##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1525802_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 99459 Sequences flagged as poor quality 0 Sequence length 56 %GC 55 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.13780552790597 38.0 36.0 39.0 32.0 40.0 2 35.568827355995936 38.0 36.0 39.0 30.0 40.0 3 34.16398717059291 38.0 34.0 39.0 23.0 40.0 4 34.461245337274654 38.0 35.0 39.0 23.0 40.0 5 34.16319287344534 38.0 34.0 39.0 22.0 40.0 6 34.76428478066339 38.0 35.0 39.0 25.0 40.0 7 35.28916437929197 38.0 36.0 39.0 28.0 40.0 8 34.16382630028454 38.0 35.0 39.0 25.0 40.0 9 34.15296755446968 38.0 35.0 39.0 24.0 40.0 10 33.529645381513994 37.0 32.0 39.0 22.0 40.0 11 33.581586382328396 37.0 32.0 39.0 24.0 40.0 12 32.789340331191745 37.0 32.0 39.0 17.0 40.0 13 32.54852753395872 37.0 31.0 39.0 16.0 40.0 14 33.175599995978246 38.0 32.0 39.0 19.0 40.0 15 32.2571813511095 37.0 31.0 39.0 13.0 40.0 16 31.52080756894801 36.0 29.0 38.0 15.0 40.0 17 30.6775354668758 35.0 27.0 38.0 12.0 40.0 18 29.93162006454921 35.0 25.0 38.0 11.0 40.0 19 31.22205129751958 35.0 28.0 38.0 15.0 40.0 20 31.55860203702028 36.0 29.0 38.0 17.0 40.0 21 31.723081872932564 36.0 29.0 39.0 15.0 40.0 22 31.04672277018671 36.0 27.0 39.0 12.0 40.0 23 31.631667320202293 36.0 29.0 39.0 15.0 40.0 24 31.171598347057582 36.0 28.0 39.0 13.0 40.0 25 31.454076554157997 36.0 29.0 38.0 14.0 40.0 26 32.05232306779678 36.0 31.0 39.0 13.0 40.0 27 32.06524296443761 36.0 30.0 39.0 14.0 40.0 28 32.03141998210318 36.0 30.0 39.0 14.0 40.0 29 31.63951980212952 36.0 30.0 39.0 11.0 40.0 30 31.342975497441156 36.0 29.0 39.0 10.0 40.0 31 32.06631878462482 36.0 31.0 39.0 10.0 40.0 32 31.886837792457193 36.0 31.0 39.0 7.0 40.0 33 31.708623653967965 36.0 31.0 39.0 4.0 40.0 34 31.472214681426518 36.0 30.0 38.0 4.0 40.0 35 31.277581717089454 36.0 30.0 38.0 4.0 40.0 36 30.404618988729023 35.0 29.0 38.0 4.0 39.0 37 30.133160397751837 35.0 29.0 38.0 4.0 39.0 38 29.606511225731207 35.0 27.0 38.0 4.0 39.0 39 28.82310298716054 34.0 25.0 38.0 4.0 39.0 40 26.436571853728672 33.0 15.0 38.0 4.0 39.0 41 24.91555314250093 30.0 11.0 36.0 4.0 39.0 42 25.71482721523442 31.0 16.0 36.0 4.0 39.0 43 25.495299570677364 31.0 13.0 36.0 4.0 39.0 44 24.64677907479464 30.0 10.0 36.0 4.0 39.0 45 25.384057752440704 31.0 13.0 36.0 4.0 39.0 46 23.602449250444906 28.0 4.0 35.0 4.0 39.0 47 22.5852361274495 27.0 4.0 35.0 4.0 38.0 48 23.209443087101217 28.0 4.0 35.0 4.0 38.0 49 22.193697905669673 26.0 4.0 35.0 4.0 38.0 50 22.742708050553496 27.0 4.0 35.0 4.0 38.0 51 22.847887069043527 29.0 4.0 35.0 4.0 38.0 52 22.535738344443438 28.0 4.0 35.0 4.0 38.0 53 22.333192571813512 28.0 4.0 35.0 4.0 38.0 54 21.98714042972481 28.0 4.0 35.0 4.0 38.0 55 21.59259594405735 27.0 4.0 35.0 4.0 38.0 56 21.181381272685226 26.0 4.0 35.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 3.8685040657985965 1 2 4.779354541181107 1 3 6.355922769062037 1 4 5.885260842309634 1 5 6.734476928298605 1 6 5.776414234004214 1 7 5.236256960236766 1 8 6.086447750796282 1 9 6.1028092799703195 1 10 6.387582901628182 1 11 -0.809440631412091 1 12 -6.4501106197788545 1 13 -17.70436194365537 1 14 -6.461485918142035 1 15 0.953011326620512 1 16 -0.4052519773840757 1 17 -5.348625051345444 1 18 -3.9988835436370813 1 19 0.2837547884754912 1 20 -0.08112700461311562 1 21 7.203034798308501 1 22 7.506530163652371 1 23 7.407012104186727 1 24 7.651591327248557 1 25 7.033904750338241 1 26 7.378736573978479 1 27 7.441037385966641 1 28 7.453029370292473 1 29 7.869142879315643 1 30 8.017495749915717 1 31 6.81492321905478 1 32 6.249216519715517 1 33 6.592447455573893 1 34 6.672016583864156 1 35 7.003003257919069 1 36 5.796356566856833 1 37 6.121047001066827 1 38 6.486846770724174 1 39 6.409401553847907 1 40 -6.878940497667056 1 41 1.224252021370738 1 42 0.6414295430299504 1 43 -6.326169104222405 1 44 -12.961452875654771 1 45 -0.2840696632988404 1 46 -3.456816650703807 1 47 -7.16894509597109 1 48 -2.6715322699578152 1 49 -11.041753774703135 1 50 -1.8823092336378586 1 51 4.839683344402427 1 52 4.6454201841879055 1 53 4.709588333397843 1 54 5.089088455119974 1 55 4.967372575537087 1 56 7.835944265130504 2 1 4.250455285310792 2 2 5.030411451750211 2 3 6.709174801582368 2 4 6.058268972390934 2 5 6.474314326672584 2 6 5.726170331565193 2 7 5.147395171618875 2 8 5.985390840227176 2 9 5.921914971027231 2 10 5.964168267481842 2 11 