGATCGGGAGTGCAGTGCTGAGATCGGGGACACGCAGGG130.16039481801357186No Hit
GAGATCGGGGAGTGCAGTGCTGAGATCGGGGACACGCCGG120.14805675508945096No Hit
GAGATCGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT110.13571869216533003No Hit
GAGATCGGGCTCGTCTTAATCAGCAACATCTTTTGATACA100.12338062924120913No Hit
GAGATCGGGAGTGCAGTGCTGAGATCGGGGACACGCCGGG100.12338062924120913No Hit
GAGATCGGGGACACGCAGGGATGAGATGGCGAAAAAAAAA100.12338062924120913No Hit
GAGATCGGGGACACGCAGGGATGAGATGGGCCAAAAAAAA90.11104256631708821No Hit
GAGATCGGGGTGCAGTGCTGAGATCGGGGACACGCAGGGA90.11104256631708821No Hit
GAGATCGGGGTGCAGTGCTGAGATCGGGGACACGCCGGGA90.11104256631708821No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGCC200.002595737834.08695614
AGATGGG251.8277828E-434.08695624
GAATAGC200.002595737834.08695613
CAGTGGT251.8277828E-434.08695613
GAAGCAT251.8966038E-433.8765459
GATCGGA503.3469405E-1033.8765453
CGGGCAT251.8966038E-433.8765456
ATCGGAA251.8966038E-433.8765454
CGGGAGA251.8966038E-433.8765456
GGAAGCA552.3646862E-1133.8765458
AAGCATG251.8966038E-433.87654510
AAGCAGT251.8966038E-433.87654510
TCGGGCT251.8966038E-433.8765455
TCGGGCA750.033.8765455
GGGCAGA301.3438095E-533.876547
CGGGGCT200.002676782433.876546
CGGGGCC301.3438095E-533.876546
CGGGGAT200.002676782433.876546
GGCCGAA200.002676782433.876549
GAAGCAG301.3438095E-533.876549
Produced by FastQC (version 0.11.5)