##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525815_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2564 Sequences flagged as poor quality 0 Sequence length 40 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 18.279251170046802 25.0 4.0 30.0 4.0 33.0 2 11.391965678627145 4.0 4.0 19.0 4.0 28.0 3 11.985959438377535 4.0 4.0 20.0 4.0 28.0 4 9.508580343213728 4.0 4.0 10.0 4.0 25.0 5 12.771840873634945 4.0 4.0 23.0 4.0 30.0 6 12.519110764430577 4.0 4.0 23.0 4.0 30.0 7 12.881435257410297 4.0 4.0 25.0 4.0 31.0 8 13.10569422776911 4.0 4.0 26.0 4.0 31.0 9 12.664976599063962 4.0 4.0 25.0 4.0 31.0 10 12.090873634945398 4.0 4.0 23.0 4.0 31.0 11 12.24609984399376 4.0 4.0 23.0 4.0 33.0 12 11.900936037441499 4.0 4.0 23.0 4.0 32.0 13 11.240639625585024 4.0 4.0 20.0 4.0 31.0 14 10.912636505460219 4.0 4.0 17.0 4.0 31.0 15 11.283151326053042 4.0 4.0 20.0 4.0 32.0 16 9.209048361934478 4.0 4.0 10.0 4.0 26.0 17 9.015990639625585 4.0 4.0 10.0 4.0 26.0 18 8.786661466458659 4.0 4.0 10.0 4.0 25.0 19 8.796801872074884 4.0 4.0 10.0 4.0 26.0 20 9.019110764430577 4.0 4.0 10.0 4.0 27.0 21 8.839703588143525 4.0 4.0 6.0 4.0 26.0 22 9.057722308892355 4.0 4.0 4.0 4.0 28.0 23 8.97191887675507 4.0 4.0 4.0 4.0 27.0 24 8.461388455538222 4.0 4.0 4.0 4.0 25.0 25 8.753510140405616 4.0 4.0 4.0 4.0 27.0 26 8.813182527301093 4.0 4.0 4.0 4.0 27.0 27 8.598673946957879 4.0 4.0 4.0 4.0 26.0 28 9.05226209048362 4.0 4.0 4.0 4.0 28.0 29 8.30616224648986 4.0 4.0 4.0 4.0 26.0 30 8.588533541341654 4.0 4.0 4.0 4.0 26.0 31 9.336973478939157 4.0 4.0 4.0 4.0 31.0 32 9.14625585023401 4.0 4.0 4.0 4.0 30.0 33 9.053822152886115 4.0 4.0 4.0 4.0 30.0 34 9.02808112324493 4.0 4.0 4.0 4.0 30.0 35 8.913026521060843 4.0 4.0 4.0 4.0 30.0 36 8.78588143525741 4.0 4.0 4.0 4.0 30.0 37 8.518330733229329 4.0 4.0 4.0 4.0 29.0 38 8.360374414976599 4.0 4.0 4.0 4.0 29.0 39 8.310452418096723 4.0 4.0 4.0 4.0 28.0 40 8.562792511700469 4.0 4.0 4.0 4.0 30.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 15.751074735449734 1 2 14.372233245149912 1 3 4.556492504409171 1 4 15.240625 1 5 19.973137125220454 1 6 -8.358190035273374 1 7 -9.255483906525573 1 8 -9.267019400352735 1 9 -9.183107363315697 1 10 -8.211739417989419 1 11 -8.200540123456792 1 12 -7.749564594356265 1 13 -7.782319223985889 1 14 -6.9044312169312185 1 15 -7.282357804232806 1 16 -4.505869708994707 1 17 -4.228725749559082 1 18 -4.4559082892416235 1 19 -4.4978835978835985 1 20 -4.642537477954145 1 21 -4.093187830687832 1 22 -4.286017416225748 1 23 -4.210041887125222 1 24 -3.952639991181659 1 25 -3.848247354497352 1 26 -4.190013227513228 1 27 -4.1657848324515 1 28 -4.295221560846564 1 29 -3.7619047619047628 1 30 -3.9878968253968266 1 31 -4.058476631393299 1 32 -4.059397045855377 1 33 -3.623748897707232 1 34 -3.584319885361552 1 35 -3.7860559964726646 1 36 -3.51178902116402 1 37 -3.6429618606701943 1 38 -3.4099757495590826 1 39 -3.3398754409171074 1 40 -3.4628692680776014 4 1 3.1510747354497326 4 2 -6.027766754850088 4 3 -4.24350749559083 4 4 -5.559375 4 5 -7.426862874779546 4 6 -3.1581900352733747 4 7 -7.255483906525573 4 8 -7.667019400352736 4 9 -5.783107363315697 4 10 -2.611739417989419 4 11 -6.200540123456792 4 12 -2.9495645943562643 4 13 -3.5823192239858894 4 14 -5.9044312169312185 4 15 -3.0823578042328066 4 16 -1.305869708994707 4 17 -1.028725749559082 4 18 -2.055908289241623 4 19 -2.2978835978835983 4 20 -3.4425374779541444 4 21 -0.6931878306878314 4 22 -1.6860174162257486 4 23 -2.0100418871252215 4 24 -1.3526399911816593 4 25 -2.2482473544973525 4 26 1.8099867724867718 4 27 -2.1657848324515 4 28 -1.2952215608465636 4 29 2.038095238095238 4 30 2.0121031746031734 4 31 -2.658476631393299 4 32 0.3406029541446234 4 33 -1.0237488977072324 4 34 -0.9843198853615522 4 35 1.4139440035273347 4 36 1.6882109788359791 4 37 1.557038139329805 4 38 -3.4099757495590826 4 39 -3.3398754409171074 4 40 -3.4628692680776014 5 1 -1.248925264550266 5 2 -6.377766754850088 5 3 -1.6935074955908291 5 4 -5.759375 5 5 -2.276862874779546 5 6 -1.108190035273374 5 7 -9.255483906525573 5 8 -9.267019400352735 5 9 -9.183107363315697 5 10 -8.211739417989419 5 11 -8.200540123456792 5 12 -7.749564594356265 5 13 -7.782319223985889 5 14 -6.9044312169312185 5 15 -7.282357804232806 5 16 -4.505869708994707 5 17 -4.228725749559082 5 18 -4.4559082892416235 5 19 -4.4978835978835985 5 20 -4.642537477954145 5 21 -4.093187830687832 5 22 -4.286017416225748 5 23 -4.210041887125222 5 24 -3.952639991181659 5 25 -3.848247354497352 5 26 -4.190013227513228 5 27 -4.1657848324515 5 28 -4.295221560846564 5 29 -3.7619047619047628 5 30 -3.9878968253968266 5 31 -4.058476631393299 5 32 -4.059397045855377 5 33 -3.623748897707232 5 34 -3.584319885361552 5 35 -3.7860559964726646 5 36 -3.51178902116402 5 37 -3.6429618606701943 5 38 -3.4099757495590826 5 39 -3.3398754409171074 5 40 -3.4628692680776014 6 1 15.417741402116398 6 2 21.03889991181658 6 3 15.55649250440917 6 4 16.407291666666666 6 5 18.13980379188712 6 6 17.808476631393294 6 7 12.57784942680776 6 8 19.5663139329806 6 9 19.3168926366843 6 10 6.788260582010581 6 11 10.63279320987654 6 12 19.417102072310403 6 13 16.05101410934744 6 14 12.76223544973545 6 15 18.884308862433862 6 16 15.994130291005293 6 17 20.437940917107586 6 18 14.377425044091709 6 19 10.835449735449734 6 20 18.52412918871252 6 21 11.906812169312168 6 22 18.880649250440918 6 23 17.456624779541446 6 24 13.047360008818341 6 25 16.151752645502647 6 26 16.309986772486774 6 27 19.8342151675485 6 28 18.371445105820104 6 29 12.57142857142857 6 30 16.845436507936505 6 31 19.27485670194003 6 32 22.10726962081129 6 33 15.2095844356261 6 34 20.415680114638448 6 35 20.713944003527335 6 36 23.654877645502648 6 37 17.357038139329806 6 38 20.423357583774248 6 39 20.993457892416224 6 40 22.87046406525573 7 1 8.751074735449734 7 2 7.483344356261023 7 3 7.667603615520282 7 4 -1.7593750000000004 7 5 8.195359347442675 7 6 7.752921075837737 7 7 3.9667383156966487 7 8 8.510758377425043 7 9 4.594670414462081 7 10 9.788260582010581 7 11 3.6883487654320977 7 12 10.139324294532624 7 13 4.106569664903001 7 14 6.762235449735449 7 15 4.050975529100528 7 16 4.160796957671959 7 17 5.215718694885362 7 18 8.321869488536155 7 19 9.835449735449734 7 20 9.579684744268077 7 21 10.906812169312168 7 22 5.158427028218696 7 23 5.567735890652557 7 24 7.93624889770723 7 25 8.818419312169315 7 26 9.809986772486772 7 27 9.72310405643739 7 28 11.704778439153436 7 29 7.904761904761903 7 30 9.012103174603173 7 31 11.83041225749559 7 32 13.385047398589066 7 33 11.154028880070546 7 34 11.971235670194005 7 35 13.436166225749556 7 36 11.48821097883598 7 37 14.134815917107584 7 38 7.367802028218696 7 39 7.549013447971782 7 40 9.981575176366842 9 1 11.751074735449734 9 2 9.372233245149912 9 3 9.55649250440917 9 4 -1.7593750000000004 9 5 3.973137125220454 9 6 14.641809964726626 9 7 19.744516093474427 9 8 -0.2670194003527353 9 9 16.8168926366843 9 10 13.788260582010581 9 11 1.7994598765432084 9 12 12.250435405643735 9 13 18.217680776014113 9 14 11.095568783068781 9 15 14.717642195767194 9 16 10.494130291005293 9 17 3.771274250440918 9 18 7.5440917107583765 9 19 4.5021164021164015 9 20 18.357462522045857 9 21 1.9068121693121682 9 22 17.713982583774253 9 23 12.789958112874778 9 24 16.04736000881834 9 25 5.151752645502648 9 26 10.809986772486772 9 27 7.8342151675485 9 28 14.704778439153436 9 29 12.238095238095237 9 30 14.012103174603173 9 31 22.941523368606703 9 32 13.940602954144623 9 33 11.376251102292768 9 34 4.415680114638448 9 35 15.213944003527335 9 36 18.48821097883598 9 37 16.357038139329806 9 38 20.59002425044092 9 39 25.66012455908289 9 40 22.5371307319224 10 1 1.1510747354497326 10 2 -4.427766754850087 10 3 -3.043507495590829 10 4 -2.1593750000000007 10 5 -5.226862874779546 10 6 -3.9581900352733737 10 7 -2.2554839065255727 10 8 -1.2670194003527353 10 9 -1.7831073633156969 10 10 -1.411739417989418 10 11 3.1994598765432087 10 12 4.050435405643736 10 13 0.017680776014112 10 14 2.895568783068782 10 15 4.717642195767194 10 16 2.694130291005292 10 17 0.7712742504409178 10 18 0.7440917107583758 10 19 -2.2978835978835983 