4.148282945823681 2 12 -0.4612488311609688 2 13 2.0236055360194243 2 14 5.109245789175038 2 15 5.907889375400998 2 16 5.074991925054949 2 17 7.717879013695203 2 18 5.6520920661190175 2 19 6.71603121123972 2 20 6.185620962866558 2 21 7.903360001560529 2 22 8.189700895359685 2 23 7.816036494430627 2 24 8.00980270936238 2 25 7.52488036009434 2 26 6.934671533328071 2 27 6.802094296535746 2 28 6.491891158910359 2 29 7.253207919966048 2 30 7.327983554793768 2 31 5.836467934501936 2 32 6.01840351158544 2 33 5.388545016549504 2 34 5.880390567603996 2 35 6.568938217268659 2 36 6.204161444905612 2 37 6.4989331799286205 2 38 7.135220754464012 2 39 6.875417814010504 2 40 4.530490396641888 2 41 9.644495923809764 2 42 8.421266941403935 2 43 7.708302440493043 2 44 -3.0171439325653395 2 45 7.554954726945059 2 46 7.082289040353103 2 47 4.697071164191513 2 48 7.03521569752186 2 49 -0.01500580722346001 2 50 7.3742761322157975 2 51 10.465862206191044 2 52 9.808184411830183 2 53 10.22804361795069 2 54 9.790714471380138 2 55 10.685746559276925 2 56 11.100822313910992 3 1 4.427284553603471 3 2 5.012118768823385 3 3 6.331126021094558 3 4 6.021683606537273 3 5 6.87065579008722 3 6 6.018853258394465 3 7 5.592517122838387 3 8 6.1927079133979035 3 9 6.245085702734546 3 10 6.470265828457446 3 11 5.075112214116366 3 12 5.794848729814639 3 13 6.4443372433364985 3 14 5.694611642833575 3 15 0.12740157052294876 3 16 7.33108948603056 3 17 7.80324486735374 3 18 8.956970114899505 3 19 7.7892019429470345 3 20 6.850255109208021 3 21 8.110677074731264 3 22 8.61043260267676 3 23 7.761158445650139 3 24 7.930534416679453 3 25 7.77488036009434 3 26 7.007842265035393 3 27 7.070386979462572 3 28 6.534574085739624 3 29 6.936134749234338 3 30 6.815788432842545 3 31 5.665736227184862 3 32 6.097671804268366 3 33 6.077569406793405 3 34 5.8864881285796 3 35 6.294547973366221 3 36 6.89318583514952 3 37 7.285518545782274 3 38 8.080342705683524 3 39 8.381515374986115 3 40 10.170734299080912 3 41 12.449373972590251 3 42 11.250535234086865 3 43 11.629034147810113 3 44 11.824319482068809 3 45 10.84154009279872 3 46 12.832289040353107 3 47 13.355607749557368 3 48 12.510825453619418 3 49 13.570360046435074 3 50 12.916959059045066 3 51 12.843910986678846 3 52 12.722818558171646 3 53 12.807311910633615 3 54 12.760226666502088 3 55 12.716234364154975 3 56 11.814236948057335 4 1 3.895945926263714 4 2 4.924625859010561 4 3 6.021426645031031 4 4 5.708439136883271 4 5 6.104631967058296 4 6 5.517577024701893 4 7 4.903776340081727 4 8 5.730711316687753 4 9 5.65319687686954 4 10 5.881071273721208 4 11 5.943660143781706 4 12 6.311439767818044 4 13 6.696180692003544 4 14 5.990839663253315 4 15 6.254174117318186 4 16 7.638236394708951 4 17 8.342382700592538 4 18 9.108558316828038 4 19 7.9264679667700655 4 20 7.076857491510914 4 21 7.828771232417026 4 22 8.081646442722139 4 23 7.039802801861143 4 24 8.44329675360515 4 25 7.533672192198704 4 26 6.198426496459099 4 27 6.893983122400751 4 28 6.532021618354488 4 29 6.640615747532696 4 30 7.053309703404089 4 31 6.31647360665168 4 32 5.966503341420946 4 33 6.533610246271568 4 34 5.846357669135472 4 35 6.012925738539224 4 36 6.704728659880093 4 37 6.6407369235474505 4 38 7.508164600181534 4 39 7.367618608111471 4 40 1.6348579519453459 4 41 10.591461323696322 4 42 9.923677605045452 4 43 9.982125469421003 4 44 10.86955487628321 4 45 9.66825591809651 4 46 12.394966295032628 4 47 11.910202190850615 4 48 13.11249873779412 4 49 12.312277233048803 4 50 13.029551231478418 4 51 12.804489545952816 4 52 12.191621734575502 4 53 12.85268910859164 4 54 12.278235741941678 4 55 12.401713660808408 4 56 11.691293102339806 5 1 4.344553448514645 5 2 4.950465652292216 5 3 6.441107954247226 5 4 5.87489047193926 5 5 6.752995446817124 5 6 5.937401888325205 5 7 5.08860263924911 5 8 6.0931144174629495 5 9 5.973179650340686 5 10 6.217706358418308 5 11 5.839942084637293 5 12 5.628407898739667 5 13 6.639835587208829 5 14 5.432341242351793 5 15 7.260418734027915 5 16 8.070550491751728 5 17 8.558288528901471 5 18 9.964573246486374 5 19 8.19412515884586 5 20 7.391959415139972 5 21 7.815133563740595 5 22 8.773937571059776 5 23 7.812197289371909 5 24 8.377764166754734 5 25 7.639830676264165 5 26 7.031329166571069 5 27 6.836346027941946 5 28 7.152535543131979 5 29 6.89729102746379 5 30 7.48885377460708 5 31 5.7630713672029295 5 32 5.853167136999474 5 33 