##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525807_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1853 Sequences flagged as poor quality 0 Sequence length 40 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 17.45979492714517 22.0 4.0 29.0 4.0 32.0 2 13.798704803022126 11.0 4.0 24.0 4.0 29.0 3 10.65353480841878 6.0 4.0 17.0 4.0 22.0 4 11.63086886130599 8.0 4.0 19.0 4.0 26.0 5 12.467350242849433 8.0 4.0 21.0 4.0 29.0 6 12.6389638424177 5.0 4.0 22.0 4.0 28.0 7 14.170534268753373 4.0 4.0 26.0 4.0 30.0 8 14.705882352941176 4.0 4.0 26.0 4.0 30.0 9 14.368051807879114 4.0 4.0 25.0 4.0 30.0 10 12.649757150566648 4.0 4.0 22.0 4.0 29.0 11 14.663788451160281 4.0 4.0 29.0 4.0 34.0 12 14.089044792228819 4.0 4.0 27.0 4.0 33.0 13 13.105774419859687 4.0 4.0 25.0 4.0 32.0 14 13.354020507285483 4.0 4.0 26.0 4.0 33.0 15 13.79816513761468 4.0 4.0 28.0 4.0 33.0 16 11.390717754991906 4.0 4.0 20.0 4.0 30.0 17 10.733405288720993 4.0 4.0 18.0 4.0 29.0 18 10.351322180248246 4.0 4.0 17.0 4.0 28.0 19 10.29465731246627 4.0 4.0 16.0 4.0 28.0 20 10.878035617916892 4.0 4.0 19.0 4.0 30.0 21 9.246087425796006 4.0 4.0 13.0 4.0 26.0 22 9.289800323799245 4.0 4.0 13.0 4.0 25.0 23 9.498111171073933 4.0 4.0 14.0 4.0 26.0 24 8.833243389098758 4.0 4.0 11.0 4.0 24.0 25 9.530491095520777 4.0 4.0 14.0 4.0 27.0 26 9.704803022126281 4.0 4.0 14.0 4.0 28.0 27 9.940636805180787 4.0 4.0 15.0 4.0 29.0 28 10.322719913653534 4.0 4.0 17.0 4.0 29.0 29 9.114409066378846 4.0 4.0 12.0 4.0 26.0 30 9.441985968699406 4.0 4.0 13.0 4.0 27.0 31 10.671883432271992 4.0 4.0 18.0 4.0 31.0 32 10.494333513221802 4.0 4.0 16.0 4.0 31.0 33 10.352941176470589 4.0 4.0 16.0 4.0 31.0 34 10.64543982730707 4.0 4.0 17.0 4.0 32.0 35 10.322719913653534 4.0 4.0 14.0 4.0 32.0 36 10.259039395574744 4.0 4.0 4.0 4.0 33.0 37 10.223961144090664 4.0 4.0 4.0 4.0 33.0 38 9.837021046950891 4.0 4.0 4.0 4.0 32.0 39 9.494333513221802 4.0 4.0 4.0 4.0 31.0 40 9.765785213167836 4.0 4.0 4.0 4.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 16.50345136634199 1 2 20.710117356601728 1 3 19.891526312229438 1 4 17.023882237554112 1 5 18.644257305194802 1 6 14.774379396645022 1 7 21.40100615530303 1 8 12.388844358766235 1 9 10.25993641774892 1 10 17.741730925324674 1 11 17.322590300324677 1 12 17.005450148809526 1 13 22.40624154491342 1 14 8.759496753246754 1 15 6.643743235930737 1 16 2.152807088744586 1 17 -2.1701298701298697 1 18 4.85882711038961 1 19 10.114277259199133 1 20 8.507416801948054 1 21 -4.050157264610389 1 22 -3.741429924242423 1 23 -3.696054856601732 1 24 -3.835868168290043 1 25 -4.21196056547619 1 26 -4.731390354437229 1 27 -4.655012175324677 1 28 -4.987001149891775 1 29 -4.339402394480519 1 30 -4.076092397186148 1 31 -5.032350852272728 1 32 -4.806199269480519 1 33 -4.833590367965368 1 34 -4.84529558982684 1 35 -5.107398200757576 1 36 -4.500985863095238 1 37 -4.894876217532467 1 38 -4.326734983766235 1 39 -3.8040449134199132 1 40 -4.23871922348485 2 1 -9.996548633658008 2 2 -7.78988264339827 2 3 -4.608473687770562 2 4 -5.976117762445888 2 5 -6.355742694805198 2 6 -5.725620603354978 2 7 -7.0989938446969685 2 8 -7.611155641233765 2 9 -7.24006358225108 2 10 -5.758269074675324 2 11 -7.677409699675323 2 12 -6.494549851190474 2 13 -6.593758455086581 2 14 -6.740503246753246 2 15 -6.356256764069263 2 16 -5.347192911255414 2 17 -5.17012987012987 2 18 -5.64117288961039 2 19 -5.385722740800867 2 20 -5.992583198051946 2 21 -4.050157264610389 2 22 -3.741429924242423 2 23 -3.696054856601732 2 24 -3.835868168290043 2 25 -4.21196056547619 2 26 -4.731390354437229 2 27 -4.655012175324677 2 28 -4.987001149891775 2 29 -4.339402394480519 2 30 -4.076092397186148 2 31 -5.032350852272728 2 32 -4.806199269480519 2 33 -4.833590367965368 2 34 -4.84529558982684 2 35 -5.107398200757576 