##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525809_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2626
Sequences flagged as poor quality	0
Sequence length	40
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.875476009139376	30.0	22.0	33.0	4.0	36.0
2	24.26085300837776	27.0	17.0	33.0	4.0	37.0
3	20.67022086824067	22.0	11.0	31.0	4.0	36.0
4	23.8004569687738	27.0	16.0	33.0	4.0	36.0
5	23.546077684691546	26.0	17.0	33.0	4.0	36.0
6	17.052551408987053	15.0	11.0	23.0	4.0	33.0
7	22.605102817974107	26.0	13.0	32.0	4.0	35.0
8	23.76047220106626	28.0	15.0	33.0	4.0	36.0
9	22.643945163747144	26.0	13.0	32.0	4.0	36.0
10	20.05026656511805	22.0	8.0	30.0	4.0	35.0
11	20.34120335110434	22.0	8.0	31.0	4.0	35.0
12	20.092155369383093	22.0	4.0	31.0	4.0	35.0
13	20.65003808073115	24.0	4.0	31.0	4.0	35.0
14	21.012566641279513	25.0	4.0	32.0	4.0	35.0
15	19.509520182787508	21.0	4.0	31.0	4.0	35.0
16	19.345011424219344	20.0	4.0	32.0	4.0	36.0
17	19.925742574257427	23.0	4.0	32.0	4.0	36.0
18	19.922696115765422	23.0	4.0	32.0	4.0	36.0
19	17.392612338156894	17.0	4.0	30.0	4.0	35.0
20	18.963442498095965	21.0	4.0	32.0	4.0	35.0
21	16.567022086824068	14.0	4.0	30.0	4.0	35.0
22	16.575399847677076	13.0	4.0	30.0	4.0	34.0
23	16.728484386900227	14.0	4.0	30.0	4.0	34.0
24	16.00875856816451	10.0	4.0	30.0	4.0	34.0
25	15.231150038080731	4.0	4.0	29.0	4.0	34.0
26	13.002284843869003	4.0	4.0	25.0	4.0	33.0
27	12.974485910129474	4.0	4.0	26.0	4.0	33.0
28	12.768469154607768	4.0	4.0	25.0	4.0	33.0
29	12.218202589489719	4.0	4.0	23.0	4.0	32.0
30	11.748667174409748	4.0	4.0	22.0	4.0	32.0
31	11.167174409748668	4.0	4.0	19.0	4.0	32.0
32	10.682787509520184	4.0	4.0	17.0	4.0	31.0
33	10.234958111195734	4.0	4.0	14.0	4.0	31.0
34	9.636709824828637	4.0	4.0	10.0	4.0	30.0
35	9.251713632901751	4.0	4.0	4.0	4.0	30.0
36	8.78903274942879	4.0	4.0	4.0	4.0	30.0
37	8.76123381568926	4.0	4.0	4.0	4.0	30.0
38	8.477913175932978	4.0	4.0	4.0	4.0	29.0
39	8.225057121096725	4.0	4.0	4.0	4.0	28.0
40	8.220868240670221	4.0	4.0	4.0	4.0	27.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	7.085798031333741
1	2	-5.877627035662753
1	3	-3.4797026386312098
1	4	5.864013863121013
1	5	8.178360131931559
1	6	3.9262471655328817
1	7	11.794271799628941
1	8	5.913198309626882
1	9	1.344061791383222
1	10	6.641827715934859
1	11	8.72943465264894
1	12	7.594014120799834
1	13	-0.5433029272315011
1	14	11.853621675943103
1	15	9.925521799628939
1	16	9.540535456606882
1	17	3.5644944341372913
1	18	3.7203604926819196
1	19	-0.9924487219130054
1	20	6.423340548340548
1	21	12.453543083900229
1	22	5.488522984951556
1	23	9.919323335394767
1	24	7.841196402803545
1	25	9.822835497835499
1	26	14.040148938363224
1	27	6.2325886415172125
1	28	13.045539579468153
1	29	8.929183415790561
1	30	3.072827767470626
1	31	0.44470727685013145
1	32	-0.4293096784168213
1	33	1.4021748093176676
1	34	-0.48916847041847156
1	35	-0.4219490826633683
1	36	-2.478358843537415
1	37	0.21428313749742234
1	38	0.11520047412904333
1	39	-1.7397585549371257
1	40	-0.45291434755720505
6	1	3.1572266027623144
6	2	0.9080872500515333
6	3	5.52029736136879
6	4	-7.70741470830756
6	5	-5.750211296639868
6	6	8.211961451247166
6	7	8.079986085343226
6	8	9.198912595341167
