##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525816_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2346
Sequences flagged as poor quality	0
Sequence length	40
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	11.656436487638533	4.0	4.0	22.0	4.0	30.0
2	8.343137254901961	4.0	4.0	10.0	4.0	22.0
3	8.135976129582268	4.0	4.0	10.0	4.0	20.0
4	7.578431372549019	4.0	4.0	6.0	4.0	20.0
5	8.401960784313726	4.0	4.0	12.0	4.0	22.0
6	6.132992327365729	4.0	4.0	6.0	4.0	11.0
7	8.312020460358056	4.0	4.0	4.0	4.0	22.0
8	8.946717817561808	4.0	4.0	4.0	4.0	26.0
9	8.634271099744245	4.0	4.0	4.0	4.0	25.0
10	7.592071611253197	4.0	4.0	4.0	4.0	20.0
11	7.771952259164536	4.0	4.0	4.0	4.0	22.0
12	7.669650468883206	4.0	4.0	4.0	4.0	21.0
13	7.581841432225064	4.0	4.0	4.0	4.0	22.0
14	7.331628303495311	4.0	4.0	4.0	4.0	20.0
15	7.179028132992327	4.0	4.0	4.0	4.0	19.0
16	7.890878090366582	4.0	4.0	4.0	4.0	23.0
17	7.3554987212276215	4.0	4.0	4.0	4.0	19.0
18	7.309889173060529	4.0	4.0	4.0	4.0	20.0
19	7.420716112531969	4.0	4.0	4.0	4.0	20.0
20	7.675618073316283	4.0	4.0	4.0	4.0	22.0
21	7.805200341005968	4.0	4.0	4.0	4.0	23.0
22	7.583972719522592	4.0	4.0	4.0	4.0	21.0
23	7.789428815004262	4.0	4.0	4.0	4.0	23.0
24	7.659846547314578	4.0	4.0	4.0	4.0	22.0
25	7.550724637681159	4.0	4.0	4.0	4.0	22.0
26	8.088661551577152	4.0	4.0	4.0	4.0	29.0
27	8.257885763000852	4.0	4.0	4.0	4.0	29.0
28	8.002131287297528	4.0	4.0	4.0	4.0	27.0
29	7.978260869565218	4.0	4.0	4.0	4.0	27.0
30	8.131713554987213	4.0	4.0	4.0	4.0	27.0
31	8.187979539641944	4.0	4.0	4.0	4.0	31.0
32	8.192668371696504	4.0	4.0	4.0	4.0	31.0
33	8.087809036658141	4.0	4.0	4.0	4.0	31.0
34	8.018755328218244	4.0	4.0	4.0	4.0	30.0
35	7.956095481670929	4.0	4.0	4.0	4.0	30.0
36	7.643222506393862	4.0	4.0	4.0	4.0	30.0
37	7.607416879795396	4.0	4.0	4.0	4.0	30.0
38	7.595481670929241	4.0	4.0	4.0	4.0	28.0
39	7.528985507246377	4.0	4.0	4.0	4.0	28.0
40	7.6231884057971016	4.0	4.0	4.0	4.0	30.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	18.060845398765572
1	2	-2.971574614694365
1	3	-3.487513982865872
1	4	-1.1889281470163837
1	5	-2.581604952587096
1	6	-1.8578565138176488
1	7	-5.016928210678211
1	8	-5.268745490620491
1	9	-5.700065708101423
1	10	-3.4403202947845815
1	11	-4.142868738404454
1	12	-3.8548662646876934
1	13	-3.7345115697794276
1	14	-3.381581761492476
1	15	-3.0429550608122025
1	16	-3.9594368429189872
1	17	-3.3679492630385495
1	18	-3.0523062255205122
1	19	-3.6624426664605227
1	20	-3.960930735930736
1	21	-3.484532828282828
1	22	-3.564470598845598
1	23	-3.63326247165533
1	24	-3.960104875283447
1	25	-3.0956980519480526
1	26	-3.9797631931560504
1	27	-4.429013347763348
1	28	-3.881654555761698
1	29	-3.5231009070294794
1	30	-3.9616354875283433
1	31	-3.3332824417645854
1	32	-3.4096932333539467
1	33	-3.234339569160997
1	34	-3.1860988713667284
1	35	-3.148406900639044
1	36	-3.09433879612451
1	37	-2.911104669140383
1	38	-3.15764597505669
1	39	-3.0376153112760234
1	40	-3.1339356575963704
4	1	23.47261010464792
4	2	24.146072444129164
4	3	1.6301330759576569
4	4	8.634601264748323
4	5	25.418395047412904
4	6	14.612731721476468
4	7	16.98307178932179
4	8	9.731254509379509
4	9	21.299934291898577
4	10	-3.4403202947845815
4	11	-4.142868738404454
4	12	-3.8548662646876934
