##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525806_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72353 Sequences flagged as poor quality 0 Sequence length 56 %GC 59 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.414993158542146 39.0 38.0 39.0 35.0 40.0 2 37.19190634804362 39.0 38.0 39.0 33.0 40.0 3 37.619725512418285 39.0 38.0 39.0 35.0 40.0 4 37.34125744613216 39.0 38.0 39.0 33.0 40.0 5 37.10481942697608 39.0 37.0 39.0 33.0 40.0 6 36.879078960098404 39.0 37.0 39.0 33.0 40.0 7 36.695796995287 38.0 36.0 39.0 32.0 40.0 8 36.715257142067365 39.0 36.0 39.0 31.0 40.0 9 36.84044891020414 39.0 37.0 39.0 32.0 40.0 10 36.542990615454784 39.0 37.0 39.0 32.0 40.0 11 35.36715823808273 38.0 35.0 39.0 29.0 40.0 12 34.11395519190635 38.0 33.0 39.0 23.0 40.0 13 33.57523530468674 38.0 32.0 39.0 21.0 40.0 14 34.081309689992125 38.0 33.0 39.0 23.0 40.0 15 33.16228767293685 38.0 32.0 39.0 18.0 40.0 16 32.47423050875568 36.0 30.0 38.0 19.0 40.0 17 31.55484914239907 36.0 28.0 38.0 15.0 39.0 18 30.907135847857035 35.0 27.0 38.0 14.0 39.0 19 31.950561828811523 36.0 29.0 38.0 18.0 39.0 20 32.570605227150224 36.0 30.0 38.0 20.0 40.0 21 32.48400204552679 36.0 30.0 39.0 18.0 40.0 22 31.823711525437783 36.0 29.0 39.0 15.0 40.0 23 32.431592332038754 36.0 30.0 39.0 18.0 40.0 24 31.8421074454411 36.0 29.0 38.0 16.0 40.0 25 32.228767293685124 36.0 30.0 38.0 18.0 40.0 26 32.66970270755878 37.0 31.0 39.0 18.0 40.0 27 32.93005127638108 37.0 31.0 39.0 19.0 40.0 28 32.90286511962185 37.0 31.0 39.0 20.0 40.0 29 32.37905822840794 36.0 31.0 38.0 17.0 39.0 30 32.149724268516856 36.0 30.0 38.0 17.0 39.0 31 32.922850469227264 37.0 32.0 39.0 19.0 39.0 32 32.7462026453637 37.0 32.0 38.0 18.0 39.0 33 32.55703288046107 36.0 31.0 38.0 18.0 39.0 34 32.37751026218678 36.0 31.0 38.0 17.0 39.0 35 32.213536411759016 36.0 31.0 38.0 17.0 39.0 36 31.865741572567828 36.0 31.0 38.0 11.0 39.0 37 31.523350794023745 36.0 30.0 38.0 10.0 39.0 38 30.846502563819055 35.0 29.0 38.0 4.0 39.0 39 29.865976531726396 35.0 27.0 38.0 4.0 39.0 40 28.034815418849252 33.0 20.0 38.0 4.0 39.0 41 26.429035423548434 32.0 17.0 37.0 4.0 39.0 42 26.982986192694153 32.0 19.0 37.0 4.0 39.0 43 26.964203281135543 32.0 19.0 38.0 4.0 39.0 44 26.03109753569306 31.0 16.0 37.0 4.0 39.0 45 26.810871698478294 32.0 19.0 37.0 4.0 39.0 46 24.74743272566445 30.0 12.0 36.0 4.0 39.0 47 23.77446685002695 28.0 9.0 35.0 4.0 38.0 48 24.352383453346786 29.0 10.0 36.0 4.0 38.0 49 23.281232291681064 28.0 5.0 35.0 4.0 38.0 50 23.886528547537765 29.0 4.0 35.0 4.0 38.0 51 24.03752435973629 30.0 4.0 36.0 4.0 38.0 52 23.78371318397302 30.0 4.0 36.0 4.0 38.0 53 23.638384033834118 30.0 4.0 36.0 4.0 38.0 54 23.232457534587372 29.0 4.0 35.0 4.0 38.0 55 22.87855375727337 29.0 4.0 35.0 4.0 38.0 56 22.209542106063328 28.0 4.0 35.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.4994526105960375 1 2 1.7394507347372254 1 3 1.0259837625566917 1 4 1.6862807150611658 1 5 1.8547149613617222 1 6 2.3603870507029185 1 7 2.0177843003045624 1 8 2.421344111681705 1 9 2.2793462656161765 1 10 2.1218654753016324 1 11 -0.35257921856501895 1 12 -8.089879775555612 1 13 -19.674851171303054 1 14 -7.9604791033076765 1 15 0.7009683594026157 1 16 -0.9614955726855534 1 17 -5.611990212208006 1 18 -4.169135395584391 1 19 0.5570387033585575 1 20 -0.8902447331744447 1 21 5.608558891269986 1 22 7.168367497838869 1 23 5.879978312674268 1 24 6.721195605818071 1 25 5.90939072899323 1 26 5.662805025366108 1 27 5.410670050412527 1 28 5.685015543495005 1 29 6.118236368733186 1 30 5.567481837496789 1 31 4.385817111266153 1 32 4.357347538349401 1 33 4.910269246623152 1 34 4.81931570395669 1 35 3.8436099807130546 1 36 3.81192646146712 1 37 2.873344460754037 1 38 3.9815338002150007 1 39 4.645920542689822 1 40 -9.602322586508746 1 41 -0.6841238522211448 1 42 -1.4629340173193164 1 43 -8.222222261882509 1 44 -14.45688339104709 1 45 -2.6614677190765867 1 46 -5.409619091754621 1 47 -9.820033023949602 1 48 -4.919830027600774 1 49 -12.669358906179589 1 50 -3.79405933418564 1 51 1.8538570332017983 1 52 1.416516308974117 1 53 2.1248817791186987 1 54 2.329720299818362 1 55 1.8606840199115418 1 56 5.297106932205104 2 1 1.8689812301246604 2 2 2.167136685150446 2 3 1.5432779162144854 2 4 2.1956164971242202 2 5 1.9385839543216221 2 6 2.800010558508248 2 7 2.7758957175068915 2 8 2.687796514497748 2 9 2.7742192377618764 2 10 3.042970464588443 2 11 3.1636436158396393 2 12 0.22899074786036522 2 13 1.2350814886296106 2 14 4.410273881081672 