##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525813_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4784
Sequences flagged as poor quality	0
Sequence length	40
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.219690635451506	28.0	21.0	33.0	4.0	35.0
2	21.257316053511705	23.0	14.0	29.0	4.0	33.0
3	21.88440635451505	24.0	16.0	30.0	4.0	35.0
4	24.17056856187291	28.0	19.0	33.0	4.0	36.0
5	21.687709030100333	24.0	13.0	30.0	4.0	35.0
6	16.011496655518396	15.0	11.0	20.0	4.0	33.0
7	20.98076923076923	24.0	11.0	30.0	4.0	34.0
8	22.339046822742475	26.0	11.0	31.0	4.0	35.0
9	20.98035117056856	24.0	10.0	30.0	4.0	34.0
10	18.56333612040134	19.0	7.0	28.0	4.0	33.0
11	20.16785117056856	22.0	4.0	31.0	4.0	35.0
12	19.67997491638796	22.0	4.0	30.0	4.0	35.0
13	20.05413879598662	22.0	4.0	31.0	4.0	35.0
14	20.54891304347826	24.0	4.0	31.0	4.0	35.0
15	19.150501672240804	20.0	4.0	31.0	4.0	35.0
16	19.15551839464883	20.0	4.0	31.0	4.0	35.0
17	19.441680602006688	22.0	4.0	32.0	4.0	35.0
18	19.48913043478261	22.0	4.0	32.0	4.0	35.0
19	17.57086120401338	17.0	4.0	30.0	4.0	35.0
20	18.839673913043477	20.0	4.0	31.0	4.0	35.0
21	16.93896321070234	15.0	4.0	30.0	4.0	35.0
22	16.871864548494983	14.0	4.0	30.0	4.0	35.0
23	16.916596989966557	15.0	4.0	30.0	4.0	35.0
24	16.08465719063545	12.0	4.0	29.0	4.0	34.0
25	15.585702341137123	8.0	4.0	29.0	4.0	34.0
26	13.553511705685619	4.0	4.0	26.0	4.0	33.0
27	13.626254180602007	4.0	4.0	26.0	4.0	33.0
28	13.107232441471572	4.0	4.0	25.0	4.0	33.0
29	12.981814381270903	4.0	4.0	25.0	4.0	33.0
30	12.270903010033445	4.0	4.0	23.0	4.0	33.0
31	12.011496655518394	4.0	4.0	23.0	4.0	33.0
32	11.488921404682275	4.0	4.0	20.0	4.0	32.0
33	10.973871237458194	4.0	4.0	18.0	4.0	31.0
34	10.423913043478262	4.0	4.0	15.0	4.0	31.0
35	10.05121237458194	4.0	4.0	15.0	4.0	30.0
36	9.854724080267559	4.0	4.0	13.0	4.0	31.0
37	9.965301003344482	4.0	4.0	10.0	4.0	32.0
38	9.512332775919733	4.0	4.0	4.0	4.0	31.0
39	9.378971571906355	4.0	4.0	4.0	4.0	31.0
40	9.132107023411372	4.0	4.0	4.0	4.0	31.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	3.3248226613322664
1	2	4.022138742276809
1	3	-6.3823253044141435
1	4	2.7199734805677203
1	5	6.379114019900339
1	6	6.968557233083045
1	7	9.94657690915194
1	8	4.036857068834859
1	9	3.126390078175792
1	10	6.6935623583432715
1	11	4.4189149419341405
1	12	6.185483540735635
1	13	-3.4286647526143277
1	14	3.72859568236219
1	15	-0.05520830543338917
1	16	0.14980442559974705
1	17	2.3589249269471395
1	18	-1.0266222123214916
1	19	-0.9728792349440578
1	20	1.2709625775051983
1	21	6.753988413547235
1	22	4.405173441337917
1	23	4.170581347757228
1	24	-0.647176716174318
1	25	2.9259402895357294
1	26	2.3632403927271923
1	27	2.987609685898997
1	28	3.561315948290737
1	29	4.506756267275476
1	30	3.658497516555741
1	31	3.843630289278547
1	32	2.987770133028233
1	33	0.9940126333583716
1	34	-0.8295870962437597
1	35	-1.743696917661504
1	36	-0.6752019938844693
1	37	-1.3605137726086136
1	38	-2.865240323568657
1	39	0.5797865016802586
1	40	-1.3241794886452762
5	1	0.883646190744031
5	2	-11.757273022429075
5	3	2.0735570485270323
5	4	-2.7653206370793377
5	5	3.820290490488574
5	6	-3.810854531622839
5	7	-7.906364267318647
5	8	-9.536672342929847
5	9	-8.123609921824208
5	10	-3.5858494063626125
