##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525817_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8105
Sequences flagged as poor quality	0
Sequence length	40
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.47674275138803	30.0	25.0	33.0	4.0	36.0
2	18.454410857495372	19.0	10.0	26.0	4.0	32.0
3	20.60703269586675	22.0	12.0	29.0	4.0	34.0
4	21.599629858112277	24.0	12.0	31.0	4.0	35.0
5	21.973103022825416	24.0	14.0	31.0	4.0	35.0
6	16.20715607649599	15.0	11.0	21.0	4.0	33.0
7	22.222085132634177	26.0	12.0	31.0	4.0	35.0
8	22.79086983343615	27.0	12.0	32.0	4.0	35.0
9	21.764096236890808	26.0	10.0	31.0	4.0	34.0
10	18.81739666872301	19.0	7.0	28.0	4.0	33.0
11	20.669833436150526	23.0	8.0	31.0	4.0	35.0
12	20.454780999383097	23.0	4.0	31.0	4.0	35.0
13	21.277729796421962	25.0	4.0	31.0	4.0	35.0
14	21.447378161628624	26.0	4.0	32.0	4.0	35.0
15	20.08895743368291	23.0	4.0	31.0	4.0	35.0
16	19.271190623072176	20.0	4.0	31.0	4.0	35.0
17	20.511165946946328	25.0	4.0	32.0	4.0	36.0
18	20.239605181986427	24.0	4.0	32.0	4.0	36.0
19	17.872177668106108	18.0	4.0	30.0	4.0	34.0
20	19.228994447871685	22.0	4.0	31.0	4.0	35.0
21	16.72029611351018	15.0	4.0	29.0	4.0	34.0
22	16.84478716841456	15.0	4.0	29.0	4.0	34.0
23	17.022825416409624	16.0	4.0	30.0	4.0	34.0
24	16.151634793337447	14.0	4.0	28.0	4.0	33.0
25	16.12942628007403	13.0	4.0	29.0	4.0	33.0
26	14.260579888957434	4.0	4.0	26.0	4.0	33.0
27	14.478716841455892	4.0	4.0	28.0	4.0	34.0
28	14.32054287476866	4.0	4.0	27.0	4.0	33.0
29	13.757433682911783	4.0	4.0	27.0	4.0	33.0
30	12.754595928439235	4.0	4.0	24.0	4.0	33.0
31	12.481061073411475	4.0	4.0	25.0	4.0	33.0
32	11.842442936458976	4.0	4.0	22.0	4.0	32.0
33	11.510672424429364	4.0	4.0	21.0	4.0	31.0
34	11.09586674892042	4.0	4.0	18.0	4.0	31.0
35	10.681184454040716	4.0	4.0	17.0	4.0	31.0
36	10.739913633559532	4.0	4.0	15.0	4.0	33.0
37	10.764836520666256	4.0	4.0	15.0	4.0	33.0
38	10.495249845774213	4.0	4.0	15.0	4.0	32.0
39	9.964096236890809	4.0	4.0	13.0	4.0	32.0
40	9.91523750771129	4.0	4.0	4.0	4.0	32.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	3.476300956823131
1	2	8.520413824357966
1	3	-3.9403618395555053
1	4	8.210988893040966
1	5	8.864497197074517
1	6	5.093124406408595
1	7	6.565263119956747
1	8	-0.9177870806507045
1	9	-2.011525124308431
1	10	1.367362854459838
1	11	-1.252769957855179
1	12	1.8295104191805898
1	13	-4.812102429104552
1	14	1.1388101489018077
1	15	-0.5412395602163578
1	16	-1.0984426031484809
1	17	-3.3976493395605587
1	18	-2.7060850139380648
1	19	-4.721137004711943
1	20	1.201978329803179
1	21	2.6959717422087532
1	22	3.0382685560088003
1	23	2.769364633704207
1	24	0.3134747639190856
1	25	3.195150666820499
1	26	1.9183420585619952
1	27	1.1943365886107262
1	28	-1.4856245714899163
1	29	-0.5032610724414113
1	30	0.2501574352821976
1	31	-0.7289826522202425
1	32	0.11837503112064063
1	33	-1.2379667104090082
1	34	-3.5808422575493335
1	35	-2.7299324476386673
1	36	-3.972607973296906
1	37	-2.383155945472412
1	38	-3.913904024209792
1	39	-2.599399293550965
1	40	-2.92009132521118
3	1	5.776300956823132
3	2	5.520413824357966
3	3	4.409638160444494
3	4	-8.489011106959033
3	5	5.514497197074515
3	6	-0.9568755935914055
3	7	3.715263119956745
3	8	1.3822129193492962
3	9	6.188474875691568
3	10	11.367362854459838
3	11	18.847230042144822
3	12	18.82951041918059
3	13	18.187897570895448
3	14	16.18881014890181
3	15	17.458760439783642
3	16	6.051557396851518
3	17	4.902350660439442
3	18	1.1939149860619338
3	19	9.178862995288057
3	20	3.5019783298031797
3	21	17.095971742208754
3	22	-0.06173144399120112
3	23	19.269364633704207
3	24	14.913474763919085
3	25	0.2951506668205006
3	26	-6.3816579414380055
3	27	15.294336588610726
3	28	16.414375428510084
3	29	8.996738927558589
3	30	1.5743528219758218E-4
3	31	20.971017347779757
3	32	11.21837503112064
3	33	5.912033289590992
3	34	21.669157742450665
