FastQCFastQC Report
Fri 17 Jun 2016
SRR1525808_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525808_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135
Sequences flagged as poor quality0
Sequence length40
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT21.4814814814814816No Hit
ATCAACTTATGTCTTCCCTGCTTATCTGACGGGCTTGTGG10.7407407407407408No Hit
ATCAACGGGGATCTGCTCGTTGTCTAATGCCCCAGCTCTT10.7407407407407408No Hit
ATCAACATCCCACACCCCAAAAACCCGCAAAAAACAACAC10.7407407407407408No Hit
ATCAACGGGCCCAACACAAAAAAAAAAAAAACAAAACAAA10.7407407407407408No Hit
ATCAACGGGACCAGGCCCCGCCCCCTTTTTTTTTTTTTTT10.7407407407407408No Hit
ATCAACGGGACGCAGTGGTATCAACCCCGAGCCGACTTTT10.7407407407407408No Hit
ATCAACCCGAACCCTGGTCCCCCCGCCCTCCTGTTTCTCG10.7407407407407408No Hit
ATCAACGGGTACATCTTCCAATCCCCATCTTTTCCCATCT10.7407407407407408No Hit
ATCAACTCTCCCCCCCCACCTCCAGCCGCACTAAGAGGCA10.7407407407407408No Hit
ATCAACGGGAGTGGTACCCCCCCCGTCCCCTTTTTTTTTT10.7407407407407408No Hit
ATCAACCCACCAACCCCACACACACCCCGCACCTACACAC10.7407407407407408No Hit
ATCAACCAACACCAAATAAACAAACCACACACACCAAAAA10.7407407407407408No Hit
ATCAACCAACACACCAACCACCAAACACCTTCTCAATAAT10.7407407407407408No Hit
ATCAACGGGAAGCAGCGGCCTCCCCCCCGACTCCAGCTCT10.7407407407407408No Hit
ATCAACGAACAGTCGAATCTTTTAACCCCCCCCCCCACCC10.7407407407407408No Hit
ATCAACCGGCCCGAATACCCCCCCCCCCCACCCCCCCCCC10.7407407407407408No Hit
ATCAACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10.7407407407407408No Hit
ATCAACGGGATTCTTAAAACAACACCCCACTATAATCCTA10.7407407407407408No Hit
ATCAACGGGGCATGTCACCCCAGAGCCCCCTTTTTTTTTT10.7407407407407408No Hit
ATCAACGGGATTGGGGGGGTGATCCCCTCCCACGAGACAC10.7407407407407408No Hit
ATCAACGGGGGCGAGATGCCTCAGTGGGTTAGCGCACCCG10.7407407407407408No Hit
ATCAACGCGATCCGATAACCCCGCCCTAATTCGTATACAC10.7407407407407408No Hit
ATCAACGGGTGGTACAACCAGAGCCCAAAAAAAAAAAAAA10.7407407407407408No Hit
ATCAACCTCTAACCAACCCACAACACCCCAAACCCTTCTT10.7407407407407408No Hit
ATCAACAATCCAACAAACAAAAAAAAAAAAAATCACAAAA10.7407407407407408No Hit
ATCAACAAAAAAAAAAACAAAAAAAAAAAAAAAAAATAAA10.7407407407407408No Hit
ATCAACGGGACCCAGTGGTAACAACCCAAAACAAAACACA10.7407407407407408No Hit
ATCAACGGGAAGACCCCCAGGACTTCTCCATCTTTACCAG10.7407407407407408No Hit
ATCAACGGGAAGCAGTGGTATCAACACAGGGCCGACTTTT10.7407407407407408No Hit
ATCAACGGGGGACGAGAGTAATATTAAAGATGACCCTTCC10.7407407407407408No Hit
ATCAACGGGGTTTCTATGCTGTTCNAANNCTAGCCCGAAG10.7407407407407408No Hit
