FastQCFastQC Report
Fri 17 Jun 2016
SRR1525809_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525809_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2626
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC90.3427265803503427No Hit
ATCAACGGGATGCCACAACTAGATACATCAACATGATTTA70.26656511805026656No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC70.26656511805026656No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG60.2284843869002285No Hit
ATCAACGGGCAGCGACTTTTTTTTTTTTTTTTTTTTTTTT50.19040365575019041No Hit
ATCAACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT50.19040365575019041No Hit
ATCAACGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC40.15232292460015232No Hit
ATCAACGGGCTACGAAGACCTTTTTTTTTTTTTTTTTTTT40.15232292460015232No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC40.15232292460015232No Hit
ATCAACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCT40.15232292460015232No Hit
ATCAACGGGGGAGACTTGTCGCCTTATGGTTTCCATGTTG40.15232292460015232No Hit
ATCAACGGGATGCCCCAACTAGATACATCAACATGATTTA30.11424219345011424No Hit
ATCAACGGGATCCCAAGGCGTATTTTGAAAGTTTGAATGC30.11424219345011424No Hit
ATCAACGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT30.11424219345011424No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG30.11424219345011424No Hit
ATCAACGGGGGGCTGGAGCGATGGCTCAGCCGTTAAAGGC30.11424219345011424No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGC30.11424219345011424No Hit
ATCAACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT30.11424219345011424No Hit
ATCAACGGGCTCTCTTGCTTCTCTCTCTTGCTTTTTCGCT30.11424219345011424No Hit
ATCAACGGGTATCAACGCAGAGTCTTTTTTTTTTTTTTTT30.11424219345011424No Hit
ATCAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT30.11424219345011424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG355.585589E-734.529416
ACGGGGG700.034.529415
ACGGGGC200.002133108734.529415
ACGGGGA308.867131E-634.529415
ACGGGCT200.002133108734.529415
AACGGGT308.867131E-634.529414
AACGGGC452.1027518E-934.529414
AACGGGA308.867131E-634.529414
TCAACGG2600.033.8653832
CAACGGG2500.033.838823
AACGGGG1450.033.338744
ATCAACG2650.033.2264181
ACGGGGT250.006369650427.623535