3	35	22.370067552361334
3	36	11.427392026703094
3	37	13.116844054527588
3	38	5.686095975790208
3	39	12.300600706449035
3	40	15.07990867478882
6	1	-8.31460813408596
6	2	-7.025040721096579
6	3	-4.135816385010051
6	4	-6.21628383423176
6	5	-5.212775530198211
6	6	-5.5023301390459505
6	7	-11.012009607315981
6	8	-11.708696171559795
6	9	-6.993343306126613
6	10	-3.4508189637219804
6	11	-6.425497230582451
6	12	-5.897762308092137
6	13	-3.0848297018318256
6	14	-4.811189851098192
6	15	-5.268512287489084
6	16	-6.039351694057574
6	17	-4.097649339560558
6	18	-4.442448650301703
6	19	-0.002955186530126497
6	20	-3.225294397469547
6	21	3.1868808331178418
6	22	2.392814010554252
6	23	4.9966373609769335
6	24	3.3680202184645385
6	25	0.8406052122750456
6	26	-1.6543852141652788
6	27	-2.4329361386620008
6	28	-0.7674427533080976
6	29	1.9967389275585887
6	30	-0.2725698374450758
6	31	-0.21080083403842487
6	32	0.12746594021154856
6	33	-1.3606939831362812
6	34	0.12370319699612153
6	35	-1.3572051749113943
6	36	-3.2089716096605425
6	37	-2.428610490926957
6	38	-1.041176751482519
6	39	-3.335762929914601
6	40	-2.2837276888475433
7	1	7.976300956823135
7	2	10.560413824357965
7	3	10.569638160444494
7	4	11.230988893040966
7	5	11.754497197074514
7	6	13.683124406408595
7	7	6.1552631199567465
7	8	2.782212919349295
7	9	5.62847487569157
7	10	10.447362854459836
7	11	9.807230042144823
7	12	6.349510419180589
7	13	9.467897570895449
7	14	10.028810148901808
7	15	11.298760439783642
7	16	6.211557396851518
7	17	6.462350660439441
7	18	1.513914986061934
7	19	3.298862995288058
7	20	2.861978329803179
7	21	4.575971742208752
7	22	9.2182685560088
7	23	5.869364633704208
7	24	8.353474763919086
7	25	5.415150666820502
7	26	4.458342058561994
7	27	3.454336588610726
7	28	5.294375428510083
7	29	5.95673892755859
7	30	3.6001574352821972
7	31	-0.38898265222024264
7	32	-0.7816249688793597
7	33	-0.20796671040900705
7	34	-0.8108422575493339
7	35	-2.5899324476386685
7	36	-3.972607973296906
7	37	-4.083155945472412
7	38	-3.873904024209792
7	39	-3.019399293550965
7	40	-2.72009132521118
9	1	5.276300956823132
9	2	1.0204138243579663
9	3	7.409638160444494
9	4	6.510988893040967
9	5	6.514497197074515
9	6	2.5431244064085945
9	7	9.215263119956745
9	8	9.382212919349296
9	9	7.188474875691568
9	10	10.367362854459838
9	11	14.847230042144822
9	12	11.82951041918059
9	13	15.687897570895448
9	14	11.688810148901808
9	15	9.458760439783642
9	16	9.551557396851518
9	17	8.402350660439442
9	18	8.693914986061934
9	19	7.678862995288057
9	20	7.00197832980318
9	21	-0.9040282577912482
9	22	0.4382685560087989
9	23	-0.7306353662957932
9	24	-1.0865252360809148
9	25	0.2951506668205006
9	26	5.1183420585619945
9	27	4.794336588610726
9	28	3.414375428510084
9	29	4.496738927558589
9	30	5.000157435282198
9	31	2.971017347779757
9	32	4.21837503112064
9	33	2.912033289590992
9	34	5.1691577424506665
9	35	6.370067552361332
9	36	6.927392026703094
9	37	6.116844054527588
9	38	6.186095975790208
9	39	7.8006007064490355
9	40	6.57990867478882
10	1	11.776300956823132
10	2	8.151992771726388
10	3	16.514901318339234
10	4	16.77414678777781
10	5	13.882918249706098
10	6	14.885229669566488
10	7	9.504736804167273
10	8	13.540107656191399
10	9	12.030580138849462
10	10	15.68315232814405
10	11	9.005124778986929
10	12	13.513720945496377
10	13	10.39842388668492
10	14	13.609862780480753
10	15	12.24823412399417
10	16	12.367346870535727
10	17	13.6391927657026
10	18	13.509704459746146
10	19	16.705178784761742
10	20	13.765136224540022
10	21	16.990708584314014
10	22	16.83300539811406
10	23	18.795680423177892
10	24	17.43979055339277
10	25	13.505676982609973
10	26	12.513078900667258
10	27	10.504862904400198
10	28	9.203849112720611
10	29	11.523054717032274
10	30	3.4212100668611445
10	31	4.760491031990284
10	32	4.797322399541692
10	33	5.596243815906783
