##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525803_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4640
Sequences flagged as poor quality	0
Sequence length	40
%GC	56
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.09762931034483	33.0	30.0	35.0	4.0	38.0
2	21.489224137931036	24.0	4.0	34.0	4.0	37.0
3	21.554956896551722	27.0	4.0	33.0	4.0	36.0
4	20.93362068965517	25.0	4.0	34.0	4.0	36.0
5	19.995258620689654	23.0	4.0	33.0	4.0	36.0
6	16.51185344827586	12.0	4.0	30.0	4.0	35.0
7	15.548060344827586	10.0	4.0	29.0	4.0	34.0
8	15.539870689655173	4.0	4.0	30.0	4.0	34.0
9	15.018534482758621	4.0	4.0	29.0	4.0	34.0
10	14.47801724137931	4.0	4.0	29.0	4.0	34.0
11	11.945043103448276	4.0	4.0	23.0	4.0	33.0
12	11.360560344827586	4.0	4.0	21.0	4.0	31.0
13	10.482974137931034	4.0	4.0	17.0	4.0	30.0
14	10.41594827586207	4.0	4.0	15.0	4.0	31.0
15	10.260344827586207	4.0	4.0	11.0	4.0	32.0
16	8.104525862068966	4.0	4.0	4.0	4.0	25.0
17	7.591810344827586	4.0	4.0	4.0	4.0	22.0
18	7.6075431034482754	4.0	4.0	4.0	4.0	23.0
19	7.180818965517242	4.0	4.0	4.0	4.0	20.0
20	7.1377155172413795	4.0	4.0	4.0	4.0	20.0
21	6.804956896551724	4.0	4.0	4.0	4.0	18.0
22	6.864008620689655	4.0	4.0	4.0	4.0	19.0
23	7.088577586206896	4.0	4.0	4.0	4.0	21.0
24	6.7691810344827585	4.0	4.0	4.0	4.0	18.0
25	6.960991379310345	4.0	4.0	4.0	4.0	20.0
26	7.078879310344828	4.0	4.0	4.0	4.0	20.0
27	7.052586206896552	4.0	4.0	4.0	4.0	20.0
28	7.147844827586207	4.0	4.0	4.0	4.0	22.0
29	6.77176724137931	4.0	4.0	4.0	4.0	18.0
30	7.010560344827586	4.0	4.0	4.0	4.0	20.0
31	7.467025862068965	4.0	4.0	4.0	4.0	25.0
32	7.3357758620689655	4.0	4.0	4.0	4.0	24.0
33	7.291379310344827	4.0	4.0	4.0	4.0	23.0
34	7.341810344827586	4.0	4.0	4.0	4.0	25.0
35	7.150431034482758	4.0	4.0	4.0	4.0	22.0
36	7.23448275862069	4.0	4.0	4.0	4.0	25.0
37	7.115948275862069	4.0	4.0	4.0	4.0	23.0
38	6.969827586206897	4.0	4.0	4.0	4.0	20.0
39	6.785129310344828	4.0	4.0	4.0	4.0	17.0
40	6.8293103448275865	4.0	4.0	4.0	4.0	20.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2	1	6.687334125569414
2	2	1.5982995218289346
2	3	4.846964745494159
2	4	1.7893458393458417
2	5	7.64901854681267
2	6	-1.1039346546699473
2	7	0.2966368870780638
2	8	0.9412740853917274
2	9	-5.777605890841182
2	10	-1.1116250035367692
2	11	-0.1285505616387983
2	12	6.869960458931047
2	13	4.9687484084542906
2	14	8.417756698639055
2	15	9.132910335851513
2	16	5.836628186628188
2	17	2.209068627450981
2	18	8.421243598449482
2	19	8.548389355742298
2	20	7.112729182582124
2	21	3.387406629318395
2	22	1.367276759188524
2	23	5.653917678182386
2	24	5.87750756868404
2	25	4.91296437767026
2	26	3.562112723877428
2	27	4.688013185072009
2	28	4.858230413377472
2	29	4.402222505163682
2	30	3.4583407605466414
2	31	4.184781286251875
2	32	3.4157251789604723
2	33	5.490648784766431
2	34	6.701293042469514
2	35	5.5778350734233095
2	36	8.525204425204425
2	37	6.852875392581273
2	38	8.332231276348924
2	39	8.91352071131483
2	40	8.429028732705202
3	1	1.6373341255694136
3	2	-1.9017004781710654
3	3	-2.7780352545058413
3	4	-0.5356541606541576
3	5	3.9490185468126704
3	6	-0.8789346546699477
3	7	4.371636887078063
3	8	5.266274085391727
3	9	-3.0026058908411812
3	10	1.8133749964632315
3	11	-6.503550561638798
3	12	-2.230039541068953
3	13	-4.331251591545709
3	14	-1.5072433013609459
3	15	-4.5670896641484875
3	16	-0.03837181337181228
3	17	-1.1409313725490184
3	18	1.821243598449482
3	19	-1.0516106442577025
3	20	0.7377291825821244
3	21	-1.0375933706816047
3	22	-1.5327232408114764
3	23	-1.5960823218176143
3	24	1.8275075686840392
3	25	-1.5370356223297401
3	26	2.687112723877428
3	27	2.9630131850720094
3	28	0.5332304133774715
3	29	2.052222505163682
3	30	2.1083407605466418
3	31	4.109781286251875
3	32	3.215725178960473
3	33	1.56564878476643
3	34	4.126293042469515
3	35	4.12783507342331
3	36	3.325204425204425
3	37	3.277875392581274
3	38	4.182231276348923
3	39	4.388520711314829
3	40	4.079028732705203
5	1	3.2706674589027465
5	2	5.098299521828935
5	3	10.221964745494159