4	13	-3.7345115697794276
4	14	-3.381581761492476
4	15	-3.0429550608122025
4	16	-3.9594368429189872
4	17	-3.3679492630385495
4	18	-3.0523062255205122
4	19	-3.6624426664605227
4	20	-3.960930735930736
4	21	-3.484532828282828
4	22	-3.564470598845598
4	23	-3.63326247165533
4	24	-3.960104875283447
4	25	-3.0956980519480526
4	26	-3.9797631931560504
4	27	-4.429013347763348
4	28	-3.881654555761698
4	29	-3.5231009070294794
4	30	-3.9616354875283433
4	31	-3.3332824417645854
4	32	-3.4096932333539467
4	33	-3.234339569160997
4	34	-3.1860988713667284
4	35	-3.148406900639044
4	36	-3.09433879612451
4	37	-2.911104669140383
4	38	-3.15764597505669
4	39	-3.0376153112760234
4	40	-3.1339356575963704
10	1	1.3614989935368111
10	2	1.9238502219069424
10	3	0.7412441870687676
10	4	3.7457123758594326
10	5	1.3072839363017934
10	6	0.16828727703202429
10	7	3.7608495670995676
10	8	4.175698953823954
10	9	4.077712069676355
10	10	-0.21809807256235914
10	11	0.07935348381776741
10	12	0.5895781797567512
10	13	2.043266207998351
10	14	0.39619601628530177
10	15	2.179267161410019
10	16	1.5961187126365681
10	17	3.187606292517006
10	18	3.9476937744794878
10	19	2.3375573335394773
10	20	0.5946248196248192
10	21	3.4043560606060614
10	22	5.435529401154402
10	23	5.255626417233559
10	24	4.928784013605442
10	25	5.237635281385281
10	26	5.909125695732839
10	27	5.34876443001443
10	28	6.007234333127191
10	29	5.8102324263038545
10	30	5.927253401360546
10	31	3.6667175582354146
10	32	3.5903067666460533
10	33	3.3212159863945585
10	34	3.9250122397443823
10	35	3.4071486549165115
10	36	1.7945500927643794
10	37	1.8666731086373956
10	38	1.9534651360544206
10	39	2.0734957998350874
10	40	2.754953231292519
12	1	6.472610104647922
12	2	12.146072444129164
12	3	13.963466409290989
12	4	12.30126793141499
12	5	12.085061714079572
12	6	5.612731721476469
12	7	13.649738455988457
12	8	13.064587842712841
12	9	13.966600958565245
12	10	12.226346371882087
12	11	11.190464594928878
12	12	12.145133735312307
12	13	11.265488430220572
12	14	5.618418238507524
12	15	8.957044939187798
12	16	15.707229823747682
12	17	14.63205073696145
12	18	5.281027107812822
12	19	16.670890666872808
12	20	13.705735930735932
12	21	7.515467171717172
12	22	8.102196067821069
12	23	6.700070861678004
12	24	6.706561791383219
12	25	5.5709686147186135
12	26	10.353570140177283
12	27	13.23765331890332
12	28	15.118345444238301
12	29	14.47689909297052
12	30	14.705031179138324
12	31	17.333384224902083
12	32	17.59030676664605
12	33	17.765660430839002
12	34	17.48056779529994
12	35	17.518259766027626
12	36	7.572327870542156
12	37	7.755561997526283
12	38	7.175687358276644
12	39	7.629051355390643
12	40	7.1993976757369635
14	1	10.472610104647922
14	2	10.146072444129164
14	3	13.630133075957657
14	4	12.634601264748323
14	5	8.418395047412904
14	6	3.612731721476469
14	7	19.98307178932179
14	8	11.731254509379509
14	9	13.299934291898577
14	10	0.5596797052154185
14	11	18.857131261595548
14	12	19.145133735312307
14	13	19.26548843022057
14	14	15.618418238507523
14	15	19.957044939187796
14	16	15.040563157081014
14	17	4.6320507369614505
14	18	0.9476937744794878
14	19	4.337557333539477
14	20	11.039069264069264
14	21	2.515467171717172
14	22	5.435529401154402
14	23	12.36673752834467
