##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1525799_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4508
Sequences flagged as poor quality	0
Sequence length	40
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	18.01597160603372	22.0	4.0	30.0	4.0	33.0
2	13.338509316770187	4.0	4.0	26.0	4.0	31.0
3	10.11756876663709	4.0	4.0	16.0	4.0	26.0
4	10.880212954747115	4.0	4.0	18.0	4.0	28.0
5	10.282386867790594	4.0	4.0	15.0	4.0	28.0
6	9.720496894409937	4.0	4.0	12.0	4.0	27.0
7	12.154170363797693	4.0	4.0	23.0	4.0	31.0
8	10.921472937000887	4.0	4.0	21.0	4.0	27.0
9	11.025288376220054	4.0	4.0	21.0	4.0	27.0
10	10.056344276841171	4.0	4.0	18.0	4.0	27.0
11	11.014418811002662	4.0	4.0	19.0	4.0	30.0
12	11.243566992014197	4.0	4.0	21.0	4.0	31.0
13	10.868677905944987	4.0	4.0	18.0	4.0	31.0
14	11.243345164152618	4.0	4.0	20.0	4.0	32.0
15	11.082076308784384	4.0	4.0	19.0	4.0	32.0
16	9.357364685004436	4.0	4.0	10.0	4.0	28.0
17	8.903726708074535	4.0	4.0	9.0	4.0	26.0
18	8.597160603371783	4.0	4.0	6.0	4.0	26.0
19	8.581188997338066	4.0	4.0	4.0	4.0	26.0
20	8.950310559006212	4.0	4.0	4.0	4.0	27.0
21	8.020629991126885	4.0	4.0	4.0	4.0	23.0
22	7.874445430346052	4.0	4.0	4.0	4.0	23.0
23	7.947648624667258	4.0	4.0	4.0	4.0	23.0
24	7.57963620230701	4.0	4.0	4.0	4.0	21.0
25	7.860026619343389	4.0	4.0	4.0	4.0	23.0
26	8.21606033717835	4.0	4.0	4.0	4.0	26.0
27	8.25732031943212	4.0	4.0	4.0	4.0	26.0
28	8.543478260869565	4.0	4.0	4.0	4.0	27.0
29	7.71206743566992	4.0	4.0	4.0	4.0	22.0
30	7.910825199645076	4.0	4.0	4.0	4.0	23.0
31	8.777062999112689	4.0	4.0	4.0	4.0	29.0
32	8.707852706299912	4.0	4.0	4.0	4.0	29.0
33	8.655057675244011	4.0	4.0	4.0	4.0	29.0
34	8.690328305235138	4.0	4.0	4.0	4.0	30.0
35	8.68189884649512	4.0	4.0	4.0	4.0	30.0
36	8.611579414374445	4.0	4.0	4.0	4.0	30.0
37	8.615572315882876	4.0	4.0	4.0	4.0	30.0
38	8.408606921029282	4.0	4.0	4.0	4.0	30.0
39	8.297027506654835	4.0	4.0	4.0	4.0	30.0
40	8.415039929015084	4.0	4.0	4.0	4.0	31.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	17.718300843300838
1	2	24.133778008778005
1	3	9.18300787050787
1	4	9.85545483045483
1	5	22.464557177057173
1	6	1.5231285231285216
1	7	1.751471088971087
1	8	-6.34099282849283
1	9	-6.474139374139375
1	10	-5.453299178299179
1	11	-7.207423957423957
1	12	-7.565960915960915
1	13	-6.96712652962653
1	14	-6.9368056868056875
1	15	-7.146076183576184
1	16	-5.522669035169036
1	17	-5.085659048159048
1	18	-4.680109230109231
1	19	-4.99594572094572
1	20	-5.09966306216306
1	21	-4.2081973206973196
1	22	-4.137696575196575
1	23	-3.7586904086904083
1	24	-3.79756546006546
1	25	-3.5312009312009316
1	26	-3.6011742761742767
1	27	-3.634081646581647
1	28	-3.190433402933401
1	29	-2.667348567348567
1	30	-2.864265914265914
1	31	-3.5315632190632193
1	32	-3.5637177762177767
1	33	-3.582244069744071
1	34	-3.8299742674742667
1	35	-3.089736577236577
1	36	-3.131415281415282
1	37	-3.0399312024312035
1	38	-2.7411255036255033
1	39	-2.9476323976323977
1	40	-3.1471550596550584
2	1	16.051634176634174
2	2	-7.532888657888659
2	3	-5.81699212949213
2	4	-6.14454516954517
2	5	-6.202109489609489
2	6	-5.810204810204812
2	7	-7.915195577695579
2	8	-6.34099282849283
2	9	-6.474139374139375
2	10	-5.453299178299179
2	11	-7.207423957423957
2	12	-7.565960915960915
2	13	-6.96712652962653
2	14	-6.9368056868056875
2	15	-7.146076183576184
2	16	-5.522669035169036
2	17	-5.085659048159048
2	18	-4.680109230109231
2	19	-4.99594572094572
2	20	-5.09966306216306
2	21	-4.2081973206973196
2	22	-4.137696575196575
2	23	-3.7586904086904083
2	24	-3.79756546006546
2	25	-3.5312009312009316
2	26	-3.6011742761742767
2	27	-3.634081646581647
2	28	-3.190433402933401
2	29	-2.667348567348567
2	30	-2.864265914265914
2	31	-3.5315632190632193