2 36 -4.500985863095238 2 37 -4.894876217532467 2 38 -4.326734983766235 2 39 -3.8040449134199132 2 40 -4.23871922348485 3 1 -9.996548633658008 3 2 -7.78988264339827 3 3 -4.608473687770562 3 4 -5.976117762445888 3 5 -6.355742694805198 3 6 -5.725620603354978 3 7 -7.0989938446969685 3 8 -7.611155641233765 3 9 -7.24006358225108 3 10 -5.758269074675324 3 11 -7.677409699675323 3 12 -6.494549851190474 3 13 -6.593758455086581 3 14 -6.740503246753246 3 15 -6.356256764069263 3 16 -5.347192911255414 3 17 -5.17012987012987 3 18 -5.64117288961039 3 19 -5.385722740800867 3 20 -5.992583198051946 3 21 -4.050157264610389 3 22 -3.741429924242423 3 23 -3.696054856601732 3 24 -3.835868168290043 3 25 -4.21196056547619 3 26 -4.731390354437229 3 27 -4.655012175324677 3 28 -4.987001149891775 3 29 -4.339402394480519 3 30 -4.076092397186148 3 31 -5.032350852272728 3 32 -4.806199269480519 3 33 -4.833590367965368 3 34 -4.84529558982684 3 35 -5.107398200757576 3 36 -4.500985863095238 3 37 -4.894876217532467 3 38 -4.326734983766235 3 39 -3.8040449134199132 3 40 -4.23871922348485 4 1 14.003451366341992 4 2 23.210117356601728 4 3 17.391526312229438 4 4 20.023882237554112 4 5 24.644257305194802 4 6 14.274379396645022 4 7 12.901006155303032 4 8 19.388844358766235 4 9 12.75993641774892 4 10 2.241730925324676 4 11 19.322590300324677 4 12 21.505450148809526 4 13 20.40624154491342 4 14 8.259496753246754 4 15 10.643743235930737 4 16 5.652807088744586 4 17 19.82987012987013 4 18 4.35882711038961 4 19 16.614277259199135 4 20 21.007416801948054 4 21 24.94984273538961 4 22 21.258570075757575 4 23 21.303945143398266 4 24 26.16413183170996 4 25 23.78803943452381 4 26 24.26860964556277 4 27 21.34498782467532 4 28 18.012998850108225 4 29 17.66059760551948 4 30 21.923907602813852 4 31 20.967649147727272 4 32 14.19380073051948 4 33 26.166409632034632 4 34 9.15470441017316 4 35 23.892601799242424 4 36 -4.500985863095238 4 37 -4.894876217532467 4 38 -4.326734983766235 4 39 -3.8040449134199132 4 40 -4.23871922348485 6 1 17.25345136634199 6 2 18.710117356601728 6 3 -1.1084736877705623 6 4 7.773882237554112 6 5 17.644257305194802 6 6 12.774379396645022 6 7 5.9010061553030315 6 8 12.388844358766235 6 9 14.75993641774892 6 10 5.491730925324676 6 11 8.572590300324677 6 12 17.005450148809526 6 13 12.656241544913419 6 14 9.009496753246754 6 15 11.893743235930737 6 16 10.402807088744586 6 17 12.07987012987013 6 18 8.10882711038961 6 19 13.364277259199133 6 20 12.507416801948054 6 21 6.199842735389611 6 22 6.258570075757577 6 23 9.553945143398268 6 24 6.164131831709957 6 25 8.78803943452381 6 26 12.018609645562771 6 27 14.094987824675323 6 28 10.762998850108225 6 29 11.66059760551948 6 30 13.423907602813852 6 31 13.217649147727272 6 32 15.69380073051948 6 33 8.416409632034632 6 34 8.15470441017316 6 35 9.892601799242424 6 36 9.249014136904762 6 37 8.605123782467533 6 38 6.923265016233765 6 39 4.195955086580087 6 40 8.26128077651515 7 1 2.40345136634199 7 2 5.810117356601731 7 3 3.9915263122294373 7 4 2.223882237554111 7 5 2.244257305194802 7 6 4.474379396645022 7 7 -2.498993844696969 7 8 3.7888443587662355 7 9 4.359936417748919 7 10 4.041730925324677 7 11 -5.077409699675323 7 12 8.305450148809527 7 13 4.606241544913418 7 14 -0.1405032467532461 7 15 3.2437432359307365 7 16 7.052807088744585 7 17 4.229870129870131 7 18 8.15882711038961 7 19 6.614277259199133 7 20 1.0074168019480538 7 21 8.549842735389612 7 22 8.258570075757577 7 23 8.90394514339827 7 24 8.164131831709957 7 25 9.188039434523809 7 26 8.868609645562772 7 27 8.744987824675322 7 28 8.812998850108226 7 29 3.86059760551948 7 30 8.32390760281385 7 31 2.3676491477272723 7 32 5.593800730519481 7 33 2.566409632034633 7 34 0.9547044101731608 7 35 0.8926017992424242 7 36 1.6990141369047613 7 37 