##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525805_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2269
Sequences flagged as poor quality	0
Sequence length	40
%GC	58
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.98633759365359	30.0	18.0	33.0	4.0	34.0
2	10.506831203173205	11.0	4.0	12.0	4.0	20.0
3	15.063464081092992	12.0	4.0	24.0	4.0	32.0
4	13.57382106654914	11.0	4.0	20.0	4.0	29.0
5	17.42794182459233	19.0	4.0	28.0	4.0	33.0
6	11.326134861172322	10.0	4.0	14.0	4.0	24.0
7	16.20758043190833	17.0	4.0	26.0	4.0	31.0
8	17.90744821507272	21.0	4.0	29.0	4.0	33.0
9	16.528426619656237	17.0	4.0	27.0	4.0	32.0
10	15.660202732481268	15.0	4.0	25.0	4.0	31.0
11	17.634200088144556	17.0	4.0	30.0	4.0	35.0
12	17.42133098281181	17.0	4.0	30.0	4.0	35.0
13	17.947994711326576	19.0	4.0	30.0	4.0	35.0
14	18.36447774349934	21.0	4.0	31.0	4.0	35.0
15	17.347730277655355	17.0	4.0	30.0	4.0	35.0
16	17.643014543851915	17.0	4.0	31.0	4.0	35.0
17	17.98457470251212	18.0	4.0	31.0	4.0	35.0
18	17.72807404142794	17.0	4.0	31.0	4.0	35.0
19	15.58527985896871	11.0	4.0	28.0	4.0	34.0
20	16.972675187307185	14.0	4.0	31.0	4.0	35.0
21	14.713530189510797	4.0	4.0	28.0	4.0	34.0
22	14.291758483913618	4.0	4.0	27.0	4.0	33.0
23	14.494050242397533	4.0	4.0	28.0	4.0	33.0
24	14.286029087703835	4.0	4.0	28.0	4.0	33.0
25	13.500220361392683	4.0	4.0	27.0	4.0	33.0
26	11.312913177611282	4.0	4.0	18.0	4.0	32.0
27	11.130894667254298	4.0	4.0	18.0	4.0	32.0
28	10.909211106214192	4.0	4.0	17.0	4.0	31.0
29	10.696342000881446	4.0	4.0	17.0	4.0	31.0
30	9.940061701189952	4.0	4.0	12.0	4.0	31.0
31	9.66460996033495	4.0	4.0	4.0	4.0	31.0
32	9.293962097840458	4.0	4.0	4.0	4.0	30.0
33	8.957690612604672	4.0	4.0	4.0	4.0	29.0
34	8.736007051564567	4.0	4.0	4.0	4.0	29.0
35	8.123402379903041	4.0	4.0	4.0	4.0	27.0
36	8.14543851917144	4.0	4.0	4.0	4.0	29.0
37	7.99338915821948	4.0	4.0	4.0	4.0	28.0
38	7.707800793301014	4.0	4.0	4.0	4.0	26.0
39	7.398413397972675	4.0	4.0	4.0	4.0	21.0
40	7.344645218157779	4.0	4.0	4.0	4.0	23.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-7.21533923303835
1	2	8.441297935103243
1	3	-3.926548672566369
1	4	1.7405604719763996
1	5	1.5851032448377573
1	6	2.011209439528022
1	7	3.1073746312684385
1	8	1.5395280235988196
1	9	-6.979203539823011
1	10	0.8089970501474912
1	11	-1.7815634218289063
1	12	0.6414454277286161
1	13	-7.7048672566371685
1	14	0.25811209439528326
1	15	2.5666666666666664
1	16	0.8026548672566385
1	17	-1.1737463126843686
1	18	-4.997197640117996
1	19	-8.860471976401177
1	20	1.359144542772862
1	21	-2.0377581120943944
1	22	-4.7292035398230095
1	23	4.551769911504424
1	24	-8.33746312684366
1	25	-7.686283185840708
1	26	-6.045427728613568
1	27	-5.84188790560472
1	28	-6.056194690265485
1	29	-5.694395280235989
1	30	-5.351769911504425
1	31	-4.919174041297936
1	32	-3.7939528023598825
1	33	-3.9558997050147493
1	34	-3.8587020648967547
1	35	-3.231268436578171
1	36	-3.277433628318584
1	37	-3.5169616519174047
1	38	-3.6330383480825956
1	39	-3.0138643067846607
1	40	-2.882153392330384
2	1	-15.01533923303835
2	2	-5.058702064896757
2	3	-9.92654867256637
2	4	-6.659439528023601
2	5	-9.114896755162242
2	6	-5.788790560471978
2	7	-7.592625368731561
2	8	-10.16047197640118
2	9	-9.479203539823011
2	10	-7.391002949852509
2	11	-8.681563421828907
2	12	-10.658554572271385
2	13	-12.304867256637168