2	32	-3.5637177762177767
2	33	-3.582244069744071
2	34	-3.8299742674742667
2	35	-3.089736577236577
2	36	-3.131415281415282
2	37	-3.0399312024312035
2	38	-2.7411255036255033
2	39	-2.9476323976323977
2	40	-3.1471550596550584
4	1	13.051634176634174
4	2	-7.532888657888659
4	3	-5.81699212949213
4	4	-6.14454516954517
4	5	-6.202109489609489
4	6	-5.810204810204812
4	7	-7.915195577695579
4	8	-6.34099282849283
4	9	-6.474139374139375
4	10	-5.453299178299179
4	11	-7.207423957423957
4	12	-7.565960915960915
4	13	-6.96712652962653
4	14	-6.9368056868056875
4	15	-7.146076183576184
4	16	-5.522669035169036
4	17	-5.085659048159048
4	18	-4.680109230109231
4	19	-4.99594572094572
4	20	-5.09966306216306
4	21	-4.2081973206973196
4	22	-4.137696575196575
4	23	-3.7586904086904083
4	24	-3.79756546006546
4	25	-3.5312009312009316
4	26	-3.6011742761742767
4	27	-3.634081646581647
4	28	-3.190433402933401
4	29	-2.667348567348567
4	30	-2.864265914265914
4	31	-3.5315632190632193
4	32	-3.5637177762177767
4	33	-3.582244069744071
4	34	-3.8299742674742667
4	35	-3.089736577236577
4	36	-3.131415281415282
4	37	-3.0399312024312035
4	38	-2.7411255036255033
4	39	-2.9476323976323977
4	40	-3.1471550596550584
5	1	6.051634176634174
5	2	5.967111342111341
5	3	9.18300787050787
5	4	10.688788163788162
5	5	8.464557177057179
5	6	12.02312852312852
5	7	4.418137755637753
5	8	7.65900717150717
5	9	9.859193959193957
5	10	6.880034155034153
5	11	11.792576042576043
5	12	8.934039084039085
5	13	11.03287347037347
5	14	12.563194313194312
5	15	9.520590483090484
5	16	-4.022669035169036
5	17	-4.252325714825715
5	18	-0.18010923010923108
5	19	-2.329279054279053
5	20	-2.266329728829727
5	21	0.6251360126360144
5	22	-2.637696575196575
5	23	-1.5920237420237413
5	24	1.035767873267874
5	25	1.4687990687990684
5	26	-2.4345076095076097
5	27	-2.134081646581647
5	28	-2.190433402933401
5	29	0.8326514326514332
5	30	-0.5309325809325811
5	31	-0.3648965523965524
5	32	1.1029488904488893
5	33	0.41775593025592883
5	34	0.33669239919239935
5	35	0.24359675609675602
5	36	2.5352513852513843
5	37	2.2934021309021304
5	38	0.25887449637449667
5	39	-0.4476323976323977
5	40	1.5195116070116077
6	1	13.513172638172634
6	2	15.928649803649801
6	3	5.3368540243540235
6	4	10.009300984300983
6	5	7.336352048852051
6	6	-0.5794355794355823
6	7	5.930958268458268
6	8	10.351314863814864
6	9	11.679706779706779
6	10	4.931316206316206
6	11	4.946422196422196
6	12	12.510962160962162
6	13	12.109796547296547
6	14	14.755502005502006
6	15	13.392385354885356
6	16	12.861946349446347
6	17	14.837417874917875
6	18	10.935275385275386
6	19	12.388669663669663
6	20	15.3618753993754
6	21	9.79180267930268
6	22	11.169995732495732
6	23	9.856694206694208
6	24	6.97166530916531
6	25	7.93033753033753
6	26	12.091133416133417
6	27	10.365918353418353
6	28	13.117258904758906
6	29	10.024959124959127
6	30	10.981887931887933
6	31	13.46843678093678
6	32	15.436282223782223
6	33	13.571602084102082
6	34	14.323871886371887
6	35	16.37180188430188
6	36	13.2532001032001
6	37	14.729299566799567
6	38	13.489643727143726
6	39	16.89852144852145
6	40	13.160537248037247
7	1	7.151634176634175
7	2	11.86711134211134
7	3	8.68300787050787
7	4	8.55545483045483
7	5	7.797890510390511
7	6	-0.21020481020481263
7	7	1.6848044223044205
7	8	4.359007171507169
7	9	5.425860625860626
7	10	5.346700821700821
7	11	0.09257604257604335
7	12	6.234039084039086
7	13	6.03287347037347
7	14	4.663194313194312
7	15	4.853923816423816
7	16	9.177330964830963
7	17	5.514340951840952
7	18	7.019890769890768
7	19	8.20405427905428
7	20	4.600336937836939
7	21	6.39180267930268
7	22	6.2623034248034255
7	23	5.741309591309592
7	24	4.002434539934541
7	25	5.868799068799069
7	26	5.598825723825723
7	27	7.865918353418353
