FastQCFastQC Report
Fri 17 Jun 2016
SRR1525816_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525816_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2346
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT100.4262574595055414No Hit
GAGATCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT80.3410059676044331No Hit
GAGATCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT50.2131287297527707No Hit
GAGATCGGGGGCTGGTGAGATGGCCCAGTGGGTAAGAGCA40.17050298380221654No Hit
GAGATCGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.17050298380221654No Hit
GAGATCGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT40.17050298380221654No Hit
GAGATCGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT40.17050298380221654No Hit
GAGATCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.17050298380221654No Hit
GAGATCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC30.1278772378516624No Hit
GAGATCGGGGCCGACTTTTTTTTTTTTTTTTTTTTTTTTT30.1278772378516624No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC30.1278772378516624No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1278772378516624No Hit
GAGATCGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1278772378516624No Hit
GAGATCGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT30.1278772378516624No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTCCGAGC30.1278772378516624No Hit
GAGATCGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT30.1278772378516624No Hit
GAGATCGGGGGGCTGGTGAGATGGCCCAGTGGGTAAGAGC30.1278772378516624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGC557.2759576E-1234.0000044
TCGGGGT309.4076895E-634.05
TCGGGGC200.00225191534.05
TCGGGCC309.4076895E-634.05
TCGGGAT200.00225191534.05
ATCGGGG850.032.04
AGATCGG2400.031.8752
GAGATCG2400.031.8751
GATCGGG2250.031.7333343
ATCGGGA703.6379788E-1229.1428574
TCGGGAG350.001058068224.2857135
Produced by FastQC (version 0.11.5)