##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525810_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	62649
Sequences flagged as poor quality	0
Sequence length	56
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.942473144024646	38.0	35.0	39.0	30.0	40.0
2	35.01382304585867	38.0	35.0	39.0	25.0	40.0
3	34.84347715047327	38.0	35.0	39.0	24.0	40.0
4	34.57555587479449	38.0	35.0	39.0	22.0	40.0
5	34.43676674807259	38.0	34.0	39.0	23.0	40.0
6	33.8864626729876	38.0	33.0	39.0	20.0	40.0
7	34.594550591390124	38.0	35.0	39.0	25.0	40.0
8	34.6776006001692	38.0	35.0	39.0	25.0	40.0
9	34.640568883781064	38.0	35.0	39.0	24.0	40.0
10	33.70896582547208	38.0	33.0	39.0	20.0	40.0
11	34.54784593529027	38.0	34.0	39.0	27.0	40.0
12	33.30011652221105	37.0	32.0	39.0	21.0	40.0
13	32.48163578029977	36.0	31.0	39.0	17.0	40.0
14	33.37327012402432	38.0	32.0	39.0	21.0	40.0
15	32.05144535427541	36.0	30.0	39.0	15.0	40.0
16	31.75311657009689	36.0	29.0	38.0	17.0	40.0
17	30.70465609985794	35.0	27.0	38.0	13.0	39.0
18	30.225302877938994	35.0	26.0	38.0	12.0	39.0
19	31.491452377531964	35.0	28.0	38.0	16.0	39.0
20	31.874970071349903	36.0	29.0	38.0	18.0	40.0
21	32.38487445928906	36.0	30.0	39.0	17.0	40.0
22	31.681814554103017	36.0	29.0	39.0	15.0	40.0
23	32.412648246580154	37.0	30.0	39.0	17.0	40.0
24	31.785998180338073	36.0	29.0	39.0	15.0	40.0
25	32.18716978722725	36.0	30.0	39.0	17.0	40.0
26	32.65517406502897	37.0	32.0	39.0	18.0	40.0
27	32.767961180545576	37.0	32.0	39.0	18.0	40.0
28	32.7261887659819	37.0	32.0	39.0	18.0	40.0
29	32.34773100927389	36.0	31.0	39.0	16.0	40.0
30	32.074861530112216	36.0	31.0	39.0	15.0	40.0
31	32.664384108285844	36.0	32.0	39.0	17.0	40.0
32	32.61653019202222	37.0	32.0	39.0	17.0	40.0
33	32.39441970342703	36.0	32.0	39.0	15.0	39.0
34	32.205510064007406	36.0	31.0	39.0	13.0	39.0
35	32.161502976903066	36.0	31.0	39.0	10.0	40.0
36	31.230825711503776	35.0	30.0	38.0	4.0	39.0
37	31.04273013136682	35.0	30.0	38.0	4.0	39.0
38	30.424252581844883	35.0	29.0	38.0	4.0	39.0
39	29.357611454292964	35.0	27.0	38.0	4.0	39.0
40	27.221184695685487	33.0	18.0	38.0	4.0	39.0
41	25.383773084965444	31.0	13.0	36.0	4.0	39.0
42	25.971539848999985	31.0	17.0	36.0	4.0	39.0
43	26.102459735989402	31.0	17.0	36.0	4.0	39.0
44	25.376430589474694	31.0	13.0	36.0	4.0	39.0
45	26.082475378697186	31.0	18.0	36.0	4.0	39.0
46	24.18831904739102	29.0	10.0	35.0	4.0	39.0
47	23.343213778352407	28.0	5.0	35.0	4.0	39.0
48	23.82261488611151	28.0	4.0	35.0	4.0	39.0
49	22.903972928538366	27.0	4.0	35.0	4.0	39.0
50	23.49185142619994	28.0	4.0	35.0	4.0	39.0
51	23.637839390892115	29.0	4.0	35.0	4.0	39.0
52	23.337419591693404	29.0	4.0	35.0	4.0	39.0
53	23.20402560296254	29.0	4.0	35.0	4.0	39.0
54	22.83602292135549	29.0	4.0	35.0	4.0	38.0
55	22.58374435346135	29.0	4.0	35.0	4.0	38.0
56	21.734648597742982	27.0	4.0	35.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	4.189647081647436
1	2	5.424139872779932
1	3	5.197177660212077
1	4	5.465419974710237
1	5	5.937780941973546
1	6	5.640248962874754
1	7	5.025342345449609
1	8	5.03907094875602
1	9	4.730691594983966
1	10	5.603627591970703
1	11	-0.6655536715707413
1	12	-8.930661094124044
1	13	-19.196250853443303
1	14	-9.72172886230468
1	15	0.3276676398785483
1	16	-2.1561136148625657
1	17	-7.686986769925774
1	18	-5.867206947169475
1	19	-0.7120164972233027
1	20	-1.7591539300056063
1	21	5.882748062296606
1	22	6.789461219841883
1	23	6.160767946991836
1	24	6.601494772088937
1	25	6.216021671043979
1	26	4.8664995294087525
1	27	4.824455123982883
1	28	4.929477841653462
1	29	5.233678180648802
1	30	5.071875432849769
1	31	4.023874366821115
1	32	4.474564483093747
1	33	3.800284340924179
1	34	4.441110966937746
1	35	4.018156894201844
1	36	3.5485446834489167
1	37	3.315209329971605
1	38	3.9522151127230494
1	39	1.6911890520162842
1	40	-12.722999470099195
1	41	-2.758445228569741
1	42	-4.939027566576666
1	43	-10.568994513584816
1	44	-15.039557925128765
1	45	-5.262249283572409
1	46	-7.402691620898022
1	47	-10.738017964807224
1	48	-6.722794351284007
1	49	-13.38984849585724
1	50	-5.744344866927911
1	51	-0.8626184863247026
1	52	-0.9785815714505439
