FastQCFastQC Report
Fri 17 Jun 2016
SRR1525805_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525805_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2269
Sequences flagged as poor quality0
Sequence length40
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC60.2644336712208021No Hit
GCCCTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT60.2644336712208021No Hit
GCCCTCGGGGCCGAATAGCCTCTCCACCCCAGCCGCCCGC40.17628911414720141No Hit
GCCCTCGGGGATTTTAATGACTTTTATTTTAATGAATTTG30.13221683561040104No Hit
GCCCTCGGGATTCTTCCTTCATTTTTTGATAAGTGGTTTA30.13221683561040104No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC30.13221683561040104No Hit
GCCCTCGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT30.13221683561040104No Hit
GCCCTCGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT30.13221683561040104No Hit
GCCCTCGGGGGGAAGTTATATATCATGCTTTGTGCATTGT30.13221683561040104No Hit
GCCCTCTTCACTTACTGCTTCCGTTTTCCCTCTGTTTTGT30.13221683561040104No Hit
GCCCTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT30.13221683561040104No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCCGTGGGTACGCGC30.13221683561040104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTCG2150.033.8444441
CGGGGGG251.4971294E-433.8444446
CCTCGGG2050.033.8444443
GGGGGGG200.00229187133.8444447
CTCGGGG950.033.8444444
CTCGGGC403.727473E-833.8444444
CTCGGGA557.2759576E-1233.8444444
CCCTCGG2100.033.8444442
TCGGGGG452.248271E-933.8444445
TCGGGGC200.00229187133.8444445
TCGGGGA200.00229187133.8444445
TCGGGCT251.4971294E-433.8444445
TCGGGAA356.045557E-733.8444445
Produced by FastQC (version 0.11.5)