6.0522005419936455 5 34 6.145596830777734 5 35 5.736583504832645 5 36 7.228702245869183 5 37 7.73289885291868 5 38 8.036229486773554 5 39 8.792611430391112 5 40 9.774392835666276 5 41 11.819066836185556 5 42 11.514762876363282 5 43 12.035312377259078 5 44 12.258300210764979 5 45 11.375189595960418 5 46 12.423677176456689 5 47 13.484635150942491 5 48 13.556368964610087 5 49 14.314789435173406 5 50 13.893246321917697 5 51 13.122893220945638 5 52 12.82369178912618 5 53 13.295267345743525 5 54 12.954520553885406 5 55 12.71947134096919 5 56 11.376191178710751 6 1 4.131609655860707 6 2 4.959437356916098 6 3 6.47849005684672 6 4 5.9740393102185365 6 5 6.755180862046018 6 6 5.840016718476271 6 7 5.369217622762648 6 8 6.054812140030236 6 9 5.82848215781711 6 10 5.685346876783459 6 11 5.934451708132421 6 12 6.7486057363288054 6 13 7.186611141230756 6 14 6.627955075646785 6 15 6.790899525378272 6 16 8.419220072305368 6 17 9.169553002484783 6 18 10.064718940834972 6 19 8.310297231539675 6 20 7.841771546111524 6 21 7.807465440130443 6 22 8.493859356198953 6 23 8.02763322219915 6 24 8.580701058097421 6 25 7.704587980151903 6 26 7.344533675427755 6 27 7.502859332136413 6 28 7.311994173605097 6 29 7.22533335550612 6 30 7.136005066685904 6 31 6.362335227336356 6 32 6.023667865471211 6 33 6.340521989735382 6 34 6.420422380965604 6 35 6.68532209849878 6 36 7.868530479900311 6 37 8.088086338540947 6 38 8.066556915651706 6 39 8.821865321963848 6 40 5.634641282871247 6 41 12.606736493420428 6 42 11.844990619634505 6 43 12.329193214618176 6 44 12.886166171964277 6 45 11.758787479558304 6 46 13.616185419689568 6 47 14.777059044815662 6 48 13.411863175176762 6 49 14.678411856766846 6 50 14.32526840611369 6 51 13.948213365106358 6 52 14.316062006134075 6 53 13.99410179095478 6 54 13.829875204602374 6 55 14.242859117980153 6 56 13.553708240285783 7 1 4.375257312551838 7 2 5.298229000055564 7 3 6.481030994170265 7 4 5.950888547937339 7 5 6.946598140419816 7 6 6.070959688549671 7 7 5.260239644219446 7 8 6.0649758893244154 7 9 6.0357097128707515 7 10 6.752258226303507 7 11 6.513503091531632 7 12 6.322010592342359 7 13 6.901179181885752 7 14 6.34760499094887 7 15 7.197167170776353 7 16 7.876306464174366 7 17 8.878474515754121 7 18 9.568389183635645 7 19 7.7241876889083905 7 20 7.576102432616324 7 21 7.828922244195944 7 22 8.067828864951075 7 23 7.5490900262646505 7 24 8.334794790452019 7 25 7.428190464623956 7 26 6.949559084800988 7 27 6.540691065620319 7 28 7.168007725270822 7 29 6.959099589272352 7 30 6.73905419147416 7 31 6.469901574033134 7 32 6.12982258032158 7 33 6.08049940362584 7 34 5.6064754583230325 7 35 6.593566028481838 7 36 7.268304618804887 7 37 7.82044094046077 7 38 7.841825125702524 7 39 8.050267354713704 7 40 7.845821407094849 7 41 11.61464163176035 7 42 10.90004426164467 7 43 11.908809250755947 7 44 12.15889344469155 7 45 10.960086180857001 7 46 12.33244741856026 7 47 12.858933691907698 7 48 11.717904959479412 7 49 12.855995143045785 7 50 12.071457000128373 7 51 11.49310325782234 7 52 11.902736201503924 7 53 11.594609978419488 7 54 11.450993217024735 7 55 11.960532748697261 7 56 10.41282738201362 8 1 4.0676932549877804 8 2 4.64115634298291 8 3 6.672589435728707 8 4 5.824089671138459 8 5 6.644386838208511 8 6 5.44073855832854 8 7 5.078509212489017 8 8 6.016537840886372 8 9 6.11407054123158 8 10 5.657312631357907 8 11 5.637406215434758 8 12 6.181961452293216 8 13 6.440052470759042 8 14 6.202027595371479 8 15 6.785443759052946 8 16 7.9077209955889 8 17 8.496059633339243 8 18 9.557332672579136 8 19 7.7809619956826985 8 20 8.005990112504001 8 21 8.069971735245439 8 22 8.562476109598315 8 23 7.701336428511048 8 24 7.900870606527835 8 25 7.207147993581483 8 26 6.9770248622667665 8 27 6.31148783641709 8 28 6.256723063986161 8 29 6.947341077513837 8 30 7.20965791207788 8 31 5.7815898857214485 8 32 5.7066039071029095 8 33 5.585150158276594 8 34 5.452737304584879 8 35 5.96939965431546 8 36 6.392032242532512 8 37 6.149065019084851 8 38 6.748108031985435 8 39 7.974626779073134 8 40 2.963582024855466 8 41 11.071819588938304 8 42 10.395393506993916 8 43 10.125902967849665 8 44 11.320529106327207 8 45 10.14971412048494 8 46 11.956877042989884 8 47 12.363847696821704 8 48 11.632611874186328 8 49 12.994552531603173 8 50 11.668771847443221 8 51 11.692295957015041 8 52 12.072357121124842 8 53 12.228867612677124 8 54 12.123773139804658 