5.705123782467533 7 38 7.8732650162337645 7 39 4.795955086580086 7 40 6.36128077651515 8 1 0.8605942234848492 8 2 -2.9327397862554125 8 3 3.962954883658009 8 4 -2.2618320481601737 8 5 -2.070028409090911 8 6 0.9886651109307358 8 7 5.758149012445889 8 8 4.388844358766235 8 9 5.902793560606062 8 10 2.813159496753247 8 11 -0.10598112824675177 8 12 1.6483072916666686 8 13 -0.02232988365800992 8 14 3.116639610389612 8 15 4.786600378787879 8 16 5.509949945887444 8 17 3.544155844155844 8 18 3.787398538961039 8 19 -1.2428655979437249 8 20 5.150273944805196 8 21 3.092699878246753 8 22 3.6871415043290057 8 23 3.8753737148268392 8 24 -2.6930110254328996 8 25 3.216610863095239 8 26 7.982895359848486 8 27 6.487844967532466 8 28 7.012998850108225 8 29 3.9463118912337674 8 30 7.495336174242423 8 31 7.681934862012987 8 32 6.336657873376623 8 33 10.02355248917749 8 34 10.297561553030302 8 35 7.892601799242424 8 36 11.213299851190477 8 37 6.962266639610391 8 38 6.958979301948052 8 39 6.481669372294373 8 40 9.046995062229435 9 1 7.0034513663419915 9 2 4.21011735660173 9 3 1.3915263122294377 9 4 16.023882237554112 9 5 -0.3557426948051976 9 6 0.2743793966450223 9 7 9.901006155303032 9 8 14.388844358766235 9 9 13.75993641774892 9 10 7.241730925324676 9 11 18.322590300324677 9 12 5.505450148809526 9 13 5.406241544913419 9 14 15.259496753246754 9 15 10.643743235930737 9 16 14.652807088744586 9 17 19.82987012987013 9 18 11.35882711038961 9 19 21.614277259199135 9 20 21.007416801948054 9 21 12.94984273538961 9 22 4.258570075757577 9 23 8.303945143398268 9 24 11.164131831709957 9 25 10.78803943452381 9 26 8.268609645562771 9 27 8.344987824675323 9 28 22.012998850108225 9 29 8.66059760551948 9 30 8.923907602813852 9 31 20.967649147727272 9 32 7.193800730519481 9 33 7.166409632034632 9 34 21.15470441017316 9 35 20.892601799242424 9 36 23.499014136904762 9 37 25.105123782467533 9 38 15.673265016233765 9 39 14.195955086580087 9 40 23.76128077651515 10 1 5.0034513663419915 10 2 11.21011735660173 10 3 8.391526312229438 10 4 11.023882237554112 10 5 5.644257305194802 10 6 2.2743793966450223 10 7 6.9010061553030315 10 8 12.388844358766235 10 9 11.75993641774892 10 10 -3.758269074675324 10 11 19.322590300324677 10 12 11.505450148809526 10 13 16.40624154491342 10 14 7.259496753246754 10 15 13.643743235930737 10 16 17.652807088744588 10 17 14.82987012987013 10 18 23.358827110389612 10 19 23.614277259199135 10 20 22.007416801948054 10 21 9.94984273538961 10 22 -2.741429924242423 10 23 6.303945143398268 10 24 0.16413183170995715 10 25 20.78803943452381 10 26 5.268609645562771 10 27 8.344987824675323 10 28 11.012998850108225 10 29 6.660597605519481 10 30 1.923907602813852 10 31 14.967649147727272 10 32 22.19380073051948 10 33 18.166409632034632 10 34 13.15470441017316 10 35 19.892601799242424 10 36 18.499014136904762 10 37 29.105123782467533 10 38 29.673265016233763 10 39 27.195955086580085 10 40 26.76128077651515 11 1 7.670118033008659 11 2 1.8767840232683959 11 3 6.724859645562772 11 4 2.357215570887446 11 5 10.644257305194802 11 6 11.94104606331169 11 7 11.901006155303032 11 8 1.0555110254329012 11 9 1.4266030844155857 11 10 3.908397591991342 11 11 0.655923633658011 11 12 3.505450148809526 11 13 -6.593758455086581 11 14 -6.740503246753246 11 15 -6.356256764069263 11 16 -5.347192911255414 11 17 -5.17012987012987 11 18 -5.64117288961039 11 19 -5.385722740800867 11 20 -5.992583198051946 11 21 -4.050157264610389 11 22 -3.741429924242423 11 23 -3.696054856601732 11 24 -3.835868168290043 11 25 -4.21196056547619 11 26 -4.731390354437229 11 27 -4.655012175324677 11 28 -4.987001149891775 11 29 -4.339402394480519 11 30 -4.076092397186148 11 31 -5.032350852272728 11 32 -4.806199269480519 11 33 -4.833590367965368 11 34 -4.84529558982684 11 35 -5.107398200757576 