2 15 4.742902855882569 2 16 5.048988051434431 2 17 6.798707675762611 2 18 5.043980612986164 2 19 6.3890711490273695 2 20 5.985635279069204 2 21 7.2017636662929405 2 22 8.23471276872959 2 23 7.382886179218502 2 24 7.92551149195214 2 25 6.443749467897419 2 26 6.100515463076551 2 27 6.217985630455374 2 28 5.8978254608503775 2 29 6.87129728088501 2 30 6.419257166544849 2 31 5.850922712735386 2 32 5.240344171346038 2 33 5.267860308759666 2 34 4.658694338789871 2 35 4.690640895925789 2 36 4.960610269241837 2 37 5.0165186266554755 2 38 5.97686590918347 2 39 6.802027062432707 2 40 3.8985191643330026 2 41 8.832252027791103 2 42 7.358691327033302 2 43 5.928910275613664 2 44 -4.113372524808952 2 45 6.611489122062071 2 46 6.074158073840728 2 47 3.9636370097204328 2 48 6.754335873837853 2 49 -0.7582784041899941 2 50 6.397247675303188 2 51 9.921273623345662 2 52 8.492733021554468 2 53 8.839298675353778 2 54 9.513604597339022 2 55 10.023677591996023 2 56 10.408906748550372 3 1 2.0251395878959215 3 2 2.0459023476404496 3 3 1.5907985773715083 3 4 2.104440810640618 3 5 1.9697089876460723 3 6 2.8505304198785524 3 7 2.691918111535152 3 8 2.875346501167968 3 9 2.818474103131109 3 10 2.593490325958747 3 11 4.124720661960673 3 12 4.78377613842288 3 13 4.85531609273518 3 14 5.406008351889454 3 15 1.1334653725447907 3 16 6.598241583825793 3 17 7.676540253741816 3 18 7.950338917438312 3 19 7.374541690216386 3 20 6.235501981281946 3 21 7.907842045391845 3 22 8.483779684218792 3 23 7.531113318647982 3 24 8.462235032341372 3 25 7.626833978694542 3 26 7.365911357504704 3 27 6.8306222606873135 3 28 6.523725220914358 3 29 7.272656918315029 3 30 7.357207046576843 3 31 6.080261555710596 3 32 5.801194611228738 3 33 6.695812854747402 3 34 6.028395751746416 3 35 6.507289789554157 3 36 6.991735302566287 3 37 6.975196312605888 3 38 7.611163429844627 3 39 8.908465345557204 3 40 9.266554354948838 3 41 12.062590604170733 3 42 11.187603617089287 3 43 11.284348825333737 3 44 12.340906334161993 3 45 10.831363822142048 3 46 13.016306834171306 3 47 13.128593021450634 3 48 13.149763759734949 3 49 14.115288644596994 3 50 13.216095982421287 3 51 12.893880928064402 3 52 12.907555735497418 3 53 12.832767083778194 3 54 13.450688041753843 3 55 12.642046027080003 3 56 11.354454602455998 4 1 1.7837036758205542 4 2 1.949627725887666 4 3 1.4956658340086761 4 4 2.1349860575718154 4 5 0.5849673376317952 4 6 2.5489317901326203 4 7 2.03933461167788 4 8 2.6434024532090703 4 9 2.261237448834791 4 10 2.004281076743787 4 11 3.7082205192258684 4 12 3.891683646273293 4 13 4.482064594019789 4 14 4.443404869160368 4 15 4.641315786475708 4 16 6.972706328330506 4 17 7.713722668814608 4 18 7.966967521491977 4 19 7.269346143542105 4 20 6.2043144277563975 4 21 6.831764397661331 4 22 7.340688048478004 4 23 6.751495847908622 4 24 6.980290984382474 4 25 6.282629011523547 4 26 5.878472019794163 4 27 5.90313153844923 4 28 5.266873950574649 4 29 5.525725716488019 4 30 6.205551322911408 4 31 4.949730582259267 4 32 4.855790671748288 4 33 5.030311855603813 4 34 5.134376339813791 4 35 5.271420534629804 4 36 5.483099847242276 4 37 5.019444100216511 4 38 5.091251925419851 4 39 5.602656039248323 4 40 -0.6799200955221849 4 41 9.167072476851292 4 42 9.298865392710184 4 43 8.803261186167312 4 44 10.09711529790735 4 45 8.85598557492538 4 46 11.503032497888118 4 47 11.250845376574816 4 48 11.219632443720105 4 49 12.119071116763706 4 50 12.23707799783665 4 51 12.04567938652858 4 52 11.74198336895445 4 53 11.713868996424502 4 54 11.314233574154645 4 55 11.960487363077721 4 56 10.827834562490253 5 1 1.7967409703843984 5 2 1.5340935918800795 5 3 1.1622271488000777 5 4 1.8288071700876216 5 5 1.2637758079225705 5 6 1.9804267332426022 5 7 2.0994641944844545 5 8 2.243434058771655 5 9 2.1540155777854864 5 10 2.473386639322797 5 11 3.525066284080488 5 12 3.939882129206289 5 13 4.791087982136098 5 14 4.665512960184387 5 15 6.213534496968755 5 16 6.447895961705978 5 17 7.2312637560459585 5 18 8.596076244627252 5 19 6.696258280078137 5 20 6.557506589576878 5 21 6.776217621428714 5 22 7.452097656569027 5 23 6.792346037541996 5 24 7.401751161373628 5 25 6.996361628464129 5 26 5.87973624229733 5 27 4.638513965756438 5 28 4.890372686352151 5 29 5.609638485135299 5 30 5.464637922152878 5 31 4.544547269996311 5 32 3.9132337817356486 5 33 4.257160781014683 5 34 4.439686074327064 5 35 4.397842784945858 5 36 4.875087837128493 5 37 5.403767741177319 5 38 5.9772348584160575 5 39 7.127071336340613 5 40 