5	4	10.664345839345842
5	5	11.315685213479338
5	6	8.021065345330053
5	7	-2.66169644625527
5	8	-2.6003925812749387
5	9	0.5973941091588184
5	10	5.680041663129899
5	11	0.16311610502786777
5	12	-2.463372874402287
5	13	-2.1979182582123764
5	14	-1.2072433013609452
5	15	-5.367089664148487
5	16	-3.0383718133718123
5	17	-2.540931372549019
5	18	-2.5787564015505184
5	19	-2.451610644257703
5	20	-2.2622708174178756
5	21	-2.237593370681605
5	22	-2.132723240811476
5	23	-2.5960823218176143
5	24	-2.37249243131596
5	25	-2.33703562232974
5	26	-2.312887276122572
5	27	-2.436986814927991
5	28	-2.2667695866225284
5	29	-2.3477774948363184
5	30	-2.2916592394533586
5	31	-2.6902187137481253
5	32	-2.5842748210395277
5	33	-2.6343512152335693
5	34	-2.673706957530486
5	35	-2.6721649265766905
5	36	-2.474795574795576
5	37	-2.5221246074187267
5	38	-2.4177687236510765
5	39	-2.2114792886851706
5	40	-2.320971267294797
6	1	3.9373341255694143
6	2	3.8482995218289346
6	3	-9.778035254505841
6	4	1.9143458393458417
6	5	12.27401854681267
6	6	15.021065345330053
6	7	7.796636887078064
6	8	14.441274085391727
6	9	14.722394109158818
6	10	4.513374996463231
6	11	9.746449438361202
6	12	11.744960458931047
6	13	12.96874840845429
6	14	12.292756698639055
6	15	20.132910335851513
6	16	16.08662818662819
6	17	13.209068627450982
6	18	21.171243598449482
6	19	17.423389355742298
6	20	15.987729182582125
6	21	14.512406629318395
6	22	8.367276759188524
6	23	18.653917678182385
6	24	14.37750756868404
6	25	8.41296437767026
6	26	12.187112723877428
6	27	18.31301318507201
6	28	16.48323041337747
6	29	12.527222505163682
6	30	13.83334076054664
6	31	20.309781286251877
6	32	13.665725178960471
6	33	14.615648784766432
6	34	10.451293042469514
6	35	16.20283507342331
6	36	19.400204425204425
6	37	19.352875392581275
6	38	17.207231276348924
6	39	17.78852071131483
6	40	18.429028732705202
7	1	7.77066745890275
7	2	15.598299521828935
7	3	15.555298078827494
7	4	16.831012506012506
7	5	18.315685213479334
7	6	16.02106534533005
7	7	9.338303553744732
7	8	18.73294075205839
7	9	12.43072744249215
7	10	15.180041663129899
7	11	6.496449438361202
7	12	12.203293792264379
7	13	7.635415075120958
7	14	3.1260900319723888
7	15	2.1329103358515127
7	16	3.2949615199615216
7	17	3.6257352941176473
7	18	4.421243598449482
7	19	7.381722689075631
7	20	2.9043958492487905
7	21	8.929073295985061
7	22	11.700610092521856
7	23	8.73725101151572
7	24	10.960840902017372
7	25	7.996297711003594
7	26	9.020446057210762
7	27	8.396346518405343
7	28	10.066563746710804
7	29	11.152222505163682
7	30	11.541674093879973
7	31	9.809781286251875
7	32	10.58239184562714
7	33	6.198982118099765
7	34	7.826293042469514
7	35	7.8278350734233095
7	36	8.525204425204425
7	37	10.311208725914605
7	38	11.248897943015592
7	39	4.788520711314829
7	40	3.012362066038537
8	1	0.1516198398551296
8	2	4.526870950400362
8	3	7.650536174065586
8	4	5.092917267917269
8	5	7.863304261098385
8	6	9.092493916758626
8	7	11.457351172792348
8	8	8.780559799677443
8	9	4.883108394873103
8	10	12.01337499646323
8	11	7.782163724075486
8	12	10.94138903035962
8	13	8.754462694168575
8	14	12.292756698639055
8	15	6.918624621565797
8	16	3.9616281866281877
8	17	1.316211484593838
8	18	5.135529312735195
8	19	5.548389355742297
8	20	1.7377291825821244
8	21	2.0481209150326816
8	22	3.438705330617095
8	23	3.975346249610957
8	24	-0.9439210027445313
8	25	5.520107234813118
8	26	7.258541295305999
8	27	4.8487274707862955
8	28	4.447516127663185
8	29	4.652222505163682
8	30	4.565483617689499
8	31	7.024067000537588
8	32	6.415725178960472
8	33	7.222791641909288
8	34	8.326293042469514
8	35	7.899263644851881
8	36	6.525204425204424
8	37	9.620732535438416
8	38	10.582231276348924
8	39	4.074234997029116
8	40	6.250457304133774
9	1	-11.419808731573443
9	2	-13.330271906742494
9	3	-9.492320968791555
9	4	-7.478511303511301
9	5	-10.708124310330188
9	6	-7.621791797527091
9	7	-7.828363112921936
9	8	-9.933725914608273
9	9	-9.688320176555468
9	10	-7.843767860679626
9	11	-3.6464077044959415