5	11	4.5365620007576695
5	12	-2.520398812205542
5	13	2.380158776797437
5	14	-3.389051376461339
5	15	7.253615223978375
5	16	2.2086279550115115
5	17	-2.317545661288154
5	18	6.002789552384392
5	19	-3.369938058473469
5	20	-2.1555080107300952
5	21	-8.260717468805707
5	22	-0.9624736174856121
5	23	-9.31471276988983
5	24	-4.132470833821376
5	25	-3.6770008869348576
5	26	-4.842641960213983
5	27	-1.6153314905715916
5	28	-5.718095816415145
5	29	-2.6255966739009953
5	30	-2.297384836385435
5	31	-1.6857814754273353
5	32	-0.8063475140305911
5	33	1.8616596921819006
5	34	-2.476645919773171
5	35	0.03571484704437822
5	36	2.4571509472920017
5	37	-5.06639612554979
5	38	-4.688769735333363
5	39	-4.008448792437388
5	40	-3.677120665115864
6	1	1.383646190744031
6	2	2.492726977570925
6	3	1.8235570485270323
6	4	1.4846793629206623
6	5	-4.179709509511426
6	6	0.43914546837716095
6	7	-0.40636426731864717
6	8	-12.286672342929847
6	9	-0.37360992182420816
6	10	11.164150593637387
6	11	6.0365620007576695
6	12	-0.5203988122055421
6	13	2.630158776797437
6	14	12.110948623538661
6	15	13.503615223978375
6	16	0.7086279550115115
6	17	1.682454338711846
6	18	2.0027895523843924
6	19	-7.119938058473469
6	20	0.09449198926990476
6	21	2.489282531194293
6	22	-0.2124736174856121
6	23	2.43528723011017
6	24	2.617529166178624
6	25	5.572999113065142
6	26	8.657358039786017
6	27	8.634668509428408
6	28	9.031904183584855
6	29	9.624403326099005
6	30	7.452615163614565
6	31	8.814218524572665
6	32	8.193652485969409
6	33	1.1116596921819006
6	34	10.023354080226829
6	35	9.285714847044378
6	36	-4.792849052707998
6	37	-5.06639612554979
6	38	-4.688769735333363
6	39	-4.008448792437388
6	40	-3.677120665115864
7	1	10.383646190744031
7	2	12.77844126328521
7	3	12.609271334241317
7	4	10.198965077206378
7	5	4.606004776202859
7	6	12.296288325520019
7	7	4.736492875538495
7	8	2.2133276570701526
7	9	9.554961506747219
7	10	8.164150593637387
7	11	4.5365620007576695
7	12	10.336744044937316
7	13	6.4873016339402945
7	14	12.610948623538661
7	15	10.146472366835518
7	16	4.637199383582939
7	17	9.753882910140419
7	18	6.074218123812965
7	19	1.380061941526531
7	20	7.80877770355562
7	21	6.846425388337151
7	22	7.1446692396572455
7	23	5.649572944395885
7	24	7.831814880464339
7	25	8.144427684493715
7	26	6.371643754071732
7	27	12.206097080856981
7	28	11.603332755013428
7	29	7.481546183241862
7	30	-1.618813407814006
7	31	0.7427899531440936
7	32	-2.0206332283163047
7	33	0.5402311207533295
7	34	1.3804969373696867
7	35	0.5000005613300917
7	36	-0.22142048127942715
7	37	2.9336038744502098
7	38	-0.5459125924762205
7	39	-4.008448792437388
7	40	-3.677120665115864
9	1	-1.616353809255969
9	2	-3.507273022429075
9	3	0.32355704852703226
9	4	6.484679362920662
9	5	-0.6797095095114258
9	6	-1.560854531622839
9	7	0.5936357326813528
9	8	-0.7866723429298474
9	9	8.126390078175792
9	10	4.1641505936373875
9	11	16.53656200075767
9	12	17.479601187794458
9	13	2.630158776797437
9	14	16.61094862353866
9	15	0.003615223978375326
9	16	20.20862795501151
9	17	20.182454338711846
9	18	20.502789552384392
9	19	15.380061941526531
9	20	19.094491989269905
9	21	9.989282531194293
9	22	20.287526382514386
9	23	17.93528723011017
9	24	20.117529166178624
9	25	18.572999113065144