10	34	4.1428419529769815
10	35	4.738488604992911
10	36	5.5852867635452
10	37	7.537896686106537
10	38	3.94925387052705
10	39	7.3006007064490355
10	40	4.711487622157241
12	1	4.526300956823132
12	2	-3.4795861756420337
12	3	2.1596381604444943
12	4	3.2609888930409667
12	5	6.014497197074515
12	6	1.2931244064085945
12	7	-1.2847368800432548
12	8	4.382212919349296
12	9	-3.0615251243084316
12	10	4.867362854459838
12	11	7.5972300421448224
12	12	0.07951041918058976
12	13	4.687897570895448
12	14	-1.0611898510981916
12	15	6.458760439783642
12	16	9.801557396851518
12	17	11.152350660439442
12	18	6.943914986061934
12	19	9.428862995288057
12	20	15.75197832980318
12	21	17.095971742208754
12	22	14.938268556008799
12	23	18.269364633704207
12	24	17.913474763919083
12	25	15.5451506668205
12	26	-3.3816579414380055
12	27	2.794336588610726
12	28	2.914375428510084
12	29	2.4967389275585887
12	30	6.000157435282198
12	31	-0.5289826522202432
12	32	-0.2816249688793597
12	33	-7.087966710409008
12	34	-7.3308422575493335
12	35	-6.629932447638668
12	36	-6.572607973296906
12	37	-6.883155945472412
12	38	-6.313904024209792
12	39	-5.6993992935509645
12	40	-5.92009132521118
13	1	5.220745401267575
13	2	7.520413824357966
13	3	4.076304827111162
13	4	-3.266788884736812
13	5	6.736719419296737
13	6	5.376457739741927
13	7	4.270818675512302
13	8	6.715546252682628
13	9	6.188474875691568
13	10	9.70069618779317
13	11	11.180563375478155
13	12	11.718399308069479
13	13	11.743453126451005
13	14	9.188810148901808
13	15	9.458760439783642
13	16	15.162668507962632
13	17	9.791239549328331
13	18	12.082803874950823
13	19	10.845529661954725
13	20	15.613089440914294
13	21	12.984860631097641
13	22	9.16049077823102
13	23	11.824920189259764
13	24	12.024585875030196
13	25	7.9618173334871685
13	26	11.951675391895327
13	27	9.294336588610726
13	28	4.969930984065641
13	29	8.552294483114146
13	30	9.666824101948865
13	31	4.859906236668646
13	32	4.773930586676197
13	33	4.912033289590992
13	34	2.446935520228445
13	35	5.592289774583554
13	36	2.9829475822586495
13	37	3.450177387860922
13	38	5.019429309123542
13	39	5.411711817560146
13	40	5.968797563677709
14	1	-0.5570323765102003
14	2	3.5204138243579663
14	3	4.409638160444494
14	4	9.510988893040967
14	5	4.8478305304078475
14	6	6.709791073075262
14	7	12.38192978662341
14	8	1.3822129193492962
14	9	4.855141542358236
14	10	8.70069618779317
14	11	4.180563375478155
14	12	9.82951041918059
14	13	4.52123090422878
14	14	9.188810148901808
14	15	-4.541239560216358
14	16	-1.9484426031484823
14	17	10.235683993772774
14	18	6.527248319395266
14	19	12.178862995288057
14	20	10.50197832980318
14	21	11.429305075542084
14	22	-1.0617314439912011
14	23	-0.7306353662957932
14	24	6.913474763919085
14	25	2.6284840001538328
14	26	-0.38165794143800547
14	27	1.2943365886107259
14	28	0.0810420951767501
14	29	-7.003261072441411
14	30	1.6668241019488637
14	31	-5.362315985553576
14	32	-2.7816249688793597
14	33	2.5786999562576582
14	34	2.0024910757840004
14	35	1.7034008856946663
14	36	4.09405869336976
14	37	3.7835107211942542
14	38	4.019429309123542
14	39	4.9672673731157015
14	40	2.746575341455486
15	1	1.7763009568231318
15	2	-0.47958617564203365
15	3	-5.590361839555506
15	4	-1.4890111069590333
15	5	4.514497197074515
15	6	-2.9568755935914055
15	7	10.715263119956745
15	8	12.382212919349296
15	9	11.188474875691568
15	10	8.367362854459838
15	11	-1.1527699578551776
15	12	15.82951041918059
15	13	17.187897570895448
15	14	17.18881014890181
15	15	18.458760439783642
15	16	17.051557396851518
15	17	17.902350660439442
15	18	15.193914986061934
15	19	20.178862995288057
15	20	16.50197832980318
15	21	17.095971742208754
15	22	5.938268556008799
15	23	8.269364633704207
15	24	12.913474763919085
15	25	18.2951506668205
15	26	2.6183420585619945
15	27	16.294336588610726
15	28	16.414375428510084
15	29	16.99673892755859