11 36 -4.500985863095238 11 37 -4.894876217532467 11 38 -4.326734983766235 11 39 -3.8040449134199132 11 40 -4.23871922348485 12 1 21.00345136634199 12 2 24.210117356601728 12 3 27.391526312229438 12 4 9.023882237554112 12 5 8.644257305194802 12 6 26.274379396645024 12 7 12.901006155303032 12 8 11.388844358766235 12 9 17.759936417748918 12 10 25.241730925324674 12 11 12.322590300324677 12 12 13.505450148809526 12 13 22.40624154491342 12 14 25.259496753246754 12 15 12.643743235930737 12 16 12.652807088744586 12 17 9.82987012987013 12 18 19.358827110389612 12 19 1.6142772591991328 12 20 12.007416801948054 12 21 3.949842735389611 12 22 7.258570075757577 12 23 3.303945143398268 12 24 18.16413183170996 12 25 7.78803943452381 12 26 19.26860964556277 12 27 1.3449878246753233 12 28 2.012998850108225 12 29 22.66059760551948 12 30 19.923907602813852 12 31 20.967649147727272 12 32 26.19380073051948 12 33 9.166409632034632 12 34 24.15470441017316 12 35 23.892601799242424 12 36 8.499014136904762 12 37 21.105123782467533 12 38 -4.326734983766235 12 39 -3.8040449134199132 12 40 -4.23871922348485 13 1 7.367087729978355 13 2 3.482844629329003 13 3 3.0278899485930744 13 4 2.2966095102813853 13 5 1.826075487012984 13 6 6.365288487554112 13 7 5.628278882575758 13 8 5.570662540584419 13 9 6.214481872294373 13 10 4.878094561688313 13 11 8.958953936688314 13 12 5.505450148809526 13 13 2.769877908549782 13 14 4.623133116883118 13 15 2.7346523268398286 13 16 3.9255343614718594 13 17 0.3753246753246753 13 18 0.7224634740259734 13 19 -2.022086377164504 13 20 1.8255986201298722 13 21 0.31347909902597415 13 22 1.0767518939393952 13 23 3.0312178706709947 13 24 0.9823136498917755 13 25 3.78803943452381 13 26 0.9049732819264076 13 27 4.708624188311687 13 28 2.55845339556277 13 29 2.4787794237012992 13 30 2.1057257846320336 13 31 3.513103693181817 13 32 2.0119825487012992 13 33 3.6209550865800875 13 34 2.4274316829004334 13 35 3.074419981060606 13 36 1.499014136904762 13 37 0.74148741883117 13 38 2.4914468344155836 13 39 2.55959145021645 13 40 2.397644412878787 15 1 4.5034513663419915 15 2 4.46011735660173 15 3 -3.1084736877705623 15 4 3.273882237554112 15 5 7.394257305194802 15 6 12.524379396645022 15 7 12.401006155303032 15 8 9.638844358766235 15 9 4.25993641774892 15 10 3.741730925324676 15 11 1.072590300324677 15 12 7.505450148809526 15 13 4.156241544913419 15 14 -0.7405032467532457 15 15 4.893743235930737 15 16 4.902807088744586 15 17 4.82987012987013 15 18 3.35882711038961 15 19 4.364277259199133 15 20 2.257416801948054 15 21 7.949842735389611 15 22 8.258570075757577 15 23 6.553945143398268 15 24 4.164131831709957 15 25 2.78803943452381 15 26 4.768609645562771 15 27 5.094987824675323 15 28 8.012998850108225 15 29 3.660597605519481 15 30 6.923907602813852 15 31 6.467649147727272 15 32 3.193800730519481 15 33 5.166409632034632 15 34 6.40470441017316 15 35 -0.6073982007575758 15 36 1.499014136904762 15 37 0.8551237824675333 15 38 2.9232650162337652 15 39 1.6959550865800868 15 40 1.7612807765151501 16 1 -0.9965486336580085 16 2 -0.2898826433982702 16 3 -3.6084736877705623 16 4 1.523882237554112 16 5 -0.8557426948051976 16 6 -4.725620603354978 16 7 4.4010061553030315 16 8 2.388844358766235 16 9 2.7599364177489196 16 10 -1.758269074675324 16 11 7.322590300324677 16 12 8.505450148809526 16 13 8.406241544913419 16 14 8.259496753246754 16 15 8.143743235930737 16 16 -0.34719291125541396 16 17 0.8298701298701303 16 18 3.35882711038961 16 19 0.6142772591991328 16 20 6.507416801948054 16 21 1.9498427353896108 16 22 7.758570075757577 16 23 8.303945143398268 16 24 7.164131831709957 16 25 3.28803943452381 16 26 4.768609645562771 16 27 4.844987824675323 16 28 5.012998850108225 16 29 1.6605976055194809 16 30 2.923907602813852 16 31 