9	26	-8.342641960213983
9	27	-8.365331490571592
9	28	-7.968095816415145
9	29	-7.375596673900995
9	30	-7.047384836385435
9	31	-6.685781475427335
9	32	-6.306347514030591
9	33	-5.888340307818099
9	34	-5.476645919773171
9	35	-5.214285152955622
9	36	-4.792849052707998
9	37	-5.06639612554979
9	38	-4.688769735333363
9	39	-4.008448792437388
9	40	-3.677120665115864
10	1	6.306723113820954
10	2	5.5696500544940015
10	3	11.554326279296266
10	4	10.253910132151429
10	5	7.089521259719344
10	6	12.362222391454084
10	7	11.4397895788352
10	8	13.059481503224
10	9	10.818697770483485
10	10	12.856458285945081
10	11	8.613485077680746
10	12	6.864216572409841
10	13	6.245543392182054
10	14	6.918640931230968
10	15	5.157461377824529
10	16	10.516320262703818
10	17	9.951685107942616
10	18	10.579712629307469
10	19	11.995446556911148
10	20	5.863722758500675
10	21	9.989282531194293
10	22	12.825987920975928
10	23	8.627594922417863
10	24	8.271375320024777
10	25	7.572999113065142
10	26	7.503511885939863
10	27	7.480822355582255
10	28	6.801134952815625
10	29	7.393634095329775
10	30	6.875692086691489
10	31	6.621910832264971
10	32	6.309037101354026
10	33	6.342428922951131
10	34	5.215661772534521
10	35	4.47802253935207
10	36	2.6686894088304633
10	37	2.9336038744502098
10	38	3.080461033897407
10	39	5.6838588998703035
10	40	3.1690331810379826
12	1	2.1614239685218095
12	2	5.159393644237593
12	3	7.2124459374159215
12	4	6.262457140698441
12	5	2.6536238238219063
12	6	2.439145468377161
12	7	-2.517475378429758
12	8	4.546660990403485
12	9	-1.2069432551575403
12	10	9.164150593637387
12	11	12.203228667424337
12	12	10.70182341001668
12	13	8.852380999019658
12	14	4.833170845760883
12	15	6.892504112867265
12	16	11.87529462167818
12	17	11.404676560934067
12	18	11.725011774606614
12	19	10.15783971930431
12	20	15.094491989269905
12	21	14.878171420083182
12	22	10.065304160292166
12	23	11.379731674554613
12	24	7.117529166178624
12	25	12.23966577973181
12	26	3.879580262008238
12	27	3.9680018427617405
12	28	7.476348628029298
12	29	9.513292214987894
12	30	3.063726274725676
12	31	2.3142185245726647
12	32	-1.5285697362528126
12	33	-2.2216736411514324
12	34	0.19002074689349513
12	35	-2.547618486288955
12	36	-3.570626830485776
12	37	-3.844173903327568
12	38	-3.4665475131111405
12	39	-0.5640043479929435
12	40	-2.454898442893642
13	1	7.716979524077363
13	2	2.826060310904257
13	3	1.9902237151937001
13	4	3.15134602958733
13	5	-0.013042842844757985
13	6	4.439145468377161
13	7	1.5936357326813528
13	8	2.8799943237368204
13	9	3.459723411509124
13	10	14.164150593637387
13	11	8.203228667424337
13	12	8.81293452112779
13	13	15.296825443464101
13	14	17.61094862353866
13	15	15.67028189064504
13	16	17.20862795501151
13	17	14.182454338711846
13	18	16.502789552384392
13	19	13.713395274859863
13	20	3.0944919892699048
13	21	2.989282531194293
13	22	8.62085971584772
13	23	6.601953896776838
13	24	4.784195832845292
13	25	5.9063324463984745
13	26	-0.3426419602139834
13	27	-3.6986648239049256
13	28	-1.6347624830818113
13	29	1.6244033260990047
13	30	0.9526151636145652
13	31	0.31421852457266475
13	32	0.6936524859694089
13	33	2.7783263588485667
13	34	1.523354080226829
13	35	4.452381513711044
13	36	2.2071509472920017
13	37	3.600270541116876