10 20 2.3574625220458554 10 21 1.3068121693121686 10 22 2.5139825837742524 10 23 2.9899581128747776 10 24 3.047360008818341 10 25 4.7517526455026475 10 26 5.009986772486771 10 27 3.2342151675485002 10 28 5.704778439153436 10 29 2.038095238095238 10 30 3.612103174603173 10 31 6.341523368606701 10 32 7.940602954144623 10 33 5.376251102292768 10 34 8.615680114638447 10 35 8.413944003527334 10 36 2.88821097883598 10 37 2.757038139329806 10 38 2.5900242504409174 10 39 2.2601245590828922 10 40 2.137130731922398 11 1 -0.498925264550266 11 2 -5.127766754850088 11 3 0.5564925044091709 11 4 -1.2593750000000004 11 5 -1.026862874779546 11 6 1.641809964726626 11 7 3.2445160934744273 11 8 0.2329805996472647 11 9 -0.9331073633156972 11 10 4.788260582010581 11 11 1.0494598765432084 11 12 4.000435405643735 11 13 -0.03231922398588871 11 14 5.3455687830687815 11 15 2.467642195767194 11 16 -3.005869708994707 11 17 1.2712742504409178 11 18 -2.2059082892416235 11 19 1.0021164021164015 11 20 -2.6425374779541446 11 21 -3.3431878306878318 11 22 -1.0360174162257483 11 23 0.5399581128747784 11 24 1.047360008818341 11 25 0.1517526455026479 11 26 -0.9400132275132282 11 27 3.0842151675485 11 28 -2.0452215608465636 11 29 0.48809523809523725 11 30 3.2621031746031734 11 31 3.441523368606701 11 32 2.440602954144623 11 33 3.876251102292768 11 34 0.4156801146384481 11 35 0.21394400352733545 11 36 0.48821097883597986 11 37 -0.39296186067019434 11 38 3.5900242504409174 11 39 -3.3398754409171074 11 40 -3.4628692680776014 12 1 16.751074735449734 12 2 21.372233245149914 12 3 11.55649250440917 12 4 17.240625 12 5 12.973137125220454 12 6 20.641809964726626 12 7 20.744516093474427 12 8 15.732980599647265 12 9 15.816892636684303 12 10 20.78826058201058 12 11 14.799459876543208 12 12 23.250435405643735 12 13 23.217680776014113 12 14 16.09556878306878 12 15 17.717642195767194 12 16 4.494130291005293 12 17 14.771274250440918 12 18 12.544091710758376 12 19 24.5021164021164 12 20 -4.642537477954145 12 21 -4.093187830687832 12 22 -4.286017416225748 12 23 -4.210041887125222 12 24 -3.952639991181659 12 25 -3.848247354497352 12 26 -4.190013227513228 12 27 -4.1657848324515 12 28 -4.295221560846564 12 29 -3.7619047619047628 12 30 -3.9878968253968266 12 31 -4.058476631393299 12 32 -4.059397045855377 12 33 -3.623748897707232 12 34 -3.584319885361552 12 35 -3.7860559964726646 12 36 -3.51178902116402 12 37 -3.6429618606701943 12 38 -3.4099757495590826 12 39 -3.3398754409171074 12 40 -3.4628692680776014 13 1 8.501074735449734 13 2 2.6222332451499124 13 3 1.9314925044091709 13 4 -1.1343750000000004 13 5 2.473137125220454 13 6 11.016809964726626 13 7 11.619516093474427 13 8 11.857980599647265 13 9 9.941892636684303 13 10 7.538260582010581 13 11 13.174459876543208 13 12 11.875435405643735 13 13 8.592680776014111 13 14 9.845568783068781 13 15 5.092642195767194 13 16 7.494130291005293 13 17 5.896274250440918 13 18 5.4190917107583765 13 19 3.3771164021164015 13 20 4.107462522045855 13 21 7.531812169312168 13 22 5.213982583774252 13 23 6.789958112874778 13 24 1.422360008818341 13 25 5.901752645502648 13 26 4.434986772486772 13 27 6.3342151675485 13 28 10.829778439153436 13 29 4.863095238095237 13 30 8.387103174603173 13 31 10.1915233686067 13 32 10.440602954144623 13 33 7.876251102292768 13 34 9.665680114638448 13 35 11.588944003527335 13 36 10.86321097883598 13 37 9.232038139329806 13 38 10.340024250440917 13 39 13.785124559082892 13 40 11.5371307319224 14 1 -14.248925264550266 14 2 -7.627766754850088 14 3 -8.44350749559083 14 4 -6.759375 14 5 -9.026862874779546 14 6 -8.358190035273374 14 7 -9.255483906525573 14 8 -9.267019400352735 14 9 -9.183107363315697 14 10 -8.211739417989419 14 11 -8.200540123456792 14 12 -7.749564594356265 14 13 -7.782319223985889 14 14 -6.9044312169312185 14 15 -7.282357804232806 14 16 -4.505869708994707 14 17 -4.228725749559082 14 18 -4.4559082892416235 14 19 -4.4978835978835985 14 20 -4.642537477954145 14 21 -4.093187830687832 14 22 -4.286017416225748 14 23 -4.210041887125222 14 24 -3.952639991181659 14 25 -3.848247354497352 14 26 -4.190013227513228 14 27 -4.1657848324515 14 28 -4.295221560846564 14 29 -3.7619047619047628 14 30 -3.9878968253968266 14 31 -4.058476631393299 14 32 -4.059397045855377 14 33 -3.623748897707232 14 34 -3.584319885361552 14 35 -3.7860559964726646 14 36 -3.51178902116402 14 37 -3.6429618606701943 14 38 -3.4099757495590826 14 39 -3.3398754409171074 14 40 -3.4628692680776014 16 1 14.751074735449734 16 2 12.372233245149912 16 3 13.05649250440917 16 4 16.240625 16 5 17.473137125220454 16 6 0.641809964726626 16 7 10.744516093474427 16 8 21.732980599647263 16 9 19.3168926366843 16 10 13.788260582010581 16 11 12.299459876543208 16 12 -0.749564594356265 16 13 8.717680776014111 16 14 7.0955687830687815 16 15 17.217642195767194 16 16 10.994130291005293 16 17 0.7712742504409178 16 18 10.044091710758376 16 19 0.5021164021164015 16 20 8.357462522045855 16 21 9.406812169312168 16 22 3.2139825837742517 16 23 -1.2100418871252216 16 24 -0.452639991181659 16 25 9.651752645502647 16 26 9.309986772486772 16 27 4.8342151675485 16 28 9.704778439153436 16 29 -0.7619047619047628 16 30 5.0121031746031734 16 31 9.9415233686067 16 32 8.940602954144623 16 33 10.876251102292768 16 34 9.415680114638448 16 35 11.213944003527335 16 36 4.48821097883598 16 37 12.357038139329806 16 38 11.090024250440917 16 39 9.660124559082892 16 40 11.0371307319224 17 1 8.417741402116402 17 2 8.872233245149912 17 3 7.223159171075839 17 4 -1.2593750000000004 17 5 10.973137125220454 17 6 10.641809964726626 17 7 10.911182760141095 17 8 8.066313932980597 17 9 9.98355930335097 17 10 10.121593915343913 17 11 16.13279320987654 17 12 18.083768738977067 17 13 12.551014109347443 17 14 15.595568783068781 17 15 13.717642195767194 17 16 13.994130291005293 17 17 14.437940917107586 17 18 14.377425044091709 17 19 16.16878306878307 17 20 13.190795855379188 17 21 10.573478835978836 17 22 17.047315917107582 17 23 8.789958112874778 17 24 10.714026675485009 17 25 8.151752645502647 17 26 8.809986772486772 17 27 10.167548500881832 17 28 7.204778439153436 17 29 4.571428571428571 17 30 9.845436507936505 17 31 15.774856701940033 17 32 12.773936287477955 17 33 9.542917768959436 17 34 10.915680114638448 17 35 11.213944003527335 17 36 14.154877645502648 17 37 10.857038139329806 17 38 15.090024250440917 17 39 13.660124559082892 17 40 15.870464065255732 18 1 6.626074735449734 18 2 4.622233245149912 18 3 8.93149250440917 18 4 -1.7593750000000004 18 5 3.973137125220454 18 6 8.516809964726626 18 7 7.244516093474427 18 8 8.607980599647265 18 9 8.316892636684303 18 10 7.038260582010581 18 11 11.674459876543208 18 12 5.750435405643735 18 13 10.092680776014111 18 14 6.8455687830687815 18 15 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-0.9978835978835985 107 20 4.357462522045855 107 21 4.906812169312168 107 22 3.7139825837742517 107 23 3.2899581128747784 107 24 5.047360008818341 107 25 4.651752645502648 107 26 7.809986772486772 107 27 12.3342151675485 107 28 12.204778439153436 107 29 9.238095238095237 107 30 10.012103174603173 107 31 0.9415233686067008 107 32 0.440602954144623 107 33 5.376251102292768 107 34 9.415680114638448 107 35 3.7139440035273354 107 36 2.98821097883598 107 37 6.357038139329806 107 38 9.590024250440917 107 39 -3.3398754409171074 107 40 -3.4628692680776014 108 1 13.084408068783066 108 2 11.372233245149912 108 3 7.556492504409171 108 4 9.907291666666667 108 5 11.306470458553786 108 6 -7.024856701940041 108 7 4.077849426807759 108 8 3.3996472663139325 108 9 7.483559303350971 108 10 9.121593915343913 108 11 3.7994598765432084 108 12 -1.416231261022931 108 13 4.217680776014111 108 14 1.0955687830687815 108 15 5.384308862433862 108 16 8.16079695767196 108 17 9.771274250440918 108 18 9.210758377425044 108 19 9.502116402116402 108 20 6.690795855379189 108 21 11.573478835978836 108 22 11.713982583774252 108 23 10.789958112874778 108 24 9.047360008818341 108 25 7.818419312169314 108 26 3.1433201058201057 108 27 4.167548500881834 108 28 3.0381117724867703 108 29 3.571428571428571 108 30 4.0121031746031734 108 