7	28	7.1095665970666
7	29	6.532651432651432
7	30	5.7357340857340855
7	31	7.06843678093678
7	32	3.9362822237822233
7	33	3.817755930255929
7	34	3.1700257325257333
7	35	7.610263422763422
7	36	5.868584718584718
7	37	5.860068797568797
7	38	6.258874496374497
7	39	4.052367602367602
7	40	2.552844940344941
9	1	14.051634176634174
9	2	14.467111342111341
9	3	10.18300787050787
9	4	10.85545483045483
9	5	9.797890510390511
9	6	12.189795189795188
9	7	14.08480442230442
9	8	13.65900717150717
9	9	16.525860625860624
9	10	14.54670082170082
9	11	6.792576042576043
9	12	18.434039084039085
9	13	-6.96712652962653
9	14	-6.9368056868056875
9	15	-7.146076183576184
9	16	-5.522669035169036
9	17	-5.085659048159048
9	18	-4.680109230109231
9	19	-4.99594572094572
9	20	-5.09966306216306
9	21	-4.2081973206973196
9	22	-4.137696575196575
9	23	-3.7586904086904083
9	24	-3.79756546006546
9	25	-3.5312009312009316
9	26	-3.6011742761742767
9	27	-3.634081646581647
9	28	-3.190433402933401
9	29	-2.667348567348567
9	30	-2.864265914265914
9	31	-3.5315632190632193
9	32	-3.5637177762177767
9	33	-3.582244069744071
9	34	-3.8299742674742667
9	35	-3.089736577236577
9	36	-3.131415281415282
9	37	-3.0399312024312035
9	38	-2.7411255036255033
9	39	-2.9476323976323977
9	40	-3.1471550596550584
10	1	-3.5847294597294628
10	2	-3.3510704760704773
10	3	-0.7260830385830381
10	4	0.49181846681846686
10	5	-3.202109489609489
10	6	-2.6283866283866306
10	7	2.084804422304421
10	8	1.7499162624162619
10	9	2.162224262224262
10	10	2.455791730791729
10	11	5.156212406212406
10	12	4.161311811311812
10	13	4.214691652191652
10	14	2.335921585921586
10	15	5.853923816423816
10	16	3.2046036921036904
10	17	3.6416136791136786
10	18	6.501708951708951
10	19	5.00405427905428
10	20	6.354882392382395
10	21	5.609984497484499
10	22	3.5895761520761518
10	23	6.968582318582318
10	24	4.475161812661813
10	25	7.286980886980887
10	26	5.489734814734815
10	27	6.456827444327445
10	28	5.355021142521144
10	29	5.241742341742341
10	30	4.681188631188631
10	31	7.559345871845872
10	32	7.527191314691315
10	33	7.963210475710474
10	34	8.98820755070755
10	35	7.819354331854331
10	36	6.323130173130173
10	37	5.141886979386978
10	38	7.167965405465405
10	39	5.506913056913057
10	40	6.398299485799487
11	1	2.1944913194913163
11	2	7.752825627825626
11	3	1.3258650133650125
11	4	5.141169116169117
11	5	4.369319081819082
11	6	1.6183666183666165
11	7	7.9419472794472785
11	8	5.8018643143643125
11	9	6.240146340146341
11	10	6.975272250272248
11	11	3.6497188997189003
11	12	5.71975336975337
11	13	4.747159184659184
11	14	3.348908598908599
11	15	0.8539238164238157
11	16	6.3344738219738215
11	17	3.7714838089838096
11	18	5.89131934131934
11	19	4.432625707625709
11	20	5.757479794979798
11	21	2.934659822159823
11	22	4.290874853374854
11	23	7.527023877023878
11	24	6.631005968505969
11	25	9.183084783084784
11	26	9.970254295254296
11	27	3.937346924846924
11	28	9.952423739923741
11	29	2.761222861222862
11	30	3.4214483714483723
11	31	5.611293923793923
11	32	7.436282223782223
11	33	4.9891845016845
11	34	7.45574001824002
11	35	4.767406279906281
11	36	3.5828704328704317
11	37	3.245783083283083
11	38	2.1160173535173543
11	39	4.052367602367602
11	40	4.995702083202084
12	1	1.051634176634174
12	2	4.103474978474978
12	3	4.273916961416962
12	4	7.037273012273014
12	5	4.616072328572329
12	6	-0.7192957192957206
12	7	7.084804422304421
12	8	6.0226435351435335
12	9	6.253133353133352
12	10	6.092155367155366
12	11	4.156212406212406
12	12	5.252220902220902
12	13	3.3056007431007437
12	14	5.608648858648859
12	15	5.399378361878362
12	16	1.7500582375582372
12	17	2.1870682245682254
12	18	3.9562544062544056
12	19	2.2767815517815535
12	20	4.354882392382395