1	53	-0.4821109655281255
1	54	-0.1974998566494719
1	55	-0.08001859485683482
1	56	2.5976538619692136
2	1	4.625234843322161
2	2	5.870194623182506
2	3	5.602572185171816
2	4	5.781845095483185
2	5	6.1805345651619525
2	6	6.692583906514049
2	7	5.634843150602592
2	8	5.570471914939596
2	9	5.305973398526639
2	10	5.943402149136563
2	11	3.228971288171607
2	12	-1.036941287360758
2	13	0.6382902093586296
2	14	4.508947466197739
2	15	5.196830280780322
2	16	4.6148203625931465
2	17	7.050935935388235
2	18	4.703646514988339
2	19	6.3310591871567325
2	20	5.2525207881908536
2	21	6.352152249092093
2	22	7.330926598263787
2	23	6.117289686122273
2	24	7.293523757596187
2	25	6.206359835295185
2	26	6.120525294304084
2	27	5.613907619957114
2	28	5.671828888352334
2	29	5.810570290149613
2	30	6.062616173590509
2	31	5.154711725919345
2	32	5.330442099840926
2	33	5.149318157349299
2	34	4.673397601398296
2	35	5.235145622060138
2	36	5.335581720485955
2	37	5.0692350948669365
2	38	6.176450217392933
2	39	6.7849893740774725
2	40	3.9670166329603873
2	41	8.582134481575189
2	42	7.399539261120598
2	43	6.884709190118887
2	44	-4.3257898091867375
2	45	7.020761988569301
2	46	6.697952501485233
2	47	4.21729604485461
2	48	6.885498724400371
2	49	-0.4949209596253539
2	50	6.920308917291091
2	51	9.527478132032783
2	52	9.196136625006783
2	53	9.045264235760119
2	54	9.031566165894812
2	55	8.806857411584389
2	56	8.569473507701904
3	1	4.232934648390383
3	2	5.553430490628905
3	3	5.721967896672794
3	4	6.103482522383764
3	5	6.399832810775987
3	6	6.087808078054007
3	7	5.567104359179588
3	8	5.104097645933749
3	9	5.233361312756664
3	10	5.818158484419211
3	11	4.194858227742756
3	12	4.549316022580761
3	13	5.4950153555574595
3	14	4.968011793683118
3	15	-0.5916687445608098
3	16	6.6255416101565
3	17	7.567017806733265
3	18	7.817194273272936
3	19	7.217998758306827
3	20	5.7417995406275
3	21	6.772717551236347
3	22	7.787554278575676
3	23	6.924307229981924
3	24	7.793523757596187
3	25	6.523611297283491
3	26	6.005515547715387
3	27	6.062250699879147
3	28	6.308768459502435
3	29	5.668270095217835
3	30	5.620121046884858
3	31	5.070403733716621
3	32	4.429369975084594
3	33	5.488499443899002
3	34	5.111019433756969
3	35	4.3589272984733896
3	36	6.7546850343261085
3	37	6.598962190383499
3	38	7.567775753455315
3	39	7.965788594350382
3	40	9.853468874675787
3	41	11.933011674557644
3	42	11.42634238002898
3	43	10.966093205713431
3	44	11.349648787304492
3	45	10.782945224436745
3	46	12.458673749048586
3	47	13.64565861795403
3	48	11.97419268151538
3	49	13.785780794760608
3	50	12.376936597602981
3	51	12.613735441974306
3	52	11.970503096741673
3	53	12.106180415097349
3	54	12.20066947973497
3	55	11.843894448621427
3	56	11.760506646103462
4	1	4.6519232964812005
4	2	6.0771663007424195
4	3	6.096580899768767
4	4	6.530211108555079
4	5	6.847201231828624
4	6	6.907725518714482
4	7	5.700747290036141
4	8	5.895635313632411
4	9	6.2302653375244645
4	10	6.691223499899092
4	11	4.659254512572481
4	12	5.847045640743815
4	13	5.572930732234443
4	14	5.858620668812115
4	15	6.362407622828247
4	16	7.632249556492933
4	17	9.307471883100657
4	18	9.772818628278099
4	19	8.68291103900858
4	20	7.529209241349893
4	21	7.705638087872053
4	22	8.58528389673873
4	23	7.734936744945802
4	24	7.391562973282458
4	25	7.4694317307200215
4	26	6.602006775785565
4	27	6.367720256122691
4	28	6.342308191184575
4	29	6.655886194289046
4	30	6.576777393634085
4	31	5.180310854459652
4	32	5.249287415745069
4	33	6.181997895911387
4	34	5.350957514252325
4	35	5.273271983715908
4	36	6.533838801095975
4	37	6.408559713603314
4	38	7.101831481009491
4	39	7.808409853380308
4	40	0.5939229510431758
4	41	11.738997226673224
4	42	10.467622049791625
4	43	10.01924078053283
4	44	10.95852391630346
4	45	10.198321901423327
4	46	11.837930714992858
4	47	12.718930031782715
4	48	12.668723125271836
4	49	11.877628059982488
4	50	12.164862294197409
4	51	12.303621922882456
4	52	12.438511352675626
4	53	12.663455956893017
4	54	12.371980109249932
4	55	11.98114935058221
4	56	11.238318823606043
5	1	4.5967012076456655
5	2	6.052356376772835