13	38	-4.688769735333363
13	39	-4.008448792437388
13	40	-3.677120665115864
15	1	10.383646190744031
15	2	-0.5072730224290751
15	3	16.323557048527032
15	4	9.484679362920662
15	5	17.320290490488574
15	6	10.439145468377161
15	7	6.593635732681353
15	8	5.2133276570701526
15	9	7.126390078175792
15	10	11.164150593637387
15	11	14.53656200075767
15	12	-0.5203988122055421
15	13	14.630158776797437
15	14	12.610948623538661
15	15	18.003615223978375
15	16	14.208627955011512
15	17	15.182454338711846
15	18	16.502789552384392
15	19	10.380061941526531
15	20	16.094491989269905
15	21	15.989282531194293
15	22	0.2875263825143879
15	23	14.93528723011017
15	24	16.117529166178624
15	25	8.572999113065142
15	26	10.657358039786017
15	27	-1.3653314905715916
15	28	10.031904183584855
15	29	14.624403326099005
15	30	3.952615163614565
15	31	11.314218524572665
15	32	19.69365248596941
15	33	19.111659692181902
15	34	6.523354080226829
15	35	17.785714847044378
15	36	4.207150947292002
15	37	20.93360387445021
15	38	21.311230264666637
15	39	-4.008448792437388
15	40	-3.677120665115864
16	1	6.716979524077363
16	2	-3.507273022429075
16	3	-5.343109618139634
16	4	-0.8486539704126699
16	5	-2.3463761761780937
16	6	-0.560854531622839
16	7	2.926969066014685
16	8	3.2133276570701526
16	9	2.459723411509124
16	10	2.1641505936373875
16	11	4.203228667424337
16	12	5.479601187794458
16	13	2.630158776797437
16	14	2.9442819568719933
16	15	0.6702818906450432
16	16	4.2086279550115115
16	17	5.182454338711846
16	18	5.8361228857177245
16	19	-0.6199380584734691
16	20	7.427825322603237
16	21	4.322615864527625
16	22	5.62085971584772
16	23	8.268620563443502
16	24	7.117529166178624
16	25	-0.4270008869348576
16	26	2.9906913731193505
16	27	2.9680018427617423
16	28	3.3652375169181887
16	29	3.9577366594323387
16	30	4.285948496947899
16	31	4.647551857905999
16	32	4.026985819302743
16	33	5.444993025515235
16	34	5.856687413560163
16	35	-1.2142851529556218
16	36	4.207150947292002
16	37	3.9336038744502098
16	38	4.311230264666637
16	39	-4.008448792437388
16	40	-3.677120665115864
17	1	5.740789047886889
17	2	5.921298406142352
17	3	5.323557048527032
17	4	4.556107934349235
17	5	1.6060047762028589
17	6	5.939145468377161
17	7	7.236492875538495
17	8	9.284756228498726
17	9	5.9121043638900765
17	10	4.949864879351672
17	11	5.607990572186242
17	12	5.836744044937316
17	13	8.27301591965458
17	14	7.039520052110088
17	15	8.78932950969266
17	16	6.351485097868654
17	17	12.253882910140419
17	18	11.717075266670108
17	19	11.951490512955104
17	20	9.951634846412762
17	21	5.7035682454800085
17	22	10.43038352537153
17	23	10.006715801538743
17	24	9.546100594750051
17	25	6.715856255922285
17	26	1.0859294683574454
17	27	4.634668509428408
17	28	4.8890470407277125
17	29	7.195831897527578
17	30	4.381186592185994
17	31	5.885647096001238
17	32	4.479366771683695
17	33	7.754516835039043
17	34	5.166211223083971
17	35	4.571429132758665
17	36	5.850008090149144
17	37	8.93360387445021
17	38	5.525515978952351
17	39	8.420122636134039
17	40	10.180022192026993
18	1	6.116979524077365
18	2	8.75939364423759
18	3	8.1902237151937
18	4	10.751346029587328
18	5	11.720290490488573
18	6	6.3724788017104945
18	7	10.993635732681351
18	8	12.879994323736817
18	9	10.259723411509125
18	10	11.897483926970722