2	14	-9.041887905604717
2	15	-11.733333333333334
2	16	-12.597345132743362
2	17	-13.373746312684368
2	18	-11.097197640117995
2	19	-9.760471976401178
2	20	-10.440855457227137
2	21	-2.7377581120943937
2	22	-1.9292035398230087
2	23	-2.648230088495575
2	24	-7.13746312684366
2	25	-2.886283185840707
2	26	-3.445427728613568
2	27	-2.6418879056047198
2	28	-0.6561946902654849
2	29	-3.8943952802359894
2	30	-2.351769911504425
2	31	-2.7191740412979355
2	32	-1.5939528023598823
2	33	1.8441002949852514
2	34	-2.4587020648967544
2	35	-1.0312684365781708
2	36	-0.2774336283185841
2	37	1.283038348082596
2	38	-1.033038348082596
2	39	2.78613569321534
2	40	1.117846607669616
3	1	4.78466076696165
3	2	-1.0587020648967567
3	3	-5.926548672566369
3	4	-4.7594395280236
3	5	1.5851032448377573
3	6	-3.988790560471978
3	7	-10.392625368731562
3	8	-10.96047197640118
3	9	-9.479203539823011
3	10	-3.6910029498525088
3	11	-6.781563421828906
3	12	-7.358554572271384
3	13	-7.2048672566371685
3	14	0.25811209439528326
3	15	-4.933333333333334
3	16	-8.697345132743362
3	17	1.8262536873156314
3	18	2.002802359882004
3	19	4.139528023598823
3	20	-11.640855457227138
3	21	-8.537758112094394
3	22	-7.7292035398230095
3	23	-8.448230088495576
3	24	-8.33746312684366
3	25	-7.686283185840708
3	26	-6.045427728613568
3	27	-5.84188790560472
3	28	-6.056194690265485
3	29	-5.694395280235989
3	30	-5.351769911504425
3	31	-4.919174041297936
3	32	-3.7939528023598825
3	33	-3.9558997050147493
3	34	-3.8587020648967547
3	35	-3.231268436578171
3	36	-3.277433628318584
3	37	-3.5169616519174047
3	38	-3.6330383480825956
3	39	-3.0138643067846607
3	40	-2.882153392330384
4	1	-20.21533923303835
4	2	-6.058702064896757
4	3	-10.92654867256637
4	4	-9.2594395280236
4	5	-12.914896755162243
4	6	-6.988790560471978
4	7	-12.392625368731562
4	8	-13.96047197640118
4	9	-12.479203539823011
4	10	-11.191002949852509
4	11	-13.281563421828906
4	12	-13.858554572271384
4	13	-13.704867256637169
4	14	-14.241887905604717
4	15	-12.933333333333334
4	16	-13.197345132743362
4	17	-14.173746312684369
4	18	-12.497197640117996
4	19	-10.360471976401177
4	20	-11.640855457227138
4	21	-8.537758112094394
4	22	-7.7292035398230095
4	23	-8.448230088495576
4	24	-8.33746312684366
4	25	-7.686283185840708
4	26	-6.045427728613568
4	27	-5.84188790560472
4	28	-6.056194690265485
4	29	-5.694395280235989
4	30	-5.351769911504425
4	31	-4.919174041297936
4	32	-3.7939528023598825
4	33	-3.9558997050147493
4	34	-3.8587020648967547
4	35	-3.231268436578171
4	36	-3.277433628318584
4	37	-3.5169616519174047
4	38	-3.6330383480825956
4	39	-3.0138643067846607
4	40	-2.882153392330384
5	1	0.5346607669616503
5	2	-4.308702064896757
5	3	-9.17654867256637
5	4	-7.2594395280236
5	5	-6.664896755162243
5	6	-5.738790560471978
5	7	-11.142625368731562
5	8	-12.21047197640118
5	9	-12.229203539823011
5	10	-7.691002949852509
5	11	-8.781563421828906
5	12	-12.358554572271384
5	13	-11.454867256637169
5	14	-9.741887905604717
5	15	-9.683333333333334
5	16	-8.447345132743362
5	17	-7.673746312684369
5	18	-5.997197640117996
5	19	-4.860471976401177
5	20	-4.390855457227138
5	21	-2.7877581120943944
5	22	0.02079646017699055
5	23	-1.9482300884955759
5	24	-0.33746312684366053
5	25	0.3137168141592923
5	26	-0.04542772861356781
5	27	-4.09188790560472
5	28	-3.8061946902654853