14	24	11.039895124716553
14	25	10.904301948051948
14	26	4.02023680684395
14	27	10.570986652236652
14	28	4.118345444238302
14	29	16.47689909297052
14	30	14.038364512471656
14	31	15.666717558235415
14	32	15.590306766646053
14	33	15.765660430839002
14	34	9.813901128633272
14	35	12.851593099360956
14	36	27.90566120387549
14	37	28.088895330859618
14	38	27.84235402494331
14	39	27.962384688723976
14	40	27.86606434240363
16	1	-1.5273898953520781
16	2	-2.853927555870836
16	3	-0.03653359070900919
16	4	3.301267931414988
16	5	-0.9149382859204298
16	6	-1.053934945190198
16	7	-2.350261544011544
16	8	-2.268745490620491
16	9	-1.0333990414347571
16	10	1.8930130385487525
16	11	-0.47620207173778706
16	12	3.4784670686456405
16	13	0.26548843022057245
16	14	-1.0482484281591429
16	15	2.6237116058544636
16	16	-1.2927701762523203
16	17	-0.7012825963718825
16	18	1.6143604411461538
16	19	3.670890666872811
16	20	0.37240259740259773
16	21	1.1821338383838382
16	22	0.7688627344877359
16	23	2.700070861678004
16	24	-0.29343820861677994
16	25	2.2376352813852813
16	26	6.3535701401772835
16	27	5.904319985569986
16	28	6.451678777571636
16	29	6.8102324263038545
16	30	6.371697845804991
16	31	7.333384224902081
16	32	6.923640099979387
16	33	7.098993764172337
16	34	7.1472344619666055
16	35	6.851593099360956
16	36	7.238994537208824
16	37	7.422228664192951
16	38	7.175687358276644
16	39	7.295718022057311
16	40	7.1993976757369635
17	1	-7.527389895352078
17	2	-4.853927555870836
17	3	-5.369866924042343
17	4	-3.365398735251678
17	5	-5.581604952587096
17	6	-3.387268278523531
17	7	-5.016928210678211
17	8	-5.268745490620491
17	9	-5.700065708101423
17	10	-3.4403202947845815
17	11	-4.142868738404454
17	12	-3.8548662646876934
17	13	-3.7345115697794276
17	14	-3.381581761492476
17	15	-3.0429550608122025
17	16	-3.9594368429189872
17	17	-3.3679492630385495
17	18	-3.0523062255205122
17	19	-3.6624426664605227
17	20	-3.960930735930736
17	21	-3.484532828282828
17	22	-3.564470598845598
17	23	-3.63326247165533
17	24	-3.960104875283447
17	25	-3.0956980519480526
17	26	-3.9797631931560504
17	27	-4.429013347763348
17	28	-3.881654555761698
17	29	-3.5231009070294794
17	30	-3.9616354875283433
17	31	-3.3332824417645854
17	32	-3.4096932333539467
17	33	-3.234339569160997
17	34	-3.1860988713667284
17	35	-3.148406900639044
17	36	-3.09433879612451
17	37	-2.911104669140383
17	38	-3.15764597505669
17	39	-3.0376153112760234
17	40	-3.1339356575963704
19	1	-7.527389895352078
19	2	-4.853927555870836
19	3	-5.369866924042343
19	4	-3.365398735251678
19	5	-5.581604952587096
19	6	-3.387268278523531
19	7	-5.016928210678211
19	8	-5.268745490620491
19	9	-5.700065708101423
19	10	-3.4403202947845815
19	11	-4.142868738404454
19	12	-3.8548662646876934
19	13	-3.7345115697794276
19	14	-3.381581761492476
19	15	-3.0429550608122025
19	16	-3.9594368429189872
19	17	-3.3679492630385495
19	18	-3.0523062255205122
19	19	-3.6624426664605227
19	20	-3.960930735930736
19	21	-3.484532828282828
19	22	-3.564470598845598
19	23	-3.63326247165533
19	24	-3.960104875283447
19	25	-3.0956980519480526
19	26	-3.9797631931560504
19	27	-4.429013347763348
19	28	-3.881654555761698
19	29	-3.5231009070294794
19	30	-3.9616354875283433
19	31	-3.3332824417645854
19	32	-3.4096932333539467