9	12	-2.058610969640382
9	13	-5.5312515915457094
9	14	-5.707243301360945
9	15	-5.367089664148487
9	16	-3.0383718133718123
9	17	-2.540931372549019
9	18	-2.5787564015505184
9	19	-2.451610644257703
9	20	-2.2622708174178756
9	21	-2.237593370681605
9	22	-2.132723240811476
9	23	-2.5960823218176143
9	24	-2.37249243131596
9	25	-2.33703562232974
9	26	-2.312887276122572
9	27	-2.436986814927991
9	28	-2.2667695866225284
9	29	-2.3477774948363184
9	30	-2.2916592394533586
9	31	-2.6902187137481253
9	32	-2.5842748210395277
9	33	-2.6343512152335693
9	34	-2.673706957530486
9	35	-2.6721649265766905
9	36	-2.474795574795576
9	37	-2.5221246074187267
9	38	-2.4177687236510765
9	39	-2.2114792886851706
9	40	-2.320971267294797
10	1	3.9373341255694143
10	2	-3.9017004781710654
10	3	-2.2780352545058413
10	4	-0.8356541606541583
10	5	-3.3509814531873303
10	6	0.021065345330052665
10	7	-2.328363112921936
10	8	-0.9337259146082726
10	9	-5.402605890841182
10	10	3.013374996463231
10	11	6.996449438361202
10	12	-0.1300395410689532
10	13	9.96874840845429
10	14	9.792756698639055
10	15	0.6329103358515127
10	16	5.961628186628188
10	17	9.459068627450982
10	18	0.42124359844948156
10	19	1.5483893557422972
10	20	10.737729182582125
10	21	11.262406629318395
10	22	5.367276759188524
10	23	9.403917678182385
10	24	11.12750756868404
10	25	11.16296437767026
10	26	13.187112723877428
10	27	13.063013185072009
10	28	12.733230413377472
10	29	13.652222505163682
10	30	14.70834076054664
10	31	7.309781286251875
10	32	9.415725178960471
10	33	8.865648784766432
10	34	10.326293042469514
10	35	11.827835073423309
10	36	-2.474795574795576
10	37	-2.5221246074187267
10	38	-2.4177687236510765
10	39	-2.2114792886851706
10	40	-2.320971267294797
11	1	5.0736977619330474
11	2	0.18920861273802458
11	3	-1.596217072687658
11	4	-5.790199615199613
11	5	0.42174581953994306
11	6	-9.115298291033584
11	7	-4.373817658376481
11	8	-8.024635005517364
11	9	-7.584424072659363
11	10	-4.8957159126276775
11	11	-1.8671869252751616
11	12	-5.26640317743259
11	13	-1.6221606824548003
11	14	-1.8890614831791268
11	15	-2.63981693687576
11	16	-0.9474627224627215
11	17	1.4590686274509812
11	18	-1.0333018560959726
11	19	2.548389355742297
11	20	0.8286382734912152
11	21	-1.237593370681605
11	22	1.0490949410067056
11	23	-0.5960823218176143
11	24	1.082053023229494
11	25	2.2993280140338967
11	26	-1.767432730668026
11	27	-1.3460777240189001
11	28	0.18777586792292578
11	29	0.37949523243640915
11	30	-1.2007501485442678
11	31	-0.8720368955663069
11	32	-0.5842748210395277
11	33	0.0020124211300673878
11	34	-0.037343321166849464
11	35	0.600562346150582
11	36	2.979749879749879
11	37	3.3869663016721816
11	38	2.854958549076197
11	39	2.424884347678466
11	40	3.133574187250658
12	1	4.659556347791636
12	2	10.209410632940045
12	3	13.666409189938602
12	4	4.886568061568063
12	5	13.14901854681267
12	6	11.07662090088561
12	7	13.282747998189175
12	8	13.732940752058395
12	9	13.93072744249215
12	10	14.235597218685452
12	11	11.385338327250091
12	12	15.147738236708825
12	13	17.13541507512096
12	14	16.403867809750167
12	15	20.744021446962623
12	16	16.739405964405968
12	17	12.792401960784314
12	18	9.310132487338372
12	19	7.881722689075631
12	20	10.737729182582125
12	21	4.095739962651729
12	22	5.200610092521858
12	23	5.181695455960164
12	24	6.4052853464618185
12	25	5.218519933225815
12	26	7.353779390544094
12	27	6.118568740627564
12	28	7.066563746710806
12	29	4.207778060719237
12	30	8.375007427213308
12	31	8.86533684180743
12	32	11.30461406784936
12	33	11.921204340321989
12	34	12.548515264691735
12	35	12.327835073423309
12	36	10.747426647426646
12	37	9.14454205924794
12	38	11.026675720793367
12	39	7.121854044648163
12	40	11.34569539937187
14	1	-4.895999207763918
14	2	-12.901700478171065
14	3	-13.44470192117251
14	4	-7.002320827320824
14	5	-5.517648119853996
14	6	-1.8122679880032813
14	7	-10.828363112921936
14	8	-10.933725914608273
14	9	-10.402605890841182
14	10	-9.98662500353677
14	11	-7.503550561638798
