FastQCFastQC Report
Fri 17 Jun 2016
SRR1525798_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1525798_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6796
Sequences flagged as poor quality0
Sequence length56
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT2994.3996468510888755No Hit
TTTAGGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC2513.6933490288404944No Hit
TTTAGGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC821.2065921130076516No Hit
TTTAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC801.1771630370806356No Hit
TTTAGGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT711.0447321954090643No Hit
TTTAGGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA711.0447321954090643No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.6768687463213655No Hit
TTTAGGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC320.47086521483225424No Hit
TTTAGGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC260.38257798705120655No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA230.33843437316068276No Hit
TTTAGGGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC200.2942907592701589No Hit
TTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.2942907592701589No Hit
TTTAGGGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC180.26486168334314303No Hit
TTTAGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT180.26486168334314303No Hit
TTTAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAAA170.25014714537963506No Hit
TTTAGGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT170.25014714537963506No Hit
TTTAGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATG160.23543260741612712No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA140.20600353148911124No Hit
TTTAGGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAGAT140.20600353148911124No Hit
TTTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA130.19128899352560327No Hit
TTTAGGGGGTCCCCCCTCCTCTTCTGAGATGAGTTTTTGTTCCCTCCGCCCAATCT110.1618599175985874No Hit
TTTAGGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAAAAA110.1618599175985874No Hit
TTTAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.1618599175985874No Hit
TTTAGGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAAACT100.14714537963507945No Hit
TTTAGGGGGGATTGAACAAAAACTCATCTCAGAAGAGGATCTGTGACCAAAAAAAA100.14714537963507945No Hit
TTTAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCA100.14714537963507945No Hit
TTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA90.13243084167157151No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGTCACAGATCCTCTTCTGAAGGAGATT90.13243084167157151No Hit
TTTAGGGGGTCACACATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT90.13243084167157151No Hit
TTTAGGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTA90.13243084167157151No Hit
TTTAGGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGATCAATCCGCCCAATCT80.11771630370806356No Hit
TTTAGGAGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT70.10300176574455562No Hit
TTTAGGGGGTCACAGATCCTCTTCTGAGATGAGGATCGGAAGATCTCGTATGCCGT70.10300176574455562Illumina Single End Adapter 1 (95% over 23bp)
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA70.10300176574455562No Hit
TTTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA70.10300176574455562No Hit
TTTAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGCTTGAACAAAAACTCA70.10300176574455562No Hit
TTTAGGGGGTCACATATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT70.10300176574455562No Hit
TTTAGGGGGTCACGGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC70.10300176574455562No Hit
TTTAGGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGAATTGAACAAAAACTC70.10300176574455562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCCA251.6944618E-550.83333237
CCAATCC251.6944618E-550.83333241
TGTCCAA251.6944618E-550.83333238
AATCCGT150.007787912650.4511343
TTTTGTC251.7733835E-550.45112635
TTTGTCC251.7733835E-550.45112636
GCCCAAA203.8624115E-450.07462748
TTCGTGG150.00802467550.07462715
AATTCGT150.00802467550.07462713
CACAAAA203.8624115E-450.07462747
GAAGCAG203.8624115E-450.0746279
CCGTCCA150.00802467550.07462746
AGCAGTG203.8624115E-450.07462711
GCAGTGG150.00802467550.07462712
ATTCGTG150.00802467550.07462714
GAATTCG150.00802467550.07462712
GGGAATT203.8624115E-450.07462710
GGAATTC203.8624115E-450.07462711
CGTCCAA150.00802467550.07462747
AAGCAGT203.8624115E-450.07462710
Produced by FastQC (version 0.11.5)