19	33	-3.234339569160997
19	34	-3.1860988713667284
19	35	-3.148406900639044
19	36	-3.09433879612451
19	37	-2.911104669140383
19	38	-3.15764597505669
19	39	-3.0376153112760234
19	40	-3.1339356575963704
24	1	-7.527389895352078
24	2	-4.853927555870836
24	3	-5.369866924042343
24	4	-3.365398735251678
24	5	-5.581604952587096
24	6	-3.387268278523531
24	7	-5.016928210678211
24	8	-5.268745490620491
24	9	-5.700065708101423
24	10	-3.4403202947845815
24	11	-4.142868738404454
24	12	-3.8548662646876934
24	13	-3.7345115697794276
24	14	-3.381581761492476
24	15	-3.0429550608122025
24	16	-3.9594368429189872
24	17	-3.3679492630385495
24	18	-3.0523062255205122
24	19	-3.6624426664605227
24	20	-3.960930735930736
24	21	-3.484532828282828
24	22	-3.564470598845598
24	23	-3.63326247165533
24	24	-3.960104875283447
24	25	-3.0956980519480526
24	26	-3.9797631931560504
24	27	-4.429013347763348
24	28	-3.881654555761698
24	29	-3.5231009070294794
24	30	-3.9616354875283433
24	31	-3.3332824417645854
24	32	-3.4096932333539467
24	33	-3.234339569160997
24	34	-3.1860988713667284
24	35	-3.148406900639044
24	36	-3.09433879612451
24	37	-2.911104669140383
24	38	-3.15764597505669
24	39	-3.0376153112760234
24	40	-3.1339356575963704
25	1	6.13927677131459
25	2	3.479405777462498
25	3	6.296799742624323
25	4	7.301267931414988
25	5	4.08506171407957
25	6	1.9460650548098029
25	7	4.983071789321789
25	8	7.397921176046177
25	9	7.966600958565245
25	10	7.226346371882085
25	11	4.523797928262212
25	12	7.4784670686456405
25	13	9.598821763553904
25	14	10.951751571840855
25	15	4.2903782725211315
25	16	8.040563157081014
25	17	9.965384070294782
25	18	5.614360441146154
25	19	10.004224000206145
25	20	11.039069264069264
25	21	9.515467171717173
25	22	8.102196067821069
25	23	8.36673752834467
25	24	11.039895124716553
25	25	1.9043019480519474
25	26	-1.3130965264893835
25	27	0.9043199855699857
25	28	1.1183454442383018
25	29	1.8102324263038545
25	30	2.3716978458049907
25	31	0.3333842249020815
25	32	0.9236400999793872
25	33	0.7656604308390031
25	34	2.1472344619666055
25	35	2.18492643269429
25	36	7.238994537208824
25	37	7.422228664192951
25	38	7.175687358276644
25	39	7.295718022057311
25	40	7.1993976757369635
26	1	-2.1702470382092205
26	2	-0.7110704130136938
26	3	-0.29843835261377194
26	4	0.9203155504626084
26	5	-2.081604952587096
26	6	-1.387268278523531
26	7	-0.8026424963924974
26	8	-0.19731691919191974
26	9	-0.628637136672852
26	10	0.34539399092970413
26	11	0.14284554730983245
26	12	0.5022765924551642
26	13	1.1940598587920013
26	14	1.6898468099360953
26	15	1.4570449391877975
26	16	3.0405631570810128
26	17	0.9891935941043082
26	18	4.447693774479488
26	19	3.1232716192537637
26	20	3.2533549783549773
26	21	3.87261002886003
26	22	1.7926722582972596
26	23	3.2238803854875275
26	24	2.6113236961451243
26	25	1.4043019480519474
26	26	-0.12262033601319366
26	27	-1.4290133477633482
26	28	-2.238797412904555
26	29	-1.4516723356009082
26	30	-0.6044926303854865
26	31	-0.1904252989074422
26	32	0.8045924809317668
26	33	-0.305768140589568
26	34	0.17104398577612834
26	35	1.4944502422180985
26	36	-0.8800530818387955
26	37	-0.6968189548546686
26	38	-0.9433602607709757
26	39	-0.8233295969903089
26	40	-0.919649943310656