8 55 11.947282485446998 8 56 11.532430751616992 9 1 4.446685883980415 9 2 5.428099129925698 9 3 6.792719305858579 9 4 6.243096340145129 9 5 6.9985461923678685 9 6 6.285245402835386 9 7 5.468031852011656 9 8 6.19484601919455 9 9 6.2759694531304895 9 10 6.447063421108698 9 11 6.221295299323842 9 12 6.854478124809887 9 13 7.251828532535107 9 14 6.276176419520301 9 15 7.099764573373754 9 16 8.032150620018523 9 17 9.320032957312563 9 18 9.24371385896032 9 19 8.464447429168136 9 20 7.667011523525417 9 21 8.205545620819322 9 22 8.262634059756266 9 23 8.09454457171919 9 24 8.088041543698775 9 25 7.622995659429147 9 26 7.09890973415164 9 27 7.3069248318540865 9 28 7.356319413582515 9 29 7.19935932953209 9 30 7.12866458108455 9 31 5.827044431175995 9 32 6.389562840061842 9 33 6.184395507521945 9 34 5.976605328452898 9 35 6.42473485965067 9 36 7.443629294129561 9 37 7.5347266547464855 9 38 7.179487463364861 9 39 7.667150471596827 9 40 5.287379848653288 9 41 9.069187086305803 9 42 10.309135170735576 9 43 10.960757302704 9 44 11.535516821314928 9 45 10.025021245792065 9 46 12.624655210768058 9 47 13.397894730868739 9 48 12.490632232206686 9 49 12.73261851966916 9 50 12.863664792336163 9 51 12.395700660419745 9 52 12.837801136568856 9 53 13.068635952445462 9 54 13.054889320920843 9 55 13.499493787658302 9 56 12.295944265130505 10 1 4.131952341660664 10 2 4.947400518192602 10 3 6.663968746073532 10 4 6.084341302079743 10 5 6.835626353585958 10 6 5.809517682280081 10 7 5.309590293570096 10 8 6.156332808267543 10 9 6.303958705257674 10 10 6.389651867145421 10 11 6.182513391576414 10 12 6.64896983999126 10 13 6.7400058724365834 10 14 6.228169254271759 10 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6.499515951935919 114 31 5.170478774610334 114 32 5.12982258032158 114 33 4.965780788907228 114 34 5.1007034525510235 114 35 5.494720429636239 114 36 6.953730304230572 114 37 7.456804576824403 114 38 7.608058891936292 114 39 7.830209634655986 114 40 10.193584754858193 114 41 9.721423738542455 114 42 9.711732573332984 114 43 8.205346047292746 114 44 -0.33317004737194367 114 45 6.94926367003449 114 46 -0.1661095799967356 114 47 -0.19157135859735064 114 48 0.9935182350926901 114 49 -6.824380037329398 114 50 1.7215291502005243 114 51 4.468572233291315 114 52 4.22885452762225 114 53 3.762719646529156 114 54 4.18403795006947 114 55 3.817675605840119 114 56 7.351499820686062 115 1 3.1335545708490997 115 2 4.0110717128982785 115 3 4.584205597344866 115 4 4.4270790241278135 115 5 4.966800160621837 115 6 4.4042930218830065 115 7 3.9499943339741392 115 8 4.249680074028603 115 9 4.441193118354153 115 10 4.240714214759496 115 11 0.2198522978808377 115 12 -2.087080316748551 115 13 -2.6031498224432497 115 14 -5.172597029253147 115 15 -6.955069481460299 115 16 1.8236369115048134 115 17 2.7103648476444526 115 18 4.305762921009382 115 19 2.480320445041148 115 20 2.042913399427288 115 21 5.4638428791165765 115 22 6.159459456581665 115 23 4.8037797809544 115 24 5.523106478763712 115 25 5.213904750338241 115 26 4.847827483069388 115 27 4.2145727395019925 115 28 4.863534420797517 115 29 4.375405505578268 115 30 4.943960396380362 115 31 4.372498976630542 115 32 4.594469044968044 115 33 4.20820503133147 115 34 4.878481230328802 115 35 4.34320527812109 115 36 6.196154546654814 115 37 5.578016698036528 115 38 6.072705356582755 115 39 6.3857651902115435 115 40 8.042069603343045 115 41 8.12546414258286 115 42 7.055166916767323 115 43 6.114436956383656 115 44 -0.131149845351743 115 45 6.050273771044594 115 46 1.5005570866699287 115 47 -8.221874388900382 115 48 -0.18829994672549333 115 49 -3.804178017127379 115 50 1.499306927978303 115 51 2.1251378898569726 115 52 2.3803696791373987 115 53 1.9748408586503672 115 54 2.0426238086553283 115 55 2.8984836866481984 115 56 4.563621032807273 116 1 4.0417272062944605 116 2 4.8448641830543835 116 3 6.03415968366259 116 4 5.250770484182908 116 5 6.6242840908330365 116 6 5.591620845574468 116 7 5.182317566297371 116 8 5.210194307270108 116 9 5.649641235893181 116 10 5.45650852146289 116 11 3.2933141895245512 116 12 3.3013494353175616 116 13 4.699880480587055 116 14 4.971571933097632 116 15 -3.659385367594364 116 16 3.7942521548473316 116 17 6.0372702654589645 116 18 4.80805860512325 116 19 5.655710711340507 116 20 5.534189799794596 116 21 6.0083791509255775 116 22 6.932645866131708 116 23 