5	29	1.5556047197640108
5	30	0.6482300884955752
5	31	-2.6691740412979357
5	32	-2.2939528023598825
5	33	1.0441002949852507
5	34	1.3912979351032453
5	35	0.518731563421829
5	36	0.9725663716814159
5	37	3.7330383480825953
5	38	3.6169616519174044
5	39	-0.7638643067846607
5	40	3.617846607669616
6	1	-8.96533923303835
6	2	-2.8087020648967567
6	3	-7.426548672566369
6	4	1.9905604719763996
6	5	-4.414896755162243
6	6	-2.988790560471978
6	7	-8.392625368731562
6	8	-8.46047197640118
6	9	-7.229203539823011
6	10	-2.9410029498525088
6	11	-7.781563421828906
6	12	-4.608554572271384
6	13	-2.4548672566371685
6	14	-4.491887905604717
6	15	-5.683333333333334
6	16	-3.6973451327433615
6	17	-2.9237463126843686
6	18	-5.497197640117996
6	19	0.13952802359882277
6	20	0.8591445427728619
6	21	-5.787758112094394
6	22	-5.7292035398230095
6	23	-4.948230088495576
6	24	-6.0874631268436605
6	25	-4.186283185840708
6	26	0.9545722713864322
6	27	0.9081120943952801
6	28	0.6938053097345147
6	29	1.0556047197640108
6	30	1.3982300884955752
6	31	2.5808259587020643
6	32	-3.7939528023598825
6	33	-3.9558997050147493
6	34	-3.8587020648967547
6	35	-3.231268436578171
6	36	-3.277433628318584
6	37	-3.5169616519174047
6	38	-3.6330383480825956
6	39	-3.0138643067846607
6	40	-2.882153392330384
7	1	0.7846607669616503
7	2	3.9412979351032433
7	3	7.323451327433631
7	4	16.4905604719764
7	5	8.085103244837757
7	6	11.261209439528022
7	7	4.3573746312684385
7	8	0.7895280235988196
7	9	6.020796460176989
7	10	12.558997050147491
7	11	10.968436578171094
7	12	9.641445427728616
7	13	5.2951327433628315
7	14	15.508112094395283
7	15	17.066666666666666
7	16	8.302654867256638
7	17	12.326253687315631
7	18	-0.24719764011799583
7	19	9.889528023598823
7	20	1.859144542772862
7	21	0.4622418879056056
7	22	6.2707964601769905
7	23	3.801769911504424
7	24	3.4125368731563395
7	25	0.3137168141592923
7	26	7.204572271386432
7	27	5.90811209439528
7	28	8.193805309734515
7	29	7.055604719764011
7	30	8.398230088495575
7	31	-1.1691740412979357
7	32	1.7060471976401175
7	33	3.2941002949852507
7	34	3.3912979351032453
7	35	3.268731563421829
7	36	4.722566371681416
7	37	3.2330383480825953
7	38	3.1169616519174044
7	39	3.7361356932153393
7	40	3.117846607669616
8	1	3.2846607669616503
8	2	0.9412979351032433
8	3	9.57345132743363
8	4	-3.2594395280236004
8	5	-5.414896755162243
8	6	17.011209439528024
8	7	8.607374631268438
8	8	6.53952802359882
8	9	7.020796460176989
8	10	-2.6910029498525088
8	11	14.718436578171094
8	12	15.141445427728616
8	13	14.295132743362831
8	14	9.258112094395283
8	15	10.066666666666666
8	16	14.802654867256638
8	17	-7.173746312684369
8	18	-0.9971976401179958
8	19	2.6395280235988228
8	20	1.359144542772862
8	21	1.9622418879056056
8	22	-4.7292035398230095
8	23	0.051769911504424115
8	24	5.1625368731563395
8	25	-0.6862831858407077
8	26	9.454572271386432
8	27	9.65811209439528
8	28	7.443805309734515
8	29	-5.694395280235989
8	30	-5.351769911504425
8	31	-4.919174041297936
8	32	-3.7939528023598825
8	33	-3.9558997050147493
8	34	-3.8587020648967547
8	35	-3.231268436578171
8	36	-3.277433628318584
8	37	-3.5169616519174047
8	38	-3.6330383480825956
8	39	-3.0138643067846607
8	40	-2.882153392330384
9	1	6.584660766961651
9	2	5.141297935103243
9	3	12.27345132743363
9	4	3.340560471976401
9	5	7.085103244837757
9	6	5.011209439528022