12	21	1.0645299520299538
12	22	-1.2286056661056657
12	23	-1.395054045054045
12	24	-0.8884745509745509
12	25	-0.6221100221100224
12	26	-1.1466288216288225
12	27	0.5477365352365346
12	28	-1.2813424938424918
12	29	1.4235605235605249
12	30	-0.22790227790227746
12	31	-0.16792685542685604
12	32	-0.018263230763230887
12	33	-0.12769861519861703
12	34	1.0791166416166416
12	35	0.6375361500361505
12	36	1.8685847185847182
12	37	1.9600687975687965
12	38	1.077056314556315
12	39	1.143276693276694
12	40	1.3073903948903967
14	1	-12.948365823365826
14	2	-7.532888657888659
14	3	-5.81699212949213
14	4	-6.14454516954517
14	5	-6.202109489609489
14	6	-5.810204810204812
14	7	-7.915195577695579
14	8	-6.34099282849283
14	9	-6.474139374139375
14	10	-5.453299178299179
14	11	-7.207423957423957
14	12	-7.565960915960915
14	13	-6.96712652962653
14	14	-6.9368056868056875
14	15	-7.146076183576184
14	16	-5.522669035169036
14	17	-5.085659048159048
14	18	-4.680109230109231
14	19	-4.99594572094572
14	20	-5.09966306216306
14	21	-4.2081973206973196
14	22	-4.137696575196575
14	23	-3.7586904086904083
14	24	-3.79756546006546
14	25	-3.5312009312009316
14	26	-3.6011742761742767
14	27	-3.634081646581647
14	28	-3.190433402933401
14	29	-2.667348567348567
14	30	-2.864265914265914
14	31	-3.5315632190632193
14	32	-3.5637177762177767
14	33	-3.582244069744071
14	34	-3.8299742674742667
14	35	-3.089736577236577
14	36	-3.131415281415282
14	37	-3.0399312024312035
14	38	-2.7411255036255033
14	39	-2.9476323976323977
14	40	-3.1471550596550584
15	1	16.051634176634174
15	2	5.467111342111341
15	3	11.18300787050787
15	4	12.85545483045483
15	5	12.797890510390511
15	6	1.1897951897951877
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109	23	-3.7586904086904083
109	24	-3.79756546006546
109	25	-3.5312009312009316
109	26	-3.6011742761742767
109	27	-3.634081646581647
109	28	-3.190433402933401
109	29	-2.667348567348567
109	30	-2.864265914265914
109	31	-3.5315632190632193
109	32	-3.5637177762177767
109	33	-3.582244069744071
109	34	-3.8299742674742667
109	35	-3.089736577236577
109	36	-3.131415281415282
109	37	-3.0399312024312035
109	38	-2.7411255036255033
109	39	-2.9476323976323977
109	40	-3.1471550596550584
110	1	-12.948365823365826
110	2	-7.532888657888659
110	3	-5.81699212949213
110	4	-6.14454516954517
110	5	-6.202109489609489
110	6	-5.810204810204812
110	7	-7.915195577695579
110	8	-6.34099282849283
110	9	-6.474139374139375
110	10	-5.453299178299179
110	11	-7.207423957423957
110	12	-7.565960915960915
110	13	-6.96712652962653
110	14	-6.9368056868056875
110	15	-7.146076183576184
110	16	-5.522669035169036
110	17	-5.085659048159048
110	18	-4.680109230109231
110	19	-4.99594572094572
110	20	-5.09966306216306
110	21	-4.2081973206973196
110	22	-4.137696575196575
110	23	-3.7586904086904083
110	24	-3.79756546006546
110	25	-3.5312009312009316
110	26	-3.6011742761742767
110	27	-3.634081646581647
110	28	-3.190433402933401
110	29	-2.667348567348567
110	30	-2.864265914265914
110	31	-3.5315632190632193
110	32	-3.5637177762177767
110	33	-3.582244069744071
110	34	-3.8299742674742667
110	35	-3.089736577236577
110	36	-3.131415281415282
110	37	-3.0399312024312035
110	38	-2.7411255036255033
110	39	-2.9476323976323977
110	40	-3.1471550596550584
111	1	1.6230627480627469
111	2	2.6099684849684834
111	3	-1.6741349866349875
111	4	-1.4302594552594563
111	5	-5.059252346752346
111	6	-2.9530619530619555
111	7	-0.4866241491241503
111	8	-0.4838499713499722
111	9	-0.33128223128223233
111	10	-0.16758489258489284
111	11	-3.064566814566815
111	12	-0.5659609159609147
111	13	-1.3956979581979585
111	14	-0.22251997251997402
111	15	-0.14607618357618435
111	16	-3.0940976065976074
111	17	-2.2285161910161913