5	3	6.064968254710742
5	4	6.3181262746215054
5	5	6.789378102576922
5	6	6.797081261011403
5	7	5.861411555742421
5	8	5.5392927766176
5	9	6.0794049933868095
5	10	6.437922179370876
5	11	4.9107551128125735
5	12	5.826437397446497
5	13	6.20858499393913
5	14	5.754979212229479
5	15	7.259566486373668
5	16	7.607639712555358
5	17	8.101389450127467
5	18	9.381462085661052
5	19	7.322366821321509
5	20	7.412762662718439
5	21	7.3905120374518845
5	22	7.935423952761141
5	23	7.3315754004079885
5	24	7.541823077324079
5	25	7.217130810351872
5	26	6.003367320003253
5	27	5.251473757523254
5	28	5.236265774217788
5	29	6.481016246309849
5	30	5.270855024686501
5	31	4.668506132570894
5	32	4.795105743075997
5	33	4.757027907916189
5	34	4.566443708730116
5	35	5.104004276633077
5	36	7.084258969163201
5	37	7.232689365464058
5	38	6.798710232510096
5	39	7.662351430010961
5	40	8.70756840922645
5	41	12.007304549602399
5	42	10.878186275481898
5	43	11.359576914986615
5	44	12.33067277584728
5	45	11.141699252363708
5	46	12.876146001107301
5	47	13.226933233063232
5	48	13.00945941978964
5	49	13.338412373707978
5	50	12.837731441856473
5	51	12.455482667180178
5	52	11.830868295452738
5	53	12.070018581943039
5	54	11.890787632259137
5	55	12.282669958825508
5	56	11.032436470664866
6	1	4.839815929262464
6	2	6.165591962269168
6	3	6.196333422136938
6	4	6.609784685558694
6	5	6.6837708111166485
6	6	6.630645754770068
6	7	5.465929090911828
6	8	5.744959509330101
6	9	5.7952758077319615
6	10	6.484484493616964
6	11	5.053315049408575
6	12	6.660200028712595
6	13	6.57949840784838
6	14	5.9123994618827425
6	15	6.80434556305288
6	16	7.174511121313035
6	17	8.225423529778741
6	18	9.477558776764678
6	19	7.462846314089489
6	20	6.954965951801061
6	21	7.389818556175875
6	22	8.035978737781946
6	23	7.495930462821303
6	24	7.960190424262851
6	25	7.643522726341569
6	26	6.234692859927957
6	27	5.572555588313822
6	28	5.857823135026191
6	29	6.212044579973416
6	30	6.389836597898313
6	31	5.0447692591807645
6	32	5.59033062914693
6	33	5.263665514415102
6	34	5.015720506827993
6	35	5.91062206938124
6	36	7.07524371257513
6	37	7.059616252723817
6	38	8.239467117788479
6	39	8.377132488065246
6	40	5.086308254679192
6	41	12.493137717821146
6	42	11.268021821350732
6	43	12.076636554376346
6	44	12.981653556509055
6	45	11.903537968432659
6	46	13.195345525577288
6	47	14.163628299439292
6	48	13.002722744537014
6	49	14.459771597008949
6	50	13.46840312800666
6	51	12.439829804093193
6	52	12.333047808034472
6	53	12.498788148021895
6	54	11.901487057660365
6	55	12.45069056512628
6	56	11.294572392994962
7	1	5.025697806285123
7	2	6.1120927713306585
7	3	6.194007370356999
7	4	6.778372873260963
7	5	7.0867845651619525
7	6	6.566426499106637
7	7	5.306139446898882
7	8	5.594777470495153
7	9	5.853427102230349
7	10	6.3241891861736015
7	11	5.35165647335679
7	12	6.154030934861467
7	13	6.770234653803076
7	14	5.939503021753289
7	15	7.538265465965505
7	16	8.07894073296352
7	17	9.106491490943792
7	18	9.246470589062408
7	19	8.65166103900858
7	20	7.9967337511538155
7	21	7.7491429898328335
7	22	8.173519190856375
7	23	7.9402063527889375
7	24	7.710190424262851
7	25	7.9216376130729635
7	26	6.9040901091188935
7	27	6.361592805142305
7	28	6.179930740204188
7	29	6.3881165864459035
7	30	7.082292099516433
7	31	5.302859874067497
7	32	6.230905062803888
7	33	6.166679268460406
7	34	6.102795749546441
7	35	5.816164140578657
7	36	7.683961350115581
7	37	7.873633243015082
7	38	8.476218735911452
7	39	9.688924559262663
7	40	9.517942558886315
7	41	12.780051148241856
7	42	11.554631853713193
7	43	12.066422153081849
7	44	12.538793524146595
7	45	12.41891013671745
7	46	13.1933228718556
7	47	14.252018267076831
7	48	13.476933909585558
7	49	14.223829040374643
7	50	12.460818176550351
7	51	13.166367020921676
7	52	13.679932921303077
7	53	13.598504976500863
7	54	13.494529128857778
7	55	14.125144448621427
7	56	12.803269803998198
8	1	4.404864472951786
8	2	5.875009437997321
8	3	6.001090703690338
8	4	6.326289539927629
8	5	6.86386789849529
8	6	6.448509832439971
8	7	5.454472780232216
8	8	5.226027470495154
8	9	5.736343768897015