18	11	9.269895334091004
18	12	6.212934521127792
18	13	7.4301587767974375
18	14	8.544281956871995
18	15	9.203615223978375
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109	26	7.657358039786017
109	27	8.301335176095076
109	28	4.531904183584855
109	29	5.124403326099005
109	30	6.952615163614565
109	31	4.980885191239331
109	32	5.193652485969409
109	33	4.444993025515235
109	34	4.856687413560163
109	35	6.452381513711044
109	36	10.207150947292002
109	37	8.266937207783542
109	38	10.14456359799997
109	39	10.991551207562612
109	40	11.656212668217467
110	1	-18.61635380925597
110	2	-15.507273022429075
110	3	-16.676442951472968
110	4	-18.515320637079338
110	5	-15.679709509511426
110	6	-10.560854531622839
110	7	-14.406364267318647
110	8	-15.786672342929847
110	9	-14.873609921824208
110	10	-12.835849406362613
110	11	-14.46343799924233
110	12	-13.520398812205542
110	13	-14.369841223202563
110	14	-14.389051376461339
110	15	-13.996384776021625
110	16	-13.791372044988488
110	17	-13.817545661288154
110	18	-13.497210447615608
110	19	-11.619938058473469
110	20	-12.905508010730095
110	21	-11.010717468805707
110	22	-10.712473617485612
110	23	-11.06471276988983
110	24	-10.882470833821376
110	25	-10.427000886934858
110	26	-8.342641960213983
110	27	-8.365331490571592
110	28	-7.968095816415145
110	29	-7.375596673900995
110	30	-7.047384836385435
110	31	-6.685781475427335
110	32	-6.306347514030591
110	33	-5.888340307818099
110	34	-5.476645919773171
110	35	-5.214285152955622
110	36	-4.792849052707998
110	37	-5.06639612554979
110	38	-4.688769735333363
110	39	-4.008448792437388
110	40	-3.677120665115864
111	1	-18.61635380925597
111	2	-15.507273022429075
111	3	-16.676442951472968
111	4	-18.515320637079338
111	5	-15.679709509511426
111	6	-10.560854531622839
111	7	-14.406364267318647
111	8	-15.786672342929847
111	9	-14.873609921824208
111	10	-12.835849406362613
111	11	-14.46343799924233
111	12	-13.520398812205542
111	13	-14.369841223202563
111	14	-14.389051376461339
111	15	-13.996384776021625
111	16	-13.791372044988488
111	17	-13.817545661288154
111	18	-13.497210447615608
111	19	-11.619938058473469
111	20	-12.905508010730095
111	21	-11.010717468805707
111	22	-10.712473617485612
111	23	-11.06471276988983
111	24	-10.882470833821376
111	25	-10.427000886934858
111	26	-8.342641960213983
111	27	-8.365331490571592
111	28	-7.968095816415145
111	29	-7.375596673900995
111	30	-7.047384836385435
111	31	-6.685781475427335
111	32	-6.306347514030591
111	33	-5.888340307818099
111	34	-5.476645919773171
111	35	-5.214285152955622
111	36	-4.792849052707998
111	37	-5.06639612554979
111	38	-4.688769735333363
111	39	-4.008448792437388
111	40	-3.677120665115864
112	1	-8.41635380925597
112	2	-7.707273022429074
112	3	-12.876442951472967
112	4	-10.115320637079337
112	5	-8.479709509511427
112	6	-5.960854531622839
112	7	-6.006364267318647
112	8	-6.586672342929848
112	9	-6.2736099218242085
112	10	-6.435849406362612
112	11	-7.263437999242331
112	12	-7.320398812205543
112	13	-7.7698412232025635
112	14	-7.389051376461339
112	15	-4.596384776021624
112	16	-3.191372044988489
112	17	-6.217545661288154
112	18	-3.2972104476156083
112	19	-3.0199380584734694
112	20	-2.705508010730096
112	21	-4.010717468805707