27	1	-1.5273898953520781
27	2	1.1460724441291639
27	3	-0.3698669240423431
27	4	-0.03206540191834506
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109	2	8.312739110795832
109	3	9.630133075957657
109	4	7.301267931414988
109	5	9.085061714079572
109	6	2.779398388143135
109	7	8.483071789321789
109	8	8.564587842712841
109	9	5.466600958565243
109	10	8.05967970521542
109	11	6.69046459492888
109	12	0.14513373531230656
109	13	1.4321550968872385
109	14	3.118418238507524
109	15	4.7903782725211315
109	16	4.707229823747679
109	17	0.46538407029478357
109	18	2.2810271078128217
109	19	0.1708906668728103
109	20	0.8724025974025977
109	21	5.015467171717172
109	22	5.268862734487736
109	23	7.200070861678004
109	24	7.206561791383219
109	25	7.5709686147186135
109	26	10.186903473510618
109	27	9.23765331890332
109	28	8.785012110904969
109	29	10.143565759637188
109	30	8.205031179138324
109	31	7.166717558235415
109	32	11.423640099979385
109	33	11.598993764172334
109	34	10.147234461966605
109	35	6.518259766027622
109	36	0.7389945372088231
109	37	-1.911104669140383
109	38	1.1756873582766438
109	39	-0.5376153112760234
109	40	0.6993976757369627
110	1	-0.5273898953520781
110	2	1.1460724441291639
110	3	-0.3698669240423431
110	4	-2.365398735251678
110	5	-0.5816049525870959
110	6	-2.053934945190198
110	7	0.9830717893217891
110	8	-0.2687454906204909
110	9	-1.0333990414347571
110	10	-2.7736536281179145
110	11	4.19046459492888
110	12	2.1451337353123066
110	13	2.5988217635539064
110	14	1.951751571840858
110	15	0.9570449391877975
110	16	6.373896490414347
110	17	6.298717403628117
110	18	4.614360441146154
110	19	6.004224000206143
110	20	6.372402597402598
110	21	-3.484532828282828
110	22	-3.564470598845598
110	23	-3.63326247165533
110	24	-3.960104875283447
110	25	-3.0956980519480526
110	26	-3.9797631931560504
110	27	-4.429013347763348
110	28	-3.881654555761698
110	29	-3.5231009070294794
110	30	-3.9616354875283433
110	31	-3.3332824417645854
110	32	-3.4096932333539467
110	33	-3.234339569160997
110	34	-3.1860988713667284
110	35	-3.148406900639044
110	36	-3.09433879612451
110	37	-2.911104669140383
110	38	-3.15764597505669
110	39	-3.0376153112760234
110	40	-3.1339356575963704
111	1	4.972610104647922
111	2	4.146072444129164
111	3	1.1301330759576569
111	4	0.6346012647483219
111	5	2.418395047412904
111	6	1.112731721476469
111	7	3.983071789321789
111	8	4.231254509379509
111	9	3.799934291898577
111	10	3.5596797052154185
111	11	3.857131261595546
111	12	5.6451337353123066
111	13	2.2654884302205724
111	14	8.118418238507523
111	15	-0.04295506081220246
111	16	8.040563157081014
111	17	-0.8679492630385495
111	18	-2.0523062255205122
111	19	-0.6624426664605227
111	20	2.539069264069264
111	21	-1.9845328282828278
111	22	-1.064470598845598
111	23	4.36673752834467
111	24	7.539895124716553
111	25	2.9043019480519474
111	26	11.52023680684395
111	27	11.070986652236652
111	28	11.618345444238301
111	29	11.97689909297052
111	30	11.538364512471656
111	31	9.166717558235415
111	32	5.590306766646053
111	33	4.265660430839003
111	34	5.813901128633272
111	35	9.351593099360956
111	36	10.40566120387549
111	37	10.588895330859618
111	38	10.34235402494331
111	39	10.462384688723976
111	40	10.366064342403629
116	1	21.47261010464792
