FastQCFastQC Report
Fri 17 Jun 2016
SRR1525799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4508
Sequences flagged as poor quality0
Sequence length40
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC420.9316770186335404No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT280.6211180124223602No Hit
AAGCGGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG140.3105590062111801No Hit
AAGCGGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT130.2883762200532387No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG120.26619343389529726No Hit
AAGCGGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT90.19964507542147292No Hit
AAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC60.13309671694764863No Hit
AAGCGGGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTAC60.13309671694764863No Hit
AAGCGGGGGCTGTAGCTTTGGCACGTCCAGAGTTCTTTGT60.13309671694764863No Hit
AAGCGGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT60.13309671694764863No Hit
AAGCGGGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTA50.11091393078970718No Hit
AAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA50.11091393078970718No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC50.11091393078970718No Hit
AAGCGGGGGATTCGTAGGAGTAAACATAACATTCTTCCCT50.11091393078970718No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGC50.11091393078970718No Hit
AAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11091393078970718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGG3950.034.224723
CCAGTAG251.6573918E-434.2247226
AGTAGCA200.002422740234.2247228
CGGGGGT601.8189894E-1234.224724
CGGGGGC453.416062E-934.224724
CGGGGGA1250.034.224724
GGGTACC251.6573918E-434.224727
CAGTAGC200.002422740234.2247227
GGGGGTA301.1278236E-534.224725
AGCGGGG4200.034.224722
GGGGTAC251.6573918E-434.224726
GGGGATC200.002422740234.224726
GGTACCT200.002422740234.224728
GTAGCAT200.002422740234.2247229
CGGGGGG1650.034.2247164
ATCCTGC251.7732847E-433.84444420
GTTGATC251.7732847E-433.84444416
TGGTTGA200.002562454733.84444414
GGTTGAT251.7732847E-433.84444415
GTACCTG200.002562454733.8444449
Produced by FastQC (version 0.11.5)