14	12	-6.630039541068953
14	13	-5.5312515915457094
14	14	-5.707243301360945
14	15	-5.367089664148487
14	16	-3.0383718133718123
14	17	-2.540931372549019
14	18	-2.5787564015505184
14	19	-2.451610644257703
14	20	-2.2622708174178756
14	21	-2.237593370681605
14	22	-2.132723240811476
14	23	-2.5960823218176143
14	24	-2.37249243131596
14	25	-2.33703562232974
14	26	-2.312887276122572
14	27	-2.436986814927991
14	28	-2.2667695866225284
14	29	-2.3477774948363184
14	30	-2.2916592394533586
14	31	-2.6902187137481253
14	32	-2.5842748210395277
14	33	-2.6343512152335693
14	34	-2.673706957530486
14	35	-2.6721649265766905
14	36	-2.474795574795576
14	37	-2.5221246074187267
14	38	-2.4177687236510765
14	39	-2.2114792886851706
14	40	-2.320971267294797
15	1	-0.5626658744305857
15	2	-4.758843335313923
15	3	-8.492320968791555
15	4	-2.192797017797016
15	5	-1.136695738901615
15	6	2.235351059615768
15	7	5.314494029935206
15	8	6.6377026568203
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110	25	-2.33703562232974
110	26	-2.312887276122572
110	27	-2.436986814927991
110	28	-2.2667695866225284
110	29	-2.3477774948363184
110	30	-2.2916592394533586
110	31	-2.6902187137481253
110	32	-2.5842748210395277
110	33	-2.6343512152335693
110	34	-2.673706957530486
110	35	-2.6721649265766905
110	36	-2.474795574795576
110	37	-2.5221246074187267
110	38	-2.4177687236510765
110	39	-2.2114792886851706
110	40	-2.320971267294797
111	1	7.437334125569414
111	2	10.431632855162267
111	3	11.888631412160827
111	4	9.997679172679174
111	5	7.482351880146002
111	6	10.521065345330053
111	7	9.171636887078064
111	8	4.39960741872506
111	9	14.264060775825486
111	10	12.346708329796563
111	11	23.163116105027868
111	12	19.70329379226438
111	13	20.13541507512096
111	14	19.292756698639053
111	15	23.632910335851513
111	16	1.9616281866281877
111	17	-0.5409313725490188
111	18	3.4212435984494816
111	19	-1.1182773109243698
111	20	-0.9289374840845426
111	21	2.762406629318395
111	22	-0.13272324081147602
111	23	2.4039176781823857
111	24	-1.37249243131596
111	25	2.329631044336926
111	26	3.020446057210762
111	27	-1.436986814927991
111	28	-0.9334362532891953
111	29	3.6522225051636816
111	30	4.041674093879975
111	31	4.309781286251875
111	32	-0.5842748210395277
111	33	0.6989821180997637
111	34	3.99295970913618
111	35	4.3278350734233095
111	36	7.858537758537758
111	37	8.14454205924794
111	38	7.915564609682257
111	39	8.121854044648163
111	40	6.345695399371869
112	1	-4.134094445859159
112	2	-14.330271906742494
112	3	-11.920892397362984
112	4	-11.4785113035113
112	5	-13.85098145318733
112	6	-11.478934654669947
112	7	-10.828363112921936
112	8	-10.933725914608273
112	9	-10.402605890841182
112	10	-9.98662500353677
112	11	-7.503550561638798
112	12	-6.630039541068953
112	13	-5.5312515915457094
112	14	-5.707243301360945
112	15	-5.367089664148487
112	16	-3.0383718133718123
112	17	-2.540931372549019
112	18	-2.5787564015505184
112	19	-2.451610644257703
112	20	-2.2622708174178756
112	21	-2.237593370681605
112	22	-2.132723240811476
112	23	-2.5960823218176143
112	24	-2.37249243131596
112	25	-2.33703562232974
112	26	-2.312887276122572
112	27	-2.436986814927991
112	28	-2.2667695866225284
112	29	-2.3477774948363184
112	30	-2.2916592394533586
112	31	-2.6902187137481253
112	32	-2.5842748210395277
112	33	-2.6343512152335693
112	34	-2.673706957530486
112	35	-2.6721649265766905
112	36	-2.474795574795576
112	37	-2.5221246074187267
112	38	-2.4177687236510765
112	39	-2.2114792886851706
112	40	-2.320971267294797
113	1	-10.062665874430586
113	2	-3.4017004781710654
113	3	-15.778035254505841
113	4	-15.335654160654158
113	5	-13.85098145318733
113	6	-11.478934654669947
113	7	-10.828363112921936
113	8	-10.933725914608273
113	9	-10.402605890841182
113	10	-9.98662500353677
113	11	-7.503550561638798
113	12	-6.630039541068953
113	13	-5.5312515915457094
113	14	-5.707243301360945
113	15	-5.367089664148487
113	16	-3.0383718133718123
113	17	-2.540931372549019
113	18	-2.5787564015505184