15	30	12.000157435282198
15	31	1.9710173477797568
15	32	21.21837503112064
15	33	21.912033289590994
15	34	21.669157742450665
15	35	19.370067552361334
15	36	18.427392026703096
15	37	20.11684405452759
15	38	20.68609597579021
15	39	3.3006007064490355
15	40	21.07990867478882
16	1	3.3763009568231332
16	2	-2.679586175642033
16	3	9.609638160444494
16	4	8.510988893040967
16	5	8.314497197074516
16	6	2.443124406408593
16	7	8.915263119956744
16	8	11.382212919349296
16	9	10.188474875691568
16	10	7.967362854459839
16	11	9.047230042144822
16	12	10.82951041918059
16	13	6.587897570895446
16	14	8.988810148901809
16	15	5.058760439783644
16	16	13.251557396851517
16	17	13.902350660439442
16	18	14.993914986061931
16	19	9.978862995288058
16	20	14.50197832980318
16	21	10.295971742208751
16	22	4.138268556008798
16	23	7.669364633704205
16	24	10.513474763919087
16	25	11.6951506668205
16	26	6.218342058561996
16	27	5.094336588610727
16	28	5.814375428510083
16	29	1.196738927558588
16	30	7.600157435282199
16	31	2.171017347779756
16	32	-1.18162496887936
16	33	3.3120332895909925
16	34	0.8691577424506658
16	35	2.970067552361332
16	36	-0.17260797329690547
16	37	1.9168440545275889
16	38	-1.3139040242097924
16	39	0.9006007064490351
16	40	4.279908674788819
17	1	9.958119138641312
17	2	8.929504733448876
17	3	8.273274524080858
17	4	11.19280707485915
17	5	10.832679015256332
17	6	8.134033497317684
17	7	10.487990392684019
17	8	10.56403110116748
17	9	8.324838512055205
17	10	10.185544672641655
17	11	10.574502769417549
17	12	8.056783146453316
17	13	7.915170298168174
17	14	7.143355603447262
17	15	7.140578621601826
17	16	6.460648305942428
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106	13	-1.0121024291045515
106	14	0.2888101489018098
106	15	0.05876043978364365
106	16	-2.4484426031484823
106	17	0.402350660439442
106	18	-2.5060850139380655
106	19	-5.821137004711943
106	20	-3.6980216701968196
106	21	-6.3040282577912485
106	22	-5.661731443991201
106	23	-5.330635366295793
106	24	-2.986525236080915
106	25	-2.7048493331794994
106	26	-3.781657941438006
106	27	-4.1056634113892745
106	28	-4.085624571489916
106	29	-3.803261072441412
106	30	-3.099842564717802
106	31	-2.7289826522202425
106	32	-3.18162496887936
106	33	-1.8879667104090085
106	34	-3.130842257549334
106	35	-4.029932447638668
106	36	-3.3726079732969056
106	37	-3.783155945472412
106	38	-3.2139040242097927
106	39	-4.399399293550965
106	40	-3.92009132521118
107	1	-4.023699043176869
107	2	-4.079586175642033
107	3	-4.990361839555506
107	4	-5.689011106959033
107	5	-4.485502802925485
107	6	-6.9568755935914055
107	7	-7.284736880043255
107	8	-11.617787080650704
107	9	-5.411525124308431
107	10	-9.232637145540162
107	11	-8.152769957855178
107	12	-7.3704895808194095
107	13	-8.612102429104553
107	14	-8.811189851098192
107	15	-8.341239560216358
107	16	-7.148442603148482
107	17	-11.497649339560558
107	18	-10.406085013938066
107	19	-6.621137004711944
107	20	-5.69802167019682
107	21	-2.704028257791249
107	22	-3.2617314439912004
107	23	-3.530635366295794
107	24	-2.286525236080914
107	25	-1.304849333179499
107	26	-4.3816579414380055
107	27	-4.305663411389274
107	28	-6.785624571489916
107	29	-5.003261072441411
107	30	-3.3998425647178028
107	31	-1.828982652220244
107	32	-2.18162496887936
107	33	-0.8879667104090085
107	34	-1.1308422575493342
107	35	-4.4299324476386674
107	36	-0.37260797329690654
107	37	-1.2831559454724122
107	38	-0.11390402420979306
107	39	-3.099399293550965
107	40	0.2799086747888193
108	1	-8.473699043176868
108	2	-4.604586175642034
108	3	-6.090361839555506
108	4	-6.989011106959033
108	5	-5.735502802925485
108	6	-5.7068755935914055
108	7	-4.659736880043255
108	8	-5.117787080650704
108	9	-3.6865251243084316
108	10	-5.132637145540162
108	11	-6.2777699578551776
108	12	-6.29548958081941
108	13	-3.187102429104552