9	7	12.607374631268438
9	8	9.239528023598819
9	9	11.32079646017699
9	10	11.408997050147493
9	11	5.318436578171095
9	12	11.541445427728615
9	13	7.69513274336283
9	14	8.158112094395282
9	15	8.666666666666668
9	16	0.002654867256637772
9	17	3.8262536873156314
9	18	3.9028023598820027
9	19	6.639528023598823
9	20	6.559144542772861
9	21	16.062241887905607
9	22	14.070796460176991
9	23	12.551769911504424
9	24	15.862536873156339
9	25	13.313716814159292
9	26	0.15457227138643148
9	27	0.5581120943952804
9	28	0.34380530973451506
9	29	-2.694395280235989
9	30	0.8482300884955745
9	31	-4.919174041297936
9	32	-3.7939528023598825
9	33	-3.9558997050147493
9	34	-3.8587020648967547
9	35	-3.231268436578171
9	36	-3.277433628318584
9	37	-3.5169616519174047
9	38	-3.6330383480825956
9	39	-3.0138643067846607
9	40	-2.882153392330384
10	1	-5.8820058997050175
10	2	-1.3920353982300906
10	3	-6.259882005899703
10	4	5.073893805309732
10	5	-2.5815634218289087
10	6	-0.988790560471978
10	7	-4.059292035398228
10	8	-0.6271386430678483
10	9	-8.145870206489677
10	10	0.4756637168141573
10	11	1.7184365781710937
10	12	4.141445427728616
10	13	5.961799410029499
10	14	5.424778761061951
10	15	6.733333333333334
10	16	6.135988200589971
10	17	5.159587020648964
10	18	7.836135693215336
10	19	9.972861356932155
10	20	8.02581120943953
10	21	6.795575221238938
10	22	10.937463126843658
10	23	9.885103244837756
10	24	11.66253687315634
10	25	11.647050147492624
10	26	-1.3787610619469017
10	27	1.8247787610619461
10	28	2.9438053097345147
10	29	2.972271386430677
10	30	4.314896755162241
10	31	4.74749262536873
10	32	-3.7939528023598825
10	33	-3.9558997050147493
10	34	-3.8587020648967547
10	35	-3.231268436578171
10	36	-3.277433628318584
10	37	-3.5169616519174047
10	38	-3.6330383480825956
10	39	-3.0138643067846607
10	40	-2.882153392330384
11	1	9.451327433628315
11	2	9.941297935103243
11	3	18.406784660766967
11	4	4.073893805309732
11	5	16.085103244837757
11	6	12.67787610619469
11	7	17.60737463126844
11	8	17.372861356932155
11	9	15.854129793510324
11	10	10.142330383480823
11	11	9.051769911504426
11	12	12.141445427728616
11	13	10.628466076696164
11	14	12.424778761061951
11	15	8.066666666666666
11	16	9.469321533923306
11	17	14.826253687315631
11	18	16.16946902654867
11	19	8.972861356932155
11	20	17.692477876106196
11	21	14.462241887905606
11	22	16.93746312684366
11	23	17.885103244837758
11	24	10.66253687315634
11	25	1.6470501474926262
11	26	3.6212389380530983
11	27	2.15811209439528
11	28	1.9438053097345147
11	29	-1.3610619469026553
11	30	1.981563421828909
11	31	-1.9191740412979357
11	32	-2.1272861356932156
11	33	0.7107669616519168
11	34	-0.5253687315634217
11	35	3.768731563421829
11	36	6.05589970501475
11	37	5.149705014749261
11	38	5.700294985250738
11	39	6.986135693215339
11	40	-2.882153392330384
12	1	-4.8820058997050175
12	2	-1.0587020648967567
12	3	1.0734513274336308
12	4	-4.2594395280236
12	5	2.0851032448377573
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113	12	1.6414454277286161
113	13	-2.2048672566371685
113	14	6.258112094395283
113	15	5.066666666666666
113	16	-0.19734513274336152
113	17	7.826253687315631
113	18	6.002802359882004
113	19	-2.3604719764011772
113	20	1.859144542772862
113	21	18.962241887905606
113	22	1.7707964601769905
113	23	1.0517699115044241