6.093954252947057 116 24 6.886742842400071 116 25 6.22216921314816 116 26 6.101271009240186 116 27 5.040100746848186 116 28 5.611927441917818 116 29 5.437848113475422 116 30 5.809892444130597 116 31 4.843573356795826 116 32 4.76618621668522 116 33 4.558067290284637 116 34 4.667278291853137 116 35 4.667355874998954 116 36 5.786604500741131 116 37 5.531184742113659 116 38 6.525414263837117 116 39 7.562073730156449 116 40 9.596706885253056 116 41 8.227392517238506 116 42 5.579501168374303 116 43 1.7700843393037644 116 44 3.1553515320587273 116 45 4.913450997858185 116 46 -0.5122987443677189 116 47 -5.570818374208004 116 48 0.22675974106146768 116 49 -2.7738749868243495 116 50 0.21372382421337832 116 51 3.2132921598294253 116 52 3.7256405698628363 116 53 3.460607617144401 116 54 3.7662234413458684 116 55 3.503626019063258 116 56 6.291812033725545 117 1 3.219413156707688 117 2 4.05147575330232 117 3 4.8430439811832535 117 4 4.514200236249025 117 5 5.2306890495107226 117 6 4.105050597640584 117 7 3.500499384479191 117 8 3.8620538114023404 117 9 3.462657764818804 117 10 4.257128356173634 117 11 -2.148834570806031 117 12 -10.621171225839461 117 13 -10.561483155776585 117 14 -3.33421319086931 117 15 -8.578806855197673 117 16 -9.30388834102044 117 17 -0.5939280816484747 117 18 -4.0338835436370815 117 19 -0.35048763576693176 117 20 -1.0391573076434177 117 21 4.811065101338798 117 22 6.0369847091069175 117 23 4.3088302860049055 117 24 4.989015569672798 117 25 5.020722932156421 117 26 4.923585058826966 117 27 5.186794961724214 117 28 4.638786946050043 117 29 5.412021667194427 117 30 5.388404840824812 117 31 4.815680794812355 117 32 4.300277125776127 117 33 4.996083819210259 117 34 4.989592341439916 117 35 4.921488106403913 117 36 6.704992930493201 117 37 7.081804576824403 117 38 7.619422528299932 117 39 7.821371250817606 117 40 9.54712010839355 117 41 6.458797475916192 117 42 5.541278027878434 117 43 -4.586320619373922 117 44 -1.271301360503255 117 45 4.831839427610248 117 46 -2.3201499840371405 117 47 -1.6069753990013922 117 48 -0.7804716638972096 117 49 -3.671602259551621 117 50 -0.41230923363785976 117 51 3.9395318292509103 117 52 3.949814123581845 117 53 3.567012575822087 117 54 4.3633308793623975 117 55 3.647221060385572 117 56 6.625489719675958 118 1 3.4163828536773835 118 2 4.112081813908382 118 3 5.2559227690620425 118 4 4.896775993824782 118 5 5.598113291934965 118 6 4.707323324913311 118 7 4.364135748115551 118 8 3.9870538114023404 118 9 3.648263825424859 118 10 4.548795022840306 118 11 -1.4215618435333042 118 12 -8.55677728644552 118 13 -8.254664973958402 118 14 -9.440273796929915 118 15 -9.17729170368252 118 16 -7.45919137132347 118 17 0.2356173728969786 118 18 -4.204338089091628 118 19 -0.7216997569790529 118 20 -0.10355124703735541 118 21 5.958792374066071 118 22 6.957439254561464 118 23 6.157315134489757 118 24 7.159470115127345 118 25 6.426025962459452 118 26 6.059948695190599 118 27 5.709522234451487 118 28 5.797877855140957 118 29 6.112779242952005 118 30 6.433859386279352 118 31 4.266438370569933 118 32 4.584368034867033 118 33 4.39002321314965 118 34 4.701713553561127 118 35 3.474518409434218 118 36 5.579992930493201 118 37 6.078016698036528 118 38 6.229271013148416 118 39 7.567583372029723 118 40 9.168332229605667 118 41 6.1860702031889225 118 42 6.6814295430299495 118 43 -0.03707819513149602 118 44 -0.20690742110931737 118 45 6.994718215489037 118 46 0.41469850081134396 118 47 -1.7319753990013922 118 48 0.32180106337551706 118 49 -0.10720832015768167 118 50 2.807387736059109 118 51 6.655440920160004 118 52 6.067238366006087 118 53 6.343527727337239 118 54 6.76484603087755 118 55 6.52979681796133 118 56 9.280792749978989 119 1 2.8726901123102238 119 2 3.6455560915687 119 3 4.701659203170568 119 4 4.330532160139086 119 5 5.029050571709455 119 6 4.209437489818171 119 7 3.5607530842677733 119 8 3.64350201436217 119 9 3.629941062916057 119 10 3.462466622558413 119 11 -0.5040142748229428 119 12 -7.828095115902887 119 13 -4.646842563810409 119 14 -6.433578941397851 119 15 -5.8097793710539065 119 16 -6.576879884360821 119 17 0.4237780494297496 119 18 -7.007720752939406 119 19 -0.25376459136947105 119 20 -0.9115146015123408 119 21 4.456678209161211 119 22 5.3200185357453975 119 23 4.092128383256494 119 24 3.4374828001167756 119 25 4.702276843361499 119 26 5.861217194133516 119 27 5.295145913098427 