##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525812_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1000
Sequences flagged as poor quality	0
Sequence length	40
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	10.911	4.0	4.0	22.0	4.0	29.0
2	9.601	4.0	4.0	17.0	4.0	25.0
3	8.65	4.0	4.0	13.0	4.0	22.0
4	9.217	4.0	4.0	16.0	4.0	23.0
5	9.108	4.0	4.0	14.0	4.0	23.0
6	7.45	4.0	4.0	8.0	4.0	15.0
7	9.274	4.0	4.0	17.0	4.0	23.0
8	10.083	4.0	4.0	19.0	4.0	28.0
9	9.46	4.0	4.0	17.0	4.0	27.0
10	7.674	4.0	4.0	9.0	4.0	19.0
11	8.302	4.0	4.0	10.0	4.0	22.0
12	8.152	4.0	4.0	10.0	4.0	22.0
13	8.573	4.0	4.0	10.0	4.0	24.0
14	8.229	4.0	4.0	9.0	4.0	22.0
15	8.193	4.0	4.0	4.0	4.0	23.0
16	8.087	4.0	4.0	4.0	4.0	22.0
17	7.927	4.0	4.0	4.0	4.0	22.0
18	7.603	4.0	4.0	4.0	4.0	20.0
19	7.873	4.0	4.0	4.0	4.0	21.0
20	8.016	4.0	4.0	4.0	4.0	22.0
21	8.392	4.0	4.0	4.0	4.0	25.0
22	8.145	4.0	4.0	4.0	4.0	24.0
23	8.28	4.0	4.0	4.0	4.0	26.0
24	7.983	4.0	4.0	4.0	4.0	23.0
25	7.85	4.0	4.0	4.0	4.0	23.0
26	8.768	4.0	4.0	4.0	4.0	31.0
27	8.795	4.0	4.0	4.0	4.0	32.0
28	8.759	4.0	4.0	4.0	4.0	32.0
29	8.68	4.0	4.0	4.0	4.0	31.0
30	8.929	4.0	4.0	4.0	4.0	34.0
31	8.709	4.0	4.0	4.0	4.0	32.0
32	8.618	4.0	4.0	4.0	4.0	31.0
33	8.852	4.0	4.0	4.0	4.0	35.0
34	8.905	4.0	4.0	4.0	4.0	35.0
35	8.74	4.0	4.0	4.0	4.0	35.0
36	8.374	4.0	4.0	4.0	4.0	31.0
37	8.301	4.0	4.0	4.0	4.0	31.0
38	8.174	4.0	4.0	4.0	4.0	31.0
39	8.071	4.0	4.0	4.0	4.0	31.0
40	8.039	4.0	4.0	4.0	4.0	31.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-4.8402910052910055
1	2	-4.674470899470899
1	3	-4.337962962962962
1	4	-4.721164021164022
1	5	-3.6955026455026454
1	6	-2.5439153439153452
1	7	-4.463095238095239
1	8	-4.814021164021163
1	9	-4.703968253968252
1	10	-3.0924603174603176
1	11	-3.6863756613756617
1	12	-3.2556878306878314
1	13	-3.424470899470898
1	14	-3.723280423280423
1	15	-3.725925925925927
1	16	-3.4231481481481483
1	17	-3.1490740740740737
1	18	-2.807407407407407
1	19	-3.643518518518518
1	20	-3.0018518518518524
1	21	-4.807407407407407
1	22	-4.256481481481481
1	23	-4.007407407407406
1	24	-3.6814814814814802
1	25	-3.7712962962962955
1	26	-4.973148148148148
1	27	-4.682407407407409
1	28	-4.533333333333331
1	29	-4.326851851851851
1	30	-4.689814814814813
1	31	-4.612037037037037
1	32	-4.437037037037037
1	33	-4.640740740740741
1	34	-4.622222222222222
1	35	-4.527777777777779
1	36	-4.03425925925926
1	37	-3.889814814814814
1	38	-3.776851851851851
1	39	-3.643518518518518
1	40	-3.828703703703704
4	1	-4.8402910052910055
4	2	-4.674470899470899
4	3	-4.337962962962962
4	4	-4.721164021164022
4	5	-3.6955026455026454
4	6	-2.5439153439153452
4	7	-4.463095238095239
4	8	-4.814021164021163
4	9	-4.703968253968252
4	10	-3.0924603174603176
4	11	-3.6863756613756617
4	12	-3.2556878306878314
4	13	-3.424470899470898
4	14	-3.723280423280423
4	15	-3.725925925925927
4	16	-3.4231481481481483
4	17	-3.1490740740740737
4	18	-2.807407407407407
4	19	-3.643518518518518
4	20	-3.0018518518518524
4	21	-4.807407407407407
4	22	-4.256481481481481
4	23	-4.007407407407406
4	24	-3.6814814814814802
4	25	-3.7712962962962955
4	26	-4.973148148148148
4	27	-4.682407407407409
4	28	-4.533333333333331
4	29	-4.326851851851851
4	30	-4.689814814814813
4	31	-4.612037037037037
4	32	-4.437037037037037
4	33	-4.640740740740741
4	34	-4.622222222222222
4	35	-4.527777777777779
4	36	-4.03425925925926
4	37	-3.889814814814814
4	38	-3.776851851851851
4	39	-3.643518518518518
4	40	-3.828703703703704
8	1	-4.8402910052910055
8	2	-4.674470899470899
8	3	-4.337962962962962
8	4	-4.721164021164022
8	5	-3.6955026455026454
8	6	-2.5439153439153452
8	7	-4.463095238095239
8	8	-4.814021164021163
8	9	-4.703968253968252
8	10	-3.0924603174603176
8	11	-3.6863756613756617
8	12	-3.2556878306878314
8	13	-3.424470899470898
8	14	-3.723280423280423
8	15	-3.725925925925927
8	16	-3.4231481481481483
8	17	-3.1490740740740737
8	18	-2.807407407407407
8	19	-3.643518518518518
8	20	-3.0018518518518524
8	21	-4.807407407407407
8	22	-4.256481481481481
8	23	-4.007407407407406
8	24	-3.6814814814814802
8	25	-3.7712962962962955
8	26	-4.973148148148148
8	27	-4.682407407407409
8	28	-4.533333333333331
8	29	-4.326851851851851
8	30	-4.689814814814813
8	31	-4.612037037037037
8	32	-4.437037037037037
8	33	-4.640740740740741
8	34	-4.622222222222222
8	35	-4.527777777777779
8	36	-4.03425925925926
8	37	-3.889814814814814
8	38	-3.776851851851851
8	39	-3.643518518518518
8	40	-3.828703703703704
10	1	-4.8402910052910055
10	2	-4.674470899470899
10	3	-4.337962962962962
10	4	-4.721164021164022
10	5	-3.6955026455026454
10	6	-2.5439153439153452
10	7	-4.463095238095239
10	8	-4.814021164021163
10	9	-4.703968253968252
10	10	-3.0924603174603176
10	11	-3.6863756613756617
10	12	-3.2556878306878314
10	13	-3.424470899470898
10	14	-3.723280423280423
10	15	-3.725925925925927
10	16	-3.4231481481481483
10	17	-3.1490740740740737
10	18	-2.807407407407407
10	19	-3.643518518518518
10	20	-3.0018518518518524
10	21	-4.807407407407407
10	22	-4.256481481481481
10	23	-4.007407407407406
10	24	-3.6814814814814802
10	25	-3.7712962962962955
10	26	-4.973148148148148
10	27	-4.682407407407409
10	28	-4.533333333333331
10	29	-4.326851851851851
10	30	-4.689814814814813
10	31	-4.612037037037037
10	32	-4.437037037037037
10	33	-4.640740740740741
10	34	-4.622222222222222
10	35	-4.527777777777779
10	36	-4.03425925925926
10	37	-3.889814814814814
10	38	-3.776851851851851
10	39	-3.643518518518518
10	40	-3.828703703703704
15	1	-4.8402910052910055
15	2	-4.674470899470899
15	3	-4.337962962962962
15	4	-4.721164021164022
15	5	-3.6955026455026454
15	6	-2.5439153439153452
15	7	-4.463095238095239
15	8	-4.814021164021163
15	9	-4.703968253968252
15	10	-3.0924603174603176
15	11	-3.6863756613756617
15	12	-3.2556878306878314
15	13	-3.424470899470898
15	14	-3.723280423280423
15	15	-3.725925925925927
15	16	-3.4231481481481483
15	17	-3.1490740740740737
15	18	-2.807407407407407
15	19	-3.643518518518518
15	20	-3.0018518518518524
15	21	-4.807407407407407
15	22	-4.256481481481481
15	23	-4.007407407407406
15	24	-3.6814814814814802
15	25	-3.7712962962962955
15	26	-4.973148148148148
15	27	-4.682407407407409
15	28	-4.533333333333331
15	29	-4.326851851851851
15	30	-4.689814814814813
15	31	-4.612037037037037
15	32	-4.437037037037037
15	33	-4.640740740740741
15	34	-4.622222222222222
15	35	-4.527777777777779
15	36	-4.03425925925926
15	37	-3.889814814814814
15	38	-3.776851851851851
15	39	-3.643518518518518
15	40	-3.828703703703704
17	1	16.159708994708993
17	2	16.325529100529103
17	3	14.662037037037038
17	4	12.778835978835978
17	5	17.804497354497354
17	6	4.456084656084655
17	7	14.536904761904761
17	8	20.185978835978837
17	9	16.296031746031748
17	10	11.907539682539682
17	11	11.813624338624338
17	12	5.744312169312169