ATCAACGGGGAAACAACCCAAAAAAAAAAAAAAAAAAAAA10.7407407407407408No Hit
ATCAACGGGCGTGTACTGTTACCCCCACATCCATTTTCCT10.7407407407407408No Hit
ATCAACGGGACTTCGAGAGATTGTATCTTTTTAGTACTTC10.7407407407407408No Hit
ATCAACGGGAACCTGGTGTAACCCAGAGCCGCCTTTTTTT10.7407407407407408No Hit
ATCAACGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT10.7407407407407408No Hit
ATCAACGGGTGTCGCCGAGTCGATTTCCTTTTTTTTTTTT10.7407407407407408No Hit
ATCAACGGGGTCATGCAGCTCGGAAGAGCTCGTATGCCGC10.7407407407407408Illumina Single End Adapter 2 (95% over 23bp)
ATCAACGGGAAGGAACAAAGACTTTCCTAATCTTGAGCAC10.7407407407407408No Hit
ATCAACGGGACTGGGGATGCCCCCCCCCCCCCACACCACG10.7407407407407408No Hit
ATCAACTTGAACTTCTACACCCACCCTCCCCCCTCACATC10.7407407407407408No Hit
ATCAACGGGACATGGTACCCCCCACCGCCGCCTCCTTTCT10.7407407407407408No Hit
ATCAACGGGGTCTTATGTTATTTGTTTTTTTTTTTTTTCC10.7407407407407408No Hit
ATCAACGGGAGTGTTCACCCAGAGCCGACTTTTTTTTTTT10.7407407407407408No Hit
ATCAACGGGGGTACCAGACAGTCAAGTGCCAAATCCAGAG10.7407407407407408No Hit
ATCAACGGGAAACAGTGTCCAACCCGCGCCGCCCTTTTTT10.7407407407407408No Hit
ATCAACGGGCCATCACAACCCAAAGCACCCCCCTCACCAC10.7407407407407408No Hit
ATCAACGGGCTGGTTCAACCAGAGTCGCCCTTTTTTTTTT10.7407407407407408No Hit
ATCAACGGGGGCCTTTTTTTTTTTTTTTTGGTTTTTCCTT10.7407407407407408No Hit
ATCAACAAACATCAACAAAAAAACAAGACCTAAAATATAC10.7407407407407408No Hit
ATCAACGGGACTTGAAAAAAAAAAAAAAAAAAAAAAAAAA10.7407407407407408No Hit
ATCAACGGGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAA10.7407407407407408No Hit
ATCAACCTTACACAACCCCCATCAAACTCTACCTCACACA10.7407407407407408No Hit
ATCAACGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.7407407407407408No Hit
ATCAACGTGATAACATTCATCACACAAGCATTCAATCACA10.7407407407407408No Hit
ATCAACGGGACCGTGTACCCCAGAGCCCCCTTTTTTTTTT10.7407407407407408No Hit
ATCAACTCAAAAAACAAAACCAAACACAACAAAACCTAAA10.7407407407407408No Hit
ATCAACGGGTGGACCGACCTTTTTTTTTTTTTTTTTTTTT10.7407407407407408No Hit
ATCAACGGACCCCTGCCACCCCCACACCCCCCTTTCCTTT10.7407407407407408No Hit
ATCAACGGGCCTCTTCCCATAACACCACCACACGCCCTTC10.7407407407407408No Hit
ATCAACGGGGTTCAGTTTCTACCCTGGCCCACTCCCAGCC10.7407407407407408No Hit
ATCAACGGGACGCATCACTTTTTTTTTTTTTTTTTTTTTT10.7407407407407408No Hit
ATCAACGGGGAAAAAAAACAAAAAAAAACAAAAACAAAAA10.7407407407407408No Hit
ATCAACGGGACCTCCCCAGTCGTAACAAAAAAACAACGAC10.7407407407407408No Hit