119 28 5.34016115323821 119 29 5.861545980090835 119 30 5.867263191776182 119 31 4.990892211302846 119 32 5.053712643746529 119 33 4.876633502085522 119 34 5.60798557611222 119 35 5.338507133888058 119 36 7.379147264531252 119 37 7.175620644477682 119 38 7.8744436699489775 119 39 7.5711069802044975 119 40 9.204625393805813 119 41 5.748438067882368 119 42 6.196933418998942 119 43 -2.9422931352301553 119 44 -0.5490497748795775 119 45 3.708953592515112 119 46 -1.3957313793859782 119 47 -2.1421233905447323 119 48 -5.015408238950064 119 49 -3.2284204413698028 119 50 0.7911791384551634 119 51 4.8510011738597925 119 52 5.714877548528989 119 53 6.224782131847455 119 54 5.522421788453308 119 55 5.674969474761895 119 56 8.133153567456088 120 1 3.042297169246872 120 2 3.9887798285374316 120 3 5.238681389751694 120 4 4.46365164690733 120 5 5.516086123700902 120 6 4.823310785728353 120 7 4.013038569432169 120 8 4.110355796773291 120 9 4.212004682269171 120 10 4.732180602777607 120 11 -1.6979463785385285 120 12 -9.286662343916785 120 13 -7.666890679287555 120 14 -6.636198561820198 120 15 0.04841362547108474 120 16 -7.548010598073731 120 17 -0.2690848214603889 120 18 -4.825550210303749 120 19 -1.245210728765887 120 20 -0.568023556337252 120 21 2.5554485914119454 120 22 4.625150853307545 120 23 4.287931644416609 120 24 3.5784878789726946 120 25 3.6276978537865183 120 26 4.410000941794571 120 27 4.20586497217354 120 28 4.583144312821208 120 29 4.197418741384606 120 30 4.2055417269272155 120 31 3.7528542535375387 120 32 2.7818601978764406 120 33 2.9312980302865377 120 34 3.622821181565307 120 35 2.943692913091482 120 36 4.273827831224647 120 37 3.6511619435955645 120 38 3.5328237822184256 120 39 4.025263622813423 120 40 3.722668697735241 120 41 -7.558966369433859 120 42 -7.008225629383842 120 43 -9.348467954797117 120 44 -14.448809197493851 120 45 -8.832345525367808 120 46 -11.432218949554382 120 47 -9.158830153442354 120 48 -9.040267902141725 120 49 -13.205431935622677 120 50 -8.328975900304526 120 51 -5.08698332226424 120 52 -4.942166022708646 120 53 -4.883744999935489 120 54 -4.448842579362784 120 55 -4.189638918715785 120 56 -2.1235959647545535 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 4828.0 5 487.0 6 107.0 7 200.0 8 511.0 9 513.0 10 408.0 11 353.0 12 332.0 13 351.0 14 372.0 15 449.0 16 630.0 17 809.0 18 902.0 19 1258.0 20 1461.0 21 1724.0 22 1995.0 23 2385.0 24 2564.0 25 3040.0 26 3013.0 27 3223.0 28 3408.0 29 3939.0 30 4307.0 31 4716.0 32 5209.0 33 5765.0 34 6326.0 35 6864.0 36 7377.0 37 7614.0 38 6853.0 39 5166.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 99.1674961541942 0.7299490242210358 0.06635900220191235 0.036195819382861275 8 99.06795765089133 0.61432349008134 0.1880171729054183 0.12970168612191957 9 94.22676680843362 3.1912647422556026 1.5594365517449402 1.022531897565831 10 52.07371881880975 28.661056314662325 8.681969454750199 10.583255411777717 11 37.22740023527283 22.56809338521401 21.94874269799616 18.255763681517006 12 31.66631476286711 15.544093546084317 23.44282568696649 29.346766004082088 13 25.909168602137566 15.805507797182758 25.747292854341993 32.53803074633769 14 24.05212197991132 22.55200635437718 27.783307694627936 25.612563971083564 15 28.309165084622528 20.017570788059942 25.67758613725411 25.995677990063413 16 33.10566128837565 22.193497766869193 23.5102402124492 21.19060073230596 17 28.738221422178423 21.650459075413067 27.00851778477257 22.602801717635938 18 25.865934706763593 21.66621421892438 25.680933852140075 26.78691722217195 19 27.589848818612513 24.677871993723407 22.713419234135007 25.018859953529077 20 28.925220472758024 23.790923368098902 25.092618692868363 22.191237466274714 21 26.94604757787207 22.00325765649822 26.545878662349935 24.504816103279776 22 23.511195568023005 21.527463577956745 29.1788576197227 25.782483234297548 23 23.622799344453494 22.48062015503876 27.46156707789139 26.435013422616354 24 24.237579560194263 23.14560646737655 26.320975737282936 26.295838235146253 25 26.472126136272223 25.244348805405842 23.629434478320327 24.654090580001608 26 26.229277321744725 24.571463061323033 23.39851923386448 25.80074038306776 27 22.803275719833398 23.411939878065958 24.366687458500174 29.418096943600474 28 24.648814970487383 24.851934157206205 23.79108889983811 26.708161972468304 29 29.72719857406121 23.638762172341217 22.253104135826714 