111	18	-1.5372520872520878
111	19	-0.9959457209457199
111	20	-1.09966306216306
111	21	-4.2081973206973196
111	22	-4.137696575196575
111	23	-3.7586904086904083
111	24	-3.79756546006546
111	25	-3.5312009312009316
111	26	-3.6011742761742767
111	27	-3.634081646581647
111	28	-3.190433402933401
111	29	-2.667348567348567
111	30	-2.864265914265914
111	31	-3.5315632190632193
111	32	-3.5637177762177767
111	33	-3.582244069744071
111	34	-3.8299742674742667
111	35	-3.089736577236577
111	36	-3.131415281415282
111	37	-3.0399312024312035
111	38	-2.7411255036255033
111	39	-2.9476323976323977
111	40	-3.1471550596550584
112	1	2.384967509967506
112	2	1.1337780087780072
112	3	5.516341203841204
112	4	1.8554548304548302
112	5	8.464557177057179
112	6	-0.8102048102048123
112	7	8.751471088971089
112	8	6.325673838173838
112	9	6.8591939591939575
112	10	7.2133674883674885
112	11	5.792576042576043
112	12	9.767372417372417
112	13	0.36620680370680425
112	14	0.3965276465276464
112	15	0.18725714975714958
112	16	2.477330964830964
112	17	2.247674285174286
112	18	-0.013442563442565003
112	19	2.670720945720946
112	20	4.567003604503606
112	21	6.7918026793026804
112	22	6.862303424803425
112	23	6.241309591309592
112	24	6.20243453993454
112	25	7.1354657354657345
112	26	7.065492390492389
112	27	5.699251686751687
112	28	3.142899930399933
112	29	7.332651432651433
112	30	5.135734085734086
112	31	3.4684367809367807
112	32	3.1029488904488893
112	33	3.7510892635892628
112	34	1.8366923991923993
112	35	2.576930089430089
112	36	7.535251385251384
112	37	5.626735464235463
112	38	6.925541163041163
112	39	5.719034269034268
112	40	7.1861782736782756
115	1	-12.948365823365826
115	2	-7.532888657888659
115	3	-5.81699212949213
115	4	-6.14454516954517
115	5	-6.202109489609489
115	6	-5.810204810204812
115	7	-7.915195577695579
115	8	-6.34099282849283
115	9	-6.474139374139375
115	10	-5.453299178299179
115	11	-7.207423957423957
115	12	-7.565960915960915
115	13	-6.96712652962653
115	14	-6.9368056868056875
115	15	-7.146076183576184
115	16	-5.522669035169036
115	17	-5.085659048159048
115	18	-4.680109230109231
115	19	-4.99594572094572
115	20	-5.09966306216306
115	21	-4.2081973206973196
115	22	-4.137696575196575
115	23	-3.7586904086904083
115	24	-3.79756546006546
115	25	-3.5312009312009316
115	26	-3.6011742761742767
115	27	-3.634081646581647
115	28	-3.190433402933401
115	29	-2.667348567348567
115	30	-2.864265914265914
115	31	-3.5315632190632193
115	32	-3.5637177762177767
115	33	-3.582244069744071
115	34	-3.8299742674742667
115	35	-3.089736577236577
115	36	-3.131415281415282
115	37	-3.0399312024312035
115	38	-2.7411255036255033
115	39	-2.9476323976323977
115	40	-3.1471550596550584
116	1	-12.948365823365826
116	2	-7.532888657888659
116	3	-5.81699212949213
116	4	-6.14454516954517
116	5	-6.202109489609489
116	6	-5.810204810204812
116	7	-7.915195577695579
116	8	-6.34099282849283
116	9	-6.474139374139375
116	10	-5.453299178299179
116	11	-7.207423957423957
116	12	-7.565960915960915
116	13	-6.96712652962653
116	14	-6.9368056868056875
116	15	-7.146076183576184
116	16	-5.522669035169036
116	17	-5.085659048159048
116	18	-4.680109230109231
116	19	-4.99594572094572
116	20	-5.09966306216306
116	21	-4.2081973206973196
116	22	-4.137696575196575
116	23	-3.7586904086904083
116	24	-3.79756546006546
116	25	-3.5312009312009316
116	26	-3.6011742761742767
116	27	-3.634081646581647
116	28	-3.190433402933401
116	29	-2.667348567348567
116	30	-2.864265914265914
116	31	-3.5315632190632193
116	32	-3.5637177762177767
116	33	-3.582244069744071
116	34	-3.8299742674742667
116	35	-3.089736577236577
116	36	-3.131415281415282
116	37	-3.0399312024312035
116	38	-2.7411255036255033