8.292187995501834 5 41 10.68327032767304 5 42 10.194804077918782 5 43 10.56084652118627 5 44 10.717058407894712 5 45 9.613333868225002 5 46 10.62344969131416 5 47 11.853247399330819 5 48 11.619521824251077 5 49 12.438445326624645 5 50 10.927501512375208 5 51 11.101542218386985 5 52 10.784571864529674 5 53 11.405635747372664 5 54 10.87436315696122 5 55 10.987668146895672 5 56 10.830837090935262 6 1 1.8191788585831574 6 2 1.7677116043024412 6 3 1.3973203165019399 6 4 1.9748475427584253 6 5 1.9497230128915106 6 6 2.5171503357270737 6 7 2.452647424298121 6 8 2.735514804113265 6 9 2.4787826585308324 6 10 2.6209798070246606 6 11 3.9606252902916665 6 12 5.119540514299459 6 13 5.271445124993242 6 14 5.141211717948366 6 15 5.448472385167513 6 16 6.864510868538279 6 17 7.592599159772671 6 18 7.706790530341539 6 19 6.968230329767575 6 20 6.646792303862593 6 21 6.913872900931821 6 22 6.7671597683702664 6 23 6.724100695927085 6 24 6.8897946396344985 6 25 6.709793305482762 6 26 5.2586182298749655 6 27 5.201169245259543 6 28 4.939363369581962 6 29 6.510104323644619 6 30 5.344373946997596 6 31 4.617218077449724 6 32 4.769988440120741 6 33 5.423232209586111 6 34 4.739686074327061 6 35 4.477345890535922 6 36 5.250010197376941 6 37 5.470848486518932 6 38 6.295946038540276 6 39 6.45075146056422 6 40 3.939625883700593 6 41 10.718130576120242 6 42 9.866776127608219 6 43 10.084138446652112 6 44 10.854791327149371 6 45 9.667759334063508 6 46 11.790219877649566 6 47 12.355731871380513 6 48 12.023326172077166 6 49 12.279594394947623 6 50 12.24007915212676 6 51 11.945563957517418 6 52 11.119897951486195 6 53 11.635992890229808 6 54 11.692763778079232 6 55 12.090152618945357 6 56 11.048073115779982 7 1 2.145956591212169 7 2 2.0315068095035826 7 3 1.5510789512032872 7 4 2.3055261286991 7 5 2.100474739831782 7 6 2.8476663861130973 7 7 2.5291204027621603 7 8 2.7669654339385445 7 9 3.045787940936357 7 10 2.4924954510717967 7 11 4.2869154162567185 7 12 5.026163838151554 7 13 5.656491853965207 7 14 5.1841211043766435 7 15 6.085530491628305 7 16 6.9944580444830144 7 17 7.805195665258243 7 18 8.338566231276115 7 19 7.520607412254368 7 20 6.291652116946704 7 21 7.193190251602282 7 22 8.27402689555434 7 23 6.8678633940172915 7 24 7.748713778196059 7 25 7.101585259972808 7 26 6.652766949907477 7 27 6.101047343593557 7 28 5.780809936018372 7 29 6.585439553226088 7 30 6.533646600390529 7 31 4.806897069729285 7 32 4.936181044753006 7 33 5.492391088090784 7 34 5.846228130401833 7 35 5.785025695493253 7 36 6.019697316434236 7 37 6.732205658400282 7 38 7.365836327040892 7 39 8.288703512575594 7 40 7.453319504180072 7 41 10.89496925958225 7 42 10.355601808225856 7 43 9.841554131333133 7 44 10.504608474650382 7 45 10.285313173965985 7 46 12.203222722021774 7 47 12.295837519491037 7 48 11.918003132662289 7 49 12.15289793009594 7 50 11.367588294751705 7 51 11.77035062960194 7 52 11.355249434623133 7 53 11.843469525743828 7 54 11.785327776453876 7 55 11.121847052102613 7 56 9.902766329920574 8 1 2.1862717827093263 8 2 2.097293871992129 8 3 1.6251123028617016 8 4 2.2211391028607324 8 5 1.93096768467327 8 6 2.809733455931678 8 7 2.3538409451847357 8 8 2.2535881203963015 8 9 3.005381124003975 8 10 2.9742619894628533 8 11 4.280318384920825 8 12 5.620904538169874 8 13 5.694974536757947 8 14 5.008475145058334 8 15 5.523408446548586 8 16 7.0749968020421115 8 17 7.876571879295259 8 18 8.71267288328271 8 19 7.504206459349842 8 20 6.970321715627296 8 21 6.700062159243842 8 22 7.843204099146053 8 23 6.88215696191174 8 24 7.266947239805003 8 25 6.923007286727433 8 26 6.364330079832339 8 27 5.863829091806856 8 28 4.650701818004812 8 29 6.235338765247349 8 30 6.070205149043634 8 31 4.545947830220403 8 32 5.333927059046573 8 33 5.505585150762585 8 34 5.263215486091767 8 35 6.112128499231574 8 36 5.805935176064072 8 37 6.896764940056869 8 38 7.3709673514132525 8 39 8.074025117853218 8 40 4.081228611748333 8 41 10.921540635796287 8 42 9.68902676699441 8 43 10.15958601698459 8 44 10.35956541069583 8 45 9.52317280379923 8 46 11.3006205596615 8 47 11.526916867117933 8 48 11.941825745819703 8 49 12.231512553515401 8 50 11.213565938145514 8 51 9.84786574779875 8 52 10.638738531196342 8 53 10.588934066700396 8 54 9.78560265275954 8 55 10.161337614682786 8 56 9.864100396257388 9 1 2.2010777050782764 9 2 2.19353236739029 9 3 1.7081455161470203 9 4 2.3249806394753776 9 5 1.8441329507797093 9 6 2.6908859169160735 9 7 2.508902969994665 9 8 2.8378218138736955 9 9 2.9843727206426323 