24.380935117770864 30 28.807557268035534 21.44545829518818 26.254263035583143 23.492721401193148 31 30.040026550274558 24.221091377194924 23.71723957600016 22.02164249653036 32 31.259616835118422 22.695228038417056 23.628501030824157 22.416654095640368 33 29.032907518698597 22.987950351825567 25.07474405822487 22.90439807125097 34 25.09419516028289 25.50119884749451 28.162841772279425 21.241764219943178 35 24.44688371718311 26.65330661322645 28.207168105054333 20.692641564536107 36 26.636219141701705 26.87463533388327 24.580005231072573 21.909140293342453 37 27.69615001158118 25.505795627347705 24.550096174181007 22.2479581868901 38 28.093035442553234 26.612651288407857 23.21754290493898 22.07677036409993 39 27.63457317195964 24.831343013069656 22.892039390216887 24.642044424753813 40 22.677695333090053 19.706645582451447 23.160199489113246 34.45545959534525 41 18.553852093429647 17.728875207006208 20.678269174108728 43.03900352545541 42 23.630972790899374 25.53097077391638 24.513402852013957 26.324653583170292 43 21.229962697852606 21.031353967133786 26.7718520012098 30.966831333803814 44 23.80442685192665 22.597746183051942 24.39312545439858 29.204701510622826 45 25.382940986778458 23.62443566591422 26.029909706546277 24.962713640761045 46 22.701416064872713 26.189131399523944 25.59406140315488 25.51539113244846 47 19.74136189129117 22.159022024651446 26.118407759143263 31.981208324914125 48 24.752206244905764 23.15123217645934 27.993398874991193 24.103162703643697 49 22.99449607870809 23.157799237918592 24.963206386968004 28.884498296405315 50 23.972029944886096 23.350361212707433 27.70607260526554 24.971536237140928 51 22.346757596545743 24.170735644243575 29.032323150801016 24.450183608409667 52 22.569888681811314 24.320757568125725 28.117664836548634 24.99168891351433 53 23.445299343531616 22.47320177075035 29.177044783042742 24.904454102675285 54 23.458557187351953 23.481740567892025 28.49237468374845 24.56732756100757 55 22.42730491088989 23.448011538422747 27.77492006818159 26.34976348250577 56 23.66895522689008 23.32819163415298 28.456784522477292 24.546068616479648 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.5 9 3.0 10 13.0 11 23.0 12 30.5 13 58.0 14 78.0 15 100.5 16 123.0 17 124.5 18 126.0 19 146.5 20 167.0 21 169.5 22 179.0 23 186.0 24 191.5 25 197.0 26 215.0 27 233.0 28 276.5 29 380.0 30 440.0 31 498.0 32 556.0 33 656.5 34 757.0 35 887.0 36 1017.0 37 1212.0 38 1516.5 39 1626.0 40 1806.0 41 1986.0 42 2187.0 43 2388.0 44 2688.5 45 2989.0 46 3498.0 47 4917.0 48 5827.0 49 5842.5 50 5858.0 51 5673.0 52 5488.0 53 5705.0 54 5971.0 55 6020.0 56 6425.0 57 6830.0 58 6835.5 59 6841.0 60 6984.5 61 7128.0 62 7311.0 63 6934.0 64 6374.0 65 5604.5 66 4835.0 67 4277.0 68 3719.0 69 3142.5 70 2566.0 71 2282.0 72 1671.5 73 1345.0 74 1188.0 75 1031.0 76 748.5 77 466.0 78 367.5 79 251.0 80 233.0 81 251.5 82 270.0 83 175.5 84 81.0 85 49.0 86 17.0 87 11.0 88 3.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.43334439316703366 16 0.04725565308318 17 0.020108788546034043 18 0.0 19 0.04222845594667149 20 0.1276908072673162 21 0.0010054394273017022 22 0.0 23 0.0 24 0.006032636563810213 25 0.011059833700318725 26 0.05127741079238681 27 0.06133180506540383 28 0.010054394273017022 29 0.15684855065906553 30 0.058315486783498724 31 0.025135985682542553 32 0.024130546255240852 33 0.12065273127620427 34 0.19807156717843535 35 0.15885942951366894 36 0.053288289646990214 37 0.16087030836827235 38 0.2292401894247881 39 0.14578871695874682 40 0.8113896178324738 41 1.0376134889753565 42 0.3026372676178124 43 0.2704632059441579 44 0.42932263545782684 45 0.2282347499974864 46 0.3126916618908294 47 0.4816054856775154 48 0.08244603303873958 49 0.2583979328165375 50 0.21114227973335745 51 0.3368222081460702 52 0.19505524889653023 53 0.293588312772097 54 0.25135985682542555 55 0.31369710131813106 56 0.2714686453714596 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 99459.