ATCAACGGGATTACCCAGAGTCCACTTTTTTTTTTTTTTT10.7407407407407408No Hit
ATCAACGGGGAAATAGGGNATTAAGATTTTAATAGAACAG10.7407407407407408No Hit
ATCAACGGGACCTGTGCAGAGGACAACTGTCCTGTCATCT10.7407407407407408No Hit
ATCAACCCCACCCCCCCCACCACACTCCCCACCTCCTATT10.7407407407407408No Hit
ATCAACGGGGTTTTTGAAATTATTCCACCCACTCAACTTT10.7407407407407408No Hit
ATCAACGGGGCCAGTGTATCAACCCAGCCTCGACTTTTTT10.7407407407407408No Hit
ATCAACTCATCGCAAAAATTTCATCCCAACACTCATTGAC10.7407407407407408No Hit
ATCAACATTTTCTCTCACCACCCCGGCCGACCCTAGCCCC10.7407407407407408No Hit
ATCAACGGGAAGCAGTGGTATCCCCCCAGAGCCCCCTTCT10.7407407407407408No Hit
ATCAACGGACTTTTTTTTTTTTTTTTTTTTTTTTTTCCCC10.7407407407407408No Hit
ATCAACGGGCCTGGTATCCCGAGANTCGATTTTTTTTTTT10.7407407407407408No Hit
ATCAACGGGAAGCCTGTGCGTTATAATAGACTAAGCTCAA10.7407407407407408No Hit
ATCAACAACCCAAACACCTTAACACGCAACAACAAAACCA10.7407407407407408No Hit
ATCAACGGGATACACAGACCTCCCTTCCAAAAAAAAAAAA10.7407407407407408No Hit
ATCAACCCCCACAAATTAAAACTACACTTCAAAAACTAAC10.7407407407407408No Hit
ATCAACGGGATTTAAGTTCCGATACAACCACTAAGAAGAA10.7407407407407408No Hit
ATCAACGGGAAGCCTTCAACCCCGCGCCCCCTTTTTTTTT10.7407407407407408No Hit
ATCAACGGGAGTGCATCAACCCCGCGTCGACTTTTTTTTT10.7407407407407408No Hit
ATCAACGGGCCGAGTCCTTTTTTTTTTTTTTTTTTTTTTT10.7407407407407408No Hit
ATCAACGGGGAGACTGTGTGTTTCCCCCATGCCGGCGGCC10.7407407407407408No Hit
ATCAACGGGAAGAGTGGTATCAACCCAGAGCCGCCTTTTT10.7407407407407408No Hit
ATCAACGGGATGACCACAAATTGCCCCCCTATCAATTTTA10.7407407407407408No Hit
ATCAACCCTCACAAAAATTAAAACACACAAATATCAATTA10.7407407407407408No Hit
ATCAACGGGAATATCGAGAGTCGACTTTTTTTTTTTTTTT10.7407407407407408No Hit
ATCAACGGGACTCCAGTCCATAGCTTCCACCAGCCAGGTT10.7407407407407408No Hit
ATCAACGGGGGAACAAAAAAAAAAAAAAAAAAAAAAAAAA10.7407407407407408No Hit
ATCAACCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCC10.7407407407407408No Hit
ATCAACCACACCAAACAAAACACAACCACAACACATAAAC10.7407407407407408No Hit
ATCAACGCATCCTTCCCCCCCCACTCTACTCGATCCAACT10.7407407407407408No Hit
ATCAACGGGCTTCCCCAGACCGCCTTTTTTTTTTTTCCCT10.7407407407407408No Hit
ATCAACAGGAAGCAGTGTATCAACCCAACACCCCCAACAC10.7407407407407408No Hit
ATCAACAGGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7407407407407408No Hit
ATCAACGGGGTGTCTGAAGAAATCTACTCATACACATAAA10.7407407407407408No Hit
ATCAACGGGGACAAAAAAAAAAAAAAACAAAAAAAAAAAA10.7407407407407408No Hit
ATCAACGGGGGATCAACCCCCACAACACCCCCGCCCCCCC10.7407407407407408No Hit