113	19	-2.451610644257703
113	20	-2.2622708174178756
113	21	-2.237593370681605
113	22	-2.132723240811476
113	23	-2.5960823218176143
113	24	-2.37249243131596
113	25	-2.33703562232974
113	26	-2.312887276122572
113	27	-2.436986814927991
113	28	-2.2667695866225284
113	29	-2.3477774948363184
113	30	-2.2916592394533586
113	31	-2.6902187137481253
113	32	-2.5842748210395277
113	33	-2.6343512152335693
113	34	-2.673706957530486
113	35	-2.6721649265766905
113	36	-2.474795574795576
113	37	-2.5221246074187267
113	38	-2.4177687236510765
113	39	-2.2114792886851706
113	40	-2.320971267294797
114	1	-1.5626658744305857
114	2	4.098299521828935
114	3	5.221964745494159
114	4	2.3310125060125095
114	5	5.482351880146002
114	6	6.1877320119967205
114	7	4.171636887078064
114	8	0.39960741872506134
114	9	-3.0692725575078477
114	10	4.680041663129899
114	11	11.16311610502787
114	12	-2.2967062077356193
114	13	3.4687484084542906
114	14	-5.707243301360945
114	15	-5.367089664148487
114	16	-3.0383718133718123
114	17	-2.540931372549019
114	18	-2.5787564015505184
114	19	-2.451610644257703
114	20	-2.2622708174178756
114	21	-2.237593370681605
114	22	-2.132723240811476
114	23	-2.5960823218176143
114	24	-2.37249243131596
114	25	-2.33703562232974
114	26	-2.312887276122572
114	27	-2.436986814927991
114	28	-2.2667695866225284
114	29	-2.3477774948363184
114	30	-2.2916592394533586
114	31	-2.6902187137481253
114	32	-2.5842748210395277
114	33	-2.6343512152335693
114	34	-2.673706957530486
114	35	-2.6721649265766905
114	36	-2.474795574795576
114	37	-2.5221246074187267
114	38	-2.4177687236510765
114	39	-2.2114792886851706
114	40	-2.320971267294797
115	1	8.837334125569413
115	2	4.498299521828933
115	3	5.421964745494158
115	4	10.264345839345843
115	5	1.5490185468126683
115	6	-8.878934654669948
115	7	-6.228363112921937
115	8	-6.533725914608272
115	9	-7.402605890841182
115	10	0.6133749964632305
115	11	2.4964494383612017
115	12	2.9699604589310464
115	13	-0.33125159154571016
115	14	0.09275669863905556
115	15	-1.167089664148488
115	16	-1.638371813371812
115	17	-1.740931372549019
115	18	-1.3787564015505183
115	19	-1.4516106442577028
115	20	-1.4622708174178758
115	21	4.1624066293183954
115	22	0.4672767591885236
115	23	-0.19608232181761398
115	24	3.2275075686840395
115	25	4.26296437767026
115	26	-2.312887276122572
115	27	-2.436986814927991
115	28	-2.2667695866225284
115	29	-2.3477774948363184
115	30	-2.2916592394533586
115	31	-2.6902187137481253
115	32	-2.5842748210395277
115	33	-2.6343512152335693
115	34	-2.673706957530486
115	35	-2.6721649265766905
115	36	-2.474795574795576
115	37	-2.5221246074187267
115	38	-2.4177687236510765
115	39	-2.2114792886851706
115	40	-2.320971267294797
116	1	6.437334125569414
116	2	10.098299521828935
116	3	4.721964745494159
116	4	-7.585654160654158
116	5	-6.60098145318733
116	6	-11.478934654669947
116	7	-10.828363112921936
116	8	-10.933725914608273
116	9	-10.402605890841182
116	10	-9.98662500353677
116	11	-7.503550561638798
116	12	-6.630039541068953
116	13	-5.5312515915457094
116	14	-5.707243301360945
116	15	-5.367089664148487
116	16	-3.0383718133718123
116	17	-2.540931372549019
116	18	-2.5787564015505184
116	19	-2.451610644257703
116	20	-2.2622708174178756
116	21	-2.237593370681605
116	22	-2.132723240811476
116	23	-2.5960823218176143
116	24	-2.37249243131596
116	25	-2.33703562232974
116	26	-2.312887276122572
116	27	-2.436986814927991
116	28	-2.2667695866225284
116	29	-2.3477774948363184
116	30	-2.2916592394533586
116	31	-2.6902187137481253
116	32	-2.5842748210395277
116	33	-2.6343512152335693
116	34	-2.673706957530486
116	35	-2.6721649265766905
116	36	-2.474795574795576
116	37	-2.5221246074187267
116	38	-2.4177687236510765
116	39	-2.2114792886851706
116	40	-2.320971267294797
117	1	8.437334125569414
117	2	8.598299521828935
117	3	11.721964745494159
117	4	15.164345839345842
117	5	12.14901854681267
117	6	8.521065345330053
117	7	1.1716368870780638
117	8	15.816274085391727
117	9	18.09739410915882