6	9	8.772633219954649
6	10	-0.5724579983508562
6	11	0.7294346526489406
6	12	3.0940141207998337
6	13	5.813839929911357
6	14	1.567907390228818
6	15	7.996950371057512
6	16	10.254821170892598
6	17	14.778780148423007
6	18	10.791789064110493
6	19	15.078979849515566
6	20	10.99476911976912
6	21	8.882114512471656
6	22	5.774237270665841
6	23	12.205037621109051
6	24	10.55548211708926
6	25	18.537121212121214
6	26	-0.17413677592249144
6	27	4.875445784374355
6	28	-1.0258889919604197
6	29	5.5720405586477035
6	30	-7.212886518243661
6	31	-6.269578437435582
6	32	-6.143595392702535
6	33	-5.883539476396619
6	34	-4.060597041847043
6	35	-4.279091939806225
6	36	-4.335501700680272
6	37	-3.6428597196454344
6	38	-3.7419423830138134
6	39	-3.311187126365697
6	40	-3.5957714904143483
7	1	6.9905599360956465
7	2	7.408087250051533
7	3	12.186964028035458
7	4	13.29258529169244
7	5	13.749788703360132
7	6	13.878628117913834
7	7	4.746652752009894
7	8	4.198912595341167
7	9	5.772633219954649
7	10	13.927542001649144
7	11	14.396101319315605
7	12	10.260680787466502
7	13	8.980506596578024
7	14	14.234574056895482
7	15	19.496950371057512
7	16	9.58815450422593
7	17	15.278780148423007
7	18	8.95845573077716
7	19	0.24564651618223365
7	20	13.661435786435788
7	21	8.215447845804988
7	22	3.1075706039991733
7	23	8.538370954442383
7	24	7.888815450422593
7	25	6.203787878787882
7	26	6.325863224077509
7	27	5.375445784374355
7	28	2.1407776747062464
7	29	10.405373891981036
7	30	-3.2128865182436606
7	31	8.730421562564418
7	32	2.5230712739641312
7	33	9.116460523603381
7	34	1.6060696248196233
7	35	4.720908060193775
7	36	-4.335501700680272
7	37	-3.6428597196454344
7	38	-3.7419423830138134
7	39	-3.311187126365697
7	40	-3.5957714904143483
9	1	-1.3427733972376856
9	2	2.693801535765818
9	3	-1.193988352916925
9	4	1.2925852916924399
9	5	-1.3930684394970108
9	6	-1.2166099773242625
9	7	1.5085575139146528
9	8	-0.22965883323026048
9	9	1.344061791383222
9	10	2.7846848587920014
9	11	2.0151489383632253
9	12	4.736871263656976
9	13	5.028125644197072
9	14	1.9964788188002451
9	15	2.3540932282003695
9	16	5.8262497423211705
9	17	12.278780148423007
9	18	10.006074778396208
9	19	11.436122706658423
9	20	9.851911976911978
9	21	6.1678287981859405
9	22	5.631380127808699
9	23	10.919323335394767
9	24	13.412624974232118
9	25	8.394264069264072
9	26	1.611577509791795
9	27	1.6611600700886413
9	28	-0.9544604205318485
9	29	-0.07081658420943882
9	30	2.2156849103277683
9	31	3.1589929911358468
9	32	4.713547464440323
9	33	-3.3121109049680477
9	34	3.0822601010100996
9	35	2.4351937744794885
9	36	-1.1926445578231286
9	37	4.785711708925994
9	38	-0.5990852401566702
9	39	1.117384302205732
9	40	-0.45291434755720505
10	1	3.557226602762313
10	2	5.908087250051533
10	3	-6.579702638631209
10	4	8.692585291692438
10	5	3.0497887033601323
10	6	6.811961451247168
10	7	6.379986085343226
10	8	10.198912595341167
10	9	6.172633219954648
10	10	10.027542001649145
10	11	7.42943465264894
10	12	11.694014120799835
10	13	9.413839929911358
10	14	10.16790739022882
10	15	7.496950371057512
10	16	11.654821170892596
10	17	11.678780148423005
10	18	12.191789064110491
10	19	12.178979849515567
10	20	9.094769119769122
10	21	10.482114512471657