112	22	-1.9124736174856114
112	23	-2.66471276988983
112	24	-1.2824708338213764
112	25	-9.027000886934857
112	26	2.4573580397860173
112	27	2.0346685094284087
112	28	2.031904183584855
112	29	-0.37559667390099527
112	30	2.752615163614566
112	31	5.714218524572663
112	32	0.8936524859694082
112	33	4.7116596921819
112	34	6.123354080226829
112	35	3.1857148470443786
112	36	6.407150947292001
112	37	5.133603874450209
112	38	7.711230264666636
112	39	7.191551207562611
112	40	6.9228793348841355
116	1	7.383646190744031
116	2	-15.507273022429075
116	3	-16.676442951472968
116	4	-18.515320637079338
116	5	-15.679709509511426
116	6	-10.560854531622839
116	7	-14.406364267318647
116	8	-15.786672342929847
116	9	-14.873609921824208
116	10	-12.835849406362613
116	11	-14.46343799924233
116	12	-13.520398812205542
116	13	-14.369841223202563
116	14	-14.389051376461339
116	15	-13.996384776021625
116	16	-13.791372044988488
116	17	-13.817545661288154
116	18	-13.497210447615608
116	19	-11.619938058473469
116	20	-12.905508010730095
116	21	-11.010717468805707
116	22	-10.712473617485612
116	23	-11.06471276988983
116	24	-10.882470833821376
116	25	-10.427000886934858
116	26	-8.342641960213983
116	27	-8.365331490571592
116	28	-7.968095816415145
116	29	-7.375596673900995
116	30	-7.047384836385435
116	31	-6.685781475427335
116	32	-6.306347514030591
116	33	-5.888340307818099
116	34	-5.476645919773171
116	35	-5.214285152955622
116	36	-4.792849052707998
116	37	-5.06639612554979
116	38	-4.688769735333363
116	39	-4.008448792437388
116	40	-3.677120665115864
119	1	2.383646190744031
119	2	8.492726977570925
119	3	-2.6764429514729677
119	4	12.484679362920662
119	5	13.320290490488574
119	6	-10.560854531622839
119	7	-14.406364267318647
119	8	-15.786672342929847
119	9	-14.873609921824208
119	10	-12.835849406362613
119	11	-14.46343799924233
119	12	-13.520398812205542
119	13	-14.369841223202563
119	14	-14.389051376461339
119	15	-13.996384776021625
119	16	-13.791372044988488
119	17	-13.817545661288154
119	18	-13.497210447615608
119	19	-11.619938058473469
119	20	-12.905508010730095
119	21	-11.010717468805707
119	22	-10.712473617485612
119	23	-11.06471276988983
119	24	-10.882470833821376
119	25	-10.427000886934858
119	26	-8.342641960213983
119	27	-8.365331490571592
119	28	-7.968095816415145
119	29	-7.375596673900995
119	30	-7.047384836385435
119	31	-6.685781475427335
119	32	-6.306347514030591
119	33	-5.888340307818099
119	34	-5.476645919773171
119	35	-5.214285152955622
119	36	-4.792849052707998
119	37	-5.06639612554979
119	38	-4.688769735333363
119	39	-4.008448792437388
119	40	-3.677120665115864
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	814.0
5	52.0
6	76.0
7	99.0
8	54.0
9	99.0
10	112.0
11	135.0
12	154.0
13	165.0
14	183.0
15	202.0
16	200.0
17	198.0
18	183.0
19	212.0
20	198.0
21	231.0
22	219.0
23	243.0
24	209.0
25	172.0
26	167.0
27	119.0
28	108.0
29	85.0
30	46.0
31	22.0
32	13.0
33	10.0
34	2.0
35	1.0
36	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.09985346451748	0.7536110529621101	0.020933640360058615	0.1256018421603517
8	98.36854214599457	0.5647354110018824	0.418322526668061	0.6483999163354947
9	96.2133891213389	1.778242677824268	1.0878661087866108	0.9205020920502093