116	2	24.146072444129164
116	3	20.63013307595766
116	4	3.634601264748322
116	5	9.418395047412904
116	6	13.612731721476468
116	7	4.983071789321789
116	8	2.731254509379509
116	9	21.299934291898577
116	10	18.55967970521542
116	11	24.857131261595548
116	12	16.145133735312307
116	13	22.26548843022057
116	14	19.618418238507523
116	15	13.957044939187798
116	16	15.040563157081014
116	17	13.63205073696145
116	18	13.947693774479488
116	19	23.33755733353948
116	20	9.039069264069264
116	21	-1.4845328282828278
116	22	8.435529401154401
116	23	-2.63326247165533
116	24	10.039895124716553
116	25	1.9043019480519474
116	26	22.02023680684395
116	27	24.570986652236652
116	28	22.1183454442383
116	29	-3.5231009070294794
116	30	-3.9616354875283433
116	31	-3.3332824417645854
116	32	-3.4096932333539467
116	33	-3.234339569160997
116	34	-3.1860988713667284
116	35	-3.148406900639044
116	36	-3.09433879612451
116	37	-2.911104669140383
116	38	-3.15764597505669
116	39	-3.0376153112760234
116	40	-3.1339356575963704
118	1	-7.527389895352078
118	2	-4.853927555870836
118	3	-5.369866924042343
118	4	-3.365398735251678
118	5	-5.581604952587096
118	6	-3.387268278523531
118	7	-5.016928210678211
118	8	-5.268745490620491
118	9	-5.700065708101423
118	10	-3.4403202947845815
118	11	-4.142868738404454
118	12	-3.8548662646876934
118	13	-3.7345115697794276
118	14	-3.381581761492476
118	15	-3.0429550608122025
118	16	-3.9594368429189872
118	17	-3.3679492630385495
118	18	-3.0523062255205122
118	19	-3.6624426664605227
118	20	-3.960930735930736
118	21	-3.484532828282828
118	22	-3.564470598845598
118	23	-3.63326247165533
118	24	-3.960104875283447
118	25	-3.0956980519480526
118	26	-3.9797631931560504
118	27	-4.429013347763348
118	28	-3.881654555761698
118	29	-3.5231009070294794
118	30	-3.9616354875283433
118	31	-3.3332824417645854
118	32	-3.4096932333539467
118	33	-3.234339569160997
118	34	-3.1860988713667284
118	35	-3.148406900639044
118	36	-3.09433879612451
118	37	-2.911104669140383
118	38	-3.15764597505669
118	39	-3.0376153112760234
118	40	-3.1339356575963704
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1707.0
5	19.0
6	22.0
7	25.0
8	16.0
9	15.0
10	22.0
11	19.0
12	24.0
13	29.0
14	14.0
15	19.0
16	13.0
17	18.0
18	23.0
19	31.0
20	54.0
21	58.0
22	61.0
23	42.0
24	38.0
25	32.0
26	18.0
27	14.0
28	6.0
29	4.0
30	2.0
31	0.0
32	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	97.65258215962442	0.6828851899274435	0.6828851899274435	0.9816474605207
8	96.88832054560955	1.3640238704177323	0.5967604433077579	1.1508951406649617
9	94.19795221843003	2.6023890784982933	1.6638225255972698	1.5358361774744027
10	42.58312020460358	30.562659846547312	10.3154305200341	16.538789428815004
11	30.73316283034953	25.831202046035806	23.273657289002557	20.161977834612106
12	35.97612958226769	14.578005115089516	23.785166240409207	25.66069906223359
13	25.66069906223359	19.99147485080989	26.38533674339301	27.96248934356351
14	19.01918976545842	21.279317697228144	31.769722814498934	27.931769722814497
15	30.647911338448424	18.32907075873828	28.72975277067349	22.293265132139812
16	28.09036658141518	20.971867007672635	27.791986359761296	23.145780051150894
17	24.296675191815854	22.08013640238704	29.283887468030688	24.33930093776641
