SRR1525815_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1525815_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2564
Sequences flagged as poor quality	0
Sequence length	40
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAGATCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG	114	4.446177847113884	No Hit
GAGATCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT	91	3.5491419656786274	No Hit
GAGATCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT	25	0.9750390015600624	No Hit
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22	0.858034321372855	No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	21	0.8190327613104524	No Hit
GAGATCGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG	18	0.702028081123245	No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	14	0.5460218408736349	No Hit
GAGATCGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG	14	0.5460218408736349	No Hit
GAGATCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13	0.5070202808112325	No Hit
GAGATCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	13	0.5070202808112325	No Hit
GAGATCGGGGCTGAGATCGGGTCACAGATCCTCTTCTGAA	11	0.4290171606864275	No Hit
GAGATCGGGGTGCAGTGCTGAGATCGGGTCACAGATCCTC	8	0.31201248049922	No Hit
GAGATCGGGGCTGAGATCGGGTCACAGATCCTCTTCTGAG	8	0.31201248049922	No Hit
GAGATCGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT	8	0.31201248049922	No Hit
GAGATCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT	8	0.31201248049922	No Hit
GAGATCGGGTCACAGATCCTCTTCTGAGATGAGGATCGGA	6	0.234009360374415	No Hit
GAGATCGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT	6	0.234009360374415	No Hit
GAGATCGGGTCAAAGATCCTATTCTGAAGGAGATTGGGCG	6	0.234009360374415	No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT	5	0.19500780031201248	No Hit
GAGATCGGGCGGATTGAACAAAAACTCATCTCAGAAGAGG	5	0.19500780031201248	No Hit
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	5	0.19500780031201248	No Hit
GAGATCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.19500780031201248	No Hit
GAGATCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.19500780031201248	No Hit
GAGATCGGGCCACAGATCCTCTTCTGAAGGAGATTGGGCG	4	0.15600624024961	No Hit
GAGATCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	4	0.15600624024961	No Hit
GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGATCG	4	0.15600624024961	Illumina Single End Adapter 2 (100% over 34bp)
GAGATCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT	4	0.15600624024961	No Hit
GAGATCGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGG	4	0.15600624024961	No Hit
GAGATCGGGTCACAGATCCTCTTAAGGAGATTGGGCGGAT	4	0.15600624024961	No Hit
GAGATCGGGAGCCTACACCTGTTCTAAGCCAATCAGCAAA	4	0.15600624024961	No Hit
GAGATCGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4	0.15600624024961	No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA	4	0.15600624024961	No Hit
GAGATCGGGTCACAGATAATCTTCTGAAGGAGATTGGGCG	4	0.15600624024961	No Hit
GAGATCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4	0.15600624024961	No Hit
GAGATCGGGGAGTGCAGTGCTGAGATCGGGGAAAAAAAAA	4	0.15600624024961	No Hit
GAGATCGGGGCTTCTTGTATGATCATGGGCATCTCTTTCT	3	0.1170046801872075	No Hit
GAGATCGGGTCCTCTTCTGAAGGAGATTGGGCGGATTGAA	3	0.1170046801872075	No Hit
GAGATCGGGCTGAGATCGGGTCACAGATCCTCTTCTGAAG	3	0.1170046801872075	No Hit
GAGATCGGGTCACAGATCATCTTCTGAAGGAGATTGGGCG	3	0.1170046801872075	No Hit
GAGATCGGGTCAAAGATACTCTTCTGAGATGAGTTTTTGT	3	0.1170046801872075	No Hit
GAGATCGGGGAGGAGAAGCCCGGTTTACGCTGATGACCGA	3	0.1170046801872075	No Hit
GAGATCGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG	3	0.1170046801872075	No Hit
GAGATCGGGCGGATTGAACAACAACTCATCTCAGAAGAGG	3	0.1170046801872075	No Hit
GAGATCGGGTCACAGATTCTCTTCTGAAGGAGATTGGGCG	3	0.1170046801872075	No Hit
GAGATCGGCTCACAGATCCTCTTCTGAAGGAGATTGGGCG	3	0.1170046801872075	No Hit
GAGATCGGGGCTGGGCAGTGGTGGCACATGCCTTTAATCC	3	0.1170046801872075	No Hit
GAGATCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGC	3	0.1170046801872075	No Hit
GAGATCGGATCACAGATCCTCTTCTGAGATGAGTTTTTGT	3	0.1170046801872075	No Hit
GAGATCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT	3	0.1170046801872075	No Hit
GAGATCGAGTCACAGATCCTCTTCTGAGATGAGTTTTTGT	3	0.1170046801872075	No Hit
GAGATCGGGCGGATTGAAAAAAAACTCATCTCAGAAGAGG	3	0.1170046801872075	No Hit
GAGATCGGCAGTGATTGATGTGTTTGCTATGCCACAGTCA	3	0.1170046801872075	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTGAA	25	1.5435615E-4	33.901962	22
ATCGGGC	30	1.0057232E-5	33.901962	4
ATCGGGA	70	0.0	33.901962	4
TCGGGGT	20	0.002334186	33.901962	5
TCGGGGG	40	4.0665327E-8	33.901962	5
TCGGGGC	20	0.002334186	33.901962	5
TCGGGAG	30	1.0057232E-5	33.901962	5
TCGGGAA	30	1.0057232E-5	33.901962	5
GAGATCG	265	0.0	32.622643	1
ATCGGGG	90	0.0	32.01852	4
AGATCGG	260	0.0	31.94608	2
GATCGGG	245	0.0	31.826332	3
TCACAGA	45	1.1483644E-7	30.135077	10
CACAGAT	45	1.1483644E-7	30.135077	11
CTTCTGA	45	1.1483644E-7	30.135077	21
GTCACAG	40	1.8360224E-6	29.664215	9
ACAGATC	40	1.8360224E-6	29.664215	12
CTCTTCT	40	1.8360224E-6	29.664215	19
GGTCACA	40	1.8360224E-6	29.664215	8
CAGATCC	40	1.8360224E-6	29.664215	13

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content