117	10	0.7633749964632308
117	11	5.746449438361202
117	12	5.619960458931047
117	13	1.9687484084542906
117	14	4.042756698639055
117	15	3.1329103358515127
117	16	9.461628186628188
117	17	9.959068627450982
117	18	-0.5787564015505184
117	19	3.548389355742297
117	20	2.9877291825821244
117	21	5.012406629318395
117	22	4.367276759188524
117	23	4.653917678182386
117	24	3.12750756868404
117	25	-0.5870356223297399
117	26	5.937112723877428
117	27	4.813013185072009
117	28	5.983230413377472
117	29	5.902222505163682
117	30	4.958340760546641
117	31	4.059781286251875
117	32	5.165725178960472
117	33	3.6156487847664307
117	34	5.576293042469514
117	35	5.5778350734233095
117	36	-2.474795574795576
117	37	-2.5221246074187267
117	38	-2.4177687236510765
117	39	-2.2114792886851706
117	40	-2.320971267294797
118	1	4.104000792236082
118	2	-0.2350338115043975
118	3	-7.111368587839175
118	4	-15.335654160654158
118	5	-13.85098145318733
118	6	-11.478934654669947
118	7	-10.828363112921936
118	8	-10.933725914608273
118	9	-10.402605890841182
118	10	-9.98662500353677
118	11	-7.503550561638798
118	12	-6.630039541068953
118	13	-5.5312515915457094
118	14	-5.707243301360945
118	15	-5.367089664148487
118	16	-3.0383718133718123
118	17	-2.540931372549019
118	18	-2.5787564015505184
118	19	-2.451610644257703
118	20	-2.2622708174178756
118	21	-2.237593370681605
118	22	-2.132723240811476
118	23	-2.5960823218176143
118	24	-2.37249243131596
118	25	-2.33703562232974
118	26	-2.312887276122572
118	27	-2.436986814927991
118	28	-2.2667695866225284
118	29	-2.3477774948363184
118	30	-2.2916592394533586
118	31	-2.6902187137481253
118	32	-2.5842748210395277
118	33	-2.6343512152335693
118	34	-2.673706957530486
118	35	-2.6721649265766905
118	36	-2.474795574795576
118	37	-2.5221246074187267
118	38	-2.4177687236510765
118	39	-2.2114792886851706
118	40	-2.320971267294797
119	1	-0.5626658744305857
119	2	4.598299521828935
119	3	3.7219647454941587
119	4	7.164345839345842
119	5	2.3990185468126697
119	6	-3.7289346546699473
119	7	-5.078363112921936
119	8	1.8162740853917274
119	9	-3.1526058908411816
119	10	-5.736625003536769
119	11	-6.003550561638798
119	12	0.6199604589310468
119	13	-5.5312515915457094
119	14	-5.707243301360945
119	15	-5.367089664148487
119	16	-3.0383718133718123
119	17	-2.540931372549019
119	18	-2.5787564015505184
119	19	-2.451610644257703
119	20	-2.2622708174178756
119	21	-2.237593370681605
119	22	-2.132723240811476
119	23	-2.5960823218176143
119	24	-2.37249243131596
119	25	-2.33703562232974
119	26	-2.312887276122572
119	27	-2.436986814927991
119	28	-2.2667695866225284
119	29	-2.3477774948363184
119	30	-2.2916592394533586
119	31	-2.6902187137481253
119	32	-2.5842748210395277
119	33	-2.6343512152335693
119	34	-2.673706957530486
119	35	-2.6721649265766905
119	36	-2.474795574795576
119	37	-2.5221246074187267
119	38	-2.4177687236510765
119	39	-2.2114792886851706
119	40	-2.320971267294797
120	1	-7.2293325410972535
120	2	3.431632855162267
120	3	3.2219647454941587
120	4	4.997679172679174
120	5	1.1490185468126697
120	6	-0.8122679880032813
120	7	-2.4950297795886023
120	8	-0.2670592479416065
120	9	0.2640607758254845
120	10	-0.3199583368701031
120	11	-7.503550561638798
120	12	-6.630039541068953
120	13	-5.5312515915457094
120	14	-5.707243301360945
120	15	-5.367089664148487
120	16	-3.0383718133718123
120	17	-2.540931372549019
120	18	-2.5787564015505184
120	19	-2.451610644257703
120	20	-2.2622708174178756
120	21	-2.237593370681605
120	22	-2.132723240811476
120	23	-2.5960823218176143
120	24	-2.37249243131596
120	25	-2.33703562232974
120	26	-2.312887276122572
120	27	-2.436986814927991
120	28	-2.2667695866225284
120	29	-2.3477774948363184
120	30	-2.2916592394533586
120	31	-2.6902187137481253
120	32	-2.5842748210395277
120	33	-2.6343512152335693
120	34	-2.673706957530486
120	35	-2.6721649265766905
120	36	-2.474795574795576
120	37	-2.5221246074187267
120	38	-2.4177687236510765
120	39	-2.2114792886851706
120	40	-2.320971267294797