2.967649147727272 16 32 7.693800730519481 16 33 6.666409632034632 16 34 8.15470441017316 16 35 10.392601799242424 16 36 8.999014136904762 16 37 10.605123782467533 16 38 11.173265016233765 16 39 11.695955086580087 16 40 11.26128077651515 17 1 13.003451366341992 17 2 12.924403070887445 17 3 7.53438345508658 17 4 4.166739380411254 17 5 8.644257305194802 17 6 13.417236539502165 17 7 12.043863298160174 17 8 12.245987215909093 17 9 11.75993641774892 17 10 7.813159496753249 17 11 18.89401887175325 17 12 18.505450148809526 17 13 13.263384402056277 17 14 17.830925324675327 17 15 20.643743235930735 17 16 13.509949945887444 17 17 13.258441558441557 17 18 13.501684253246752 17 19 12.185705830627706 17 20 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16 -5.347192911255414 117 17 -5.17012987012987 117 18 -5.64117288961039 117 19 -5.385722740800867 117 20 -5.992583198051946 117 21 -4.050157264610389 117 22 -3.741429924242423 117 23 -3.696054856601732 117 24 -3.835868168290043 117 25 -4.21196056547619 117 26 -4.731390354437229 117 27 -4.655012175324677 117 28 -4.987001149891775 117 29 -4.339402394480519 117 30 -4.076092397186148 117 31 -5.032350852272728 117 32 -4.806199269480519 117 33 -4.833590367965368 117 34 -4.84529558982684 117 35 -5.107398200757576 117 36 -4.500985863095238 117 37 -4.894876217532467 117 38 -4.326734983766235 117 39 -3.8040449134199132 117 40 -4.23871922348485 120 1 -9.996548633658008 120 2 -7.78988264339827 120 3 -4.608473687770562 120 4 -5.976117762445888 120 5 -6.355742694805198 120 6 -5.725620603354978 120 7 -7.0989938446969685 120 8 -7.611155641233765 120 9 -7.24006358225108 120 10 -5.758269074675324 120 11 -7.677409699675323 120 12 -6.494549851190474 120 13 -6.593758455086581 120 14 -6.740503246753246 120 15 -6.356256764069263 120 16 -5.347192911255414 120 17 -5.17012987012987 120 18 -5.64117288961039 120 19 -5.385722740800867 120 20 -5.992583198051946 120 21 -4.050157264610389 120 22 -3.741429924242423 120 23 -3.696054856601732 120 24 -3.835868168290043 120 25 -4.21196056547619 120 26 -4.731390354437229 120 27 -4.655012175324677 120 28 -4.987001149891775 120 29 -4.339402394480519 120 30 -4.076092397186148 120 31 -5.032350852272728 120 32 -4.806199269480519 120 33 -4.833590367965368 120 34 -4.84529558982684 120 35 -5.107398200757576 120 36 -4.500985863095238 120 37 -4.894876217532467 120 38 -4.326734983766235 120 39 -3.8040449134199132 120 40 -4.23871922348485 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 859.0 5 28.0 6 31.0 7 30.0 8 13.0 9 54.0 10 65.0 11 60.0 12 54.0 13 30.0 14 31.0 15 24.0 16 21.0 17 13.0 18 17.0 19 38.0 20 58.0 21 72.0 22 90.0 23 86.0 24 60.0 25 43.0 26 31.0 27 19.0 28 12.0 29 10.0 30 1.0 31 1.0 32 1.0 33 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 98.70270270270271 0.8108108108108109 0.21621621621621623 0.2702702702702703 8 96.76025917926566 1.2419006479481642 0.9719222462203023 1.0259179265658747 9 97.19373988127361 1.4031300593631948 1.1872638963842417 0.21586616297895306 10 54.93793847814356 20.72315164597949 14.624932541824068 9.713977334052887 11 35.745140388768895 26.13390928725702 18.628509719222464 19.49244060475162 12 33.67512142471668 24.338909875876958 22.288181327576904 19.697787371829463 13 23.63734484619536 22.234214786832162 27.576902320561253 26.551538046411228 14 19.27645788336933 30.72354211663067 29.481641468682508 20.518358531317496 15 30.329195898542906 26.17377226119806 27.684835402050727 15.812196438208312 16 30.059363194819213 28.98003237992445 26.281705342687534 14.678899082568808 17 28.023758099352055 25.377969762419006 31.641468682505398 14.956803455723541 18 25.25634106853751 28.116567728008633 31.13869400971398 15.48839719373988 19 23.475445223961145 29.897463572584996 23.63734484619536 22.9897463572585 20 