10	22	13.774237270665841
10	23	11.60503762110905
10	24	8.55548211708926
10	25	8.437121212121212
10	26	-1.4741367759224921
10	27	-1.6245542156256452
10	28	-0.025888991960419716
10	29	-3.6279594413522958
10	30	-1.612886518243661
10	31	-0.7695784374355821
10	32	-0.8435953927025341
10	33	-0.3835394763966189
10	34	-1.260597041847043
10	35	-1.4790919398062252
10	36	-3.235501700680272
10	37	-2.542859719645435
10	38	-2.641942383013814
10	39	-0.8111871263656969
10	40	-2.4957714904143486
12	1	5.657226602762314
12	2	0.40808725005153335
12	3	-5.47970263863121
12	4	-1.7074147083075601
12	5	0.7497887033601316
12	6	0.21196145124716637
12	7	-5.920013914656774
12	8	4.198912595341167
12	9	-2.227366780045351
12	10	1.9275420016491438
12	11	6.729434652648941
12	12	-7.405985879200166
12	13	12.313839929911357
12	14	3.567907390228818
12	15	-7.503049628942488
12	16	11.254821170892598
12	17	2.2787801484230066
12	18	-0.7082109358895075
12	19	9.578979849515566
12	20	10.99476911976912
12	21	16.882114512471656
12	22	6.774237270665841
12	23	18.20503762110905
12	24	18.555482117089262
12	25	17.537121212121214
12	26	-8.674136775922491
12	27	-8.624554215625645
12	28	-8.52588899196042
12	29	-7.9279594413522965
12	30	-7.212886518243661
12	31	-6.269578437435582
12	32	-6.143595392702535
12	33	-5.883539476396619
12	34	-4.060597041847043
12	35	-4.279091939806225
12	36	-4.335501700680272
12	37	-3.6428597196454344
12	38	-3.7419423830138134
12	39	-3.311187126365697
12	40	-3.5957714904143483
13	1	2.6572266027623144
13	2	6.208087250051534
13	3	6.120297361368792
13	4	-5.1074147083075605
13	5	-0.4502112966398677
13	6	3.611961451247165
13	7	7.879986085343226
13	8	7.998912595341167
13	9	9.172633219954648
13	10	7.327542001649142
13	11	11.72943465264894
13	12	13.194014120799835
13	13	15.313839929911357
13	14	13.567907390228818
13	15	11.89695037105751
13	16	15.4548211708926
13	17	17.878780148423008
13	18	14.291789064110493
13	19	16.178979849515567
13	20	9.594769119769122
13	21	8.482114512471657
13	22	11.574237270665842
13	23	6.4050376211090505
13	24	3.3554821170892613
13	25	9.137121212121215
13	26	-0.2741367759224911
13	27	2.775445784374355
13	28	-1.5258889919604197
13	29	2.8720405586477042
13	30	-0.012886518243661271
13	31	3.1304215625644183
13	32	1.8564046072974651
13	33	2.916460523603382
13	34	-0.06059704184704273
13	35	3.5209080601937757
13	36	-0.535501700680272
13	37	1.757140280354566
13	38	-2.5419423830138133
13	39	2.0888128736343035
13	40	-3.5957714904143483
15	1	0.6572266027623144
15	2	12.741420583384869
15	3	15.52029736136879
15	4	5.29258529169244
15	5	12.416455370026796
15	6	17.545294784580502
15	7	17.079986085343226
15	8	13.198912595341167
15	9	14.772633219954649
15	10	17.927542001649144
15	11	12.72943465264894
15	12	15.260680787466498
15	13	12.980506596578024
15	14	13.901240723562154
15	15	9.16361703772418
15	16	6.58815450422593
15	17	8.612113481756339
15	18	9.625122397443825
15	19	0.912313182848898
15	20	10.661435786435788
15	21	15.215447845804988
15	22	12.774237270665841
15	23	16.87170428777572
15	24	18.555482117089262
15	25	14.537121212121214
15	26	8.65919655741084
15	27	9.042112451041023
15	28	10.807444341372912