9 10 2.260376435241163 9 11 3.7929234269376266 9 12 5.054678047573638 9 13 5.106139002544268 9 14 4.577502756102753 9 15 5.450779394927942 9 16 7.200446982114141 9 17 8.167488245841877 9 18 7.945566040545618 9 19 7.23069705558834 9 20 6.636588222229943 9 21 6.655044152040958 9 22 7.342403779018003 9 23 6.747703180399135 9 24 7.531853202189954 9 25 5.701718771321268 9 26 6.338919915766713 9 27 6.081116006572763 9 28 5.363586972066436 9 29 5.494842566767957 9 30 6.075607309907976 9 31 5.299649310812637 9 32 4.962978679694828 9 33 4.980375066728968 9 34 4.970298319225023 9 35 5.826414213517289 9 36 5.121261306516249 9 37 5.2813187615854815 9 38 6.2897348584160575 9 39 6.613040724095718 9 40 3.2194839138691833 9 41 8.138627470530178 9 42 9.535365302408579 9 43 9.18839754159444 9 44 9.551242081364094 9 45 8.930935909041327 9 46 11.842837446416201 9 47 12.026716787085924 9 48 11.646307538536792 9 49 11.614465734787906 9 50 11.07290967564051 9 51 10.156389157162497 9 52 11.033296354325593 9 53 10.746196971862464 9 54 11.447067238593874 9 55 10.88562733056914 9 56 10.29767382562914 10 1 1.5017445064522903 10 2 1.5587576654302921 10 3 1.4249569932130939 10 4 2.0606848221385405 10 5 1.6871315363525525 10 6 2.7858545974576003 10 7 1.9780886640742708 10 8 2.192337877724974 10 9 2.4681068083371116 10 10 2.4050521273001664 10 11 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1.3996241955279345 114 5 1.3529392130106288 114 6 2.029717339896223 114 7 1.8390679126840652 114 8 1.4946571507481679 114 9 1.6947445406034944 114 10 1.3274717665243614 114 11 1.3173852664679586 114 12 -3.8627108257839247 114 13 -5.840883642130045 114 14 -5.227221873475102 114 15 -6.214303076416762 114 16 -1.4540120617773873 114 17 2.064189392053791 114 18 1.4656946399305788 114 19 1.688063563835474 114 20 1.386518331259854 114 21 5.011907445303471 114 22 4.6984283806394735 114 23 5.628329403491115 114 24 4.913737462743494 114 25 5.577452626507181 114 26 4.627290058344297 114 27 3.7183818718229773 114 28 4.982960748974456 114 29 4.634344943060938 114 30 3.694701522935656 114 31 3.7275218296832016 114 32 3.8742171476847673 114 33 4.664328099997071 114 34 3.720507992135282 114 35 3.9888408279987004 114 36 4.525650459437696 114 37 5.482045627674381 114 38 5.2721223339542185 114 39 6.128416737514783 114 40 7.704247682390289 114 41 8.188529623172059 114 42 7.803047717840503 114 43 7.39205982842698 114 44 0.07444588343286895 114 45 6.512428780162377 114 46 1.7149369711576092 114 47 -1.6291400762073387 114 48 2.034761550278475 114 49 -5.952210250674769 114 50 2.361699164038612 114 51 4.227398382770549 114 52 3.7802910426118643 114 53 3.7428422052983024 114 54 3.497528518996443 114 55 3.4641847809465496 114 56 6.518948636923518 115 1 1.7182635267753739 115 2 1.7394507347372254 115 3 1.248693314213611 115 4 1.7559688242229612 115 5 1.650523928223322 115 6 2.537287635498245 115 7 2.3048213373416004 115 8 2.3345994723054844 115 9 2.441139637935862 115 10 2.1355106994731727 115 11 1.3925474870880024 115 12 -0.7960201264328077 115 13 -2.694344348690965 115 14 -5.774806588687792 115 15 -4.539285051903423 115 16 2.4751516008037235 115 17 2.880699846271522 115 18 3.467316846131009 115 19 2.1788710620330214 115 20 2.1994238828099633 115 21 5.329319125188114 115 22 6.310180363335942 115 23 5.347327240549511 115 24 6.066224845584156 115 25 5.607733808915256 115 26 5.0526685731243965 115 27 5.066615469515838 115 28 5.185015543495005 115 29 5.035390364834548 115 30 5.47732589207769 115 31 4.803945766236911 115 32 4.843214984743163 115 33 4.136390104322956 115 34 4.597580811169166 115 35 3.5331811308105188 115 36 4.540971295775112 115 37 4.764669996816416 115 38 5.308531850897257 115 39 6.394458554385729 115 40 7.549236867682289 115 41 8.655544763763263 115 42 8.427416859873667 115 43 7.032651032464468 115 44 1.2029606635338084 115 45 4.796134620104699 115 46 0.9242015710134126 115 47 -7.626075908939855 115 48 1.9978112979352858 115 49 -5.203471966608438 115 50 1.6859601589917474 115 51 3.4995685341764577 115 52 3.4194402855823043 115 53 4.091256048124546 115 54 3.1191939840288896 115 55 3.4030816807302564 115 56 6.123130324017968 116 1 1.8628525273667407 116 2 2.141136127995651 116 3 1.5711356560231948 116 4 1.9888192086108916 116 5 1.7831377661890215 116 6 2.5136329932747046 116 7 2.4845966182404737 116 8 2.7881772369417988 116 9 2.4723341973681556 116 10 2.2060311016020933 116 11 3.4799218218011916 116 12 3.2350265914855854 116 13 4.0062765856674005 