FastQCFastQC Report
Fri 17 Jun 2016
SRR1525807_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525807_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1853
Sequences flagged as poor quality0
Sequence length40
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT180.9713977334052887No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC130.7015650296815974No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG100.5396654074473827No Hit
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT100.5396654074473827No Hit
ATCAACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG90.48569886670264434No Hit
ATCAACGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGA80.4317323259579061No Hit
ATCAACGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG70.37776578521316784No Hit
ATCAACGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT70.37776578521316784No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC50.26983270372369134No Hit
ATCAACGGGGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA50.26983270372369134No Hit
ATCAACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.21586616297895306No Hit
ATCAACGGGAACTGTGGTAATTCTAGAGCTAATACATGCC40.21586616297895306No Hit
ATCAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.21586616297895306No Hit
ATCAACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA40.21586616297895306No Hit
ATCAACGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG30.16189962223421478No Hit
ATCAACGGGACTTACTTATTTATATATTTATTTGATTGTT30.16189962223421478No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG30.16189962223421478No Hit
ATCAACGGGGGATCGGTGATGTTCACAGTTCTCCCTGTGC30.16189962223421478No Hit
ATCAACGGGGGAAAACTTGAGGTTGGAAAATGAATGAAAA30.16189962223421478No Hit
ATCAACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT30.16189962223421478No Hit
ATCAACGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA30.16189962223421478No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC30.16189962223421478No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA30.16189962223421478No Hit
ATCAACGGGGGTTCGATTCCGGAGAGGGAGCCTGAGAAAC30.16189962223421478No Hit
ATCAACGGGATTCGTAGGAGTAAACATAACATTCTTCCCT30.16189962223421478No Hit
ATCAACGGGTGCAGTGCTATCAACGGGGTTGGGCACGGAA20.10793308148947653No Hit
ATCAACGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10793308148947653No Hit
ATCAACGGGGCTTCTGTAGCTTCTACTGTCCCTGTGGCTA20.10793308148947653No Hit
ATCAACGGGCTCTAAGAGCCATTCTGGGCAAGTGAAGGGC20.10793308148947653No Hit
ATCAACGGGGTCATTTGGCTGCTACTTGCTTAGTGACTCC20.10793308148947653No Hit
ATCAACGGGGTCTTATGTAGTTCAGTCTGGCCTTCAATTC20.10793308148947653No Hit
ATCAACGGGGGGCATGGGATGCTGGGTGGGTGAACTACAC20.10793308148947653No Hit
ATCAACGAGGACTTATGAATGATTGATTTGACAGAGACCC20.10793308148947653No Hit
ATCAACGGGGGTGTGAATGTTGCCGGCGTCTCCCTGAAGT20.10793308148947653No Hit
ATCAACGGGACTCCTATTCTGCCTAGCAAACTCCAACTAC20.10793308148947653No Hit
ATCAACGGGTCAAAGATACTCTTCTGAGATGAGTTTTTGT20.10793308148947653No Hit
ATCAACGGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCC20.10793308148947653No Hit
ATCAACGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGA20.10793308148947653No Hit
ATCAACGGGCTCCTTGTTGAAAAAAAAAAAAAAAAAAAAA20.10793308148947653No Hit
ATCAACGGGGGTTGATCCTGCCAGTAGCATATGCTTGTCT20.10793308148947653No Hit
ATCAACGGGAGTGCAGTGCTATCAACGGGTCACAGATCCT20.10793308148947653No Hit
ATCAACGGGGCTCGCTCCTCTCCTACTTGGATAACTGTGG20.10793308148947653No Hit
ATCAACGGGGGGTACGATTCCGGAGAGGGAGCCTGAGAAA20.10793308148947653No Hit
ATCAACGGGAGATTAGTGTCTAACATGCTAGCTACTCAGT20.10793308148947653No Hit
ATCAACGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGG20.10793308148947653No Hit
