FastQCFastQC Report
Fri 17 Jun 2016
SRR1525813_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525813_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4784
Sequences flagged as poor quality0
Sequence length40
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT110.229933110367893No Hit
AAGTACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT100.20903010033444816No Hit
AAGTACGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGC100.20903010033444816No Hit
AAGTACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT100.20903010033444816No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC90.18812709030100336No Hit
AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG80.16722408026755853No Hit
AAGTACGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT70.1463210702341137No Hit
AAGTACGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT60.1254180602006689No Hit
AAGTACGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.1254180602006689No Hit
AAGTACGGGCAGAGTCGATTTTTTTTTTTTTTTTTTTTTT50.10451505016722408No Hit
AAGTACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT50.10451505016722408No Hit
AAGTACGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC50.10451505016722408No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGCC251.7411725E-434.0000046
CGGGGAT251.7411725E-434.0000046
ACGGGTA251.7411725E-434.0000045
ACGGGGT251.7411725E-434.0000045
ACGGGAA502.5283953E-1034.0000045
CGGGGGG301.1976439E-534.06
CGGGGGC200.002519911434.06
CGGGGAC200.002519911434.06
TACGGGT551.6370905E-1134.04
TACGGGC551.6370905E-1134.04
CGGGATT200.002519911434.06
CGGGAAG301.1976439E-534.06
ACGGGGG700.034.05
ACGGGGA650.034.05
ACGGGAT405.574111E-834.05
ACGGGAG301.1976439E-534.05
AAGTACG4800.033.6458321
AGTACGG4750.033.6421052
GTACGGG4550.033.6263733
TACGGGG2000.033.154
Produced by FastQC (version 0.11.5)