116 14 3.859433505930774 116 15 -2.368528103352272 116 16 4.189669637468423 116 17 5.479457977554791 116 18 6.057352328094343 116 19 5.477658761619065 116 20 4.523196798244616 116 21 6.176598841332407 116 22 6.941463627676573 116 23 5.872903822453715 116 24 6.675627565868009 116 25 6.9400815321559435 116 26 6.175081346631199 116 27 4.956030017120803 116 28 4.7496222850680425 116 29 6.273563043722781 116 30 6.0754926573053645 116 31 4.8358795332386855 116 32 5.287330892490061 116 33 5.061434456777128 116 34 5.096989445113579 116 35 5.190780184624835 116 36 5.56920902492946 116 37 6.098791818223866 116 38 6.72633196917047 116 39 6.767019169406424 116 40 8.140602923270698 116 41 8.970890712905781 116 42 7.459350626875441 116 43 2.9199957988748757 116 44 2.8432622602221578 116 45 5.740841893907181 116 46 0.5781045869802952 116 47 -6.083349711423594 116 48 0.8477521613297299 116 49 -3.0553347696835402 116 50 0.4714421223270513 116 51 4.654210757712828 116 52 4.342858381383607 116 53 4.618015362139921 116 54 4.770731535773418 116 55 3.9836553058041773 116 56 5.76121429799786 117 1 0.990193351336778 117 2 1.1561174014038897 117 3 0.5241319107048383 117 4 1.1436881224685749 117 5 1.2417519983987546 117 6 1.6548314951473628 117 7 1.8381546706749319 117 8 2.0398626302002256 117 9 1.7200870063569198 117 10 1.7811247345608976 117 11 0.6881615221757258 117 12 -8.089879775555612 117 13 -8.711888208340088 117 14 -1.432701325529898 117 15 -5.680513122078864 117 16 -7.481865943055926 117 17 0.1991208989031037 117 18 -2.9098761363251313 117 19 0.42185351817337136 117 20 -0.8698743628040724 117 21 4.869670002381099 117 22 5.046145275616642 117 23 3.84294127563723 117 24 4.12675116137363 117 25 4.027909247511744 117 26 4.720212432773518 117 27 4.456966346708818 117 28 4.8016822101616725 117 29 4.910828961325777 117 30 4.371185541200493 117 31 3.130261555710593 117 32 3.1554956864975523 117 33 3.8898988762527793 117 34 3.8896860743270665 117 35 3.0454618325649037 117 36 4.797111646652308 117 37 5.058529645939224 117 38 5.177830096511297 117 39 6.518142764912042 117 40 8.327307043120882 117 41 7.019579851482561 117 42 5.287065982680684 117 43 -5.046296335956583 117 44 -0.8865130206767198 117 45 5.449643392034524 117 46 0.8496401675046386 117 47 -1.0515145054310828 117 48 2.06535515758441 117 49 -0.5508403876610686 117 50 3.0263110361847296 117 51 6.205708885053653 117 52 6.638738531196342 117 53 6.985992890229809 117 54 8.379720299818363 117 55 7.432906242133765 117 56 10.058218043316213 118 1 0.8871630483064763 118 2 1.0894507347372269 118 3 -0.050110513537582335 118 4 1.0997487285291783 118 5 1.0856913923381484 118 6 1.6033163436322155 118 7 1.6320940646143285 118 8 1.3671353574729537 118 9 1.5064506427205515 118 10 1.573548976985137 118 11 -0.48759605358185354 118 12 -8.979273714949553 118 13 -9.29219123864312 118 14 -10.12361041643899 118 15 -9.410816152381894 118 16 -9.703078064268045 118 17 -2.196333646551441 118 18 -6.093209469658465 118 19 -2.36299496667511 118 20 -2.371389514319226 118 21 3.234821517532609 118 22 4.590084669556038 118 23 3.994456427152386 118 24 3.9040238886463534 118 25 4.090030459632956 118 26 5.100515463076551 118 27 4.122117861860332 118 28 4.362288270767735 118 29 4.454768355265173 118 30 4.878761298776251 118 31 3.8575342829833197 118 32 3.458525989527857 118 33 3.3141413004952014 118 34 4.376049710690701 118 35 4.9666739537770255 118 36 4.744081343622003 118 37 4.138832676242252 118 38 6.0975270662082615 118 39 7.0681427649120465 118 40 9.116700982514821 118 41 6.2256404575431645 118 42 6.044641740256441 118 43 -0.1735690632293121 118 44 -0.009240293403991018 118 45 5.542067634458764 118 46 0.5072159250803985 118 47 1.0727279188113386 118 48 0.922930915160169 118 49 1.25825052142984 118 50 3.8732807331544272 118 51 5.84358767293244 118 52 5.226617319075128 118 53 3.799629253866172 118 54 6.2706293907274535 118 55 5.952603211830734 118 56 6.109733194831364 119 1 0.7499972729054107 119 2 0.8570977935607544 119 3 0.26726916560680536 119 4 0.7799626322724933 119 5 0.6907716062418956 119 6 1.6773805147552068 119 7 1.4518801608710135 119 8 1.3810391007884562 119 9 1.520087006356917 119 10 1.4350463031883436 119 11 9.066202149412561E-4 119 12 -11.300664089281103 119 13 -4.878554875006756 119 14 -6.19740720788284 119 15 -6.5648268475690585 119 16 -9.380885550899063 119 17 -0.6626438069792471 119 18 -8.404974175540815 119 19 -1.1330484426109422 119 20 -1.0737959314315226 119 21 4.479473923949726 