10	40.73996655518395	35.618729096989966	10.493311036789297	13.14799331103679
11	30.16304347826087	29.68227424749164	20.777591973244146	19.377090301003346
12	35.52163913861593	16.015053313819777	21.910934559899644	26.552372987664647
13	26.254180602006688	20.150501672240804	26.442307692307693	27.15301003344482
14	21.92725752508361	24.31020066889632	28.365384615384613	25.39715719063545
15	24.12207357859532	23.035117056856187	28.992474916387962	23.850334448160535
16	27.278428093645484	22.01086956521739	25.52257525083612	25.188127090301005
17	26.985785953177256	23.536789297658864	24.47742474916388	25.0
18	27.21571906354515	24.26839464882943	23.327759197324415	25.188127090301005
19	20.871028823900694	16.87355354512939	20.408163265306122	41.84725436566379
20	25.68979933110368	24.28929765886288	25.08361204013378	24.937290969899664
21	21.73913043478261	21.42558528428094	28.846153846153843	27.98913043478261
22	22.09448160535117	19.377090301003346	28.2190635451505	30.309364548494983
23	26.254180602006688	19.31438127090301	30.706521739130434	23.724916387959865
24	22.74247491638796	20.025083612040135	28.741638795986624	28.490802675585286
25	23.634079966506174	20.180029307096504	29.8932384341637	26.29265229223362
26	20.219039595619208	20.76663858466723	32.22409435551811	26.79022746419545
27	18.90027179594397	16.600459962366713	34.476270123353544	30.022998118335774
28	17.31855260405773	18.887262079062957	35.70382765111901	28.0903576657603
29	19.581151832460733	17.109947643979055	36.774869109947645	26.534031413612563
30	18.189438390611905	15.234702430846605	39.43839061190277	27.137468566638727
31	17.857891427373715	17.81597149444561	42.08761265981975	22.23852441836093
32	22.061898787118363	13.028021748222502	39.81597657883731	25.094102885821833
33	17.328595317725753	15.050167224080269	43.812709030100336	23.808528428093645
34	15.530936454849497	15.865384615384615	46.46739130434783	22.13628762541806
35	14.927869537946895	18.001254442818315	45.996236671545056	21.074639347689732
36	14.300648128789462	18.670290612586243	44.490905289567216	22.538155969057076
37	14.910079464659137	16.58301965704726	45.46214972814722	23.044751150146382
38	15.931423792598787	17.45766255488187	44.84633075475643	21.76458289776291
39	16.3252508361204	17.098662207357858	43.66638795986622	22.909698996655518
40	13.31521739130435	17.516722408026755	44.20986622073579	24.95819397993311
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	2.5
12	4.0
13	4.0
14	4.5
15	5.0
16	10.0
17	15.0
18	15.0
19	23.5
20	32.0
21	43.5
22	55.0
23	55.0
24	83.5
25	112.0
26	116.5
27	121.0
28	121.0
29	132.0
30	143.0
31	165.0
32	187.0
33	187.0
34	215.0
35	243.0
36	252.5
37	262.0
38	262.0
39	272.0
40	282.0
41	278.0
42	274.0
43	274.0
44	290.5
45	307.0
46	285.5
47	264.0
48	264.0
49	258.5
50	253.0
51	273.5
52	294.0
53	294.0
54	288.5
55	283.0
56	304.0
57	325.0
58	325.0
59	345.5
60	366.0
61	347.0
62	328.0
63	328.0
64	280.5
65	233.0
66	209.0
67	185.0
68	185.0
69	143.0
70	101.0
71	79.5
72	58.0
73	58.0
74	44.5
75	31.0
76	23.0
77	15.0
78	15.0
79	9.0
80	3.0
81	2.0
82	1.0
83	1.0
84	1.0
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.1463210702341137
8	0.06270903010033445
9	0.08361204013377926
10	0.0
11	0.0
12	0.020903010033444816
