FastQCFastQC Report
Fri 17 Jun 2016
SRR1525803_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525803_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4640
Sequences flagged as poor quality0
Sequence length40
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC801.7241379310344827No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG440.9482758620689655No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA190.40948275862068967No Hit
GCCCTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT150.3232758620689655No Hit
GCCCTCGGGACTAATCCTAGCCCTAGCCCTACACAAATAT120.25862068965517243No Hit
GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG120.25862068965517243No Hit
GCCCTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT110.23706896551724138No Hit
GCCCTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA100.21551724137931033No Hit
GCCCTCGGGGGTTGTGTGGCTTTGGCCCTAAGCCATTGTC100.21551724137931033No Hit
GCCCTCGGGGGGGAGGTAGTGACGAAAAATAACAATACAG90.1939655172413793No Hit
GCCCTCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA90.1939655172413793No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAG90.1939655172413793No Hit
GCCCTCGGGCGTCCCTCTTAATCATGGCCTCAGTTCCGAA90.1939655172413793No Hit
GCCCTCGGGGGGGCTGGTGAGCTGGCTCAGTGGGTAAGAG60.12931034482758622No Hit
GCCCTCGGGGGGCTGGCCAGATGGCTCAGCCAGTGAGAAC60.12931034482758622No Hit
GCCCTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.12931034482758622No Hit
GCCCTCGGGATGCCACAACTAGATACATCAACATGATTTA60.12931034482758622No Hit
GCCCTCGGGGAGTTGAAGCTGTTTGACAGCTGGCAGGGTT60.12931034482758622No Hit
GCCCTCGGGGATCCTGCCAGTAGCATATGCTTGTCTCAAA60.12931034482758622No Hit
GCCCTCGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGC60.12931034482758622No Hit
GCCCTCGGGCGGGGAGGTAGTGACGAAAAATAACAATACA60.12931034482758622No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC50.10775862068965517No Hit
GCCCTCGGGGGGGTTGGTGAGATGGCTCAGTGGGTAGGAG50.10775862068965517No Hit
GCCCTCGGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAG50.10775862068965517No Hit
GCCCTCGGGGTGTTCTTTATTAATATCCTAACACTCCTCG50.10775862068965517No Hit
GCCCTCGGGGCCTCTTGTCCGCGTGTCCTCCATTAAATTA50.10775862068965517No Hit
GCCCTCGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGT50.10775862068965517No Hit
GCCCTCGGGATGGCCTACCCATTCCAACTTGGTCTACAAG50.10775862068965517No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGC50.10775862068965517No Hit
GCCCTCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC50.10775862068965517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG850.033.8695686
CCTCGGG4500.033.8695683
CCCTCGG4550.033.8695682
TCGGGGT358.364532E-733.8695685
TCGGGGG1650.033.8695685
TCGGGAG358.364532E-733.8695685
CGGGGTG251.7719211E-433.8695646
CGGGGGT301.2208155E-533.8695646
CGGGGGA200.002559227433.8695646
CGGGTCA200.002559227433.8695646
CTCGGGT301.2208155E-533.8695644
CTCGGGG3000.033.8695644
CTCGGGC453.8489816E-933.8695644
CTCGGGA750.033.8695644
TCTTCTG200.002559227433.86956420
TCGGGTC200.002559227433.8695645
TCGGGGC251.7719211E-433.8695645
TCGGGGA750.033.8695645
TCGGGCG200.002559227433.8695645
TCGGGAA251.7719211E-433.8695645
Produced by FastQC (version 0.11.5)