108	14	-4.436189851098192
108	15	-2.6662395602163578
108	16	-6.573442603148482
108	17	-4.722649339560558
108	18	-8.681085013938066
108	19	-8.696137004711943
108	20	-5.49802167019682
108	21	-3.654028257791248
108	22	-1.4367314439912011
108	23	-3.480635366295793
108	24	-4.336525236080915
108	25	-1.0798493331794994
108	26	-3.1316579414380055
108	27	-5.705663411389274
108	28	-3.835624571489916
108	29	-2.1282610724414113
108	30	-3.6248425647178024
108	31	-1.0289826522202432
108	32	-3.0316249688793597
108	33	0.16203328959099217
108	34	-3.7058422575493335
108	35	-1.2549324476386676
108	36	-2.197607973296906
108	37	-0.00815594547241183
108	38	1.1860959757902076
108	39	-0.44939929355096453
108	40	-1.29509132521118
109	1	-2.223699043176868
109	2	0.5204138243579663
109	3	-0.5903618395555057
109	4	-4.489011106959033
109	5	0.5144971970745154
109	6	0.043124406408594496
109	7	9.715263119956745
109	8	9.382212919349296
109	9	-15.811525124308432
109	10	-12.632637145540162
109	11	-15.152769957855178
109	12	-15.17048958081941
109	13	-15.812102429104552
109	14	-15.811189851098192
109	15	-14.541239560216358
109	16	-13.948442603148482
109	17	-15.097649339560558
109	18	-14.806085013938066
109	19	-12.821137004711943
109	20	-13.49802167019682
109	21	-11.904028257791248
109	22	-11.061731443991201
109	23	-11.730635366295793
109	24	-11.086525236080915
109	25	-10.7048493331795
109	26	-9.381657941438005
109	27	-9.705663411389274
109	28	-9.585624571489916
109	29	-9.003261072441411
109	30	-7.999842564717802
109	31	-8.028982652220243
109	32	-7.78162496887936
109	33	-7.087966710409008
109	34	-7.3308422575493335
109	35	-6.629932447638668
109	36	-6.572607973296906
109	37	-6.883155945472412
109	38	-6.313904024209792
109	39	-5.6993992935509645
109	40	-5.92009132521118
112	1	-2.5570323765102003
112	2	-4.212919508975366
112	3	-4.590361839555506
112	4	-3.089011106959033
112	5	-2.0188361362588196
112	6	-4.490208926924739
112	7	-6.684736880043255
112	8	-7.351120413984036
112	9	-4.411525124308431
112	10	-1.965970478873496
112	11	-4.619436624521844
112	12	-4.970489580819411
112	13	-4.278769095771219
112	14	-3.144523184431524
112	15	-2.941239560216358
112	16	0.251557396851517
112	17	0.369017327106107
112	18	-1.4060850139380676
112	19	-2.0211370047119424
112	20	2.235311663136514
112	21	-0.6373615911245807
112	22	1.804935222675466
112	23	-1.19730203296246
112	24	-2.619858569414248
112	25	-1.5048493331795
112	26	-0.5149912747713383
112	27	-2.838996744722607
112	28	-2.8522912381565835
112	29	-2.7365944057747438
112	30	-3.799842564717803
112	31	-4.562315985553576
112	32	-5.448291635546027
112	33	-4.487966710409008
112	34	-4.6641755908826665
112	35	-3.2299324476386673
112	36	-3.905941306630239
112	37	-3.9498226121390783
112	38	-3.380570690876459
112	39	-3.3660659602176315
112	40	-2.9867579918778464
114	1	-7.723699043176868
114	2	-5.979586175642034
114	3	-5.090361839555506
114	4	-11.489011106959033
114	5	-3.9855028029254846
114	6	-7.4568755935914055
114	7	-3.784736880043255
114	8	-5.617787080650704
114	9	-4.311525124308432
114	10	-7.632637145540162
114	11	-5.6527699578551776
114	12	-5.67048958081941
114	13	-3.812102429104552
114	14	-6.311189851098192
114	15	-3.5412395602163578
114	16	0.5515573968515177
114	17	-1.597649339560558
114	18	-3.306085013938066
114	19	0.6788629952880569
114	20	-13.49802167019682
114	21	-11.904028257791248
114	22	-11.061731443991201
114	23	-11.730635366295793
114	24	-11.086525236080915
114	25	-10.7048493331795
114	26	-9.381657941438005
114	27	-9.705663411389274
114	28	-9.585624571489916
114	29	-9.003261072441411
114	30	-7.999842564717802
114	31	-8.028982652220243
114	32	-7.78162496887936
114	33	-7.087966710409008
114	34	-7.3308422575493335
114	35	-6.629932447638668
114	36	-6.572607973296906
114	37	-6.883155945472412
114	38	-6.313904024209792