18	24.254049445865302	25.31969309462916	27.578857630008525	22.847399829497018
19	20.744225834046194	21.899059024807528	28.186484174508124	29.17023096663815
20	23.572037510656436	24.722932651321397	28.47399829497016	23.231031543052
21	17.94543904518329	26.726342710997443	31.415174765558397	23.91304347826087
22	16.197783461210573	25.95907928388747	34.14322250639386	23.6999147485081
23	21.961620469083158	19.31769722814499	32.40938166311301	26.311300639658846
24	19.607843137254903	26.598465473145783	30.008525149190113	23.785166240409207
25	20.308483290488432	18.55184233076264	27.977720651242503	33.16195372750643
26	14.822725331055105	20.162323793250746	30.24348568987612	34.77146518581803
27	15.289079229122054	18.115631691648822	35.07494646680942	31.5203426124197
28	18.458244111349035	21.370449678800856	29.850107066381153	30.321199143468952
29	19.811724433033802	20.196833547282843	32.43474540008558	27.556696619597776
30	18.00341296928328	19.283276450511945	36.04948805460751	26.66382252559727
31	17.44880546075085	17.619453924914676	37.45733788395905	27.474402730375424
32	19.01918976545842	20.213219616204693	35.60767590618337	25.15991471215352
33	15.09594882729211	17.270788912579956	40.3411513859275	27.292110874200425
34	12.65984654731458	20.076726342710998	44.84228473998295	22.421142369991475
35	10.618336886993603	21.023454157782517	42.72921108742004	25.62899786780384
36	11.993171148100727	24.242424242424242	41.69867690994452	22.065727699530516
37	14.524421593830333	24.29305912596401	40.65981148243359	20.522707797772068
38	15.607675906183369	23.49680170575693	39.872068230277186	21.023454157782517
39	13.299232736572892	21.14236999147485	40.32395566922421	25.234441602728047
40	13.341858482523444	19.30946291560102	38.53367433930094	28.815004262574597
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	3.5
15	6.0
16	11.5
17	17.0
18	17.0
19	24.5
20	32.0
21	41.0
22	50.0
23	50.0
24	58.0
25	66.0
26	65.0
27	64.0
28	64.0
29	74.5
30	85.0
31	76.0
32	67.0
33	67.0
34	73.0
35	79.0
36	89.0
37	99.0
38	99.0
39	96.0
40	93.0
41	94.0
42	95.0
43	95.0
44	110.0
45	125.0
46	125.0
47	125.0
48	125.0
49	138.5
50	152.0
51	151.5
52	151.0
53	151.0
54	143.5
55	136.0
56	136.0
57	136.0
58	136.0
59	139.5
60	143.0
61	145.0
62	147.0
63	147.0
64	133.0
65	119.0
66	119.0
67	119.0
68	119.0
69	106.0
70	93.0
71	74.0
72	55.0
73	55.0
74	46.5
75	38.0
76	31.5
77	25.0
78	25.0
79	18.5
80	12.0
81	10.5
82	9.0
83	9.0
84	7.5
85	6.0
86	3.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.1278772378516624
8	0.0
9	0.08525149190110827
10	0.0
11	0.0
12	0.0
13	0.0
14	0.042625745950554135
15	0.0
16	0.0
17	0.0
18	0.0
19	0.3410059676044331
20	0.0
21	0.0
22	0.0
23	0.042625745950554135
24	0.0
25	0.5115089514066496
26	0.2131287297527707
27	0.4688832054560955
28	0.4688832054560955
29	0.3836317135549872
30	0.08525149190110827
31	0.08525149190110827
32	0.042625745950554135
33	0.042625745950554135
34	0.0
35	0.042625745950554135
36	0.1278772378516624
37	0.5115089514066496
38	0.042625745950554135
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	2346.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.88832054560955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.37219533655961	95.31116794543905