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1290.0
5	203.0
6	310.0
7	370.0
8	258.0
9	254.0
10	335.0
11	282.0
12	189.0
13	135.0
14	113.0
15	82.0
16	46.0
17	29.0
18	25.0
19	27.0
20	49.0
21	73.0
22	93.0
23	103.0
24	107.0
25	86.0
26	68.0
27	51.0
28	25.0
29	16.0
30	16.0
31	4.0
32	0.0
33	0.0
34	0.0
35	0.0
36	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	98.33872707659116	0.5393743257820928	0.3667745415318231	0.7551240560949299
8	97.71453212591635	1.0780508840017249	0.7977576541612763	0.4096593359206554
9	97.6051779935275	0.8414239482200648	0.6472491909385114	0.9061488673139159
10	62.38947595428078	16.60556394220401	8.022428294155704	12.982531809359498
11	43.479198103039444	21.90127182582453	18.689372709635695	15.930157361500324
12	37.24137931034483	17.176724137931036	24.116379310344826	21.465517241379313
13	28.23884457857297	18.473809010562622	26.384996766544518	26.902349644319894
14	19.20258620689655	26.22844827586207	31.831896551724135	22.737068965517242
15	26.551724137931032	23.53448275862069	30.323275862068964	19.59051724137931
16	32.2198275862069	23.987068965517242	26.594827586206897	17.198275862068964
17	26.951272100043123	23.35058214747736	31.58689090125054	18.111254851228978
18	28.663793103448278	23.426724137931036	29.52586206896552	18.38362068965517
19	25.74385510996119	28.978007761966364	26.002587322121606	19.27554980595084
20	27.93103448275862	26.961206896551726	29.54741379310345	15.560344827586206
21	24.741379310344826	27.133620689655174	28.728448275862068	19.396551724137932
22	21.573275862068968	24.741379310344826	33.793103448275865	19.892241379310345
23	23.18809318377912	25.81967213114754	31.190681622088007	19.801553062985334
24	24.63362068965517	26.142241379310345	28.749999999999996	20.474137931034484
25	24.439171699741156	28.30025884383089	24.67644521138913	22.584124245038826
26	24.352331606217618	27.849740932642487	27.763385146804836	20.03454231433506
27	21.427027027027027	25.945945945945947	25.6	27.027027027027028
28	20.081967213114755	29.24935289042278	26.919758412424503	23.748921484037965
29	26.986183074265973	29.20984455958549	22.81951640759931	20.984455958549223
30	24.557999137559293	26.26131953428202	29.193617938766707	19.987063389391977
31	28.23884457857297	26.21254580728605	27.117913343392974	18.430696270748008
32	30.77753779697624	25.658747300215985	24.85961123110151	18.704103671706264
33	28.626859236904505	26.384996766544518	25.285621901271828	19.702522095279154
34	23.728448275862068	26.03448275862069	31.939655172413794	18.29741379310345
35	19.486958396206077	31.10584177624488	31.83875835309334	17.5684414744557
36	19.974087670049663	32.066508313539195	27.87734830490175	20.082055711509394
37	23.88445785729683	30.00646691097219	26.816124164690663	19.29295106704031
38	23.426724137931036	30.129310344827587	26.487068965517242	19.95689655172414
39	26.42241379310345	29.84913793103448	23.060344827586206	20.66810344827586
40	23.2377667600776	27.031687863763743	27.419702522095278	22.310842854063377
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	2.0
16	1.5
17	1.0
18	1.0
19	3.5
20	6.0
21	9.0
22	12.0
23	12.0
24	16.5
25	21.0
26	23.0
27	25.0
28	25.0
29	26.0
30	27.0
31	38.5
32	50.0
33	50.0
34	51.5
35	53.0
36	55.5
37	58.0
38	58.0
39	77.0
40	96.0
41	120.5
42	145.0
43	145.0
44	171.0
45	197.0
46	238.0
47	279.0
48	279.0
49	328.0
50	377.0
51	390.5
52	404.0
53	404.0
54	386.5
55	369.0
56	411.0
57	453.0
58	453.0
59	436.5
60	420.0
61	405.5
62	391.0
63	391.0
64	429.0
65	467.0
66	450.5
67	434.0
68	434.0
69	308.0
70	182.0
71	133.5
72	85.0
73	85.0
74	63.5
75	42.0
76	33.5
77	25.0
78	25.0
79	16.5
80	8.0
81	7.0
82	6.0
83	6.0
84	4.5
85	3.0
86	2.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.10775862068965517
8	0.04310344827586207
9	0.10775862068965517
10	0.06465517241379311
11	0.021551724137931036
12	0.0
13	0.021551724137931036
14	0.0
15	0.0
16	0.0
17	0.04310344827586207
18	0.0
19	0.04310344827586207
20	0.0
21	0.0
22	0.0
23	0.08620689655172414
24	0.0
25	0.08620689655172414