ATCAACGGGGCTTTTGCTTGGGATCAGTGCTCTGCTAACG20.10793308148947653No Hit
ATCAACGGGTCACAGATCCTCTTATGAGATGAGTTTTTGT20.10793308148947653No Hit
ATCAACGGGAGCATATGCTTGTCTCAAAGATTAAGCCATG20.10793308148947653No Hit
ATCAACGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAAT20.10793308148947653No Hit
ATCAACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTT20.10793308148947653No Hit
ATCAACGGGGGGGTTTAATGAGGTGACCTGTAGCTTTGGC20.10793308148947653No Hit
ATCAACGGGGTTTGTTTTATTTTGTTCTAAAATTAAAAGT20.10793308148947653No Hit
ATCAACGGGTCACAAATCCTCTTCTGAAGGAGATTGGGCG20.10793308148947653No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAAAG20.10793308148947653No Hit
ATCAACGGGGAAAGTTTTATTTATAATATGCATTTTTTTT20.10793308148947653No Hit
ATCAACGGGGAAAGAGATTATACTTTCTACATTTGGCAGG20.10793308148947653No Hit
ATCAACGGGATCTTGGGGCAAAAGAAAACAAAAGCAGAAA20.10793308148947653No Hit
ATCAACGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT20.10793308148947653No Hit
ATCAACGGGAGGGGGAAGAGTGGGAAAACCTTTCAAGGAA20.10793308148947653No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGC20.10793308148947653No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG20.10793308148947653No Hit
ATCAACGGGGGGTTCGATTCCGGAGAGGGAGCCTAAGAAA20.10793308148947653No Hit
ATCAACGGGATGTAGTTACTTACTACTCCGGAAAAAAAGA20.10793308148947653No Hit
ATCAACGGGGAAAGCGTAGGGCTTGGGGGTTGGTCTGGTC20.10793308148947653No Hit
ATCAACGGGGGTTTGGTTTTTGTTTTTGTTTTTCCTTTAA20.10793308148947653No Hit
ATCAACGGGGGGGTTGGGAATTTAGCTCAGTGGTAGAACG20.10793308148947653No Hit
ATCAACGGGGTCTAAGTACGCACGGCCGGTACAGTGAAAC20.10793308148947653No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGC20.10793308148947653No Hit
ATCAACGGGAGGCGCGCAAATTACCCACTCCCGACCCGGG20.10793308148947653No Hit
ATCAACGGGAGGCCCTGTAATTGGAATGAGTCCACTTTAA20.10793308148947653No Hit
ATCAACGGGGGGAGCAGTGTTTGCTATCATAGCAGGATTT20.10793308148947653No Hit
ATCAACGGGGAAATCCTGATTCCAGGACTCAGGCCAGCCT20.10793308148947653No Hit
ATCAACGGGGCTTATCCTTCTGTATCACCAATACCATCGC20.10793308148947653No Hit
ATCAACGGGGTCTCTTCTATAGGATCATTTATTTCACTAA20.10793308148947653No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAAAA20.10793308148947653No Hit
ATCAACGGGACCCACTCCCGACCCGGGGAGGTAGTGACGA20.10793308148947653No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG20.10793308148947653No Hit
ATCAACGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC20.10793308148947653No Hit
ATCAACGGGATGGGAAGCCCTTACAGAGGGGCTTCTCCAA20.10793308148947653No Hit
ATCAACGGGCATCCAGATGACCACGACACTCGGTGAACAT20.10793308148947653No Hit
ATCAACGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTC20.10793308148947653No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT20.10793308148947653No Hit
ATCAACGGGACTGTTTATTTGCAGATGTTTGACAAAGGGA20.10793308148947653No Hit
ATCAACGGGTGAGAGGAATGATAGCAACCTGCCTCTATTT20.10793308148947653No Hit
ATCAACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGAG20.10793308148947653No Hit
ATCAACGGGATGGTTGACATTTTGCAGAATAACTCTAATG20.10793308148947653No Hit
ATCAACGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT20.10793308148947653No Hit
ATCAACGGGATAGCAATAATAGCTCTACTAAACCTATTCT20.10793308148947653No Hit
ATCAACGGGGGCTTTGGTGAATCTAGATAACCTCGGGCCG20.10793308148947653No Hit
ATCAACGGGATGTTATAGCTTAACAGAATTACTAATTTCG20.10793308148947653No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG200.002132412734.06
ACGGGTA200.002132412734.05
ACGGGGG251.3509655E-434.05
ACGGGAT200.002132412734.05
AACGGGG800.034.04
AACGGGC200.002132412734.04
AACGGGA451.6807462E-934.04
TCAACGG1950.032.256412
ATCAACG1950.032.256411
CAACGGG1950.032.256413
ACGGGGA401.4356501E-629.755
AACGGGT502.113029E-727.1999994
Produced by FastQC (version 0.11.5)