17	13	11.075529100529103
17	14	4.776719576719577
17	15	10.774074074074072
17	16	12.576851851851853
17	17	9.850925925925926
17	18	17.692592592592593
17	19	16.35648148148148
17	20	20.998148148148147
17	21	23.192592592592593
17	22	22.24351851851852
17	23	26.492592592592594
17	24	21.31851851851852
17	25	25.728703703703705
17	26	22.026851851851852
17	27	13.817592592592591
17	28	22.96666666666667
17	29	16.67314814814815
17	30	25.310185185185187
17	31	7.387962962962963
17	32	3.0629629629629633
17	33	7.359259259259259
17	34	7.377777777777778
17	35	7.972222222222221
17	36	9.96574074074074
17	37	1.6101851851851858
17	38	10.223148148148148
17	39	10.356481481481481
17	40	10.171296296296296
18	1	4.1597089947089945
18	2	7.825529100529101
18	3	4.162037037037038
18	4	6.278835978835978
18	5	8.804497354497354
18	6	2.9560846560846548
18	7	4.536904761904761
18	8	7.685978835978837
18	9	6.796031746031748
18	10	3.9075396825396824
18	11	-1.1863756613756617
18	12	2.2443121693121686
18	13	5.575529100529102
18	14	8.776719576719577
18	15	3.274074074074073
18	16	4.576851851851852
18	17	8.350925925925926
18	18	2.692592592592593
18	19	5.356481481481482
18	20	4.998148148148148
18	21	12.192592592592593
18	22	11.243518518518519
18	23	11.492592592592594
18	24	10.31851851851852
18	25	6.2287037037037045
18	26	10.526851851851852
18	27	12.317592592592591
18	28	10.966666666666669
18	29	11.17314814814815
18	30	12.310185185185187
18	31	12.387962962962963
18	32	12.562962962962963
18	33	12.35925925925926
18	34	12.377777777777778
18	35	12.472222222222221
18	36	13.46574074074074
18	37	13.610185185185186
18	38	13.723148148148148
18	39	13.856481481481481
18	40	13.671296296296296
23	1	3.6597089947089945
23	2	-0.174470899470899
23	3	8.162037037037038
23	4	2.7788359788359784
23	5	2.3044973544973546
23	6	2.9560846560846548
23	7	4.036904761904761
23	8	6.685978835978837
23	9	6.796031746031748
23	10	3.4075396825396824
23	11	5.813624338624338
23	12	0.2443121693121686
23	13	4.575529100529102
23	14	6.276719576719577
23	15	7.774074074074073
23	16	8.076851851851853
23	17	9.850925925925926
23	18	9.692592592592593
23	19	9.356481481481481
23	20	6.498148148148148
23	21	3.692592592592593
23	22	7.743518518518519
23	23	7.992592592592594
23	24	8.31851851851852
23	25	8.228703703703705
23	26	6.526851851851852
23	27	6.817592592592591
23	28	8.466666666666669
23	29	8.67314814814815
23	30	-4.689814814814813
23	31	-4.612037037037037
23	32	-4.437037037037037
23	33	-4.640740740740741
23	34	-4.622222222222222
23	35	-4.527777777777779
23	36	-4.03425925925926
23	37	-3.889814814814814
23	38	-3.776851851851851
23	39	-3.643518518518518
23	40	-3.828703703703704
24	1	-4.8402910052910055
24	2	-4.674470899470899
24	3	-4.337962962962962
24	4	-4.721164021164022
24	5	-3.6955026455026454
24	6	-2.5439153439153452
24	7	-4.463095238095239
24	8	-4.814021164021163
24	9	-4.703968253968252
24	10	-3.0924603174603176
24	11	-3.6863756613756617
24	12	-3.2556878306878314
24	13	-3.424470899470898
24	14	-3.723280423280423
24	15	-3.725925925925927
24	16	-3.4231481481481483
24	17	-3.1490740740740737
24	18	-2.807407407407407
24	19	-3.643518518518518
24	20	-3.0018518518518524
24	21	-4.807407407407407
24	22	-4.256481481481481
24	23	-4.007407407407406
24	24	-3.6814814814814802
24	25	-3.7712962962962955
24	26	-4.973148148148148
24	27	-4.682407407407409
24	28	-4.533333333333331