31 4.274856701940035 108 32 3.940602954144623 108 33 4.376251102292768 108 34 4.749013447971782 108 35 3.5472773368606694 108 36 7.821544312169314 108 37 7.69037147266314 108 38 7.923357583774251 108 39 7.326791225749559 108 40 7.8704640652557325 110 1 -9.848925264550266 110 2 -6.827766754850088 110 3 -6.643507495590829 110 4 -5.759375 110 5 -5.026862874779546 110 6 -5.358190035273374 110 7 -7.055483906525573 110 8 -8.867019400352735 110 9 -8.183107363315697 110 10 -4.611739417989419 110 11 -6.800540123456791 110 12 -3.949564594356265 110 13 -6.5823192239858885 110 14 -5.704431216931218 110 15 -5.282357804232806 110 16 -1.505869708994707 110 17 -1.028725749559082 110 18 -1.8559082892416239 110 19 -1.8978835978835988 110 20 -3.6425374779541446 110 21 -0.8931878306878316 110 22 0.5139825837742524 110 23 0.7899581128747784 110 24 -0.15263999118165916 110 25 1.5517526455026482 110 26 0.009986772486771045 110 27 2.2342151675485002 110 28 0.9047784391534357 110 29 2.6380952380952376 110 30 1.2121031746031727 110 31 2.1415233686067 110 32 0.7406029541446237 110 33 2.576251102292767 110 34 1.6156801146384474 110 35 1.4139440035273347 110 36 -3.51178902116402 110 37 -3.6429618606701943 110 38 -3.4099757495590826 110 39 -3.3398754409171074 110 40 -3.4628692680776014 111 1 -0.748925264550266 111 2 -0.1277667548500876 111 3 0.8064925044091709 111 4 0.49062499999999964 111 5 -3.526862874779546 111 6 -0.858190035273374 111 7 -0.25548390652557273 111 8 -2.2670194003527353 111 9 0.3168926366843028 111 10 2.0382605820105812 111 11 -1.9505401234567916 111 12 0.500435405643735 111 13 -2.5323192239858887 111 14 -3.9044312169312185 111 15 3.467642195767194 111 16 2.494130291005293 111 17 2.521274250440918 111 18 3.0440917107583765 111 19 -0.4978835978835985 111 20 1.1074625220458554 111 21 4.656812169312168 111 22 7.213982583774252 111 23 8.039958112874778 111 24 6.797360008818341 111 25 8.401752645502647 111 26 7.059986772486772 111 27 4.5842151675485 111 28 6.704778439153436 111 29 4.738095238095237 111 30 7.0121031746031734 111 31 6.941523368606701 111 32 8.190602954144623 111 33 6.376251102292768 111 34 6.415680114638448 111 35 8.463944003527335 111 36 3.98821097883598 111 37 11.107038139329806 111 38 2.0900242504409174 111 39 4.660124559082893 111 40 6.537130731922399 112 1 0.751074735449734 112 2 -4.627766754850088 112 3 -3.193507495590829 112 4 -4.259375 112 5 -6.276862874779546 112 6 -1.858190035273374 112 7 -9.255483906525573 112 8 -9.267019400352735 112 9 -9.183107363315697 112 10 -8.211739417989419 112 11 -8.200540123456792 112 12 -7.749564594356265 112 13 -7.782319223985889 112 14 -6.9044312169312185 112 15 -7.282357804232806 112 16 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2.583768738977069 117 13 2.551014109347445 117 14 1.7622354497354475 117 15 3.050975529100528 117 16 -4.505869708994707 117 17 -4.228725749559082 117 18 -4.4559082892416235 117 19 -4.4978835978835985 117 20 -4.642537477954145 117 21 -4.093187830687832 117 22 -4.286017416225748 117 23 -4.210041887125222 117 24 -3.952639991181659 117 25 -3.848247354497352 117 26 -4.190013227513228 117 27 -4.1657848324515 117 28 -4.295221560846564 117 29 -3.7619047619047628 117 30 -3.9878968253968266 117 31 -4.058476631393299 117 32 -4.059397045855377 117 33 -3.623748897707232 117 34 -3.584319885361552 117 35 -3.7860559964726646 117 36 -3.51178902116402 117 37 -3.6429618606701943 117 38 -3.4099757495590826 117 39 -3.3398754409171074 117 40 -3.4628692680776014 119 1 -14.248925264550266 119 2 -7.627766754850088 119 3 -8.44350749559083 119 4 -6.759375 119 5 -9.026862874779546 119 6 -8.358190035273374 119 7 -9.255483906525573 119 8 -9.267019400352735 119 9 -9.183107363315697 119 10 -8.211739417989419 119 11 -8.200540123456792 119 12 -7.749564594356265 119 13 -7.782319223985889 119 14 -6.9044312169312185 119 15 -7.282357804232806 119 16 -4.505869708994707 119 17 -4.228725749559082 119 18 -4.4559082892416235 119 19 -4.4978835978835985 119 20 -4.642537477954145 119 21 -4.093187830687832 119 22 -4.286017416225748 119 23 -4.210041887125222 119 24 -3.952639991181659 119 25 -3.848247354497352 119 26 -4.190013227513228 119 27 -4.1657848324515 119 28 -4.295221560846564 119 29 -3.7619047619047628 119 30 -3.9878968253968266 119 31 -4.058476631393299 119 32 -4.059397045855377 119 33 -3.623748897707232 119 34 -3.584319885361552 119 35 -3.7860559964726646 119 36 -3.51178902116402 119 37 -3.6429618606701943 119 38 -3.4099757495590826 119 39 -3.3398754409171074 119 40 -3.4628692680776014 120 1 -14.248925264550266 120 2 -7.627766754850088 120 3 -8.44350749559083 120 4 -6.759375 120 5 -9.026862874779546 120 6 -8.358190035273374 120 7 -9.255483906525573 120 8 -9.267019400352735 120 9 -9.183107363315697 120 10 -8.211739417989419 120 11 -8.200540123456792 120 12 -7.749564594356265 120 13 -7.782319223985889 120 14 -6.9044312169312185 120 15 -7.282357804232806 120 16 -4.505869708994707 120 17 -4.228725749559082 120 18 -4.4559082892416235 120 19 -4.4978835978835985 120 20 -4.642537477954145 120 21 -4.093187830687832 120 22 -4.286017416225748 120 23 -4.210041887125222 120 24 -3.952639991181659 120 25 -3.848247354497352 120 26 -4.190013227513228 120 27 -4.1657848324515 120 28 -4.295221560846564 120 29 -3.7619047619047628 120 30 -3.9878968253968266 120 31 -4.058476631393299 120 32 -4.059397045855377 120 33 -3.623748897707232 120 34 -3.584319885361552 120 35 -3.7860559964726646 120 36 -3.51178902116402 120 37 -3.6429618606701943 120 38 -3.4099757495590826 120 39 -3.3398754409171074 120 40 -3.4628692680776014 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 1388.0 5 28.0 6 48.0 7 60.0 8 50.0 9 72.0 10 83.0 11 64.0 12 46.0 13 36.0 14 42.0 15 25.0 16 28.0 17 10.0 18 16.0 19 23.0 20 41.0 21 61.0 22 89.0 23 85.0 24 78.0 25 62.0 26 54.0 27 36.0 28 20.0 29 7.0 30 7.0 31 4.0 32 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 99.21905505661852 0.27333073018352205 0.27333073018352205 0.23428348301444746 8 97.14955095665756 1.3666536509176102 0.6638032018742679 0.8199921905505663 9 95.34974599452912 1.4458772958186792 2.0711215318483784 1.1332551778038296 10 34.21771361685525 24.65860319937573 29.41865001950839 11.705033164260632 11 21.21684867394696 27.223088923556944 16.926677067082682 34.63338533541342 12 27.262090483619346 39.31357254290172 17.901716068642745 15.522620904836193 13 20.04680187207488 26.365054602184085 18.29173166926677 35.29641185647426 14 16.660163870464302 49.43425673039407 18.962153726102223 14.943425673039407 15 44.61778471138845 25.234009360374415 19.85179407176287 10.29641185647426 16 20.826833073322934 46.64586583463339 22.46489859594384 10.062402496099844 17 22.620904836193446 23.16692667706708 42.1996879875195 12.012480499219969 18 20.982839313572544 25.78003120124805 22.03588143525741 31.201248049921997 19 20.405774483027702 24.61958642216153 22.00546234880999 32.96917674600078 20 20.16380655226209 27.418096723868956 40.6396255850234 11.778471138845553 21 19.38377535101404 24.336973478939157 23.673946957878314 32.605304212168484 22 15.210608424336975 23.08892355694228 41.69266770670827 20.00780031201248 23 14.709325009754195 29.028482247366366 39.75809598127194 16.504096761607492 24 16.69266770670827 30.81123244929797 20.74882995319813 31.747269890795632 25 17.4609375 26.09375 37.8125 18.6328125 26 39.781164517389605 27.00273544353263 18.79640484564283 14.419695193434936 27 17.665491578535057 45.24089306698003 18.762240501370936 18.33137485311398 28 25.927372120265524 42.21007418976962 18.781725888324875 13.080827801639986 29 30.778255768478687 35.510363707469686 21.392256550645286 12.319123973406336 30 28.042121684867393 27.418096723868956 28.31513260530421 16.22464898595944 31 28.939157566302654 37.246489859594384 20.00780031201248 13.806552262090483 32 27.699530516431924 35.406885758998435 22.535211267605636 14.358372456964005 33 27.84711388455538 37.01248049921997 20.865834633385337 14.274570982839313 34 18.213728549141965 25.975039001560063 44.383775351014044 11.42745709828393 35 15.678627145085802 30.46021840873635 42.394695787831516 11.466458658346333 36 26.309616888193904 