FastQCFastQC Report
Fri 17 Jun 2016
SRR1525801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1279
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.3909304143862392No Hit
AAGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3127443315089914No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT40.3127443315089914No Hit
AAGCGGGGGTGAGATGGACTCAAATGAATTGACGGGTACG30.23455824863174357No Hit
AAGCGGGGGGAAAAAAAACAAAAAAAAAAAACAAAAAAAA30.23455824863174357No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT30.23455824863174357No Hit
AAGCGGGGGTGAGATGGACTTAAAGGAATTGACGGGTACG30.23455824863174357No Hit
AAGCGGGGGGGAAAAAAACAAAAAAAAAAAAAAAAAAAAA30.23455824863174357No Hit
AAGCGGGGGGAAAAAAACCAAAAAAAAAAAAAAAAAAAAA30.23455824863174357No Hit
AAGCGGGGGGTTTACGTTCCGAGAAGACCTAGCGGTTGGC20.1563721657544957No Hit
AAGCGGGGGAAGCAAAAACAAAAAAAAAAAAAAAAAAAAA20.1563721657544957No Hit
AAGCGGGGGACTGTGACCCGCGGCAGCCTGAAGAAGGTTT20.1563721657544957No Hit
AAGCGGGGATGAGATGGACTCAAAGGAATTGACGGGTACG20.1563721657544957No Hit
AAGCGGGGGGTTCTGCTTTATGTTGTTAGGCAGGCCTGGT20.1563721657544957No Hit
AAGCGGAGAGTCACAGAGTCGCTTTTTTTTTTTTTTTTTT20.1563721657544957No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC20.1563721657544957No Hit
AAGCGGGGGTGAGATGGACTCAAAGGAATTGACGGGTACG20.1563721657544957No Hit
AAGCGGGGGGGGACTACACCGAGATGAACGATCTGCACTT20.1563721657544957No Hit
AAGCGGGGGCTCGTCTTAATCAGCAACATCTTTTGATACA20.1563721657544957No Hit
AAGCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCG20.1563721657544957No Hit
AAGCGGGGGGAATGAAATTATTACGTTGTTGTTTTTGTTT20.1563721657544957No Hit
AAGCGGGGGAGCCACATACAAGTCGGCCATATTAGAGAGA20.1563721657544957No Hit
AAGCGGGGGGCAGACTCAGTGCCTTACTCAGACTCCTCTC20.1563721657544957No Hit
AAGCGGGGGATGCTTCCCCAGTGTGCCGGAATTGGAATCA20.1563721657544957No Hit
AAGCGGGGGAAAAAAAAACAAAAAAAAAAAAAAAAAAAAA20.1563721657544957No Hit
AAGCGGGGGACCAAAAAACAAAAAAAAAAAAAAAAAAAAA20.1563721657544957No Hit
AAGCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTC20.1563721657544957No Hit
AAGCGGGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT20.1563721657544957No Hit
AAGCGGGGGTGCTTACACCACATGAAACCCTGTCTCTTCT20.1563721657544957No Hit
AAGCGGGGGCTCACGCAGAGTCGACTTTCTTTTTTTTTTT20.1563721657544957No Hit
AAGCGGGGGGAACGAGTAGGCTGTGAGGCTGCTGCCTTGA20.1563721657544957No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG20.1563721657544957No Hit
AAGCGGGGGCTGGGGAAGTGTTTGGCCTCCTTGGACTGTG20.1563721657544957No Hit
AAGCGGGGGAAGTTATCCTGAAACTGAAAAGTGTCTGCTG20.1563721657544957No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT20.1563721657544957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGG1000.034.03
CGGGGGG650.034.04
CGGGGGA251.0921318E-434.04
AGCGGGG1200.034.02
AAGCGGG1250.034.01
GGGGGGC250.005557636727.1999995
Produced by FastQC (version 0.11.5)