24	29	-4.326851851851851
24	30	-4.689814814814813
24	31	-4.612037037037037
24	32	-4.437037037037037
24	33	-4.640740740740741
24	34	-4.622222222222222
24	35	-4.527777777777779
24	36	-4.03425925925926
24	37	-3.889814814814814
24	38	-3.776851851851851
24	39	-3.643518518518518
24	40	-3.828703703703704
26	1	2.6597089947089945
26	2	7.825529100529101
26	3	4.662037037037038
26	4	6.278835978835978
26	5	2.8044973544973546
26	6	0.9560846560846548
26	7	3.0369047619047613
26	8	4.685978835978837
26	9	5.296031746031748
26	10	6.407539682539682
26	11	1.3136243386243383
26	12	6.244312169312169
26	13	6.075529100529102
26	14	5.276719576719577
26	15	7.774074074074073
26	16	8.076851851851853
26	17	2.3509259259259263
26	18	7.692592592592593
26	19	8.856481481481481
26	20	8.998148148148147
26	21	0.1925925925925931
26	22	-2.2564814814814813
26	23	5.992592592592594
26	24	4.31851851851852
26	25	1.7287037037037045
26	26	4.026851851851852
26	27	3.3175925925925913
26	28	-0.5333333333333314
26	29	-1.8268518518518508
26	30	6.310185185185187
26	31	3.3879629629629626
26	32	7.062962962962963
26	33	3.359259259259259
26	34	-2.122222222222222
26	35	3.4722222222222214
26	36	11.46574074074074
26	37	11.610185185185186
26	38	11.723148148148148
26	39	11.856481481481481
26	40	11.671296296296296
27	1	-4.8402910052910055
27	2	-4.674470899470899
27	3	-4.337962962962962
27	4	-4.721164021164022
27	5	-3.6955026455026454
27	6	-2.5439153439153452
27	7	-4.463095238095239
27	8	-4.814021164021163
27	9	-4.703968253968252
27	10	-3.0924603174603176
27	11	-3.6863756613756617
27	12	-3.2556878306878314
27	13	-3.424470899470898
27	14	-3.723280423280423
27	15	-3.725925925925927
27	16	-3.4231481481481483
27	17	-3.1490740740740737
27	18	-2.807407407407407
27	19	-3.643518518518518
27	20	-3.0018518518518524
27	21	-4.807407407407407
27	22	-4.256481481481481
27	23	-4.007407407407406
27	24	-3.6814814814814802
27	25	-3.7712962962962955
27	26	-4.973148148148148
27	27	-4.682407407407409
27	28	-4.533333333333331
27	29	-4.326851851851851
27	30	-4.689814814814813
27	31	-4.612037037037037
27	32	-4.437037037037037
27	33	-4.640740740740741
27	34	-4.622222222222222
27	35	-4.527777777777779
27	36	-4.03425925925926
27	37	-3.889814814814814
27	38	-3.776851851851851
27	39	-3.643518518518518
27	40	-3.828703703703704
33	1	-4.8402910052910055
33	2	-4.674470899470899
33	3	-4.337962962962962
33	4	-4.721164021164022
33	5	-3.6955026455026454
33	6	-2.5439153439153452
33	7	-4.463095238095239
33	8	-4.814021164021163
33	9	-4.703968253968252
33	10	-3.0924603174603176
33	11	-3.6863756613756617
33	12	-3.2556878306878314
33	13	-3.424470899470898
33	14	-3.723280423280423
33	15	-3.725925925925927
33	16	-3.4231481481481483
33	17	-3.1490740740740737
33	18	-2.807407407407407
33	19	-3.643518518518518
33	20	-3.0018518518518524
33	21	-4.807407407407407
33	22	-4.256481481481481
33	23	-4.007407407407406
33	24	-3.6814814814814802
33	25	-3.7712962962962955
33	26	-4.973148148148148
33	27	-4.682407407407409
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107	12	-3.2556878306878314
107	13	-3.424470899470898
107	14	-3.723280423280423
107	15	-3.725925925925927
107	16	-3.4231481481481483
107	17	-3.1490740740740737
107	18	-2.807407407407407
107	19	-3.643518518518518
107	20	-3.0018518518518524
107	21	-4.807407407407407
107	22	-4.256481481481481
107	23	-4.007407407407406