119 22 4.245164883459783 119 23 4.13215696191174 119 24 3.71792763196186 119 25 3.8445759141784137 119 26 4.192761452381358 119 27 3.6726526212186243 119 28 3.7732508376126503 119 29 4.303966216227742 119 30 4.555499266690692 119 31 4.344967438063538 119 32 3.054515294340689 119 33 4.020291033115527 119 34 4.23380372138589 119 35 3.3474226168786316 119 36 3.5804449799856393 119 37 3.945784547900004 119 38 4.302339900432859 119 39 5.33873100020616 119 40 6.958679592140491 119 41 4.499972008345306 119 42 3.2037326493473515 119 43 -4.311002218309525 119 44 -1.1453365500884836 119 45 2.165329666544327 119 46 -2.2189872834757516 119 47 -2.3652399956271637 119 48 -5.39150758751363 119 49 -2.8420168582493055 119 50 -0.2080026893054665 119 51 4.686101041916398 119 52 4.736777746882616 119 53 4.721287007876867 119 54 4.109132064524246 119 55 5.195651340172983 119 56 6.447433729590724 120 1 0.8972971764733941 120 2 1.2271556527700156 120 3 0.5457166101583937 120 4 1.1647263738346965 120 5 0.9354678453932905 120 6 1.7296948831255108 120 7 1.7802311734071736 120 8 1.9480593515117022 120 9 1.3356607768487194 120 10 1.3824908547794763 120 11 -0.7785051444909428 120 12 -11.408185786484577 120 13 -6.374456514351017 120 14 -6.3671275550380955 120 15 -1.8406224116963479 120 16 -8.920663757263576 120 17 -0.07055123224443705 120 18 -6.837471764740432 120 19 -0.8562885583293607 120 20 -0.5827158928587188 120 21 4.233331204566888 120 22 4.961172598021019 120 23 5.307422149954171 120 24 5.694783948258877 120 25 4.229821815817758 120 26 6.097534837145101 120 27 5.753414434140517 120 28 5.648130297593369 120 29 5.832413660779331 120 30 5.778016142293392 120 31 4.838458277022074 120 32 4.4877361236560205 120 33 5.049461717782833 120 34 5.398702467769688 120 35 5.177702269723376 120 36 6.432903996379078 120 37 5.958802869982939 120 38 5.484114249516761 120 39 6.032077191141553 120 40 7.345613054049842 120 41 -5.925775339774269 120 42 -2.8495460391772376 120 43 -8.55449305726806 120 44 -15.172578594447211 120 45 -3.7375150779108317 120 46 -8.557463657631972 120 47 -6.316541827835456 120 48 -4.322076536404662 120 49 -13.568873174546315 120 50 -2.9756015321212814 120 51 1.313632382321412 120 52 1.5458423563329546 120 53 2.3507469885904655 120 54 2.186277676867544 120 55 2.506403509893328 120 56 5.573518589764301 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 353.0 5 67.0 6 324.0 7 187.0 8 436.0 9 731.0 10 351.0 11 414.0 12 447.0 13 206.0 14 286.0 15 265.0 16 308.0 17 428.0 18 486.0 19 599.0 20 832.0 21 999.0 22 1326.0 23 1671.0 24 1987.0 25 2257.0 26 2466.0 27 2593.0 28 2762.0 29 2950.0 30 3328.0 31 3623.0 32 4005.0 33 4371.0 34 4789.0 35 5338.0 36 5706.0 37 6320.0 38 5530.0 39 3611.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 99.19837463546777 0.40219479496358135 0.11609746658742554 0.2833331029812171 8 98.85837491189031 0.4422760631901925 0.5680483186599035 0.13130070625958842 9 98.13688633348076 0.869360902255639 0.2833370190181336 0.7104157452454667 10 64.95376833026964 15.377385872044005 7.916741531104446 11.752104266581897 11 45.2268737992896 22.706729506723978 14.980719527870304 17.085677166116124 12 34.33720785592857 14.57990684560419 21.10624300305447 29.97664229541277 13 26.063881248877035 13.855679792130251 24.18697220571365 35.893466753279064 14 23.205672190510416 20.90998300001382 27.770790430251687 28.11355437922408 15 26.340772818121255 19.052576060404174 24.85565178769709 29.750999333777482 16 31.403518477539276 20.680736888692188 23.684720070812126 24.231024562956406 17 29.93088194636439 18.63699198230578 27.83660492120542 23.595521150124412 18 27.432172819371694 19.180960015479663 27.20412422428925 26.1827429408594 19 30.434301674432753 21.45099069452318 21.713701035631818 26.401006595412248 20 29.84529377577284 23.89920571222982 23.78988736058451 22.465613151412835 21 28.300139593382447 22.271364007021134 24.13030558511741 25.29819081447901 22 24.290630658023854 19.97705692922201 28.76314734703468 26.96916506571946 23 24.55599629593797 21.436567937749647 24.98583334485094 29.021602421461445 24 24.672775773658966 22.73085375461293 25.455073184890324 27.14129728683778 25 27.630960608154805 23.33517622667588 23.357290946786456 25.67657221838286 26 29.491150442477874 21.677267699115042 22.88855088495575 25.943030973451325 27 25.825414609181568 22.05209068149439 21.306554905459425 30.815939803864612 28 24.9340022943705 23.20907796713245 23.659659161587264 28.197260576909787 29 