ATCAACGGGGACTTTTTTTTTTTTTTTTTTTTTTTTCCTC10.7407407407407408No Hit
ATCAACGGGGTGCACTACTATCCACCCACGCGGGGGGGGG10.7407407407407408No Hit
ATCAACGGGCGGTGGGTGCCAAAACGACCTGCTGTTTTTA10.7407407407407408No Hit
ATCAACGGGGCTTACACCGTATGCCCCGCGCCATTAGCTA10.7407407407407408No Hit
ATCAACAATAACCCAATATACCCCCCCCCCATCCCATCCA10.7407407407407408No Hit
ATCAACGGGGCAGAGTCGACTTTTTTTTTCTTTTTTTTTT10.7407407407407408No Hit
ATCAACCCACCCACCAACCCAACACACACAACTACCCACT10.7407407407407408No Hit
ATCAACGGGGCAGTGGTATTCAACCCCGAGACCCGTGCTA10.7407407407407408No Hit
ATCAACGGGCGAGTTTTTTTTTTTTTTTTTTTCCCAAAAA10.7407407407407408No Hit
ATCAACGGATTTGAGAATAAATAAATATTGAATTTTTAAA10.7407407407407408No Hit
ATCAACGGGAAGAGTGGATAACCCGCCCGATTTTTTTTTT10.7407407407407408No Hit
ATCAACGGGGAGTTGGAGAGGGCCGTCCCCGAGAAGTTAC10.7407407407407408No Hit
ATCAACGGGAGACCGCAGAGCGATTTTTTTTTTTTTTTTT10.7407407407407408No Hit
ATCAACGGGGCTATCACCCCCCCCCCCCTGGCGATGAGAT10.7407407407407408No Hit
ATCAACGCAGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT10.7407407407407408No Hit
ATCAACGTTCTTCACCCCCCCTACTTCGTCTTTTTCTTTT10.7407407407407408No Hit
ATCAACGATTCCCTCCCGCCAGGCTCCCTGATGTCCGTCG10.7407407407407408No Hit
ATCAACGGGGTTTCAACGCGAGCCGCCTTTTTTTTTTTTT10.7407407407407408No Hit
ATCAACCACCAACCACACCCCAACCACAAATAACACCTCC10.7407407407407408No Hit
ATCAACGGGGTGATATTAAAATCATTAAAGGGTTTTCCCC10.7407407407407408No Hit
ATCAACGGGAAGTGGATCCACCCCGCGCCCCCTTTTTTTT10.7407407407407408No Hit
ATCAACGGGGGGCTGGTGCGATGGCCCAGTGGGTAAGAGC10.7407407407407408No Hit
ATCAACACACGCCCTCAGCCTTATGCCATTACACTCGTCA10.7407407407407408No Hit
ATCAACCACACCCCACCCACCCCAACCAACTGATAATTAC10.7407407407407408No Hit
ATCAACGGGGAGTCTGGCCGCACCCGCCGCCCCCCGCCCG10.7407407407407408No Hit
ATCAACGGGGTACAGAGCGATTTTTTTTTTTTTTTTTTTT10.7407407407407408No Hit
ATCAACAAAACAACAACAACACCCCCCTCACATCACATTC10.7407407407407408No Hit
ATCAACGGGACCGTGGTCAACCCAGAGCCGACTTTTTTTT10.7407407407407408No Hit
ATCAACAACACACCCCAACACCCAAAAAAAAACAACCCCA10.7407407407407408No Hit
ATCAACGGGCCTCATTCCCGACAACCCTACTAAAAACTTA10.7407407407407408No Hit
ATCAACGGGGATGAAGTGCTAATGTAACATCAGTACTTAT10.7407407407407408No Hit
ATCAACGGGTTACCGAGCCTTCTTCGGCGGGGGGGCGGGG10.7407407407407408No Hit
ATCAACGGGACGCATGGATCAACCCAGCGCCCACTTTTTT10.7407407407407408No Hit
ATCAACGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT10.7407407407407408No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers