SRR1525810_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1525810_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	62649
Sequences flagged as poor quality	0
Sequence length	56
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATCAACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	764	1.2194927293332694	No Hit
ATCAACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	547	0.8731184855304953	No Hit
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	303	0.4836469855863621	No Hit
ATCAACGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	241	0.3846829159284266	No Hit
ATCAACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	229	0.3655285798656004	No Hit
ATCAACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	186	0.2968922089738064	No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	180	0.2873150409423933	No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	176	0.28093026225478457	No Hit
ATCAACGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	138	0.22027486472250155	No Hit
ATCAACGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	130	0.20750530734728406	No Hit
ATCAACGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	124	0.197928139315871	No Hit
ATCAACGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	119	0.18994716595636005	No Hit
ATCAACGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	115	0.1835623872687513	No Hit
ATCAACGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	109	0.17398521923733817	No Hit
ATCAACGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	105	0.16760044054972945	No Hit
ATCAACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	100	0.15961946719021852	No Hit
ATCAACGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC	95	0.1516384938307076	No Hit
ATCAACGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT	93	0.1484461044869032	No Hit
ATCAACGGGGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA	84	0.13408035243978356	No Hit
ATCAACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	83	0.13248415776788136	No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	83	0.13248415776788136	No Hit
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	80	0.1276955737521748	No Hit
ATCAACGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA	79	0.12609937908027263	No Hit
ATCAACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	68	0.10854123768934858	No Hit
ATCAACGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG	64	0.10215645900173984	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGAA	15	0.007846355	50.85948	41
TGTCGAC	15	0.007846355	50.85948	41
CGCATTG	35	4.587855E-8	50.776512	39
TTCACTC	15	0.00789722	50.776512	40
GCGCATT	35	4.587855E-8	50.776512	38
GCGGATC	30	9.275627E-7	50.776512	38
GCGGATA	15	0.00789722	50.776512	38
GCACTAA	15	0.00789722	50.776512	40
AAGAGAC	15	0.00794833	50.693813	35
TAAGACC	15	0.00794833	50.693813	35
GGCGCAT	35	4.647336E-8	50.69381	37
TGACCGA	15	0.008025455	50.57027	34
ACTTGGC	20	3.926865E-4	50.570267	34
TAAGAGA	20	3.926865E-4	50.570267	34
TCCGCCA	15	0.008286566	50.162773	45
GTGGTAC	20	4.08706E-4	50.16277	45
TGGTACA	20	4.08706E-4	50.16277	46
AGCGTGG	15	0.00831302	50.122383	42
CACTCCG	15	0.00831302	50.122383	42
TCACATA	15	0.008339536	50.08206	10

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content