31.547693202109546 22.274822474149744 19.88427944575945 26.293204877981257 30 30.52924174745198 20.959466747797705 24.730677213701927 23.780614291048387 31 30.349022047135254 22.533692722371967 22.135600248807798 24.98168498168498 32 32.4997926515717 23.789776340162007 20.962926101020155 22.747504907246135 33 32.12684708616968 22.997952293984174 21.13979744313465 23.735403176711493 34 26.407022304678307 22.654962825536156 27.93138300081687 23.006631868968668 35 26.018296818125197 24.025299987543768 29.34411027915796 20.61229291517307 36 28.062825777372215 25.0169369668312 23.182214110912938 23.738023144883652 37 28.094381400498204 23.05424854691392 24.912814835316908 23.938555217270967 38 30.32254490561857 25.221931391693325 21.99509742822718 22.460426274460925 39 29.697682462815635 24.10515392597717 20.333448633690764 25.863714977516427 40 23.404966039416546 18.895445941431912 22.049326355639685 35.650261663511856 41 20.780670182193578 16.705728076783572 19.762283418431405 42.751318322591445 42 24.087702521031694 24.905409338489044 23.09536679001566 27.911521350463598 43 21.273412390557464 19.248032805053754 26.584838745428353 32.893716058960436 44 23.146825011445774 21.00195618696153 23.766977899249433 32.08424090234326 45 25.97681408329755 22.237150099031844 25.777365337470048 26.008670480200557 46 22.90809327846365 25.158304582172896 25.134749414584807 26.798852724778648 47 20.190558202197252 22.110029305962584 25.542021416964122 32.15739107487604 48 24.026719404760257 23.127774627629414 26.961428354101262 25.884077613509067 49 22.8275326978497 22.309354910219465 24.300321436488584 30.562790955442253 50 25.164068341040625 23.245091795198405 26.487691413064546 25.103148450696427 51 24.60311958405546 23.832235701906413 27.391334488734838 24.173310225303293 52 23.84486506694729 23.453012281746307 26.885531909001536 25.816590742304868 53 24.19938195474135 21.847761318127016 27.364438839848678 26.588417887282954 54 26.322568136494574 21.86599822734323 27.10087524927986 24.71055838688234 55 23.014761937764224 23.62048652020237 26.567329683276736 26.79742185875667 56 23.915874643243093 22.452824960514285 27.627531934938627 26.003768461304 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.5 9 3.0 10 2.5 11 2.0 12 2.5 13 13.5 14 24.0 15 30.5 16 37.0 17 45.5 18 54.0 19 48.5 20 43.0 21 64.0 22 87.0 23 89.0 24 87.0 25 85.0 26 94.0 27 103.0 28 118.0 29 136.0 30 139.0 31 152.0 32 165.0 33 198.5 34 232.0 35 283.5 36 335.0 37 417.0 38 616.0 39 733.0 40 775.5 41 818.0 42 874.0 43 930.0 44 1025.5 45 1121.0 46 1274.5 47 1924.0 48 2420.0 49 3258.5 50 4097.0 51 4121.5 52 4146.0 53 3864.0 54 3625.0 55 3668.0 56 3776.0 57 3884.0 58 4077.0 59 4270.0 60 4440.5 61 4611.0 62 4954.0 63 5739.0 64 6181.0 65 5976.0 66 5771.0 67 5092.0 68 4413.0 69 4140.0 70 3867.0 71 3342.0 72 2482.5 73 2148.0 74 1841.5 75 1535.0 76 1432.5 77 1330.0 78 953.5 79 449.5 80 322.0 81 236.0 82 150.0 83 149.0 84 148.0 85 98.5 86 49.0 87 28.0 88 4.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0013821126974693516 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.42154437272815226 16 0.06772352217599824 17 0.017967465067101573 18 0.0 19 0.04146338092408055 20 0.12024380467983359 21 0.0 22 0.0 23 0.0 24 0.002764225394938703 25 0.004146338092408055 26 0.0456097190164886 27 0.0773983110582837 28 0.002764225394938703 29 0.15203239672162866 30 0.05804873329371277 31 0.011056901579754813 32 0.015203239672162869 33 0.10642267770514008 34 0.1741461998811383 35 0.13821126974693515 36 0.035934930134203145 37 0.1285364808646497 38 0.20178845383052535 39 0.10780479040260944 40 0.6979669122220226 41 0.9273976200019349 42 0.2764225394938703 43 0.23357704587232045 44 0.3786988791066024 45 0.21284535541028016 46 0.251544510939422 47 0.4892678949041505 48 0.06357718408359019 49 0.24463394745207526 50 0.17552831257860765 51 0.3151216950230122 52 0.1824388760659544 53 0.26260141251917685 54 0.20040634113305597 55 0.28747944107362516 56 0.24186972205713655 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 72353.