2	0.8798944126704795	1.7050298380221656
3	0.39595248570171576	1.1508951406649617
4	0.21997360316761988	0.8525149190110828
5	0.04399472063352398	0.2131287297527707
6	0.0	0.0
7	0.0	0.0
8	0.04399472063352398	0.3410059676044331
9	0.0	0.0
>10	0.04399472063352398	0.4262574595055414
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGATCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	10	0.4262574595055414	No Hit
GAGATCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.3410059676044331	No Hit
GAGATCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	5	0.2131287297527707	No Hit
GAGATCGGGGGCTGGTGAGATGGCCCAGTGGGTAAGAGCA	4	0.17050298380221654	No Hit
GAGATCGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.17050298380221654	No Hit
GAGATCGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT	4	0.17050298380221654	No Hit
GAGATCGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.17050298380221654	No Hit
GAGATCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.17050298380221654	No Hit
GAGATCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC	3	0.1278772378516624	No Hit
GAGATCGGGGCCGACTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.1278772378516624	No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	3	0.1278772378516624	No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.1278772378516624	No Hit
GAGATCGGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.1278772378516624	No Hit
GAGATCGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.1278772378516624	No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTCCGAGC	3	0.1278772378516624	No Hit
GAGATCGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	3	0.1278772378516624	No Hit
GAGATCGGGGGGCTGGTGAGATGGCCCAGTGGGTAAGAGC	3	0.1278772378516624	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.1278772378516624	0.0	0.0	0.0	0.0
3	0.1278772378516624	0.0	0.0	0.0	0.0
4	0.1278772378516624	0.0	0.0	0.0	0.0
5	0.1278772378516624	0.0	0.0	0.0	0.0
6	0.1278772378516624	0.0	0.0	0.0	0.0
7	0.1278772378516624	0.0	0.0	0.0	0.0
8	0.1278772378516624	0.0	0.0	0.0	0.0
9	0.1278772378516624	0.0	0.0	0.0	0.0
10	0.1278772378516624	0.0	0.0	0.0	0.0
11	0.1278772378516624	0.0	0.0	0.0	0.0
12	0.1278772378516624	0.0	0.0	0.0	0.0
13	0.1278772378516624	0.0	0.0	0.0	0.0
14	0.1278772378516624	0.0	0.0	0.0	0.0
15	0.1278772378516624	0.0	0.0	0.0	0.0
16	0.1278772378516624	0.0	0.0	0.0	0.0
17	0.1278772378516624	0.0	0.0	0.0	0.0
18	0.1278772378516624	0.0	0.0	0.0	0.0
19	0.1278772378516624	0.0	0.0	0.0	0.0
20	0.1278772378516624	0.0	0.0	0.0	0.0
21	0.1278772378516624	0.0	0.0	0.0	0.0
22	0.1278772378516624	0.0	0.0	0.0	0.0
23	0.1278772378516624	0.0	0.0	0.0	0.0
24	0.1278772378516624	0.0	0.0	0.0	0.0
25	0.1278772378516624	0.0	0.0	0.0	0.0
26	0.1278772378516624	0.0	0.0	0.0	0.0
27	0.1278772378516624	0.0	0.0	0.0	0.0
28	0.1278772378516624	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGGC	55	7.2759576E-12	34.000004	4
TCGGGGT	30	9.4076895E-6	34.0	5
TCGGGGC	20	0.002251915	34.0	5
TCGGGCC	30	9.4076895E-6	34.0	5
TCGGGAT	20	0.002251915	34.0	5
ATCGGGG	85	0.0	32.0	4
AGATCGG	240	0.0	31.875	2
GAGATCG	240	0.0	31.875	1
GATCGGG	225	0.0	31.733334	3
ATCGGGA	70	3.6379788E-12	29.142857	4
TCGGGAG	35	0.0010580682	24.285713	5
>>END_MODULE