116	39	-2.9476323976323977
116	40	-3.1471550596550584
117	1	-4.115032490032492
117	2	-3.199555324555325
117	3	-5.81699212949213
117	4	-6.14454516954517
117	5	-6.202109489609489
117	6	-5.810204810204812
117	7	-7.915195577695579
117	8	-6.34099282849283
117	9	-6.474139374139375
117	10	-5.453299178299179
117	11	-7.207423957423957
117	12	-7.565960915960915
117	13	-6.96712652962653
117	14	-6.9368056868056875
117	15	-7.146076183576184
117	16	-5.522669035169036
117	17	-5.085659048159048
117	18	-4.680109230109231
117	19	-4.99594572094572
117	20	-5.09966306216306
117	21	-4.2081973206973196
117	22	-4.137696575196575
117	23	-3.7586904086904083
117	24	-3.79756546006546
117	25	-3.5312009312009316
117	26	-3.6011742761742767
117	27	-3.634081646581647
117	28	-3.190433402933401
117	29	-2.667348567348567
117	30	-2.864265914265914
117	31	-3.5315632190632193
117	32	-3.5637177762177767
117	33	-3.582244069744071
117	34	-3.8299742674742667
117	35	-3.089736577236577
117	36	-3.131415281415282
117	37	-3.0399312024312035
117	38	-2.7411255036255033
117	39	-2.9476323976323977
117	40	-3.1471550596550584
118	1	-12.948365823365826
118	2	-7.532888657888659
118	3	-5.81699212949213
118	4	-6.14454516954517
118	5	-6.202109489609489
118	6	-5.810204810204812
118	7	-7.915195577695579
118	8	-6.34099282849283
118	9	-6.474139374139375
118	10	-5.453299178299179
118	11	-7.207423957423957
118	12	-7.565960915960915
118	13	-6.96712652962653
118	14	-6.9368056868056875
118	15	-7.146076183576184
118	16	-5.522669035169036
118	17	-5.085659048159048
118	18	-4.680109230109231
118	19	-4.99594572094572
118	20	-5.09966306216306
118	21	-4.2081973206973196
118	22	-4.137696575196575
118	23	-3.7586904086904083
118	24	-3.79756546006546
118	25	-3.5312009312009316
118	26	-3.6011742761742767
118	27	-3.634081646581647
118	28	-3.190433402933401
118	29	-2.667348567348567
118	30	-2.864265914265914
118	31	-3.5315632190632193
118	32	-3.5637177762177767
118	33	-3.582244069744071
118	34	-3.8299742674742667
118	35	-3.089736577236577
118	36	-3.131415281415282
118	37	-3.0399312024312035
118	38	-2.7411255036255033
118	39	-2.9476323976323977
118	40	-3.1471550596550584
119	1	-7.948365823365826
119	2	-1.8662219912219928
119	3	-3.150325462825463
119	4	-1.811211836211836
119	5	-4.202109489609489
119	6	-2.1435381435381453
119	7	-1.9151955776955791
119	8	-3.007659495159497
119	9	-3.4741393741393747
119	10	-1.7866325116325124
119	11	2.7925760425760426
119	12	-1.8992942492942486
119	13	1.6995401370401364
119	14	2.7298609798609785
119	15	2.5205904830904817
119	16	-5.522669035169036
119	17	-5.085659048159048
119	18	-4.680109230109231
119	19	-4.99594572094572
119	20	-5.09966306216306
119	21	-4.2081973206973196
119	22	-4.137696575196575
119	23	-3.7586904086904083
119	24	-3.79756546006546
119	25	-3.5312009312009316
119	26	-3.6011742761742767
119	27	-3.634081646581647
119	28	-3.190433402933401
119	29	-2.667348567348567
119	30	-2.864265914265914
119	31	-3.5315632190632193
119	32	-3.5637177762177767
119	33	-3.582244069744071
119	34	-3.8299742674742667
119	35	-3.089736577236577
119	36	-3.131415281415282
119	37	-3.0399312024312035
119	38	-2.7411255036255033
119	39	-2.9476323976323977
119	40	-3.1471550596550584
120	1	-12.948365823365826
120	2	-7.532888657888659
120	3	-5.81699212949213
120	4	-6.14454516954517
120	5	-6.202109489609489
120	6	-5.810204810204812
120	7	-7.915195577695579
120	8	-6.34099282849283
120	9	-6.474139374139375
120	10	-5.453299178299179
120	11	-7.207423957423957
120	12	-7.565960915960915
120	13	-6.96712652962653
120	14	-6.9368056868056875
120	15	-7.146076183576184
120	16	-5.522669035169036
120	17	-5.085659048159048
120	18	-4.680109230109231
120	19	-4.99594572094572
120	20	-5.09966306216306