8	10	6.627105852840263
8	11	5.207489806690127
8	12	6.331947601528128
8	13	6.729401320469737
8	14	6.3178363550866266
8	15	6.712015465965504
8	16	7.895190732963517
8	17	9.506491490943791
8	18	9.421053922395746
8	19	8.036244372341919
8	20	8.13215041782048
8	21	7.70622632316617
8	22	8.47685252418971
8	23	8.007289686122274
8	24	8.43019042426285
8	25	7.400804279739628
8	26	7.008673442452231
8	27	6.345759471808972
8	28	6.180347406870855
8	29	7.08019991977924
8	30	7.231875432849765
8	31	5.54952654073416
8	32	6.576738396137227
8	33	6.253762601793738
8	34	5.65154574954644
8	35	5.720330807245318
8	36	7.088544683448916
8	37	7.187383243015081
8	38	7.558302069244789
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115	18	5.032928922395744
115	19	3.750619372341916
115	20	3.4290254178204798
115	21	5.988726323166169
115	22	6.756852524189711
115	23	6.414164686122273
115	24	5.366440424262851
115	25	6.135179279739628
115	26	5.753048442452229
115	27	4.840759471808969
115	28	5.221597406870853
115	29	5.158949919779239
115	30	6.431250432849769
115	31	4.094526540734162
115	32	4.423613396137224
115	33	4.812512601793742
115	34	4.540295749546441
115	35	5.107830807245321
115	36	6.923544683448917
115	37	6.608008243015082
115	38	6.903302069244788
115	39	7.902466225929327
115	40	9.200234225552979
115	41	10.71234281490852
115	42	8.799423520379857
115	43	7.774755486415184
115	44	0.9033768574799304
115	45	8.101201803384114
115	46	3.729781205188935
115	47	-5.883398399589833
115	48	3.305058909585558
115	49	-1.2240876262920217
115	50	4.148318176550351
115	51	4.59865868758834
115	52	4.200766254636413
115	53	5.124546643167527
115	54	5.067445795524442
115	55	4.953269448621427
115	56	7.173061470664866
116	1	4.390578758666074
116	2	5.270723723711612
116	3	5.599662132261763
116	4	6.083432397070482
116	5	6.428153612780999
116	6	5.939938403868542
116	7	5.28590135166079
116	8	5.0474560419237235
116	9	4.612058054611296
116	10	5.068534424268837
116	11	3.0189183781186983
116	12	1.7590904586709897
116	13	3.0208298918983125
116	14	2.4121220693723373
116	15	-4.603698819748779
116	16	2.3137621615349495
116	17	4.58506291951522
116	18	4.603911065252884
116	19	4.3876729437704896
116	20	3.0250075606776257
116	21	4.547654894594743
116	22	5.906852524189709
116	23	4.774432543265132
116	24	5.945904709977139
116	25	5.3293757083110584
116	26	4.825816299595086
116	27	4.408616614666112
116	28	3.6974902640137124
116	29	4.597342776922098
116	30	4.021875432849772
116	31	3.0052408264484463
116	32	3.0053098247086503
116	33	3.308048316079457
116	34	2.807260035260729
116	35	2.791759378673895
116	36	5.269973254877485
116	37	5.067383243015083
116	38	6.0068734978162155
116	39	6.843984083072183
116	40	7.609609225552976
116	41	7.558324957765663
116	42	5.765941377522715
116	43	1.38814834355804
116	44	3.4408768574799318
116	45	5.164148231955544
116	46	-1.0197723662396356
116	47	-5.93429125673269
116	48	-1.3436018047001568
116	49	-1.990159054863451
116	50	-1.013288966306792
116	51	2.433033687588342
116	52	2.5471948260649846
116	53	2.416957357453242
116	54	1.9969100812387275
116	55	2.2438944486214254
116	56	5.218150756379153
117	1	3.844150187237503
117	2	4.506438009425892
117	3	4.424662132261766
117	4	5.083432397070482
117	5	5.4781536127810035
117	6	5.529224118154257
117	7	5.014472780232218
117	8	4.804598899066583
117	9	4.8549151974684435
117	10	5.289962995697412
117	11	-0.0739387647384433
117	12	-5.737338112757584
117	13	-8.286312965244544
117	14	-2.1914493591990905
117	15	-7.350127391177352
117	16	-7.318380695607907
117	17	0.0172057766580771
117	18	-2.638946077604256
117	19	0.3019586580562006
117	20	-0.5285638678938049
117	21	5.051226323166169
117	22	6.32828109561828
117	23	5.474432543265127
117	24	6.156618995691421
117	25	5.7043757083110584
117	26	6.215102013880799
117	27	5.215759471808969
117	28	5.297490264013707
117	29	6.208057062636385
117	30	6.339732575706915
117	31	4.7373836835913075
117	32	5.448166967565793
117	33	5.486619744650888
117	34	5.767974320975011
117	35	5.884616521531036
117	36	7.887830397734632
117	37	8.163811814443651