15	29	2.4053738919810375
15	30	3.1204468150896734
15	31	0.06375489589775185
15	32	2.189737940630799
15	33	-2.883539476396619
15	34	4.272736291486291
15	35	4.387574726860441
15	36	3.664498299319728
15	37	4.357140280354566
15	38	4.924724283652853
15	39	-3.311187126365697
15	40	-3.5957714904143483
16	1	-1.9142019686662586
16	2	1.1223729643372486
16	3	-0.19398835291692507
16	4	-0.5645575654504178
16	5	1.1783601319315586
16	6	-1.5023242630385472
16	7	-0.9200139146567743
16	8	0.6274840239125936
16	9	-0.9416524943310662
16	10	5.7846848587920014
16	11	-3.8419939187796306
16	12	3.4511569779426914
16	13	0.4566970727684989
16	14	2.2821931045145334
16	15	5.3540932282003695
16	16	1.2548211708925976
16	17	6.707351576994434
16	18	7.006074778396208
16	19	2.1504084209441388
16	20	6.423340548340548
16	21	3.310685941043083
16	22	0.3456658420944123
16	23	4.633609049680478
16	24	1.269767831374974
16	25	-0.7485930735930726
16	26	1.611577509791795
16	27	-4.910268501339931
16	28	-8.52588899196042
16	29	-7.9279594413522965
16	30	-7.212886518243661
16	31	-6.269578437435582
16	32	-6.143595392702535
16	33	-5.883539476396619
16	34	-4.060597041847043
16	35	-4.279091939806225
16	36	-4.335501700680272
16	37	-3.6428597196454344
16	38	-3.7419423830138134
16	39	-3.311187126365697
16	40	-3.5957714904143483
17	1	13.032226602762314
17	2	8.283087250051533
17	3	11.27029736136879
17	4	14.41758529169244
17	5	10.499788703360132
17	6	9.461961451247166
17	7	8.329986085343226
17	8	7.198912595341167
17	9	7.897633219954649
17	10	3.802542001649144
17	11	1.2294346526489406
17	12	0.8440141207998337
17	13	1.5638399299113566
17	14	0.9429073902288181
17	15	2.121950371057512
17	16	4.504821170892598
17	17	2.7787801484230066
17	18	2.1667890641104925
17	19	6.953979849515566
17	20	-2.5052308802308794
17	21	0.8821145124716558
17	22	3.274237270665841
17	23	4.455037621109051
17	24	5.1804821170892605
17	25	1.0371212121212139
17	26	-0.42413677592249144
17	27	3.375445784374355
17	28	2.7241110080395803
17	29	-2.8029594413522965
17	30	-1.4628865182436606
17	31	-4.519578437435582
17	32	-3.768595392702535
17	33	-3.633539476396619
17	34	-2.6855970418470427
17	35	-2.029091939806225
17	36	-2.960501700680272
17	37	-1.1428597196454344
17	38	-1.7419423830138134
17	39	-0.1861871263656969
17	40	-1.5957714904143483
18	1	9.929953875489588
18	2	10.498996340960627
18	3	10.974842815914243
18	4	13.838039837146987
18	5	9.477061430632858
18	6	5.211961451247166
18	7	11.625440630797772
18	8	9.38073077715935
18	9	9.772633219954649
18	10	10.836632910740054
18	11	7.002161925376214
18	12	7.684923211708924
18	13	6.67747629354772
18	14	4.931543753865181
18	15	7.042404916512059
18	16	1.7093666254380508
18	17	6.096961966604823
18	18	5.3826981550195825
18	19	3.1244343949701125
18	20	4.267496392496394
18	21	1.8821145124716558
18	22	9.228782725211294
18	23	6.841401257472688
18	24	7.100936662543807
18	25	5.718939393939397
18	26	1.598590496804782
18	27	2.0118094207379915
18	28	4.655929189857764
18	29	-1.4734139868068414
18	30	3.605295299938158
18	31	3.0940579262007812
18	32	3.1291318800247385
18	33	2.752824159967018
18	34	4.121221139971139
18	35	2.9027262420119566
18	36	1.6644982993197281
18	37	1.9025948258091105
