FastQCFastQC Report
Fri 17 Jun 2016
SRR1525802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99459
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG5110.5137795473511698No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3170.3187242984546396No Hit
AAGCGGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT2590.2604088116711409No Hit
AAGCGGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT2140.21516403744256427No Hit
AAGCGGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC1750.17595189977779788No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1590.15986486894097066No Hit
AAGCGGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC1460.1467941563860485No Hit
AAGCGGGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC1400.1407615198222383No Hit
AAGCGGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC1380.1387506409676349No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1260.12668536784001447No Hit
AAGCGGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1240.12467448898541107No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC1210.12165817070350596No Hit
AAGCGGGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT1190.11964729184890256No Hit
AAGCGGGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT1120.11260921585779064No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1110.11160377643048894No Hit
AAGCGGGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG1060.10657657929398043No Hit
AAGCGGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA1030.10356026101207533No Hit
AAGCGGGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG1020.10255482158477362No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGA150.00802098150.59979637
GACGACA150.00808612950.49642636
ACAAGCC150.0081188550.444935
TATTTGA150.00818458850.3421642
ATGTTCC150.00840097150.0111314
GGTGTAC204.202384E-449.9101459
GCGACAA150.00853622849.8095719
CGTGCCA150.00853622849.8095719
AGGCGAC150.00853622849.8095717
CGACAAG150.00855324949.78449220
GGGTGTA204.286859E-449.7094048
CGGGGTA204.286859E-449.7094044
GGATATC150.00860446149.70948
AAGCGGG99050.049.5086561
AGCGGGG97900.049.4301342
GCGGGGG92950.049.415263
CGGGGGA27700.049.1710364
CGGGGGG51150.048.3488354
CTGGTTG1250.047.93794613
CGGGGGC8250.047.6005174
Produced by FastQC (version 0.11.5)