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.05948179651917 #Duplication Level Percentage of deduplicated Percentage of total 1 89.50798434856176 68.07930906202556 2 6.596340947546531 10.034285484470988 3 1.7145198815566838 3.9121648116309236 4 0.6635998307952622 2.018922370021818 5 0.3952516920473773 1.5031319438160449 6 0.23001269035532995 1.049678762102977 7 0.1692047377326565 0.9008737268623251 8 0.12293781725888325 0.7480469339124665 9 0.08592428087986465 0.5881820649714958 >10 0.4626692047377327 6.598698961381072 >50 0.027760152284263963 1.4669361244331836 >100 0.022472504230118442 2.585990207019978 >500 0.001321912013536379 0.5137795473511698 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 511 0.5137795473511698 No Hit AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 317 0.3187242984546396 No Hit AAGCGGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 259 0.2604088116711409 No Hit AAGCGGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 214 0.21516403744256427 No Hit AAGCGGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 175 0.17595189977779788 No Hit AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 159 0.15986486894097066 No Hit AAGCGGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 146 0.1467941563860485 No Hit AAGCGGGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC 140 0.1407615198222383 No Hit AAGCGGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 138 0.1387506409676349 No Hit AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 126 0.12668536784001447 No Hit AAGCGGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 124 0.12467448898541107 No Hit AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 121 0.12165817070350596 No Hit AAGCGGGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 119 0.11964729184890256 No Hit AAGCGGGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT 112 0.11260921585779064 No Hit AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 111 0.11160377643048894 No Hit AAGCGGGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG 106 0.10657657929398043 No Hit AAGCGGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 103 0.10356026101207533 No Hit AAGCGGGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG 102 0.10255482158477362 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0010054394273017022 0.0 0.0 0.0 0.0 10 0.0030163182819051065 0.0 0.0 0.0 0.0 11 0.0030163182819051065 0.0 0.0 0.0 0.0 12 0.0030163182819051065 0.0 0.0 0.0 0.0 13 0.005027197136508511 0.0 0.0 0.0 0.0 14 0.008043515418413617 0.0 0.0 0.0 0.0 15 0.011059833700318725 0.0 0.0 0.0 0.0 16 0.012065273127620426 0.0 0.0 0.0 0.0 17 0.01407615198222383 0.0 0.0 0.0 0.0 18 0.01407615198222383 0.0 0.0 0.0 0.0 19 0.015081591409525533 0.0 0.0 0.0 0.0 20 0.015081591409525533 0.0 0.0 0.0 0.0 21 0.015081591409525533 0.0 0.0 0.0 0.0 22 0.015081591409525533 0.0 0.0 0.0 0.0 23 0.016087030836827235 0.0 0.0 0.0 0.0 24 0.020108788546034043 0.0 0.0 0.0 0.0 25 0.02211966740063745 0.0 0.0 0.0 0.0 26 0.02211966740063745 0.0 0.0 0.0 0.0 27 0.026141425109844258 0.0 0.0 0.0 0.0 28 0.029157743391749365 0.0 0.0 0.0 0.0 29 0.03519037995555958 0.0 0.0 0.0 0.0 30 0.03619581938286128 0.0 0.0 0.0 0.0 31 0.03921213766476639 0.0 0.0 0.0 0.0 32 0.042228455946671496 0.0 0.0 0.0 0.0 33 0.043233895373973194 0.0 0.0 0.0 0.0010054394273017022 34 0.0452447742285766 0.0 0.0 0.0 0.0010054394273017022 35 0.0452447742285766 0.0 0.0 0.0 0.0010054394273017022 36 0.0452447742285766 0.0 0.0 0.0 0.0010054394273017022 37 0.047255653083180006 0.0 0.0 0.0 0.0010054394273017022 38 0.047255653083180006 0.0 0.0 0.0010054394273017022 0.0010054394273017022 39 0.048261092510481704 0.0 0.0 0.0010054394273017022 0.0010054394273017022 40 0.050271971365085114 0.0 0.0 0.0010054394273017022 0.0010054394273017022 41 0.050271971365085114 0.0 0.0 0.0010054394273017022 0.0010054394273017022 42 0.05127741079238681 0.0 0.0 0.0010054394273017022 0.0010054394273017022 43 0.055299168501593624 0.0 0.0 0.0010054394273017022 0.0010054394273017022 44 0.06133180506540383 0.0 0.0 0.0010054394273017022 0.0010054394273017022 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGACGA 15 0.008020981 50.599796 37 GACGACA 15 0.008086129 50.496426 36 ACAAGCC 15 0.00811885 50.4449 35 TATTTGA 15 0.008184588 50.34216 42 ATGTTCC 15 0.008400971 50.01113 14 GGTGTAC 20 4.202384E-4 49.910145 9 GCGACAA 15 0.008536228 49.80957 19 CGTGCCA 15 0.008536228 49.80957 19 AGGCGAC 15 0.008536228 49.80957 17 CGACAAG 15 0.008553249 49.784492 20 GGGTGTA 20 4.286859E-4 49.709404 8 CGGGGTA 20 4.286859E-4 49.709404 4 GGATATC 15 0.008604461 49.7094 8 AAGCGGG 9905 0.0 49.508656 1 AGCGGGG 9790 0.0 49.430134 2 GCGGGGG 9295 0.0 49.41526 3 CGGGGGA 2770 0.0 49.171036 4 CGGGGGG 5115 0.0 48.348835 4 CTGGTTG 125 0.0 47.937946 13 CGGGGGC 825 0.0 47.600517 4 >>END_MODULE