114	39	-5.6993992935509645
114	40	-5.92009132521118
116	1	5.776300956823132
116	2	-2.4795861756420337
116	3	-1.5903618395555057
116	4	-11.489011106959033
116	5	3.5144971970745154
116	6	4.0431244064085945
116	7	6.715263119956745
116	8	1.3822129193492962
116	9	9.188474875691568
116	10	-8.632637145540162
116	11	-12.152769957855178
116	12	6.82951041918059
116	13	9.187897570895448
116	14	-3.8111898510981916
116	15	3.4587604397836422
116	16	1.0515573968515177
116	17	-0.09764933956055799
116	18	8.193914986061934
116	19	-10.821137004711943
116	20	-2.4980216701968203
116	21	0.09597174220875182
116	22	0.9382685560087989
116	23	-3.730635366295793
116	24	13.913474763919085
116	25	2.2951506668205006
116	26	6.6183420585619945
116	27	17.294336588610726
116	28	-2.585624571489916
116	29	13.996738927558589
116	30	4.000157435282198
116	31	17.971017347779757
116	32	24.21837503112064
116	33	19.912033289590994
116	34	19.669157742450665
116	35	20.370067552361334
116	36	25.427392026703096
116	37	24.11684405452759
116	38	24.68609597579021
116	39	25.300600706449035
116	40	25.07990867478882
118	1	7.776300956823132
118	2	11.520413824357966
118	3	5.409638160444494
118	4	0.5109888930409667
118	5	-5.485502802925485
118	6	18.043124406408594
118	7	-16.284736880043255
118	8	-16.617787080650704
118	9	-15.811525124308432
118	10	-12.632637145540162
118	11	-15.152769957855178
118	12	-15.17048958081941
118	13	-15.812102429104552
118	14	-15.811189851098192
118	15	-14.541239560216358
118	16	-13.948442603148482
118	17	-15.097649339560558
118	18	-14.806085013938066
118	19	-12.821137004711943
118	20	-13.49802167019682
118	21	-11.904028257791248
118	22	-11.061731443991201
118	23	-11.730635366295793
118	24	-11.086525236080915
118	25	-10.7048493331795
118	26	-9.381657941438005
118	27	-9.705663411389274
118	28	-9.585624571489916
118	29	-9.003261072441411
118	30	-7.999842564717802
118	31	-8.028982652220243
118	32	-7.78162496887936
118	33	-7.087966710409008
118	34	-7.3308422575493335
118	35	-6.629932447638668
118	36	-6.572607973296906
118	37	-6.883155945472412
118	38	-6.313904024209792
118	39	-5.6993992935509645
118	40	-5.92009132521118
119	1	8.776300956823132
119	2	16.520413824357966
119	3	-14.590361839555506
119	4	-14.489011106959033
119	5	-15.485502802925485
119	6	-10.956875593591406
119	7	-16.284736880043255
119	8	-16.617787080650704
119	9	-15.811525124308432
119	10	-12.632637145540162
119	11	-15.152769957855178
119	12	-15.17048958081941
119	13	-15.812102429104552
119	14	-15.811189851098192
119	15	-14.541239560216358
119	16	-13.948442603148482
119	17	-15.097649339560558
119	18	-14.806085013938066
119	19	-12.821137004711943
119	20	-13.49802167019682
119	21	-11.904028257791248
119	22	-11.061731443991201
119	23	-11.730635366295793
119	24	-11.086525236080915
119	25	-10.7048493331795
119	26	-9.381657941438005
119	27	-9.705663411389274
119	28	-9.585624571489916
119	29	-9.003261072441411
119	30	-7.999842564717802
119	31	-8.028982652220243
119	32	-7.78162496887936
119	33	-7.087966710409008
119	34	-7.3308422575493335
119	35	-6.629932447638668
119	36	-6.572607973296906
119	37	-6.883155945472412
119	38	-6.313904024209792
119	39	-5.6993992935509645
119	40	-5.92009132521118
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1519.0
5	91.0
6	91.0
7	92.0
8	77.0
9	106.0
10	128.0
11	198.0
12	247.0
13	263.0
14	281.0
15	296.0
16	282.0
17	317.0
18	354.0
19	335.0
20	381.0
21	405.0
22	431.0
23	409.0
24	381.0
25	328.0
26	301.0
27	230.0
28	179.0
29	135.0
30	108.0
31	68.0
32	42.0
33	18.0
34	7.0
35	4.0
36	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.43174799258801	0.33353922174181594	0.11117974058060531	0.12353304508956146
8	97.4086870681145	0.9378084896347483	0.6169792694965449	1.0365251727541955
9	92.61819528453277	5.122824342673744	1.2961362794716702	0.9628440933218121