28.655199374511337 29.163408913213445 15.871774824081314 37 28.23712948517941 27.14508580343214 32.293291731669264 12.324492979719189 38 28.549141965678626 28.666146645865837 29.017160686427456 13.76755070202808 39 25.156006240249614 29.758190327613104 20.514820592823714 24.570982839313572 40 26.98907956318253 26.482059282371296 30.148205928237132 16.380655226209047 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 1.0 10 1.0 11 2.5 12 4.0 13 4.0 14 19.5 15 35.0 16 42.0 17 49.0 18 49.0 19 44.0 20 39.0 21 32.5 22 26.0 23 26.0 24 31.0 25 36.0 26 32.0 27 28.0 28 28.0 29 34.5 30 41.0 31 46.0 32 51.0 33 51.0 34 47.0 35 43.0 36 66.5 37 90.0 38 90.0 39 103.0 40 116.0 41 154.5 42 193.0 43 193.0 44 220.0 45 247.0 46 221.5 47 196.0 48 196.0 49 199.0 50 202.0 51 234.5 52 267.0 53 267.0 54 314.0 55 361.0 56 281.5 57 202.0 58 202.0 59 181.5 60 161.0 61 131.5 62 102.0 63 102.0 64 72.0 65 42.0 66 31.5 67 21.0 68 21.0 69 12.5 70 4.0 71 3.0 72 2.0 73 2.0 74 2.5 75 3.0 76 2.0 77 1.0 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.1170046801872075 8 0.1170046801872075 9 0.19500780031201248 10 0.0390015600624025 11 0.0 12 0.0 13 0.0 14 0.0390015600624025 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0390015600624025 20 0.0 21 0.0 22 0.0 23 0.0390015600624025 24 0.0 25 0.15600624024961 26 0.19500780031201248 27 0.4290171606864275 28 0.1170046801872075 29 0.27301092043681746 30 0.0 31 0.0 32 0.31201248049922 33 0.0 34 0.0 35 0.0 36 0.234009360374415 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 2564.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.78315132605304 #Duplication Level Percentage of deduplicated Percentage of total 1 94.15841584158416 74.18096723868955 2 3.2673267326732676 5.14820592823713 3 0.8415841584158417 1.989079563182527 4 0.594059405940594 1.87207488299532 5 0.24752475247524752 0.9750390015600624 6 0.1485148514851485 0.702028081123245 7 0.0 0.0 8 0.19801980198019803 1.24804992199688 9 0.0 0.0 >10 0.4455445544554455 5.889235569422777 >50 0.04950495049504951 3.5491419656786274 >100 0.04950495049504951 4.446177847113884 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGATCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 114 4.446177847113884 No Hit GAGATCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 91 3.5491419656786274 No Hit GAGATCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 25 0.9750390015600624 No Hit GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 22 0.858034321372855 No Hit GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21 0.8190327613104524 No Hit GAGATCGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG 18 0.702028081123245 No Hit GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 14 0.5460218408736349 No Hit GAGATCGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG 14 0.5460218408736349 No Hit GAGATCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.5070202808112325 No Hit GAGATCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 13 0.5070202808112325 No Hit GAGATCGGGGCTGAGATCGGGTCACAGATCCTCTTCTGAA 11 0.4290171606864275 No Hit GAGATCGGGGTGCAGTGCTGAGATCGGGTCACAGATCCTC 8 0.31201248049922 No Hit GAGATCGGGGCTGAGATCGGGTCACAGATCCTCTTCTGAG 8 0.31201248049922 No Hit GAGATCGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT 8 0.31201248049922 No Hit GAGATCGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT 8 0.31201248049922 No Hit GAGATCGGGTCACAGATCCTCTTCTGAGATGAGGATCGGA 6 0.234009360374415 No Hit GAGATCGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT 6 0.234009360374415 No Hit GAGATCGGGTCAAAGATCCTATTCTGAAGGAGATTGGGCG 6 0.234009360374415 No Hit GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT 5 0.19500780031201248 No Hit GAGATCGGGCGGATTGAACAAAAACTCATCTCAGAAGAGG 5 0.19500780031201248 No Hit GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 5 0.19500780031201248 No Hit GAGATCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.19500780031201248 No Hit GAGATCGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.19500780031201248 No Hit