113	24	2.1625368731563395
113	25	-4.186283185840708
113	26	2.954572271386432
113	27	0.6581120943952801
113	28	6.443805309734515
113	29	4.805604719764011
113	30	6.648230088495575
113	31	10.580825958702064
113	32	2.2060471976401175
113	33	5.544100294985251
113	34	10.641297935103246
113	35	6.268731563421829
113	36	7.722566371681416
113	37	3.4830383480825953
113	38	6.366961651917404
113	39	2.4861356932153393
113	40	9.117846607669616
114	1	-1.2153392330383497
114	2	0.9412979351032433
114	3	-2.259882005899703
114	4	2.4072271386430657
114	5	2.0851032448377573
114	6	-2.322123893805312
114	7	2.6073746312684385
114	8	3.0395280235988196
114	9	-1.8125368731563452
114	10	-2.5243362831858427
114	11	-7.281563421828906
114	12	-4.858554572271384
114	13	-4.0382005899705025
114	14	-1.9085545722713846
114	15	-3.2666666666666675
114	16	2.4693215339233063
114	17	-1.5070796460177007
114	18	3.169469026548672
114	19	5.639528023598823
114	20	7.692477876106194
114	21	-4.2044247787610605
114	22	0.9374631268436566
114	23	-4.114896755162242
114	24	0.32920353982300554
114	25	-4.352949852507375
114	26	1.9545722713864322
114	27	2.824778761061946
114	28	-6.056194690265485
114	29	-5.694395280235989
114	30	-5.351769911504425
114	31	-4.919174041297936
114	32	-3.7939528023598825
114	33	-3.9558997050147493
114	34	-3.8587020648967547
114	35	-3.231268436578171
114	36	-3.277433628318584
114	37	-3.5169616519174047
114	38	-3.6330383480825956
114	39	-3.0138643067846607
114	40	-2.882153392330384
115	1	8.451327433628315
115	2	7.941297935103243
115	3	4.073451327433631
115	4	5.7405604719764
115	5	16.75176991150442
115	6	1.344542772861356
115	7	8.94070796460177
115	8	14.03952802359882
115	9	4.187463126843657
115	10	14.808997050147491
115	11	0.7184365781710937
115	12	13.141445427728616
115	13	4.628466076696164
115	14	6.091445427728615
115	15	-1.5999999999999996
115	16	-1.8640117994100276
115	17	-2.8404129793510347
115	18	-1.163864306784662
115	19	-4.027138643067843
115	20	-0.3075221238938042
115	21	-3.5377581120943944
115	22	1.9374631268436566
115	23	1.2184365781710902
115	24	1.3292035398230055
115	25	-7.686283185840708
115	26	-6.045427728613568
115	27	-5.84188790560472
115	28	-6.056194690265485
115	29	-5.694395280235989
115	30	-5.351769911504425
115	31	-4.919174041297936
115	32	-3.7939528023598825
115	33	-3.9558997050147493
115	34	-3.8587020648967547
115	35	-3.231268436578171
115	36	-3.277433628318584
115	37	-3.5169616519174047
115	38	-3.6330383480825956
115	39	-3.0138643067846607
115	40	-2.882153392330384
116	1	5.78466076696165
116	2	3.4412979351032433
116	3	7.073451327433631
116	4	-6.7594395280236
116	5	-6.914896755162243
116	6	-4.488790560471978
116	7	-4.3926253687315615
116	8	-3.9604719764011804
116	9	-9.979203539823011
116	10	-0.6910029498525088
116	11	-7.781563421828906
116	12	-0.8585545722713839
116	13	-8.204867256637169
116	14	-1.2418879056047167
116	15	0.06666666666666643
116	16	1.3026548672566385
116	17	-4.173746312684369
116	18	2.002802359882004
116	19	-10.360471976401177
116	20	-11.640855457227138
116	21	-8.537758112094394
116	22	-7.7292035398230095
116	23	-8.448230088495576
116	24	-8.33746312684366
116	25	-7.686283185840708
116	26	-6.045427728613568
116	27	-5.84188790560472
116	28	-6.056194690265485
116	29	-5.694395280235989
116	30	-5.351769911504425