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.95416914295191 #Duplication Level Percentage of deduplicated Percentage of total 1 88.83041048001384 63.91718380716763 2 6.62107911872611 9.52828493635371 3 1.8747238816004301 4.046825978190262 4 0.8144292272526459 2.3440631349080205 5 0.44371026296075755 1.5963401655771012 6 0.2881235473771153 1.2439014277224165 7 0.15750753923282304 0.7933326883474078 8 0.1651908338295461 0.9508935358589139 9 0.10180365340658074 0.6592677566928807 >10 0.6185052150362075 8.403245200613659 >50 0.0557038858262423 2.7987782123754372 >100 0.02881235473771153 3.717883156192556 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCCTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 482 0.6661783201802275 No Hit GCCCTCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 253 0.349674512459746 No Hit GCCCTCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 238 0.3289428219977057 No Hit GCCCTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 215 0.2971542299559106 No Hit GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 201 0.27780465219133965 No Hit GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 190 0.26260141251917685 No Hit GCCCTCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 171 0.23634127126725912 No Hit GCCCTCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 141 0.19487789034317857 No Hit GCCCTCGGGCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAA 131 0.18105676336848506 No Hit GCCCTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 118 0.1630892983013835 No Hit GCCCTCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 117 0.16170718560391414 No Hit GCCCTCGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC 115 0.15894296020897544 No Hit GCCCTCGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT 109 0.15065028402415934 No Hit GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 105 0.14512183323428193 No Hit GCCCTCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACCACCCATTCGAACGTC 104 0.14373972053681258 No Hit GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 91 0.125772255469711 No Hit GCCCTCGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC 91 0.125772255469711 No Hit GCCCTCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 91 0.125772255469711 No Hit GCCCTCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 89 0.12300803007477229 No Hit GCCCTCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC 85 0.1174795792848949 No Hit GCCCTCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA 84 0.11609746658742554 No Hit GCCCTCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG 79 0.10918690310007878 No Hit GCCCTCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 73 0.10089422691526267 No Hit GCCCTCGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT 73 0.10089422691526267 No Hit GCCCTCGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCG 73 0.10089422691526267 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.012439014277224165 0.0 0.0 0.0 0.0 15 0.012439014277224165 0.0 0.0 0.0 0.0 16 0.01658535236963222 0.0 0.0 0.0 0.0 17 0.01658535236963222 0.0 0.0 0.0 0.0 18 0.01658535236963222 0.0 0.0 0.0 0.0 19 0.019349577764570924 0.0 0.0 0.0 0.0 20 0.022113803159509626 0.0 0.0 0.0 0.0 21 0.023495915856978977 0.0 0.0 0.0 0.0 22 0.029024366646856384 0.0 0.0 0.0 0.0 23 0.031788592041795086 0.0 0.0 0.0 0.0 24 0.031788592041795086 0.0 0.0 0.0 0.0 25 0.034552817436733795 0.0 0.0 0.0 0.0 26 0.03869915552914185 0.0 0.0 0.0 0.0 27 0.0456097190164886 0.0 0.0 0.0 0.0 28 0.04975605710889666 0.0 0.0 0.0 0.0 29 0.05113816980636601 0.0 0.0 0.0 0.0 30 0.05113816980636601 0.0 0.0 0.0 0.0 31 0.05666662059624342 0.0 0.0 0.0 0.0 32 0.05943084599118212 0.0 0.0 0.0 0.0 33 0.05943084599118212 0.0 0.0 0.0 0.0 34 0.06081295868865147 0.0 0.0 0.0 0.0 35 0.06219507138612083 0.0 0.0 0.0 0.0 36 0.06634140947852887 0.0 0.0 0.0 0.0 37 0.06772352217599822 0.0 0.0 0.0 0.0 38 0.06910563487346759 0.0 0.0 0.0 0.0 39 0.07048774757093694 0.0 0.0 0.0 0.0 40 0.07048774757093694 0.0 0.0 0.0 0.0 41 0.07601619836081434 0.0 0.0 0.0 0.0 42 0.07601619836081434 0.0 0.0 0.0 0.0 43 0.0829267618481611 0.0 0.0 0.0 0.0 44 0.0884552126380385 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCACCC 15 0.008179981 50.3352 39 ACCGCTA 15 0.008179981 50.3352 39 ACCGCGA 15 0.008179981 50.3352 39 CACGATT 15 0.008179981 50.3352 47 TCGGACT 15 0.008179981 50.3352 47 CACTATC 15 0.008179981 50.3352 47 AGCGCGA 15 0.008179981 50.3352 47 TCCACGA 15 0.008179981 50.3352 45 ACTCGAA 15 0.008179981 50.3352 46 CCACGAT 15 0.008179981 50.3352 46 ACCTAAT 25 1.9875664E-5 50.335197 40 CGGACAG 15 0.008248138 50.22975 37 CGTCTCG 15 0.008248138 50.22975 42 ATTATAC 15 0.008248138 50.22975 42 TAGACCT 25 2.0124193E-5 50.229748 37 AAACCGC 20 4.0644742E-4 50.229748 37 CTAGACC 30 1.006707E-6 50.1947 36 GGTAAGC 15 0.00827095 50.1947 34 GAGCGTC 15 0.00827095 50.1947 34 CCGAAAA 50 7.2759576E-12 50.1947 36 >>END_MODULE