117	38	8.278302069244788
117	39	9.386841225929327
117	40	11.720323511267264
117	41	8.79046781490852
117	42	8.515941377522715
117	43	-2.0154230850133885
117	44	0.4837340003370727
117	45	6.728433946241257
117	46	0.00879906233179284
117	47	-1.3878626853041176
117	48	0.8921124810141308
117	49	-0.4651590548634488
117	50	3.0902824622646357
117	51	6.600890830445483
117	52	6.272194826064986
117	53	6.756243071738954
117	54	7.8933386526673
117	55	7.397465877192854
117	56	12.032436470664866
118	1	3.377413492559633
118	2	4.36148002623262
118	3	4.861286782121709
118	4	5.177269932084492
118	5	5.4452404475148946
118	6	5.231254930479185
118	7	4.102708074349863
118	8	4.023086294024566
118	9	4.132226121838187
118	10	4.671615656761833
118	11	-0.5564317619373256
118	12	-9.182366123962066
118	13	-6.280010444236144
118	14	-8.935496978246707
118	15	-8.17680806344626
118	16	-10.671672012134518
118	17	-1.104292822781698
118	18	-6.0703186266238625
118	19	-2.0621870002071034
118	20	-2.1276535037481494
118	21	3.5487753427740145
118	22	5.261754484974027
118	23	3.391799490043841
118	24	4.626857090929516
118	25	2.8517846718964925
118	26	4.533379324805171
118	27	3.34811241298544
118	28	2.6756415245179106
118	29	3.3715724687988455
118	30	2.968934256379182
118	31	2.709722619165536
118	32	3.6512482000587916
118	33	2.8486645625780582
118	34	2.8411535926836997
118	35	2.253664140578657
118	36	4.710309389331272
118	37	4.114442066544495
118	38	4.533204030029104
118	39	5.269194167105798
118	40	6.505687656925527
118	41	6.0821344815751885
118	42	5.124484794889661
118	43	2.4408094079838136
118	44	2.595778818264243
118	45	5.580674842599802
118	46	-1.9365790889287133
118	47	-1.477148399589833
118	48	-2.2626494237477743
118	49	-0.8478621360959444
118	50	-0.6488631959986684
118	51	2.038916040529518
118	52	2.8502760585579807
118	53	1.5850245843439978
118	54	2.5386467759166003
118	55	1.971345429013585
118	56	3.012828627527611
119	1	3.538197806285119
119	2	4.885009437997326
119	3	5.004424037023668
119	4	5.42628953992763
119	5	5.4305345651619525
119	6	5.6185098324399725
119	7	4.864472780232219
119	8	4.342694137161821
119	9	4.459677102230344
119	10	4.713772519506936
119	11	-0.0858435266432096
119	12	-6.131385731805203
119	13	-3.4672653461969247
119	14	-5.518830311580043
119	15	-5.184651200701161
119	16	-5.231475933703145
119	17	0.6064914909437924
119	18	-4.772279410937589
119	19	-0.6337556276580827
119	20	-0.2845162488461881
119	21	3.1595596564995034
119	22	3.9568525241897134
119	23	3.2339563527889403
119	24	2.4435237575961857
119	25	3.6674709464062936
119	26	2.7020067757855593
119	27	3.399092805142299
119	28	3.290347406870854
119	29	3.7401999197792364
119	30	3.9552087661831017
119	31	2.7361932074008273
119	32	2.7100717294705596
119	33	3.710429268460409
119	34	2.2548790828797713
119	35	3.236997473911991
119	36	5.081878016782248
119	37	4.784049909681752
119	38	4.711635402578118
119	39	6.02017455926266
119	40	8.209609225552978
119	41	5.86546781490852
119	42	3.6587985203798574
119	43	-3.752327846918149
119	44	-1.1924564758534046
119	45	5.185576803384116
119	46	-2.4483437948110662
119	47	-1.227148399589833
119	48	-4.829316090414441
119	49	-2.6282542929586867
119	50	-0.27043182344964833
119	51	4.58303368758834
119	52	5.209099587969746
119	53	5.036004976500859
119	54	4.7611957955244435
119	55	5.1438944486214275
119	56	7.065769803998201
120	1	3.8820574554079244
120	2	4.939395402909604
120	3	4.932494212462267
120	4	5.515763224138155
120	5	5.803341582705816
120	6	5.596580007878568
120	7	4.83026225391643
120	8	4.797080102074098
120	9	5.022834996967191
120	10	5.151491817752547
120	11	-0.26128212313443555
120	12	-7.871736608998184
120	13	-3.715510960232013
120	14	-2.9530408378958306
120	15	-0.15307225333274133
120	16	-5.3569145301943735
120	17	0.8214037716455458
120	18	-2.0424548495340815
120	19	-0.4048082592370328
120	20	-0.1266215120040819
120	21	3.4920157968503815
120	22	4.7086069101546215
120	23	3.9593949492801706
120	24	4.433874634789166
120	25	3.9797516481606827
120	26	5.645866424908366
120	27	5.009619120931777
120	28	4.6684175823094485