120	21	-4.2081973206973196
120	22	-4.137696575196575
120	23	-3.7586904086904083
120	24	-3.79756546006546
120	25	-3.5312009312009316
120	26	-3.6011742761742767
120	27	-3.634081646581647
120	28	-3.190433402933401
120	29	-2.667348567348567
120	30	-2.864265914265914
120	31	-3.5315632190632193
120	32	-3.5637177762177767
120	33	-3.582244069744071
120	34	-3.8299742674742667
120	35	-3.089736577236577
120	36	-3.131415281415282
120	37	-3.0399312024312035
120	38	-2.7411255036255033
120	39	-2.9476323976323977
120	40	-3.1471550596550584
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	2549.0
5	270.0
6	40.0
7	63.0
8	39.0
9	52.0
10	92.0
11	128.0
12	91.0
13	80.0
14	53.0
15	44.0
16	30.0
17	29.0
18	22.0
19	55.0
20	114.0
21	126.0
22	129.0
23	106.0
24	100.0
25	89.0
26	69.0
27	51.0
28	31.0
29	24.0
30	15.0
31	6.0
32	8.0
33	1.0
34	1.0
35	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.91207815275311	0.7548845470692718	0.08880994671403197	0.24422735346358795
8	97.22653649877968	1.5309518526736188	0.710006656312403	0.5325049922343023
9	92.5177619893428	4.085257548845471	1.9094138543516874	1.4875666074600356
10	47.20124389160373	31.49711239449134	8.996001776988006	12.305641936916926
11	34.25022182786158	23.26974267968057	25.709849157054126	16.77018633540373
12	32.120674356699205	18.633540372670808	26.907719609582966	22.338065661047025
13	24.362103394719327	23.341468826270244	28.75526958065232	23.541158198358108
14	21.043285238623753	28.43507214206437	32.075471698113205	18.446170921198668
15	29.37000887311446	25.79858030168589	29.059449866903282	15.771960958296363
16	31.49955634427684	27.44010647737356	25.310559006211182	15.74977817213842
17	26.84117125110914	27.772848269742678	30.301685891748004	15.084294587400176
18	26.897470039946736	26.63115845539281	29.804704837993786	16.666666666666664
19	25.177619893428066	30.661634103019537	25.754884547069274	18.405861456483127
20	27.883762200532384	26.8189884649512	30.70097604259095	14.596273291925465
21	24.889086069210293	27.085181898846493	29.991126885536822	18.03460514640639
22	21.93877551020408	26.02040816326531	34.0505767524401	17.990239574090506
23	22.604259094942325	27.44010647737356	30.678793256433007	19.276841171251107
24	22.737355811889973	27.173913043478258	29.30346051464064	20.785270629991125
25	22.68086995117621	31.735463826009763	25.07767421216156	20.505992010652463
26	23.25633051976899	29.27587738782763	27.721012883163038	19.746779209240337
27	19.542018674966652	28.568252556691863	28.168074699866608	23.72165406847488
28	22.545535317636606	30.875166592625497	24.98889382496668	21.590404264771212
29	26.051167964404893	28.27586206896552	25.917686318131256	19.75528364849833
30	24.400532859680286	27.44227353463588	28.08614564831261	20.071047957371228
31	26.597160603371783	27.97249334516415	27.262644188110023	18.16770186335404
32	27.153349655018914	26.708212775428443	25.840195860227023	20.29824170932562
33	25.09429775904149	27.446194808076324	28.910583536720658	18.548923896161526
34	21.251109139307896	30.124223602484474	31.255545696539482	17.36912156166815
35	18.83318544809228	33.22981366459628	31.366459627329192	16.570541259982257
36	19.4179071317485	33.23705843145967	28.082648300377695	19.26238613641413
37	23.203194321206745	31.18899733806566	26.86335403726708	18.744454303460515
38	21.078322609274462	31.706234745950745	27.71244730419348	19.502995340581318
39	25.088731144631765	29.458740017746226	26.26441881100266	19.18811002661934
40	21.11801242236025	28.77107364685004	28.615794143744456	21.49511978704525
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	3.0
15	5.0
16	8.5
17	12.0
18	12.0
19	12.0
20	12.0
21	20.5
22	29.0
23	29.0
24	27.5
25	26.0