107	24	-3.6814814814814802
107	25	-3.7712962962962955
107	26	-4.973148148148148
107	27	-4.682407407407409
107	28	-4.533333333333331
107	29	-4.326851851851851
107	30	-4.689814814814813
107	31	-4.612037037037037
107	32	-4.437037037037037
107	33	-4.640740740740741
107	34	-4.622222222222222
107	35	-4.527777777777779
107	36	-4.03425925925926
107	37	-3.889814814814814
107	38	-3.776851851851851
107	39	-3.643518518518518
107	40	-3.828703703703704
108	1	0.28470899470899447
108	2	-0.799470899470899
108	3	-0.9629629629629619
108	4	-1.7211640211640216
108	5	-0.9455026455026454
108	6	-0.04391534391534524
108	7	-0.33809523809523867
108	8	1.1859788359788368
108	9	1.0460317460317476
108	10	-0.7174603174603176
108	11	0.6886243386243383
108	12	1.3693121693121686
108	13	1.950529100529102
108	14	1.526719576719577
108	15	0.7740740740740728
108	16	0.9518518518518517
108	17	2.2259259259259263
108	18	1.442592592592593
108	19	-0.7685185185185182
108	20	0.24814814814814756
108	21	0.4425925925925931
108	22	1.3685185185185187
108	23	0.9925925925925938
108	24	1.8185185185185198
108	25	0.8537037037037045
108	26	0.6518518518518519
108	27	1.9425925925925913
108	28	1.2166666666666686
108	29	2.798148148148149
108	30	1.185185185185187
108	31	2.5129629629629626
108	32	2.5629629629629633
108	33	2.109259259259259
108	34	2.377777777777778
108	35	1.7222222222222214
108	36	-0.15925925925925988
108	37	-0.01481481481481417
108	38	0.09814814814814898
108	39	0.23148148148148184
108	40	0.046296296296295836
109	1	1.1597089947089945
109	2	1.325529100529101
109	3	1.3287037037037042
109	4	0.6121693121693124
109	5	-3.3621693121693124
109	6	1.1227513227513217
109	7	1.5369047619047613
109	8	3.5193121693121707
109	9	2.9626984126984137
109	10	-0.7591269841269845
109	11	2.9802910052910043
109	12	-0.5890211640211644
109	13	0.9088624338624358
109	14	3.276719576719577
109	15	0.2740740740740728
109	16	4.243518518518518
109	17	1.1842592592592602
109	18	2.525925925925927
109	19	1.6898148148148158
109	20	0.6648148148148145
109	21	4.192592592592593
109	22	4.743518518518519
109	23	6.325925925925928
109	24	6.651851851851854
109	25	6.5620370370370384
109	26	5.360185185185186
109	27	5.650925925925925
109	28	5.8000000000000025
109	29	6.006481481481483
109	30	5.643518518518521
109	31	5.7212962962962965
109	32	5.896296296296297
109	33	5.692592592592593
109	34	5.711111111111112
109	35	5.805555555555555
109	36	-4.03425925925926
109	37	-3.889814814814814
109	38	-3.776851851851851
109	39	-3.643518518518518
109	40	-3.828703703703704
110	1	-4.8402910052910055
110	2	-4.674470899470899
110	3	-4.337962962962962
110	4	-4.721164021164022
110	5	-3.6955026455026454
110	6	-2.5439153439153452
110	7	-4.463095238095239
110	8	-4.814021164021163
110	9	-4.703968253968252
110	10	-3.0924603174603176
110	11	-3.6863756613756617
110	12	-3.2556878306878314
110	13	-3.424470899470898
110	14	-3.723280423280423
110	15	-3.725925925925927
110	16	-3.4231481481481483
110	17	-3.1490740740740737
110	18	-2.807407407407407
110	19	-3.643518518518518
110	20	-3.0018518518518524
110	21	-4.807407407407407
110	22	-4.256481481481481
110	23	-4.007407407407406
110	24	-3.6814814814814802
110	25	-3.7712962962962955
110	26	-4.973148148148148
110	27	-4.682407407407409
110	28	-4.533333333333331
110	29	-4.326851851851851
110	30	-4.689814814814813
110	31	-4.612037037037037