116	31	-4.919174041297936
116	32	-3.7939528023598825
116	33	-3.9558997050147493
116	34	-3.8587020648967547
116	35	-3.231268436578171
116	36	-3.277433628318584
116	37	-3.5169616519174047
116	38	-3.6330383480825956
116	39	-3.0138643067846607
116	40	-2.882153392330384
117	1	8.78466076696165
117	2	6.941297935103243
117	3	13.07345132743363
117	4	-3.2594395280236004
117	5	13.085103244837757
117	6	-6.988790560471978
117	7	-12.392625368731562
117	8	-13.96047197640118
117	9	-12.479203539823011
117	10	-11.191002949852509
117	11	-13.281563421828906
117	12	-13.858554572271384
117	13	-13.704867256637169
117	14	-14.241887905604717
117	15	-12.933333333333334
117	16	-13.197345132743362
117	17	-14.173746312684369
117	18	-12.497197640117996
117	19	-10.360471976401177
117	20	-11.640855457227138
117	21	-8.537758112094394
117	22	-7.7292035398230095
117	23	-8.448230088495576
117	24	-8.33746312684366
117	25	-7.686283185840708
117	26	-6.045427728613568
117	27	-5.84188790560472
117	28	-6.056194690265485
117	29	-5.694395280235989
117	30	-5.351769911504425
117	31	-4.919174041297936
117	32	-3.7939528023598825
117	33	-3.9558997050147493
117	34	-3.8587020648967547
117	35	-3.231268436578171
117	36	-3.277433628318584
117	37	-3.5169616519174047
117	38	-3.6330383480825956
117	39	-3.0138643067846607
117	40	-2.882153392330384
118	1	-20.21533923303835
118	2	-6.058702064896757
118	3	-10.92654867256637
118	4	-9.2594395280236
118	5	-12.914896755162243
118	6	-6.988790560471978
118	7	-12.392625368731562
118	8	-13.96047197640118
118	9	-12.479203539823011
118	10	-11.191002949852509
118	11	-13.281563421828906
118	12	-13.858554572271384
118	13	-13.704867256637169
118	14	-14.241887905604717
118	15	-12.933333333333334
118	16	-13.197345132743362
118	17	-14.173746312684369
118	18	-12.497197640117996
118	19	-10.360471976401177
118	20	-11.640855457227138
118	21	-8.537758112094394
118	22	-7.7292035398230095
118	23	-8.448230088495576
118	24	-8.33746312684366
118	25	-7.686283185840708
118	26	-6.045427728613568
118	27	-5.84188790560472
118	28	-6.056194690265485
118	29	-5.694395280235989
118	30	-5.351769911504425
118	31	-4.919174041297936
118	32	-3.7939528023598825
118	33	-3.9558997050147493
118	34	-3.8587020648967547
118	35	-3.231268436578171
118	36	-3.277433628318584
118	37	-3.5169616519174047
118	38	-3.6330383480825956
118	39	-3.0138643067846607
118	40	-2.882153392330384
119	1	6.78466076696165
119	2	-6.058702064896757
119	3	-10.92654867256637
119	4	-9.2594395280236
119	5	-12.914896755162243
119	6	-6.988790560471978
119	7	-12.392625368731562
119	8	-13.96047197640118
119	9	-12.479203539823011
119	10	-11.191002949852509
119	11	-13.281563421828906
119	12	-13.858554572271384
119	13	-13.704867256637169
119	14	-14.241887905604717
119	15	-12.933333333333334
119	16	-13.197345132743362
119	17	-14.173746312684369
119	18	-12.497197640117996
119	19	-10.360471976401177
119	20	-11.640855457227138
119	21	-8.537758112094394
119	22	-7.7292035398230095
119	23	-8.448230088495576
119	24	-8.33746312684366
119	25	-7.686283185840708
119	26	-6.045427728613568
119	27	-5.84188790560472
119	28	-6.056194690265485
119	29	-5.694395280235989
119	30	-5.351769911504425
119	31	-4.919174041297936
119	32	-3.7939528023598825
119	33	-3.9558997050147493
119	34	-3.8587020648967547
119	35	-3.231268436578171