26	31.0
27	36.0
28	36.0
29	49.0
30	62.0
31	66.5
32	71.0
33	71.0
34	85.0
35	99.0
36	129.5
37	160.0
38	160.0
39	170.0
40	180.0
41	218.5
42	257.0
43	257.0
44	298.5
45	340.0
46	350.5
47	361.0
48	361.0
49	403.5
50	446.0
51	424.0
52	402.0
53	402.0
54	409.5
55	417.0
56	424.0
57	431.0
58	431.0
59	396.5
60	362.0
61	357.5
62	353.0
63	353.0
64	303.0
65	253.0
66	179.5
67	106.0
68	106.0
69	81.0
70	56.0
71	37.0
72	18.0
73	18.0
74	14.5
75	11.0
76	5.5
77	0.0
78	0.0
79	0.5
80	1.0
81	1.0
82	1.0
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.08873114463176575
8	0.022182786157941437
9	0.08873114463176575
10	0.13309671694764863
11	0.0
12	0.0
13	0.022182786157941437
14	0.06654835847382432
15	0.0
16	0.0
17	0.0
18	0.044365572315882874
19	0.08873114463176575
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.044365572315882874
26	0.13309671694764863
27	0.22182786157941436
28	0.13309671694764863
29	0.2883762200532387
30	0.08873114463176575
31	0.0
32	0.33274179236912155
33	0.022182786157941437
34	0.0
35	0.0
36	0.15527950310559005
37	0.0
38	0.022182786157941437
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	4508.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.88110026619344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.43698696281989	87.6885536823425
2	3.476581361661033	6.388642413487133
3	0.5070014485755674	1.3975155279503106
4	0.1931434089811685	0.709849157054126
5	0.14485755673587639	0.6654835847382431
6	0.09657170449058425	0.5323868677905945
7	0.0	0.0
8	0.0	0.0
9	0.024142926122646062	0.19964507542147292
>10	0.12071463061323032	2.417923691215617
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	42	0.9316770186335404	No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	28	0.6211180124223602	No Hit
AAGCGGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG	14	0.3105590062111801	No Hit
AAGCGGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT	13	0.2883762200532387	No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	12	0.26619343389529726	No Hit
AAGCGGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT	9	0.19964507542147292	No Hit
AAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC	6	0.13309671694764863	No Hit
AAGCGGGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTAC	6	0.13309671694764863	No Hit
AAGCGGGGGCTGTAGCTTTGGCACGTCCAGAGTTCTTTGT	6	0.13309671694764863	No Hit
AAGCGGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT	6	0.13309671694764863	No Hit
AAGCGGGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTA	5	0.11091393078970718	No Hit
AAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA	5	0.11091393078970718	No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC	5	0.11091393078970718	No Hit
AAGCGGGGGATTCGTAGGAGTAAACATAACATTCTTCCCT	5	0.11091393078970718	No Hit
AAGCGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGC	5	0.11091393078970718	No Hit
AAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.11091393078970718	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGGGG	395	0.0	34.22472	3
CCAGTAG	25	1.6573918E-4	34.22472	26
AGTAGCA	20	0.0024227402	34.22472	28
CGGGGGT	60	1.8189894E-12	34.22472	4
CGGGGGC	45	3.416062E-9	34.22472	4
CGGGGGA	125	0.0	34.22472	4
GGGTACC	25	1.6573918E-4	34.22472	7
CAGTAGC	20	0.0024227402	34.22472	27
GGGGGTA	30	1.1278236E-5	34.22472	5
AGCGGGG	420	0.0	34.22472	2
GGGGTAC	25	1.6573918E-4	34.22472	6
GGGGATC	20	0.0024227402	34.22472	6
GGTACCT	20	0.0024227402	34.22472	8
GTAGCAT	20	0.0024227402	34.22472	29
CGGGGGG	165	0.0	34.224716	4
ATCCTGC	25	1.7732847E-4	33.844444	20
GTTGATC	25	1.7732847E-4	33.844444	16
TGGTTGA	20	0.0025624547	33.844444	14
GGTTGAT	25	1.7732847E-4	33.844444	15
GTACCTG	20	0.0025624547	33.844444	9
>>END_MODULE