110	32	-4.437037037037037
110	33	-4.640740740740741
110	34	-4.622222222222222
110	35	-4.527777777777779
110	36	-4.03425925925926
110	37	-3.889814814814814
110	38	-3.776851851851851
110	39	-3.643518518518518
110	40	-3.828703703703704
115	1	-4.8402910052910055
115	2	-4.674470899470899
115	3	-4.337962962962962
115	4	-4.721164021164022
115	5	-3.6955026455026454
115	6	-2.5439153439153452
115	7	-4.463095238095239
115	8	-4.814021164021163
115	9	-4.703968253968252
115	10	-3.0924603174603176
115	11	-3.6863756613756617
115	12	-3.2556878306878314
115	13	-3.424470899470898
115	14	-3.723280423280423
115	15	-3.725925925925927
115	16	-3.4231481481481483
115	17	-3.1490740740740737
115	18	-2.807407407407407
115	19	-3.643518518518518
115	20	-3.0018518518518524
115	21	-4.807407407407407
115	22	-4.256481481481481
115	23	-4.007407407407406
115	24	-3.6814814814814802
115	25	-3.7712962962962955
115	26	-4.973148148148148
115	27	-4.682407407407409
115	28	-4.533333333333331
115	29	-4.326851851851851
115	30	-4.689814814814813
115	31	-4.612037037037037
115	32	-4.437037037037037
115	33	-4.640740740740741
115	34	-4.622222222222222
115	35	-4.527777777777779
115	36	-4.03425925925926
115	37	-3.889814814814814
115	38	-3.776851851851851
115	39	-3.643518518518518
115	40	-3.828703703703704
120	1	-4.8402910052910055
120	2	-4.674470899470899
120	3	-4.337962962962962
120	4	-4.721164021164022
120	5	-3.6955026455026454
120	6	-2.5439153439153452
120	7	-4.463095238095239
120	8	-4.814021164021163
120	9	-4.703968253968252
120	10	-3.0924603174603176
120	11	-3.6863756613756617
120	12	-3.2556878306878314
120	13	-3.424470899470898
120	14	-3.723280423280423
120	15	-3.725925925925927
120	16	-3.4231481481481483
120	17	-3.1490740740740737
120	18	-2.807407407407407
120	19	-3.643518518518518
120	20	-3.0018518518518524
120	21	-4.807407407407407
120	22	-4.256481481481481
120	23	-4.007407407407406
120	24	-3.6814814814814802
120	25	-3.7712962962962955
120	26	-4.973148148148148
120	27	-4.682407407407409
120	28	-4.533333333333331
120	29	-4.326851851851851
120	30	-4.689814814814813
120	31	-4.612037037037037
120	32	-4.437037037037037
120	33	-4.640740740740741
120	34	-4.622222222222222
120	35	-4.527777777777779
120	36	-4.03425925925926
120	37	-3.889814814814814
120	38	-3.776851851851851
120	39	-3.643518518518518
120	40	-3.828703703703704
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	672.0
5	13.0
6	22.0
7	6.0
8	11.0
9	16.0
10	7.0
11	8.0
12	11.0
13	16.0
14	11.0
15	7.0
16	11.0
17	8.0
18	8.0
19	17.0
20	19.0
21	19.0
22	19.0
23	22.0
24	25.0
25	20.0
26	14.0
27	11.0
28	4.0
29	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	94.84848484848484	0.30303030303030304	1.2121212121212122	3.6363636363636362
8	92.30000000000001	2.0	3.0	2.7
9	91.60000000000001	3.6999999999999997	2.7	2.0
10	36.1	35.699999999999996	11.5	16.7
11	27.500000000000004	24.2	23.400000000000002	24.9
12	33.2	14.2	23.799999999999997	28.799999999999997
13	19.400000000000002	19.8	30.2	30.599999999999998
14	18.91891891891892	23.923923923923923	32.532532532532535	24.624624624624623
15	23.599999999999998	22.0	31.4	23.0
16	26.0	23.7	26.400000000000002	23.9
17	20.200000000000003	21.9	32.0	25.900000000000002
18	18.6	22.1	28.199999999999996	31.1
19	13.841524573721165	18.655967903711137	27.482447342026077	40.02006018054162
20	19.5	20.9	31.3	28.299999999999997