10	38.27267119062307	38.4083898827884	10.746452806909316	12.57248611967921
11	24.577421344848858	37.86551511412708	20.357803824799507	17.199259716224553
12	34.84268969771746	17.12523133867983	20.01233806292412	28.019740900678592
13	26.563849475632328	27.72362739049969	21.937075879086983	23.775447254781
14	21.024059222702036	27.871684145589143	20.690931523750773	30.41332510795805
15	36.07649599012955	21.61628624305984	21.789019123997534	20.51819864281308
16	23.3218163869694	22.075518262586378	23.92645607107601	30.676209279368212
17	27.29179518815546	28.63664404688464	21.34484885872918	22.72671190623072
18	33.94201110425663	21.159777914867366	20.592227020357804	24.305983960518198
19	27.138789154389002	15.686517271264083	18.26173084065866	38.912962733688246
20	32.9796421961752	23.92350400987045	21.77668106107341	21.320172732880938
21	24.17026526835287	27.93337446020975	25.206662553979026	22.68969771745836
22	21.99876619370759	19.247378161628625	27.143738433066005	31.610117211597778
23	31.49907464528069	25.083281924737815	23.886489821098085	19.531153608883407
24	21.974090067859343	28.883405305367056	24.20727945712523	24.935225169648366
25	28.118053840454433	21.573227957520373	26.57446283032848	23.734255371696715
26	19.07306163021869	28.367296222664017	28.690357852882702	23.869284294234593
27	19.116376650623227	16.364309514994446	39.49154634086141	25.02776749352092
28	28.315633489750557	17.9056557174611	32.24252901951099	21.536181773277352
29	28.725114381105477	17.361196982811922	32.38530975639916	21.528378879683444
30	20.716934487021014	19.65389369592089	34.635352286773795	24.9938195302843
31	20.460168233547748	21.177634834240475	35.77436912419594	22.587827808015835
32	13.786719328560848	23.43865712169835	38.21278696618119	24.561836583559614
33	12.439837097371344	25.077131926446995	40.41712945822535	22.065901517956313
34	13.300431832202344	25.70018507094386	42.08513263417644	18.91425046267736
35	13.478153542335225	26.215749197728954	40.48383115280178	19.82226610713404
36	12.231547765983708	27.73389286595902	39.97778326339175	20.056776104665513
37	11.163250185230922	27.389478883674983	41.84983946653495	19.59743146455915
38	13.31441263573544	27.085389930898323	41.27591312931885	18.324284304047385
39	14.239881539980257	26.702862783810467	39.72112537018756	19.33613030602172
40	12.055774925962488	29.13376110562685	41.423988153998025	17.386475814412634
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	5.5
15	11.0
16	18.0
17	25.0
18	25.0
19	34.0
20	43.0
21	68.5
22	94.0
23	94.0
24	112.0
25	130.0
26	136.0
27	142.0
28	142.0
29	164.5
30	187.0
31	215.0
32	243.0
33	243.0
34	288.5
35	334.0
36	376.5
37	419.0
38	419.0
39	433.5
40	448.0
41	454.5
42	461.0
43	461.0
44	462.0
45	463.0
46	501.0
47	539.0
48	539.0
49	564.0
50	589.0
51	578.0
52	567.0
53	567.0
54	578.0
55	589.0
56	561.0
57	533.0
58	533.0
59	548.5
60	564.0
61	524.5
62	485.0
63	485.0
64	451.5
65	418.0
66	373.0
67	328.0
68	328.0
69	273.0
70	218.0
71	175.0
72	132.0
73	132.0
74	104.0
75	76.0
76	54.5
77	33.0
78	33.0
79	28.0
80	23.0
81	14.0
82	5.0
83	5.0
84	3.5
85	2.0
86	2.0
87	2.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.12338062924120913
8	0.012338062924120914
9	0.049352251696483655
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.012338062924120914
17	0.0
18	0.0
19	0.3454657618753856
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0863664404688464
26	0.703269586674892
27	0.024676125848241828
28	0.0863664404688464
29	0.22208513263417642
30	0.1850709438618137
31	0.2590993214065392
32	0.03701418877236274
33	0.024676125848241828
34	0.0
35	0.03701418877236274
36	0.03701418877236274
37	0.0863664404688464
38	0.012338062924120914
39	0.012338062924120914
40	0.012338062924120914
