FastQCFastQC Report
Fri 17 Jun 2016
SRR1525812_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525812_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000
Sequences flagged as poor quality0
Sequence length40
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT50.5No Hit
AAGTACGGGGGGCTGGTGAGATGGCCCCGTGGGTAAGAGC30.3No Hit
AAGTACGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.3No Hit
AAGTACGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTTT30.3No Hit
AAGTACGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.3No Hit
AAGTACGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT20.2No Hit
AAGTACGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.2No Hit
AAGTACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT20.2No Hit
AAGTACGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT20.2No Hit
AAGTACGGGGCAGAGTCGCCTTTTTTTTTTTTTTTTTTTT20.2No Hit
AAGTACGGGGCCTCTTTTTTTTTTTTTTTTTTTTTTTTTT20.2No Hit
AAGTACGGGTATCAGATCGATTTTTTTTTTTTTTTTTTTT20.2No Hit
AAGTACGGGGGGCTGGTCCGATGCCCCCGTGGGTAAGAGC20.2No Hit
AAGTACGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT20.2No Hit
AAGTACGGGAAGATTAAACGAGAGCCCCTTTTTTTTTTTT20.2No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG900.034.01
TACGGGG200.001662030434.04
TACGGGA352.3309076E-734.04
AGTACGG900.034.02
GTACGGG850.034.03
Produced by FastQC (version 0.11.5)