26	0.1724137931034483
27	0.3232758620689655
28	0.08620689655172414
29	0.1724137931034483
30	0.04310344827586207
31	0.021551724137931036
32	0.21551724137931033
33	0.021551724137931036
34	0.0
35	0.021551724137931036
36	0.1939655172413793
37	0.021551724137931036
38	0.0
39	0.0
40	0.021551724137931036
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	4640.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.5948275862069
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.47934295669488	80.94827586206897
2	4.28073668491787	7.413793103448276
3	1.0452961672473868	2.71551724137931
4	0.44798407167745147	1.5517241379310345
5	0.22399203583872573	0.9698275862068966
6	0.1991040318566451	1.0344827586206897
7	0.0	0.0
8	0.0	0.0
9	0.09955201592832255	0.7758620689655172
>10	0.1991040318566451	2.8663793103448274
>50	0.024888003982080638	1.7241379310344827
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	80	1.7241379310344827	No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	44	0.9482758620689655	No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA	19	0.40948275862068967	No Hit
GCCCTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	15	0.3232758620689655	No Hit
GCCCTCGGGACTAATCCTAGCCCTAGCCCTACACAAATAT	12	0.25862068965517243	No Hit
GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	12	0.25862068965517243	No Hit
GCCCTCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT	11	0.23706896551724138	No Hit
GCCCTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA	10	0.21551724137931033	No Hit
GCCCTCGGGGGTTGTGTGGCTTTGGCCCTAAGCCATTGTC	10	0.21551724137931033	No Hit
GCCCTCGGGGGGGAGGTAGTGACGAAAAATAACAATACAG	9	0.1939655172413793	No Hit
GCCCTCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA	9	0.1939655172413793	No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAG	9	0.1939655172413793	No Hit
GCCCTCGGGCGTCCCTCTTAATCATGGCCTCAGTTCCGAA	9	0.1939655172413793	No Hit
GCCCTCGGGGGGGCTGGTGAGCTGGCTCAGTGGGTAAGAG	6	0.12931034482758622	No Hit
GCCCTCGGGGGGCTGGCCAGATGGCTCAGCCAGTGAGAAC	6	0.12931034482758622	No Hit
GCCCTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6	0.12931034482758622	No Hit
GCCCTCGGGATGCCACAACTAGATACATCAACATGATTTA	6	0.12931034482758622	No Hit
GCCCTCGGGGAGTTGAAGCTGTTTGACAGCTGGCAGGGTT	6	0.12931034482758622	No Hit
GCCCTCGGGGATCCTGCCAGTAGCATATGCTTGTCTCAAA	6	0.12931034482758622	No Hit
GCCCTCGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGC	6	0.12931034482758622	No Hit
GCCCTCGGGCGGGGAGGTAGTGACGAAAAATAACAATACA	6	0.12931034482758622	No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC	5	0.10775862068965517	No Hit
GCCCTCGGGGGGGTTGGTGAGATGGCTCAGTGGGTAGGAG	5	0.10775862068965517	No Hit
GCCCTCGGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAG	5	0.10775862068965517	No Hit
GCCCTCGGGGTGTTCTTTATTAATATCCTAACACTCCTCG	5	0.10775862068965517	No Hit
GCCCTCGGGGCCTCTTGTCCGCGTGTCCTCCATTAAATTA	5	0.10775862068965517	No Hit
GCCCTCGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGT	5	0.10775862068965517	No Hit
GCCCTCGGGATGGCCTACCCATTCCAACTTGGTCTACAAG	5	0.10775862068965517	No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGC	5	0.10775862068965517	No Hit
GCCCTCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC	5	0.10775862068965517	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGGGG	85	0.0	33.869568	6
CCTCGGG	450	0.0	33.869568	3
CCCTCGG	455	0.0	33.869568	2
TCGGGGT	35	8.364532E-7	33.869568	5
TCGGGGG	165	0.0	33.869568	5
TCGGGAG	35	8.364532E-7	33.869568	5
CGGGGTG	25	1.7719211E-4	33.869564	6
CGGGGGT	30	1.2208155E-5	33.869564	6
CGGGGGA	20	0.0025592274	33.869564	6
CGGGTCA	20	0.0025592274	33.869564	6
CTCGGGT	30	1.2208155E-5	33.869564	4
CTCGGGG	300	0.0	33.869564	4
CTCGGGC	45	3.8489816E-9	33.869564	4
CTCGGGA	75	0.0	33.869564	4
TCTTCTG	20	0.0025592274	33.869564	20
TCGGGTC	20	0.0025592274	33.869564	5
TCGGGGC	25	1.7719211E-4	33.869564	5
TCGGGGA	75	0.0	33.869564	5
TCGGGCG	20	0.0025592274	33.869564	5
TCGGGAA	25	1.7719211E-4	33.869564	5
>>END_MODULE