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.6479933110367893
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.1463210702341137
26	0.7525083612040134
27	0.020903010033444816
28	0.06270903010033445
29	0.18812709030100336
30	0.2508361204013378
31	0.2717391304347826
32	0.04180602006688963
33	0.0
34	0.0
35	0.020903010033444816
36	0.020903010033444816
37	0.04180602006688963
38	0.020903010033444816
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	4784.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.25167224080268
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.451541361721	90.90719063545151
2	2.4617431803060548	4.640468227424749
3	0.5988023952095809	1.69314381270903
4	0.22177866489243736	0.8361204013377926
5	0.0665335994677312	0.31354515050167225
6	0.04435573297848747	0.2508361204013378
7	0.022177866489243733	0.1463210702341137
8	0.022177866489243733	0.16722408026755853
9	0.022177866489243733	0.18812709030100336
>10	0.08871146595697493	0.8570234113712374
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGTACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	11	0.229933110367893	No Hit
AAGTACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	10	0.20903010033444816	No Hit
AAGTACGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGC	10	0.20903010033444816	No Hit
AAGTACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.20903010033444816	No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	9	0.18812709030100336	No Hit
AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	8	0.16722408026755853	No Hit
AAGTACGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT	7	0.1463210702341137	No Hit
AAGTACGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.1254180602006689	No Hit
AAGTACGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.1254180602006689	No Hit
AAGTACGGGCAGAGTCGATTTTTTTTTTTTTTTTTTTTTT	5	0.10451505016722408	No Hit
AAGTACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	5	0.10451505016722408	No Hit
AAGTACGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC	5	0.10451505016722408	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.020903010033444816	0.0	0.0	0.0	0.0
19	0.020903010033444816	0.0	0.0	0.0	0.0
20	0.020903010033444816	0.0	0.0	0.0	0.0
21	0.020903010033444816	0.0	0.0	0.0	0.0
22	0.020903010033444816	0.0	0.0	0.0	0.0
23	0.020903010033444816	0.0	0.0	0.0	0.0
24	0.020903010033444816	0.0	0.0	0.0	0.0
25	0.020903010033444816	0.0	0.0	0.0	0.0
26	0.020903010033444816	0.0	0.0	0.0	0.0
27	0.020903010033444816	0.0	0.0	0.0	0.0
28	0.020903010033444816	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGGCC	25	1.7411725E-4	34.000004	6
CGGGGAT	25	1.7411725E-4	34.000004	6
ACGGGTA	25	1.7411725E-4	34.000004	5
ACGGGGT	25	1.7411725E-4	34.000004	5
ACGGGAA	50	2.5283953E-10	34.000004	5
CGGGGGG	30	1.1976439E-5	34.0	6
CGGGGGC	20	0.0025199114	34.0	6
CGGGGAC	20	0.0025199114	34.0	6
TACGGGT	55	1.6370905E-11	34.0	4
TACGGGC	55	1.6370905E-11	34.0	4
CGGGATT	20	0.0025199114	34.0	6
CGGGAAG	30	1.1976439E-5	34.0	6
ACGGGGG	70	0.0	34.0	5
ACGGGGA	65	0.0	34.0	5
ACGGGAT	40	5.574111E-8	34.0	5
ACGGGAG	30	1.1976439E-5	34.0	5
AAGTACG	480	0.0	33.645832	1
AGTACGG	475	0.0	33.642105	2
GTACGGG	455	0.0	33.626373	3
TACGGGG	200	0.0	33.15	4
>>END_MODULE