SRR1525814_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1525814_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12898
Sequences flagged as poor quality	0
Sequence length	56
%GC	45

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAGTACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	789	6.117227477128237	No Hit
AAGTACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	653	5.062800434175841	No Hit
AAGTACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	231	1.790975345014731	No Hit
AAGTACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	186	1.4420840440378353	No Hit
AAGTACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	118	0.9148705225616375	No Hit
AAGTACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	81	0.6280043417584121	No Hit
AAGTACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	57	0.44192898123740115	No Hit
AAGTACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	56	0.43417584121569236	No Hit
AAGTACGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	54	0.4186695611722748	No Hit
AAGTACGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGAGCTCGTATGCCGT	51	0.3954101411071484	Illumina Single End Adapter 2 (95% over 24bp)
AAGTACGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC	43	0.33338502093347805	No Hit
AAGTACGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGATATCGTATGCCGT	39	0.3023724608466429	No Hit
AAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32	0.24810048069468132	No Hit
AAGTACGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATC	31	0.24034734067297256	No Hit
AAGTACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	29	0.22484106062955495	No Hit
AAGTACGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	29	0.22484106062955495	No Hit
AAGTACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	27	0.2093347805861374	No Hit
AAGTACGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGATCTCGTATGCCGT	27	0.2093347805861374	Illumina Single End Adapter 1 (95% over 23bp)
AAGTACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAAAAA	26	0.20158164056442862	No Hit
AAGTACGGGTCCCCCCTCCTCTTCTGAGATGAGTTTTTGTTCCCTCCGCCCAATCT	24	0.186075360521011	No Hit
AAGTACGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGAGCTCGTATGCCGT	23	0.17832222049930221	Illumina Single End Adapter 1 (95% over 23bp)
AAGTACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA	23	0.17832222049930221	No Hit
AAGTACGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCA	22	0.17056908047759342	No Hit
AAGTACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTA	22	0.17056908047759342	No Hit
AAGTACGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGATATCGTATGCCGT	19	0.14730966041246707	No Hit
AAGTACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAGAT	18	0.13955652039075825	No Hit
AAGTACGGCTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	18	0.13955652039075825	No Hit
AAGTACGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACT	17	0.13180338036904948	No Hit
AAGTACGGGTCACACATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	16	0.12405024034734066	No Hit
AAGTACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTCTTTTTTTTTTTTTTT	15	0.11629710032563188	No Hit
AAGTACGGGTCACATATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	15	0.11629710032563188	No Hit
AAGTACGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	15	0.11629710032563188	No Hit
AAGTACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15	0.11629710032563188	No Hit
AAGTACGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGCTTGAACAAAAACTCA	14	0.10854396030392309	No Hit
AAGTACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14	0.10854396030392309	No Hit
AAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14	0.10854396030392309	No Hit
AAGTACGGGAGTGCAGTGCTAAGTACGGGTCACAGATCCTCTTCTGAGATGAGTTT	14	0.10854396030392309	No Hit
AAGTACGGGTCCCCCCTCCTCTTCTGAAGGAGATTGGGCGGATCGACACAAAACTC	14	0.10854396030392309	No Hit
AAGTACGGGTCACAGATCCTCTTCTGAGATGAGGATCGGCAGAGCTCGTATGCCGT	14	0.10854396030392309	Illumina Single End Adapter 1 (95% over 23bp)
AAGTACGGGGTGCAGTGCTAAGTACGGGTCACAGATCCTCTTCTGAAGGAGATTGG	13	0.10079082028221431	No Hit
AAGTACGGGGTGCAGTGCTAAGTACGGGTCACAGATCCTCTTCTGAGATGAGTTTT	13	0.10079082028221431	No Hit
AAGTACGGGTCACAGATCCTCTTCTGAGATGAGGATCGGACGATCTCGTATGCCGT	13	0.10079082028221431	No Hit
AAGTACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCTAATCCGCCCAATCT	13	0.10079082028221431	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTGAA	20	3.7374996E-4	50.761906	41
GATTGAC	30	8.7123226E-7	50.761906	41
AACCAAC	15	0.007761826	50.761906	41
GGCGCAT	20	3.8118608E-4	50.561268	37
GCGCATT	20	3.8118608E-4	50.561268	38
CGCATTG	20	3.8118608E-4	50.561268	39
GCATTGA	20	3.8118608E-4	50.561268	40
TTGTCCA	25	1.845647E-5	50.561264	37
AACAACA	15	0.007885037	50.561264	38
AAACAAC	15	0.007885037	50.561264	37
TGTCCAA	25	1.845647E-5	50.561264	38
CGGACGA	15	0.007885037	50.561264	37
TGGGCGC	20	3.8873928E-4	50.36221	35
TGGGCGA	20	3.8873928E-4	50.36221	35
GGGCGCA	20	3.8873928E-4	50.36221	36
GGGCGAA	20	3.8873928E-4	50.36221	36
CCAACAC	15	0.008009697	50.362206	43
ACCAACA	15	0.008009697	50.362206	42
CAACACA	15	0.008009697	50.362206	44
TCGGACG	15	0.008009697	50.362206	36

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content