120	29	5.9577437794283625
120	30	5.163980696007666
120	31	4.166894961786792
120	32	4.218843659295121
120	33	4.2253415491621595
120	34	4.4180369776166195
120	35	4.201032561631287
120	36	5.158193806255937
120	37	4.765628857050171
120	38	5.330933648192158
120	39	6.474560524174944
120	40	6.379784664149469
120	41	-8.77751464123183
120	42	-7.547341830497336
120	43	-8.647941882005869
120	44	-15.010000335502525
120	45	-7.918809161528166
120	46	-9.55887011060054
120	47	-7.0736396276600075
120	48	-7.2275617044495295
120	49	-13.933517450853426
120	50	-7.1142914725724555
120	51	-3.1932821018853446
120	52	-3.582128482205693
120	53	-2.4903108129728224
120	54	-3.1063480641246812
120	55	-2.0420704636592752
120	56	-0.3710723012649595
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2401.0
5	225.0
6	158.0
7	171.0
8	369.0
9	313.0
10	192.0
11	168.0
12	158.0
13	170.0
14	222.0
15	268.0
16	373.0
17	472.0
18	553.0
19	710.0
20	892.0
21	1082.0
22	1274.0
23	1471.0
24	1652.0
25	1798.0
26	1883.0
27	1966.0
28	2105.0
29	2306.0
30	2605.0
31	2993.0
32	3400.0
33	3748.0
34	4072.0
35	4471.0
36	4812.0
37	5289.0
38	4624.0
39	3283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.4221775287714	0.49641654296157955	0.05267442417277211	0.02873150409423933
8	99.53550735047646	0.2952960143019043	0.05746300818847866	0.11173362703315295
9	98.7948537862342	0.3447835525475674	0.5634657131911633	0.2968969480270719
10	54.513240434803436	16.598828393110825	16.373764944372617	12.514166227713133
11	37.04767833484972	21.952465322670754	15.553320883014893	25.446535459464638
12	32.13459113473479	22.94370221392201	17.987517757665724	26.934188893677476
13	24.383469807977782	14.633912751999233	21.67153506041597	39.311082379607015
14	21.215023384251943	30.255870005905923	23.35871282861658	25.17039378122556
15	32.523556182296005	18.917056598935964	22.448881481956285	26.110505736811746
16	29.14543973681269	28.283054122682337	22.11539997125381	20.45610616925116
17	26.863145794750622	18.918832620218403	34.14809374800434	20.06992783702663
18	23.97324777729892	19.14475889479481	24.723459273092946	32.15853405481332
19	24.92214325640821	21.913279565599296	20.967819212648724	32.19675796534377
20	25.368269691644034	22.364594983224155	30.492091388400706	21.775043936731105
21	23.962074414595605	21.167137544094878	23.982824945330332	30.887963095979188
22	19.70183083528867	20.302000031923896	34.96304809334546	25.03312103944197
23	20.434484189691776	22.295647177129723	32.38678989289534	24.883078740283164
24	20.700774203847075	22.57003751296991	24.77452310639317	31.95466517678985
25	24.487580614264733	22.768341740629587	29.795351510120682	22.948726134984994
26	33.041631401606494	22.722409415371843	21.08557832037176	23.15038086264991
27	21.759688188875757	29.532602792243058	20.7804862464458	27.927222772435385
28	25.830446789151917	26.899931361437897	22.250067840439286	25.0195540089709
29	30.867612028584674	26.319323432079422	19.799843327844478	23.013221211491423
30	30.875001996262995	21.131641566986602	27.13799766836482	20.85535876838558
31	31.724644390874694	26.004565845559473	22.099650377560305	20.171139386005525
32	32.548374736573216	25.445430742703877	21.97617983268408	20.03001468803883
33	31.972386902954664	24.880550983556784	21.83480081177392	21.31226130171463
34	23.625925511330017	22.26344490109222	34.394639630275215	19.715989957302543
35	23.256706205838157	24.252645714103018	33.889119800492374	18.601528279566455
36	27.596339652171135	24.750467125540986	27.230624271364007	20.422568950923868
37	28.75457582684592	22.891123295554454	28.204677334271146	20.149623543328484
38	30.285385206040438	25.156769900179164	24.59847709239826	19.959367801382136
39	30.187141418822815	24.413085516117174	20.082144055742894	25.317629009317116
40	23.040896142146686	18.875637744837686	24.80163520190559	33.28183091111004
41	20.122600419422486	16.28165833198903	21.687368930472655	41.90837231811583
42	22.41448305668049	27.755990587933987	21.956685288044437	27.872841067341092
43	19.154384111895272	23.552098836558006	27.43130571158801	29.862211339958712