21	14.2	26.6	34.2	25.0
22	15.4	23.799999999999997	35.699999999999996	25.1
23	14.2	22.1	35.9	27.800000000000004
24	16.0	24.7	33.5	25.8
25	15.446339017051153	21.76529588766299	31.293881644934807	31.49448345035105
26	11.122244488977955	21.24248496993988	33.366733466933866	34.2685370741483
27	13.165829145728644	19.396984924623116	35.678391959798994	31.758793969849247
28	10.62124248496994	21.8436873747495	34.46893787575151	33.06613226452906
29	15.461847389558233	18.373493975903614	35.6425702811245	30.522088353413658
30	14.7	18.4	41.3	25.6
31	14.714714714714713	17.61761761761762	38.33833833833834	29.32932932932933
32	16.8	16.3	40.2	26.700000000000003
33	15.7	16.8	41.4	26.1
34	11.3	18.7	48.6	21.4
35	11.899999999999999	19.2	45.4	23.5
36	11.700000000000001	20.7	44.800000000000004	22.8
37	13.467336683417086	18.79396984924623	44.42211055276382	23.316582914572866
38	10.8	19.5	46.400000000000006	23.3
39	13.700000000000001	18.0	44.1	24.2
40	11.4	18.8	43.5	26.3
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	1.0
12	1.0
13	1.0
14	2.0
15	3.0
16	6.5
17	10.0
18	10.0
19	15.5
20	21.0
21	27.0
22	33.0
23	33.0
24	35.0
25	37.0
26	35.5
27	34.0
28	34.0
29	33.5
30	33.0
31	37.0
32	41.0
33	41.0
34	42.0
35	43.0
36	43.5
37	44.0
38	44.0
39	50.0
40	56.0
41	55.5
42	55.0
43	55.0
44	58.5
45	62.0
46	67.0
47	72.0
48	72.0
49	72.0
50	72.0
51	77.5
52	83.0
53	83.0
54	65.5
55	48.0
56	46.5
57	45.0
58	45.0
59	49.0
60	53.0
61	47.0
62	41.0
63	41.0
64	41.0
65	41.0
66	30.5
67	20.0
68	20.0
69	18.0
70	16.0
71	16.5
72	17.0
73	17.0
74	12.5
75	8.0
76	6.0
77	4.0
78	4.0
79	4.5
80	5.0
81	2.5
82	0.0
83	0.0
84	0.5
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.1
15	0.0
16	0.0
17	0.0
18	0.0
19	0.3
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.3
26	0.2
27	0.5
28	0.2
29	0.4
30	0.0
31	0.1
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.5
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	1000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.8
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.46625766871165	96.3
2	1.0224948875255624	2.0
3	0.408997955010225	1.2
4	0.0	0.0
5	0.10224948875255625	0.5
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGTACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.5	No Hit
AAGTACGGGGGGCTGGTGAGATGGCCCCGTGGGTAAGAGC	3	0.3	No Hit
AAGTACGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.3	No Hit
AAGTACGGGCGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	3	0.3	No Hit
AAGTACGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.3	No Hit
AAGTACGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT	2	0.2	No Hit
AAGTACGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.2	No Hit
AAGTACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	2	0.2	No Hit
AAGTACGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.2	No Hit
AAGTACGGGGCAGAGTCGCCTTTTTTTTTTTTTTTTTTTT	2	0.2	No Hit
AAGTACGGGGCCTCTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.2	No Hit
AAGTACGGGTATCAGATCGATTTTTTTTTTTTTTTTTTTT	2	0.2	No Hit
AAGTACGGGGGGCTGGTCCGATGCCCCCGTGGGTAAGAGC	2	0.2	No Hit
AAGTACGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.2	No Hit
AAGTACGGGAAGATTAAACGAGAGCCCCTTTTTTTTTTTT	2	0.2	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	90	0.0	34.0	1
TACGGGG	20	0.0016620304	34.0	4
TACGGGA	35	2.3309076E-7	34.0	4
AGTACGG	90	0.0	34.0	2
GTACGGG	85	0.0	34.0	3
>>END_MODULE