119	36	-3.277433628318584
119	37	-3.5169616519174047
119	38	-3.6330383480825956
119	39	-3.0138643067846607
119	40	-2.882153392330384
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	813.0
5	11.0
6	19.0
7	10.0
8	12.0
9	24.0
10	30.0
11	43.0
12	61.0
13	80.0
14	80.0
15	103.0
16	111.0
17	90.0
18	86.0
19	87.0
20	86.0
21	86.0
22	84.0
23	89.0
24	68.0
25	65.0
26	49.0
27	31.0
28	19.0
29	15.0
30	5.0
31	8.0
32	3.0
33	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	97.97178130511463	0.8818342151675485	0.7054673721340388	0.4409171075837742
8	96.29629629629629	0.6172839506172839	1.4109347442680775	1.6754850088183422
9	95.2801058667843	2.6025584472871635	1.102779003087781	1.0145566828407588
10	46.275892463640375	30.85059497576025	11.502864698104892	11.370647862494492
11	34.59673865138828	27.148523578669014	18.64257382106655	19.61216394887616
12	40.72278536800353	13.309828118113707	19.83252534156016	26.13486117232261
13	26.311150286469807	17.893345085940943	26.70780079330101	29.08770383428823
14	21.33098281181137	24.06346408109299	30.498016747465844	24.107536359629794
15	27.545174085500218	19.87659762009696	31.864257382106654	20.713970912296165
16	30.10136624063464	20.18510356985456	27.721463199647424	21.992066989863375
17	25.650066108417807	21.683561040105772	26.928162185985016	25.73821066549141
18	28.162185985015427	20.31732040546496	23.62274129572499	27.897752313794623
19	19.310649580203272	16.924436588599203	18.82456915598763	44.9403446752099
20	25.209343323049804	25.958572058175406	24.195680916703395	24.636403702071398
21	22.34464521815778	23.402379903040988	26.884089907448217	27.36888497135302
22	20.537681798148967	17.49669457910974	27.50110180696342	34.46452181577788
23	25.165271044513	20.18510356985456	31.20317320405465	23.446452181577786
24	24.416042309387397	20.846187747906566	26.84001762891142	27.897752313794623
25	24.47089947089947	20.8994708994709	24.42680776014109	30.202821869488538
26	20.656028368794328	20.96631205673759	29.122340425531917	29.25531914893617
27	18.157778757161747	18.025561921551343	31.776112825033053	32.04054649625386
28	16.019417475728158	18.402471315092676	31.332744924977934	34.24536628420123
29	19.920494699646643	16.29858657243816	32.553003533568905	31.227915194346288
30	17.77679752977503	16.144684605205118	34.98014997794442	31.09836788707543
31	17.33568592853992	18.570798411998236	38.59726510807234	25.496250551389497
32	22.39858906525573	14.37389770723104	37.698412698412696	25.52910052910053
33	18.95107977082415	15.072719259585721	42.970471573380344	23.005729396209784
34	16.747465843984134	14.543851917144115	47.59806081974438	21.11062141912737
35	14.329805996472663	19.1358024691358	43.91534391534391	22.61904761904762
36	13.007054673721342	19.444444444444446	40.07936507936508	27.469135802469136
37	12.224183583406885	16.813768755516328	41.65931156222418	29.302736098852606
38	12.825033054208903	15.998237108858529	41.516086381665936	29.660643455266637
39	16.967827236668136	15.557514323490523	41.38386954605553	26.09078889378581
40	13.442044953724109	16.659321286910533	40.89907448215072	28.999559277214633
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	1.0
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	0.0
24	3.5
25	7.0
26	11.0
27	15.0
28	15.0
29	15.0
30	15.0
31	27.5