22.9897463572585 28.27846735024285 32.54182406907717 16.189962223421478 21 23.04371289800324 27.145169994603346 29.249865083648135 20.561252023745276 22 20.23745277927685 24.824608742579603 36.805180787911496 18.132757690232058 23 20.669185105234757 26.065839179708583 35.509983810037774 17.754991905018887 24 20.885051268213708 29.789530491095523 29.14193200215866 20.18348623853211 25 23.933009184224744 29.605618584548893 27.336574824419234 19.124797406807133 26 27.410617551462625 30.390032502708557 22.697724810400864 19.501625135427954 27 20.347259902333153 32.01302224633749 25.339120998372216 22.300596852957135 28 21.934089681253376 31.766612641815232 24.365207995678013 21.934089681253376 29 26.93347755543537 31.530557057869117 23.201730665224446 18.334234721471066 30 24.946004319654428 30.561555075593954 28.293736501079913 16.19870410367171 31 26.551538046411228 29.627630868861303 27.25310307609282 16.567728008634646 32 28.773072747014115 29.09880564603692 25.624321389793703 16.503800217155266 33 26.551538046411228 27.792768483540204 27.576902320561253 18.078791149487316 34 21.586616297895304 27.361036157582298 35.4020507285483 15.650296815974096 35 20.23745277927685 30.167296276308686 32.32595790609822 17.269293038316246 36 20.30319436924743 32.051976177585274 29.66973470492691 17.975094748240387 37 23.691311386940097 31.13869400971398 28.116567728008633 17.05342687533729 38 23.745277927684835 33.78305450620615 25.634106853750676 16.83756071235834 39 25.20237452779277 31.94819212088505 22.288181327576904 20.561252023745276 40 21.154883971937398 29.411764705882355 28.656233135456016 20.77711818672423 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 1.0 10 1.0 11 3.5 12 6.0 13 6.0 14 9.0 15 12.0 16 15.0 17 18.0 18 18.0 19 16.0 20 14.0 21 17.5 22 21.0 23 21.0 24 24.0 25 27.0 26 29.5 27 32.0 28 32.0 29 35.0 30 38.0 31 54.0 32 70.0 33 70.0 34 72.0 35 74.0 36 87.0 37 100.0 38 100.0 39 108.5 40 117.0 41 135.5 42 154.0 43 154.0 44 145.5 45 137.0 46 142.0 47 147.0 48 147.0 49 169.0 50 191.0 51 181.5 52 172.0 53 172.0 54 169.5 55 167.0 56 164.0 57 161.0 58 161.0 59 136.5 60 112.0 61 80.0 62 48.0 63 48.0 64 35.0 65 22.0 66 14.5 67 7.0 68 7.0 69 3.5 70 0.0 71 0.5 72 1.0 73 1.0 74 1.0 75 1.0 76 1.5 77 2.0 78 2.0 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.16189962223421478 8 0.053966540744738264 9 0.0 10 0.0 11 0.053966540744738264 12 0.0 13 0.0 14 0.053966540744738264 15 0.0 16 0.0 17 0.053966540744738264 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.10793308148947653 26 0.37776578521316784 27 0.5396654074473827 28 0.10793308148947653 29 0.21586616297895306 30 0.053966540744738264 31 0.0 32 0.5936319481921208 33 0.0 34 0.0 35 0.0 36 0.32379924446842956 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1853.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.23205612520238 #Duplication Level Percentage of deduplicated Percentage of total 1 94.61722488038278 85.37506745817593 2 3.8875598086124405 7.015650296815974 3 0.6578947368421052 1.7808958445763627 4 0.23923444976076555 0.8634646519158122 5 0.11961722488038277 0.5396654074473827 6 0.0 0.0 7 0.11961722488038277 0.7555315704263357 8 0.05980861244019139 0.4317323259579061 9 0.05980861244019139 0.48569886670264434 >10 0.23923444976076555 2.7522935779816518 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCAACGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 18 0.9713977334052887 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 13 0.7015650296815974 No Hit ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 10 0.5396654074473827 No Hit ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 10 0.5396654074473827 No Hit ATCAACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 9 0.48569886670264434 No Hit ATCAACGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGA 8 0.4317323259579061 No Hit ATCAACGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG 7 0.37776578521316784 No Hit ATCAACGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 7 0.37776578521316784 No Hit ATCAACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC 5 0.26983270372369134 No Hit ATCAACGGGGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA 5 0.26983270372369134 No Hit ATCAACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4 0.21586616297895306 No Hit ATCAACGGGAACTGTGGTAATTCTAGAGCTAATACATGCC 4 0.21586616297895306 No Hit ATCAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4 0.21586616297895306 No Hit ATCAACGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA 4 0.21586616297895306 No Hit ATCAACGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG 3 0.16189962223421478 No Hit ATCAACGGGACTTACTTATTTATATATTTATTTGATTGTT 3 0.16189962223421478 No Hit ATCAACGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG 3 0.16189962223421478 No Hit ATCAACGGGGGATCGGTGATGTTCACAGTTCTCCCTGTGC 3 0.16189962223421478 No Hit ATCAACGGGGGAAAACTTGAGGTTGGAAAATGAATGAAAA 3 0.16189962223421478 No Hit ATCAACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT 3 0.16189962223421478 No Hit ATCAACGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA 3 0.16189962223421478 No Hit ATCAACGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC 3 0.16189962223421478 No Hit ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA 3 0.16189962223421478 No Hit ATCAACGGGGGTTCGATTCCGGAGAGGGAGCCTGAGAAAC 3 0.16189962223421478 No Hit ATCAACGGGATTCGTAGGAGTAAACATAACATTCTTCCCT 3 0.16189962223421478 No Hit ATCAACGGGTGCAGTGCTATCAACGGGGTTGGGCACGGAA 2 0.10793308148947653 No Hit ATCAACGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.10793308148947653 No Hit ATCAACGGGGCTTCTGTAGCTTCTACTGTCCCTGTGGCTA 2 0.10793308148947653 No Hit ATCAACGGGCTCTAAGAGCCATTCTGGGCAAGTGAAGGGC 2 0.10793308148947653 No Hit ATCAACGGGGTCATTTGGCTGCTACTTGCTTAGTGACTCC 2 0.10793308148947653 No Hit ATCAACGGGGTCTTATGTAGTTCAGTCTGGCCTTCAATTC 2 0.10793308148947653 No Hit ATCAACGGGGGGCATGGGATGCTGGGTGGGTGAACTACAC 2 0.10793308148947653 No Hit ATCAACGAGGACTTATGAATGATTGATTTGACAGAGACCC 2 0.10793308148947653 No Hit ATCAACGGGGGTGTGAATGTTGCCGGCGTCTCCCTGAAGT 2 0.10793308148947653 No Hit ATCAACGGGACTCCTATTCTGCCTAGCAAACTCCAACTAC 2 0.10793308148947653 No Hit ATCAACGGGTCAAAGATACTCTTCTGAGATGAGTTTTTGT 2 0.10793308148947653 No Hit ATCAACGGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCC 2 0.10793308148947653 No Hit ATCAACGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGA 2 0.10793308148947653 No Hit ATCAACGGGCTCCTTGTTGAAAAAAAAAAAAAAAAAAAAA 2 0.10793308148947653 No Hit ATCAACGGGGGTTGATCCTGCCAGTAGCATATGCTTGTCT 2 0.10793308148947653 No Hit ATCAACGGGAGTGCAGTGCTATCAACGGGTCACAGATCCT 2 0.10793308148947653 No Hit ATCAACGGGGCTCGCTCCTCTCCTACTTGGATAACTGTGG 2 0.10793308148947653 No Hit ATCAACGGGGGGTACGATTCCGGAGAGGGAGCCTGAGAAA 2 0.10793308148947653 No Hit ATCAACGGGAGATTAGTGTCTAACATGCTAGCTACTCAGT 2 0.10793308148947653 No Hit ATCAACGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGG 2 0.10793308148947653 No Hit ATCAACGGGGCTTTTGCTTGGGATCAGTGCTCTGCTAACG 2 0.10793308148947653 No Hit ATCAACGGGTCACAGATCCTCTTATGAGATGAGTTTTTGT 2 0.10793308148947653 No Hit ATCAACGGGAGCATATGCTTGTCTCAAAGATTAAGCCATG 2 0.10793308148947653 No Hit ATCAACGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAAT 2 0.10793308148947653 No Hit ATCAACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTT 2 0.10793308148947653 No Hit ATCAACGGGGGGGTTTAATGAGGTGACCTGTAGCTTTGGC 2 0.10793308148947653 No Hit ATCAACGGGGTTTGTTTTATTTTGTTCTAAAATTAAAAGT 2 0.10793308148947653 No Hit ATCAACGGGTCACAAATCCTCTTCTGAAGGAGATTGGGCG 2 0.10793308148947653 No Hit ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAAAG 2 0.10793308148947653 No Hit ATCAACGGGGAAAGTTTTATTTATAATATGCATTTTTTTT 2 0.10793308148947653 No Hit ATCAACGGGGAAAGAGATTATACTTTCTACATTTGGCAGG 2 0.10793308148947653 No Hit ATCAACGGGATCTTGGGGCAAAAGAAAACAAAAGCAGAAA 2 0.10793308148947653 No Hit ATCAACGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT 2 0.10793308148947653 No Hit ATCAACGGGAGGGGGAAGAGTGGGAAAACCTTTCAAGGAA 2 0.10793308148947653 No Hit ATCAACGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGC 2 0.10793308148947653 No Hit ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG 2 0.10793308148947653 No Hit ATCAACGGGGGGTTCGATTCCGGAGAGGGAGCCTAAGAAA 2 0.10793308148947653 No Hit ATCAACGGGATGTAGTTACTTACTACTCCGGAAAAAAAGA 2 0.10793308148947653 No Hit ATCAACGGGGAAAGCGTAGGGCTTGGGGGTTGGTCTGGTC 2 0.10793308148947653 No Hit ATCAACGGGGGTTTGGTTTTTGTTTTTGTTTTTCCTTTAA 2 0.10793308148947653 No Hit ATCAACGGGGGGGTTGGGAATTTAGCTCAGTGGTAGAACG 2 0.10793308148947653 No Hit ATCAACGGGGTCTAAGTACGCACGGCCGGTACAGTGAAAC 2 0.10793308148947653 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGC 2 0.10793308148947653 No Hit ATCAACGGGAGGCGCGCAAATTACCCACTCCCGACCCGGG 2 0.10793308148947653 No Hit ATCAACGGGAGGCCCTGTAATTGGAATGAGTCCACTTTAA 2 0.10793308148947653 No Hit ATCAACGGGGGGAGCAGTGTTTGCTATCATAGCAGGATTT 2 0.10793308148947653 No Hit ATCAACGGGGAAATCCTGATTCCAGGACTCAGGCCAGCCT 2 0.10793308148947653 No Hit ATCAACGGGGCTTATCCTTCTGTATCACCAATACCATCGC 2 0.10793308148947653 No Hit ATCAACGGGGTCTCTTCTATAGGATCATTTATTTCACTAA 2 0.10793308148947653 No Hit ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAAAA 2 0.10793308148947653 No Hit ATCAACGGGACCCACTCCCGACCCGGGGAGGTAGTGACGA 2 0.10793308148947653 No Hit ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG 2 0.10793308148947653 No Hit ATCAACGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC 2 0.10793308148947653 No Hit ATCAACGGGATGGGAAGCCCTTACAGAGGGGCTTCTCCAA 2 0.10793308148947653 No Hit ATCAACGGGCATCCAGATGACCACGACACTCGGTGAACAT 2 0.10793308148947653 No Hit ATCAACGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTC 2 0.10793308148947653 No Hit ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 2 0.10793308148947653 No Hit ATCAACGGGACTGTTTATTTGCAGATGTTTGACAAAGGGA 2 0.10793308148947653 No Hit ATCAACGGGTGAGAGGAATGATAGCAACCTGCCTCTATTT 2 0.10793308148947653 No Hit ATCAACGGGTCACAGATCCTCTTCTGAAGGAGATTGGGAG 2 0.10793308148947653 No Hit ATCAACGGGATGGTTGACATTTTGCAGAATAACTCTAATG 2 0.10793308148947653 No Hit ATCAACGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT 2 0.10793308148947653 No Hit ATCAACGGGATAGCAATAATAGCTCTACTAAACCTATTCT 2 0.10793308148947653 No Hit ATCAACGGGGGCTTTGGTGAATCTAGATAACCTCGGGCCG 2 0.10793308148947653 No Hit ATCAACGGGATGTTATAGCTTAACAGAATTACTAATTTCG 2 0.10793308148947653 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.053966540744738264 0.0 0.0 0.0 0.0 16 0.053966540744738264 0.0 0.0 0.0 0.0 17 0.10793308148947653 0.0 0.0 0.0 0.0 18 0.10793308148947653 0.0 0.0 0.0 0.0 19 0.10793308148947653 0.0 0.0 0.0 0.0 20 0.10793308148947653 0.0 0.0 0.0 0.0 21 0.10793308148947653 0.0 0.0 0.0 0.0 22 0.10793308148947653 0.0 0.0 0.0 0.0 23 0.10793308148947653 0.0 0.0 0.0 0.0 24 0.10793308148947653 0.0 0.0 0.0 0.0 25 0.10793308148947653 0.0 0.0 0.0 0.0 26 0.10793308148947653 0.0 0.0 0.0 0.0 27 0.10793308148947653 0.0 0.0 0.0 0.0 28 0.10793308148947653 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGGGG 20 0.0021324127 34.0 6 ACGGGTA 20 0.0021324127 34.0 5 ACGGGGG 25 1.3509655E-4 34.0 5 ACGGGAT 20 0.0021324127 34.0 5 AACGGGG 80 0.0 34.0 4 AACGGGC 20 0.0021324127 34.0 4 AACGGGA 45 1.6807462E-9 34.0 4 TCAACGG 195 0.0 32.25641 2 ATCAACG 195 0.0 32.25641 1 CAACGGG 195 0.0 32.25641 3 ACGGGGA 40 1.4356501E-6 29.75 5 AACGGGT 50 2.113029E-7 27.199999 4 >>END_MODULE