18	38	4.62169398062255
18	39	2.779721964543395
18	40	4.31331941867656
19	1	-0.09277339723768563
19	2	11.658087250051533
19	3	4.27029736136879
19	4	10.04258529169244
19	5	7.499788703360132
19	6	10.461961451247166
19	7	1.5799860853432257
19	8	4.948912595341167
19	9	1.272633219954649
19	10	15.177542001649144
19	11	13.97943465264894
19	12	15.594014120799834
19	13	11.063839929911357
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106	2	-2.0919127499484667
106	3	0.020297361368790234
106	4	-11.70741470830756
106	5	-1.7502112966398684
106	6	-5.288038548752834
106	7	-13.420013914656774
106	8	-10.801087404658833
106	9	-4.727366780045351
106	10	-3.572457998350856
106	11	-0.7705653473510594
106	12	-6.905985879200166
106	13	-11.686160070088643
106	14	-1.932092609771182
106	15	-13.503049628942488
106	16	-13.745178829107402
106	17	-13.721219851576993
106	18	-13.708210935889507
106	19	-12.421020150484434
106	20	-14.00523088023088
106	21	-12.117885487528344
106	22	-11.225762729334159
106	23	-10.794962378890949
106	24	-10.44451788291074
106	25	-10.462878787878786
106	26	-8.674136775922491
106	27	-8.624554215625645
106	28	-8.52588899196042
106	29	-7.9279594413522965
106	30	-7.212886518243661
106	31	-6.269578437435582
106	32	-6.143595392702535
106	33	-5.883539476396619
106	34	-4.060597041847043
106	35	-4.279091939806225
106	36	-4.335501700680272
106	37	-3.6428597196454344
106	38	-3.7419423830138134
106	39	-3.311187126365697
106	40	-3.5957714904143483
107	1	-11.842773397237686
107	2	-6.841912749948467
107	3	-7.72970263863121
107	4	-10.70741470830756
107	5	-9.250211296639868
107	6	-6.288038548752834
107	7	-13.420013914656774
107	8	-13.051087404658833
107	9	-10.727366780045351
107	10	-6.822457998350856
107	11	-0.7705653473510594
107	12	-5.405985879200166
107	13	-11.436160070088643
107	14	-3.182092609771182
107	15	-1.2530496289424882
107	16	-0.9951788291074024
107	17	-2.4712198515769934
107	18	2.0417890641104925
107	19	3.328979849515566
107	20	1.7447691197691206
107	21	-6.117885487528344
107	22	-9.475762729334159
107	23	-6.044962378890949
107	24	-4.4445178829107395
107	25	-3.962878787878786
107	26	-1.6741367759224914
107	27	-1.6245542156256452
107	28	-1.7758889919604197
107	29	-3.1779594413522965
107	30	-2.2128865182436606
107	31	1.480421562564418
107	32	0.8564046072974651
107	33	1.866460523603381
107	34	0.9394029581529573
107	35	3.470908060193775
107	36	3.164498299319728
107	37	-0.3928597196454344
107	38	-0.49194238301381343
107	39	-0.0611871263656969
107	40	-0.3457714904143483
108	1	1.6572266027623144
108	2	7.408087250051533
108	3	-2.4797026386312098
108	4	-4.70741470830756
108	5	3.7497887033601316
108	6	-3.7880385487528336
108	7	0.07998608534322571
108	8	4.198912595341167
108	9	4.772633219954649
108	10	10.927542001649144
108	11	15.72943465264894
108	12	6.594014120799834
108	13	8.313839929911357
108	14	13.567907390228818
108	15	13.496950371057512
108	16	8.254821170892598
108	17	13.278780148423007
108	18	14.291789064110493
108	19	-6.421020150484434
108	20	12.99476911976912
108	21	-12.117885487528344
108	22	-11.225762729334159
108	23	-10.794962378890949
108	24	-10.44451788291074
108	25	-10.462878787878786