GAGATCGGGCCACAGATCCTCTTCTGAAGGAGATTGGGCG 4 0.15600624024961 No Hit GAGATCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT 4 0.15600624024961 No Hit GAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGATCG 4 0.15600624024961 Illumina Single End Adapter 2 (100% over 34bp) GAGATCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT 4 0.15600624024961 No Hit GAGATCGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGG 4 0.15600624024961 No Hit GAGATCGGGTCACAGATCCTCTTAAGGAGATTGGGCGGAT 4 0.15600624024961 No Hit GAGATCGGGAGCCTACACCTGTTCTAAGCCAATCAGCAAA 4 0.15600624024961 No Hit GAGATCGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4 0.15600624024961 No Hit GAGATCGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA 4 0.15600624024961 No Hit GAGATCGGGTCACAGATAATCTTCTGAAGGAGATTGGGCG 4 0.15600624024961 No Hit GAGATCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4 0.15600624024961 No Hit GAGATCGGGGAGTGCAGTGCTGAGATCGGGGAAAAAAAAA 4 0.15600624024961 No Hit GAGATCGGGGCTTCTTGTATGATCATGGGCATCTCTTTCT 3 0.1170046801872075 No Hit GAGATCGGGTCCTCTTCTGAAGGAGATTGGGCGGATTGAA 3 0.1170046801872075 No Hit GAGATCGGGCTGAGATCGGGTCACAGATCCTCTTCTGAAG 3 0.1170046801872075 No Hit GAGATCGGGTCACAGATCATCTTCTGAAGGAGATTGGGCG 3 0.1170046801872075 No Hit GAGATCGGGTCAAAGATACTCTTCTGAGATGAGTTTTTGT 3 0.1170046801872075 No Hit GAGATCGGGGAGGAGAAGCCCGGTTTACGCTGATGACCGA 3 0.1170046801872075 No Hit GAGATCGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG 3 0.1170046801872075 No Hit GAGATCGGGCGGATTGAACAACAACTCATCTCAGAAGAGG 3 0.1170046801872075 No Hit GAGATCGGGTCACAGATTCTCTTCTGAAGGAGATTGGGCG 3 0.1170046801872075 No Hit GAGATCGGCTCACAGATCCTCTTCTGAAGGAGATTGGGCG 3 0.1170046801872075 No Hit GAGATCGGGGCTGGGCAGTGGTGGCACATGCCTTTAATCC 3 0.1170046801872075 No Hit GAGATCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGC 3 0.1170046801872075 No Hit GAGATCGGATCACAGATCCTCTTCTGAGATGAGTTTTTGT 3 0.1170046801872075 No Hit GAGATCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT 3 0.1170046801872075 No Hit GAGATCGAGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 3 0.1170046801872075 No Hit GAGATCGGGCGGATTGAAAAAAAACTCATCTCAGAAGAGG 3 0.1170046801872075 No Hit GAGATCGGCAGTGATTGATGTGTTTGCTATGCCACAGTCA 3 0.1170046801872075 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.35101404056162244 0.0 0.0 0.0 0.0 3 0.35101404056162244 0.0 0.0 0.0 0.0 4 0.35101404056162244 0.0 0.0 0.0 0.0 5 0.35101404056162244 0.0 0.0 0.0 0.0 6 0.35101404056162244 0.0 0.0 0.0 0.0 7 0.35101404056162244 0.0 0.0 0.0 0.0 8 0.35101404056162244 0.0 0.0 0.0 0.0 9 0.35101404056162244 0.0 0.0 0.0 0.0 10 0.35101404056162244 0.0 0.0 0.0 0.0 11 0.35101404056162244 0.0 0.0 0.0 0.0 12 0.35101404056162244 0.0 0.0 0.0 0.0 13 0.35101404056162244 0.0 0.0 0.0 0.0 14 0.35101404056162244 0.0 0.0 0.0 0.0 15 0.35101404056162244 0.0 0.0 0.0 0.0 16 0.35101404056162244 0.0 0.0 0.0 0.0 17 0.35101404056162244 0.0 0.0 0.0 0.0 18 0.35101404056162244 0.0 0.0 0.0 0.0 19 0.35101404056162244 0.0 0.0 0.0 0.0 20 0.35101404056162244 0.0 0.0 0.0 0.0 21 0.35101404056162244 0.0 0.0 0.0 0.0 22 0.39001560062402496 0.0 0.0 0.0 0.0 23 0.39001560062402496 0.0 0.0 0.0 0.0 24 0.39001560062402496 0.0 0.0 0.0 0.0 25 0.39001560062402496 0.0 0.0 0.0 0.0 26 0.39001560062402496 0.0 0.0 0.0 0.0 27 0.39001560062402496 0.0 0.0 0.0 0.0 28 0.39001560062402496 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCTGAA 25 1.5435615E-4 33.901962 22 ATCGGGC 30 1.0057232E-5 33.901962 4 ATCGGGA 70 0.0 33.901962 4 TCGGGGT 20 0.002334186 33.901962 5 TCGGGGG 40 4.0665327E-8 33.901962 5 TCGGGGC 20 0.002334186 33.901962 5 TCGGGAG 30 1.0057232E-5 33.901962 5 TCGGGAA 30 1.0057232E-5 33.901962 5 GAGATCG 265 0.0 32.622643 1 ATCGGGG 90 0.0 32.01852 4 AGATCGG 260 0.0 31.94608 2 GATCGGG 245 0.0 31.826332 3 TCACAGA 45 1.1483644E-7 30.135077 10 CACAGAT 45 1.1483644E-7 30.135077 11 CTTCTGA 45 1.1483644E-7 30.135077 21 GTCACAG 40 1.8360224E-6 29.664215 9 ACAGATC 40 1.8360224E-6 29.664215 12 CTCTTCT 40 1.8360224E-6 29.664215 19 GGTCACA 40 1.8360224E-6 29.664215 8 CAGATCC 40 1.8360224E-6 29.664215 13 >>END_MODULE