FastQCFastQC Report
Fri 17 Jun 2016
SRR1525806_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525806_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72353
Sequences flagged as poor quality0
Sequence length56
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG4820.6661783201802275No Hit
GCCCTCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC2530.349674512459746No Hit
GCCCTCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT2380.3289428219977057No Hit
GCCCTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT2150.2971542299559106No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2010.27780465219133965No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1900.26260141251917685No Hit
GCCCTCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC1710.23634127126725912No Hit
GCCCTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC1410.19487789034317857No Hit
GCCCTCGGGCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAA1310.18105676336848506No Hit
GCCCTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1180.1630892983013835No Hit
GCCCTCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC1170.16170718560391414No Hit
GCCCTCGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC1150.15894296020897544No Hit
GCCCTCGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT1090.15065028402415934No Hit
GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1050.14512183323428193No Hit
GCCCTCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACCACCCATTCGAACGTC1040.14373972053681258No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT910.125772255469711No Hit
GCCCTCGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC910.125772255469711No Hit
GCCCTCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA910.125772255469711No Hit
GCCCTCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC890.12300803007477229No Hit
GCCCTCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC850.1174795792848949No Hit
GCCCTCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA840.11609746658742554No Hit
GCCCTCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG790.10918690310007878No Hit
GCCCTCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT730.10089422691526267No Hit
GCCCTCGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT730.10089422691526267No Hit
GCCCTCGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCG730.10089422691526267No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACCC150.00817998150.335239
ACCGCTA150.00817998150.335239
ACCGCGA150.00817998150.335239
CACGATT150.00817998150.335247
TCGGACT150.00817998150.335247
CACTATC150.00817998150.335247
AGCGCGA150.00817998150.335247
TCCACGA150.00817998150.335245
ACTCGAA150.00817998150.335246
CCACGAT150.00817998150.335246
ACCTAAT251.9875664E-550.33519740
CGGACAG150.00824813850.2297537
CGTCTCG150.00824813850.2297542
ATTATAC150.00824813850.2297542
TAGACCT252.0124193E-550.22974837
AAACCGC204.0644742E-450.22974837
CTAGACC301.006707E-650.194736
GGTAAGC150.0082709550.194734
GAGCGTC150.0082709550.194734
CCGAAAA507.2759576E-1250.194736