108	26	-8.674136775922491
108	27	-8.624554215625645
108	28	-8.52588899196042
108	29	-7.9279594413522965
108	30	-7.212886518243661
108	31	-6.269578437435582
108	32	-6.143595392702535
108	33	-5.883539476396619
108	34	-4.060597041847043
108	35	-4.279091939806225
108	36	-4.335501700680272
108	37	-3.6428597196454344
108	38	-3.7419423830138134
108	39	-3.311187126365697
108	40	-3.5957714904143483
109	1	2.6572266027623144
109	2	4.408087250051533
109	3	0.5202973613687902
109	4	1.2925852916924399
109	5	-4.250211296639868
109	6	-3.7880385487528336
109	7	-2.9200139146567743
109	8	2.1989125953411666
109	9	1.772633219954649
109	10	10.927542001649144
109	11	-0.2705653473510594
109	12	13.594014120799834
109	13	3.3138399299113566
109	14	7.567907390228818
109	15	4.496950371057512
109	16	11.254821170892598
109	17	-0.7212198515769934
109	18	15.291789064110493
109	19	13.578979849515566
109	20	13.99476911976912
109	21	16.882114512471656
109	22	17.774237270665843
109	23	6.205037621109051
109	24	18.555482117089262
109	25	18.537121212121214
109	26	22.32586322407751
109	27	14.375445784374355
109	28	22.47411100803958
109	29	18.072040558647704
109	30	23.78711348175634
109	31	12.730421562564418
109	32	22.856404607297463
109	33	23.11646052360338
109	34	-4.060597041847043
109	35	-4.279091939806225
109	36	-4.335501700680272
109	37	-3.6428597196454344
109	38	-3.7419423830138134
109	39	-3.311187126365697
109	40	-3.5957714904143483
112	1	-10.842773397237686
112	2	-14.091912749948467
112	3	-12.47970263863121
112	4	-13.70741470830756
112	5	-7.250211296639868
112	6	-6.288038548752834
112	7	-12.420013914656774
112	8	-8.301087404658833
112	9	-11.227366780045351
112	10	-4.572457998350856
112	11	-12.27056534735106
112	12	-3.9059858792001663
112	13	-7.186160070088643
112	14	-13.932092609771182
112	15	-12.503049628942488
112	16	-0.2451788291074024
112	17	-9.221219851576993
112	18	-2.7082109358895075
112	19	1.5789798495155658
112	20	-10.50523088023088
112	21	-4.617885487528344
112	22	-8.225762729334159
112	23	-2.794962378890949
112	24	-7.9445178829107395
112	25	-2.462878787878786
112	26	4.825863224077509
112	27	4.875445784374355
112	28	2.9741110080395803
112	29	2.5720405586477035
112	30	1.7871134817563394
112	31	7.730421562564418
112	32	7.856404607297465
112	33	8.116460523603381
112	34	-4.060597041847043
112	35	-4.279091939806225
112	36	-4.335501700680272
112	37	-3.6428597196454344
112	38	-3.7419423830138134
112	39	-3.311187126365697
112	40	-3.5957714904143483
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	413.0
5	26.0
6	52.0
7	42.0
8	36.0
9	46.0
10	54.0
11	81.0
12	81.0
13	93.0
14	102.0
15	117.0
16	114.0
17	145.0
18	139.0
19	101.0
20	137.0
21	124.0
22	117.0
23	129.0
24	95.0
25	95.0
26	81.0
27	72.0
28	51.0
29	35.0
30	27.0
31	6.0
32	5.0
33	7.0
34	0.0
35	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.3518871521159	0.49561570720548986	0.15249714067861228	0.0
8	97.82774390243902	0.6097560975609756	0.26676829268292684	1.295731707317073
9	97.18095238095238	1.2952380952380953	0.8761904761904762	0.6476190476190476
10	42.65041888804265	32.33054074638233	11.843107387661842	13.175932977913178
11	33.3968012185834	27.53236862147753	21.62985529322163	17.44097486671744