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	8105.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.82048118445404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.19087080559706	86.45280690931524
2	3.382692568944437	6.1443553362122145
3	0.6385002037766607	1.7396668723010489
4	0.27170221437304715	0.987045033929673
5	0.12226599646787122	0.5552128315854411
6	0.06792555359326179	0.3701418877236274
7	0.04075533215595707	0.2590993214065392
8	0.02717022143730471	0.19740900678593462
9	0.04075533215595707	0.33312769895126465
>10	0.2173617714984377	2.961135101789019
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGATCGGGGACACGCAGGGATGAGATGGGCAAAAAAAAA	23	0.283775447254781	No Hit
GAGATCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	19	0.23442319555829735	No Hit
GAGATCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.23442319555829735	No Hit
GAGATCGGGGACACGCAGGGATGAGATGGGGAAAAAAAAA	19	0.23442319555829735	No Hit
GAGATCGGGGACACGCAGGGATGAGATGGCCAAAAAAAAA	18	0.22208513263417642	No Hit
GAGATCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	17	0.20974706971005555	No Hit
GAGATCGGGGACACGCAGGGATGAGATGGCCCAAAAAAAA	16	0.19740900678593462	No Hit
GAGATCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	15	0.1850709438618137	No Hit
GAGATCGGGGAGTGCAGTGCTGAGATCGGGGACACGCAGG	15	0.1850709438618137	No Hit
GAGATCGGGGACACGCAGGGATGAGATGGGAAAAAAAAAA	13	0.16039481801357186	No Hit
GAGATCGGGAGTGCAGTGCTGAGATCGGGGACACGCAGGG	13	0.16039481801357186	No Hit
GAGATCGGGGAGTGCAGTGCTGAGATCGGGGACACGCCGG	12	0.14805675508945096	No Hit
GAGATCGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	11	0.13571869216533003	No Hit
GAGATCGGGCTCGTCTTAATCAGCAACATCTTTTGATACA	10	0.12338062924120913	No Hit
GAGATCGGGAGTGCAGTGCTGAGATCGGGGACACGCCGGG	10	0.12338062924120913	No Hit
GAGATCGGGGACACGCAGGGATGAGATGGCGAAAAAAAAA	10	0.12338062924120913	No Hit
GAGATCGGGGACACGCAGGGATGAGATGGGCCAAAAAAAA	9	0.11104256631708821	No Hit
GAGATCGGGGTGCAGTGCTGAGATCGGGGACACGCAGGGA	9	0.11104256631708821	No Hit
GAGATCGGGGTGCAGTGCTGAGATCGGGGACACGCCGGGA	9	0.11104256631708821	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.5552128315854411	0.0	0.0	0.0	0.0
3	0.5552128315854411	0.0	0.0	0.0	0.0
4	0.5552128315854411	0.0	0.0	0.0	0.0
5	0.5552128315854411	0.0	0.0	0.0	0.0
6	0.5552128315854411	0.0	0.0	0.0	0.0
7	0.5552128315854411	0.0	0.0	0.0	0.0
8	0.5552128315854411	0.0	0.0	0.0	0.0
9	0.5552128315854411	0.0	0.0	0.0	0.0
10	0.5552128315854411	0.0	0.0	0.0	0.0
11	0.5552128315854411	0.0	0.0	0.0	0.0
12	0.5552128315854411	0.0	0.0	0.0	0.0
13	0.5552128315854411	0.0	0.0	0.0	0.0
14	0.5552128315854411	0.0	0.0	0.0	0.0
15	0.567550894509562	0.0	0.0	0.0	0.0
16	0.567550894509562	0.0	0.0	0.0	0.0
17	0.567550894509562	0.0	0.0	0.0	0.0
18	0.579888957433683	0.0	0.0	0.0	0.0
19	0.5922270203578038	0.0	0.0	0.0	0.0
20	0.5922270203578038	0.0	0.0	0.0	0.0
21	0.6292412091301666	0.0	0.0	0.0	0.0
22	0.6415792720542874	0.0	0.0	0.0	0.0
23	0.6662553979025293	0.0	0.0	0.0	0.0
24	0.6662553979025293	0.0	0.0	0.0	0.0
25	0.6662553979025293	0.0	0.0	0.0	0.0
26	0.6785934608266502	0.0	0.0	0.0	0.0
27	0.6785934608266502	0.0	0.0	0.0	0.0
28	0.6785934608266502	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATAGCC	20	0.0025957378	34.086956	14
AGATGGG	25	1.8277828E-4	34.086956	24
GAATAGC	20	0.0025957378	34.086956	13
CAGTGGT	25	1.8277828E-4	34.086956	13
GAAGCAT	25	1.8966038E-4	33.876545	9
GATCGGA	50	3.3469405E-10	33.876545	3
CGGGCAT	25	1.8966038E-4	33.876545	6
ATCGGAA	25	1.8966038E-4	33.876545	4
CGGGAGA	25	1.8966038E-4	33.876545	6
GGAAGCA	55	2.3646862E-11	33.876545	8
AAGCATG	25	1.8966038E-4	33.876545	10
AAGCAGT	25	1.8966038E-4	33.876545	10
TCGGGCT	25	1.8966038E-4	33.876545	5
TCGGGCA	75	0.0	33.876545	5
GGGCAGA	30	1.3438095E-5	33.87654	7
CGGGGCT	20	0.0026767824	33.87654	6
CGGGGCC	30	1.3438095E-5	33.87654	6
CGGGGAT	20	0.0026767824	33.87654	6
GGCCGAA	20	0.0026767824	33.87654	9
GAAGCAG	30	1.3438095E-5	33.87654	9
>>END_MODULE