44	21.07396961733222	24.895840010255753	25.573681174283703	28.456509198128327
45	26.381290590907636	22.24461720574591	27.579422209425086	23.79466999392136
46	20.723879044005827	29.017592725992092	23.891850357777457	26.366677872224624
47	17.835446487248944	24.745539936204658	23.270873739721416	34.14813983682498
48	25.04351992334105	22.461071628204106	25.139343607761717	27.356064840693122
49	20.713154484810673	24.394106637231847	23.703028267025005	31.189710610932476
50	21.998177079169132	25.95583414618546	25.153109359259318	26.89287941538609
51	20.839136211560934	26.422705662008354	26.818101778482127	25.920056347948584
52	21.271288078675944	29.856880147117614	26.2189174062525	22.652914367953947
53	21.290353558795754	27.761327806142866	26.25042014116743	24.69789849389395
54	21.85355240213096	22.77504919449022	29.636680691762525	25.7347177116163
55	20.20324877970713	23.0887412979115	29.54148995758982	27.16651996479155
56	20.48894435378068	22.912866788262775	30.453425490384305	26.14476336757224
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	1.5
5	1.0
6	2.5
7	4.0
8	8.0
9	12.0
10	25.5
11	39.0
12	48.0
13	67.0
14	77.0
15	91.0
16	105.0
17	113.5
18	122.0
19	134.0
20	146.0
21	149.0
22	162.0
23	172.0
24	193.0
25	214.0
26	229.0
27	244.0
28	295.0
29	377.0
30	408.0
31	496.5
32	585.0
33	678.0
34	771.0
35	810.0
36	849.0
37	918.5
38	1062.0
39	1136.0
40	1359.5
41	1583.0
42	2159.0
43	2735.0
44	3821.5
45	4908.0
46	5029.0
47	4562.5
48	3975.0
49	3910.5
50	3846.0
51	3792.0
52	3738.0
53	3913.5
54	3860.0
55	3631.0
56	3924.0
57	4217.0
58	4294.0
59	4371.0
60	3981.0
61	3591.0
62	3116.5
63	2494.5
64	2347.0
65	1868.0
66	1389.0
67	1340.5
68	1292.0
69	1091.0
70	890.0
71	843.5
72	572.0
73	347.0
74	262.0
75	177.0
76	152.5
77	128.0
78	193.0
79	179.5
80	101.0
81	58.0
82	15.0
83	8.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.001596194671902185
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.3910676946160354
16	0.05107822950086992
17	0.020750530734728406
18	0.0
19	0.05427061884467429
20	0.09417548564222893
21	0.0
22	0.0
23	0.0
24	0.00638477868760874
25	0.007980973359510925
26	0.04469345081326118
27	0.0750211495794027
28	0.00319238934380437
29	0.15642707784641413
30	0.05107822950086992
31	0.01596194671902185
32	0.020750530734728406
33	0.11173362703315295
34	0.1851585819406535
35	0.1516384938307076
36	0.05107822950086992
37	0.14684990981500104
38	0.21867867005059935
39	0.12131079506456606
40	0.8236364507015275
41	1.05189228878354
42	0.28093026225478457
43	0.258583536848154
44	0.3910676946160354
45	0.21548628070679499
46	0.2873150409423933
47	0.41820300403837246
48	0.05427061884467429
49	0.22027486472250155
50	0.17877380325304473
51	0.2873150409423933
52	0.1819661925968491
53	0.27135309422337145
54	0.22665964341011027
55	0.26177592619195833
56	0.2346406167696212
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	62649.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.43528228702772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1268384435833	62.77674025124104
2	6.3883789969859714	8.99934556018452
3	1.717769166270084	3.6297466839055694
4	0.7682371337276497	2.164439975099363
5	0.4441725020962223	1.5642707784641416
6	0.28327327939810093	1.1971460039266388
7	0.22208625104811114	1.094989544924899
8	0.16996396763886057	0.957716803141311
9	0.1201078704647948	0.7613848584973424
>10	0.670791125614703	8.739165828664463
>50	0.05438846964443538	2.5826429791377357
>100	0.029460421057402496	3.4397995179492087
>500	0.004532372470369615	2.092611214863765
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATCAACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	764	1.2194927293332694	No Hit
ATCAACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	547	0.8731184855304953	No Hit
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	303	0.4836469855863621	No Hit
ATCAACGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	241	0.3846829159284266	No Hit
ATCAACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	229	0.3655285798656004	No Hit
ATCAACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	186	0.2968922089738064	No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	180	0.2873150409423933	No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	176	0.28093026225478457	No Hit
ATCAACGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	138	0.22027486472250155	No Hit
ATCAACGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	130	0.20750530734728406	No Hit
ATCAACGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	124	0.197928139315871	No Hit
ATCAACGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	119	0.18994716595636005	No Hit
ATCAACGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	115	0.1835623872687513	No Hit
ATCAACGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	109	0.17398521923733817	No Hit
ATCAACGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	105	0.16760044054972945	No Hit
ATCAACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	100	0.15961946719021852	No Hit
ATCAACGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC	95	0.1516384938307076	No Hit
ATCAACGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT	93	0.1484461044869032	No Hit
ATCAACGGGGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA	84	0.13408035243978356	No Hit
ATCAACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	83	0.13248415776788136	No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	83	0.13248415776788136	No Hit
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	80	0.1276955737521748	No Hit
ATCAACGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA	79	0.12609937908027263	No Hit
ATCAACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	68	0.10854123768934858	No Hit
ATCAACGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG	64	0.10215645900173984	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0031923893438043703	0.0	0.0	0.0	0.0
11	0.004788584015706555	0.0	0.0	0.0	0.0
12	0.004788584015706555	0.0	0.0	0.0	0.0
13	0.006384778687608741	0.0	0.0	0.0	0.0
14	0.027135309422337148	0.0	0.0	0.0	0.0
15	0.03032769876614152	0.0	0.0	0.0	0.0
16	0.03671247745375026	0.0	0.0	0.0	0.0
17	0.03830867212565244	0.0	0.0	0.0	0.0
18	0.03830867212565244	0.0	0.0	0.0	0.0
19	0.043097256141359	0.0	0.0	0.0	0.0
20	0.04469345081326118	0.0	0.0	0.0	0.0
21	0.047885840157065554	0.0	0.0	0.0	0.0
22	0.051078229500869925	0.0	0.0	0.0	0.0
23	0.052674424172772114	0.0	0.0	0.0	0.0
24	0.052674424172772114	0.0	0.0	0.0	0.0
25	0.055866813516576484	0.0	0.0	0.0	0.0
26	0.055866813516576484	0.0	0.0	0.0	0.0
27	0.057463008188478666	0.0	0.0	0.0	0.0
28	0.0654439815479896	0.0	0.0	0.0	0.0
29	0.06704017621989178	0.0	0.0	0.0	0.0
30	0.07023256556369614	0.0	0.0	0.0	0.0
31	0.07182876023559834	0.0	0.0	0.0	0.0
32	0.07182876023559834	0.0	0.0	0.0	0.0
33	0.07821353892320708	0.0	0.0	0.0	0.0
34	0.07980973359510926	0.0	0.0	0.0	0.0
35	0.08300212293891363	0.0	0.0	0.0	0.0
36	0.08459831761081582	0.0	0.0	0.0	0.0
37	0.08459831761081582	0.0	0.0	0.0	0.0
38	0.08459831761081582	0.0	0.0	0.0015961946719021851	0.0
39	0.08779070695462018	0.0	0.0	0.0015961946719021851	0.0
40	0.09098309629842456	0.0	0.0	0.0015961946719021851	0.0
41	0.09417548564222893	0.0	0.0	0.0015961946719021851	0.0
42	0.10375265367364203	0.0	0.0	0.0015961946719021851	0.0
43	0.10694504301744641	0.0	0.0	0.0015961946719021851	0.0
44	0.11173362703315297	0.0	0.0	0.0015961946719021851	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGAA	15	0.007846355	50.85948	41
TGTCGAC	15	0.007846355	50.85948	41
CGCATTG	35	4.587855E-8	50.776512	39
TTCACTC	15	0.00789722	50.776512	40
GCGCATT	35	4.587855E-8	50.776512	38
GCGGATC	30	9.275627E-7	50.776512	38
GCGGATA	15	0.00789722	50.776512	38
GCACTAA	15	0.00789722	50.776512	40
AAGAGAC	15	0.00794833	50.693813	35
TAAGACC	15	0.00794833	50.693813	35
GGCGCAT	35	4.647336E-8	50.69381	37
TGACCGA	15	0.008025455	50.57027	34
ACTTGGC	20	3.926865E-4	50.570267	34
TAAGAGA	20	3.926865E-4	50.570267	34
TCCGCCA	15	0.008286566	50.162773	45
GTGGTAC	20	4.08706E-4	50.16277	45
TGGTACA	20	4.08706E-4	50.16277	46
AGCGTGG	15	0.00831302	50.122383	42
CACTCCG	15	0.00831302	50.122383	42
TCACATA	15	0.008339536	50.08206	10
>>END_MODULE