12	37.75238095238095	15.847619047619046	21.44761904761905	24.952380952380953
13	26.00913937547601	18.164508758568164	25.894897182025893	29.931454683929932
14	20.792079207920793	23.57197258187357	31.454683929931456	24.18126428027418
15	28.865194211728866	21.934501142421933	28.63670982482864	20.563594821020565
16	31.302361005331303	19.383092155369383	24.828636709824828	24.485910129474487
17	27.57044935262757	24.56207159177456	25.361766945925364	22.505712109672505
18	29.055597867479054	24.18126428027418	23.076923076923077	23.686214775323684
19	22.404580152671755	19.198473282442748	19.541984732824428	38.85496183206107
20	27.798933739527797	24.105102817974107	24.752475247524753	23.343488194973343
21	21.210967250571212	22.81035795887281	28.33206397562833	27.646610814927648
22	21.667936024371667	18.43107387661843	29.62680883472963	30.274181264280276
23	26.923076923076923	21.134805788271134	29.55064737242955	22.39146991622239
24	25.333333333333336	18.438095238095237	28.952380952380953	27.276190476190475
25	22.179878048780488	20.579268292682926	28.810975609756095	28.429878048780488
26	16.252390057361378	18.66156787762906	34.7227533460803	30.363288718929255
27	17.104761904761904	16.076190476190476	33.98095238095238	32.838095238095235
28	15.733333333333333	20.152380952380952	33.79047619047619	30.323809523809526
29	18.78095238095238	17.79047619047619	34.36190476190476	29.06666666666667
30	17.20061022120519	15.598779557589626	37.986270022883296	29.214340198321892
31	20.405043943446692	17.80664883454337	39.43446694688575	22.35384027512419
32	22.742857142857144	12.838095238095237	37.94285714285714	26.476190476190474
33	18.545316070068544	14.242193450114243	42.68849961919269	24.523990860624522
34	15.232292460015232	15.460776846915461	47.06778370144707	22.23914699162224
35	13.556740289413558	20.563594821020565	43.183549124143184	22.696115765422697
36	12.871287128712872	21.667936024371667	41.660319878141664	23.8004569687738
37	15.276190476190477	17.18095238095238	41.523809523809526	26.01904761904762
38	15.460776846915461	19.04036557501904	40.860624523990865	24.638233054074636
39	17.707539984767706	18.46915460776847	39.18507235338918	24.638233054074636
40	13.175932977913178	18.354912414318356	41.2033511043412	27.265803503427264
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.0
15	4.0
16	7.0
17	10.0
18	10.0
19	12.0
20	14.0
21	25.5
22	37.0
23	37.0
24	43.5
25	50.0
26	58.0
27	66.0
28	66.0
29	68.5
30	71.0
31	85.5
32	100.0
33	100.0
34	102.0
35	104.0
36	121.0
37	138.0
38	138.0
39	140.5
40	143.0
41	155.0
42	167.0
43	167.0
44	152.0
45	137.0
46	142.0
47	147.0
48	147.0
49	145.0
50	143.0
51	155.5
52	168.0
53	168.0
54	165.0
55	162.0
56	176.0
57	190.0
58	190.0
59	200.5
60	211.0
61	209.0
62	207.0
63	207.0
64	176.5
65	146.0
66	113.5
67	81.0
68	81.0
69	70.0
70	59.0
71	45.5
72	32.0
73	32.0
74	28.0
75	24.0
76	15.0
77	6.0
78	6.0
79	6.0
80	6.0
81	4.5
82	3.0
83	3.0
84	1.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.11424219345011424
8	0.07616146230007616
9	0.03808073115003808
10	0.0
11	0.0
12	0.03808073115003808
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.2284843869002285
20	0.0
21	0.0