32	40.0
33	40.0
34	39.0
35	38.0
36	47.0
37	56.0
38	56.0
39	62.0
40	68.0
41	82.0
42	96.0
43	96.0
44	106.0
45	116.0
46	121.0
47	126.0
48	126.0
49	124.5
50	123.0
51	146.0
52	169.0
53	169.0
54	165.5
55	162.0
56	141.5
57	121.0
58	121.0
59	142.0
60	163.0
61	138.0
62	113.0
63	113.0
64	126.0
65	139.0
66	146.5
67	154.0
68	154.0
69	153.0
70	152.0
71	149.0
72	146.0
73	146.0
74	120.5
75	95.0
76	76.0
77	57.0
78	57.0
79	53.5
80	50.0
81	39.0
82	28.0
83	28.0
84	21.0
85	14.0
86	8.0
87	2.0
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.044072278536800354
8	0.044072278536800354
9	0.08814455707360071
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.2644336712208021
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.044072278536800354
26	0.5729396209784046
27	0.0
28	0.13221683561040104
29	0.22036139268400176
30	0.08814455707360071
31	0.08814455707360071
32	0.044072278536800354
33	0.0
34	0.0
35	0.044072278536800354
36	0.044072278536800354
37	0.13221683561040104
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	2269.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.25385632437197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.97802197802197	93.34508594094315
2	2.4725274725274726	4.759806081974438
3	0.4120879120879121	1.1899515204936095
4	0.045787545787545784	0.17628911414720141
5	0.0	0.0
6	0.09157509157509157	0.5288673424416042
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC	6	0.2644336712208021	No Hit
GCCCTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	6	0.2644336712208021	No Hit
GCCCTCGGGGCCGAATAGCCTCTCCACCCCAGCCGCCCGC	4	0.17628911414720141	No Hit
GCCCTCGGGGATTTTAATGACTTTTATTTTAATGAATTTG	3	0.13221683561040104	No Hit
GCCCTCGGGATTCTTCCTTCATTTTTTGATAAGTGGTTTA	3	0.13221683561040104	No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC	3	0.13221683561040104	No Hit
GCCCTCGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT	3	0.13221683561040104	No Hit
GCCCTCGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.13221683561040104	No Hit
GCCCTCGGGGGGAAGTTATATATCATGCTTTGTGCATTGT	3	0.13221683561040104	No Hit
GCCCTCTTCACTTACTGCTTCCGTTTTCCCTCTGTTTTGT	3	0.13221683561040104	No Hit
GCCCTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.13221683561040104	No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCCGTGGGTACGCGC	3	0.13221683561040104	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.044072278536800354	0.0	0.0	0.0	0.0
19	0.044072278536800354	0.0	0.0	0.0	0.0
20	0.044072278536800354	0.0	0.0	0.0	0.0
21	0.044072278536800354	0.0	0.0	0.0	0.0
22	0.044072278536800354	0.0	0.0	0.0	0.0
23	0.044072278536800354	0.0	0.0	0.0	0.0
24	0.044072278536800354	0.0	0.0	0.0	0.0
25	0.044072278536800354	0.0	0.0	0.0	0.0
26	0.044072278536800354	0.0	0.0	0.0	0.0
27	0.044072278536800354	0.0	0.0	0.0	0.0
28	0.044072278536800354	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCTCG	215	0.0	33.844444	1
CGGGGGG	25	1.4971294E-4	33.844444	6
CCTCGGG	205	0.0	33.844444	3
GGGGGGG	20	0.002291871	33.844444	7
CTCGGGG	95	0.0	33.844444	4
CTCGGGC	40	3.727473E-8	33.844444	4
CTCGGGA	55	7.2759576E-12	33.844444	4
CCCTCGG	210	0.0	33.844444	2
TCGGGGG	45	2.248271E-9	33.844444	5
TCGGGGC	20	0.002291871	33.844444	5
TCGGGGA	20	0.002291871	33.844444	5
TCGGGCT	25	1.4971294E-4	33.844444	5
TCGGGAA	35	6.045557E-7	33.844444	5
>>END_MODULE