22	0.0
23	0.0
24	0.03808073115003808
25	0.07616146230007616
26	0.41888804265041885
27	0.03808073115003808
28	0.03808073115003808
29	0.03808073115003808
30	0.15232292460015232
31	0.3427265803503427
32	0.03808073115003808
33	0.0
34	0.0
35	0.0
36	0.0
37	0.03808073115003808
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	2626.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.5064737242955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.16905901116426	92.8027418126428
2	1.9936204146730463	3.808073115003808
3	0.3987240829346092	1.1424219345011426
4	0.1993620414673046	0.7616146230007617
5	0.07974481658692185	0.38080731150038083
6	0.03987240829346093	0.2284843869002285
7	0.07974481658692185	0.5331302361005331
8	0.0	0.0
9	0.03987240829346093	0.3427265803503427
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	9	0.3427265803503427	No Hit
ATCAACGGGATGCCACAACTAGATACATCAACATGATTTA	7	0.26656511805026656	No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC	7	0.26656511805026656	No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	6	0.2284843869002285	No Hit
ATCAACGGGCAGCGACTTTTTTTTTTTTTTTTTTTTTTTT	5	0.19040365575019041	No Hit
ATCAACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	5	0.19040365575019041	No Hit
ATCAACGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC	4	0.15232292460015232	No Hit
ATCAACGGGCTACGAAGACCTTTTTTTTTTTTTTTTTTTT	4	0.15232292460015232	No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC	4	0.15232292460015232	No Hit
ATCAACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCT	4	0.15232292460015232	No Hit
ATCAACGGGGGAGACTTGTCGCCTTATGGTTTCCATGTTG	4	0.15232292460015232	No Hit
ATCAACGGGATGCCCCAACTAGATACATCAACATGATTTA	3	0.11424219345011424	No Hit
ATCAACGGGATCCCAAGGCGTATTTTGAAAGTTTGAATGC	3	0.11424219345011424	No Hit
ATCAACGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT	3	0.11424219345011424	No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG	3	0.11424219345011424	No Hit
ATCAACGGGGGGCTGGAGCGATGGCTCAGCCGTTAAAGGC	3	0.11424219345011424	No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGC	3	0.11424219345011424	No Hit
ATCAACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.11424219345011424	No Hit
ATCAACGGGCTCTCTTGCTTCTCTCTCTTGCTTTTTCGCT	3	0.11424219345011424	No Hit
ATCAACGGGTATCAACGCAGAGTCTTTTTTTTTTTTTTTT	3	0.11424219345011424	No Hit
ATCAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	3	0.11424219345011424	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.03808073115003808	0.0	0.0	0.0	0.0
24	0.03808073115003808	0.0	0.0	0.0	0.0
25	0.03808073115003808	0.0	0.0	0.0	0.0
26	0.03808073115003808	0.0	0.0	0.0	0.0
27	0.03808073115003808	0.0	0.0	0.0	0.0
28	0.03808073115003808	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGGGG	35	5.585589E-7	34.52941	6
ACGGGGG	70	0.0	34.52941	5
ACGGGGC	20	0.0021331087	34.52941	5
ACGGGGA	30	8.867131E-6	34.52941	5
ACGGGCT	20	0.0021331087	34.52941	5
AACGGGT	30	8.867131E-6	34.52941	4
AACGGGC	45	2.1027518E-9	34.52941	4
AACGGGA	30	8.867131E-6	34.52941	4
TCAACGG	260	0.0	33.865383	2
CAACGGG	250	0.0	33.83882	3
AACGGGG	145	0.0	33.33874	4
ATCAACG	265	0.0	33.226418	1
ACGGGGT	25	0.0063696504	27.62353	5
>>END_MODULE